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NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK960146
Clone id TST39A01NGRL0006_J24
Library
Length 526
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0006_J24. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID -
Sequence 
GTGATATAATATCCTACTGTCTGTGTACCTGCTACGAACCTAAAACCGGAAAGGTGGTTT
TCATATAAGTATTCTTGCGGAGGGACCCACTCTCCTTTTGAACTATCCAAATTTTGTTTT
GCATCCGAAGAAACATCATTGTGGAATCTACTCTAGGTTACCTCCTACTCGCAATATTGA
CTAATAGGTAGACCGTGCTTTTACATTCAACTGCTGCTGCACATCTCTCAGCTTTTCTGT
CCTTGACATATCTTCGCATGAAATCCTATTTGCCATTGCCCCATATGTCACATTGCAGCT
GTTGCAGAATCCACATGAACAAAATTTGAACTTAAGTGTCTCTCATATTCATTTTGATAA
TCAACTCCGACTTGCTCAATATCCCGTCATGTTTCACGCTGGTGTGTGCAGCGTTGCTGA
TTCTGCCCTTGCACATGCAAGATGGATACTTAGAGAGGATCCTCAAGAAGTCATCAGAGT
TTTTTCAGATCAATAAACTATTACCTTAATGATTGCCTCGTGGAGG
■■Homology search results ■■ -
sp_hit_id Q54KX3
Definition sp|Q54KX3|VP13F_DICDI Putative vacuolar protein sorting-associated protein 13F OS=Dictyostelium discoideum
Align length 65
Score (bit) 42.7
E-value 0.001
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK960146|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0006_J24, 5'
         (526 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q54KX3|VP13F_DICDI Putative vacuolar protein sorting-associat...    43   0.001
sp|Q54LB8|VP13A_DICDI Putative vacuolar protein sorting-associat...    35   0.22 
sp|Q8BX70|VP13C_MOUSE Vacuolar protein sorting-associated protei...    32   1.4  
sp|Q43992|POBR_ACIAD P-hydroxybenzoate hydroxylase transcription...    32   2.4  
sp|Q54LN2|VP13D_DICDI Putative vacuolar protein sorting-associat...    31   3.2  
sp|Q709C8|VP13C_HUMAN Vacuolar protein sorting-associated protei...    31   3.2  
sp|P57272|ACKA_BUCAI Acetate kinase OS=Buchnera aphidicola subsp...    31   3.2  
sp|P24485|CD53_RAT Leukocyte surface antigen CD53 OS=Rattus norv...    30   9.2  

>sp|Q54KX3|VP13F_DICDI Putative vacuolar protein sorting-associated
            protein 13F OS=Dictyostelium discoideum GN=vps13F PE=3
            SV=1
          Length = 4246

 Score = 42.7 bits (99), Expect = 0.001
 Identities = 22/65 (33%), Positives = 33/65 (50%)
 Frame = +2

Query: 206  FNCCCTSLSFSVLDISSHEILFAIAPYVTLQLLQNPHEQNLNLSVSHIHFDNQLRLAQYP 385
            F  C   +S S++D    E+++     + L+  Q   EQN+NLS+     DNQL    YP
Sbjct: 3658 FEICIGGISVSLIDGHPKELMYITLRGLQLRFKQYSTEQNINLSIHSFKVDNQLYHTPYP 3717

Query: 386  VMFHA 400
            VM  +
Sbjct: 3718 VMIQS 3722


>sp|Q54LB8|VP13A_DICDI Putative vacuolar protein sorting-associated
            protein 13A OS=Dictyostelium discoideum GN=vps13A PE=2
            SV=1
          Length = 3373

 Score = 35.0 bits (79), Expect = 0.22
 Identities = 15/66 (22%), Positives = 32/66 (48%)
 Frame = +2

Query: 200  FTFNCCCTSLSFSVLDISSHEILFAIAPYVTLQLLQNPHEQNLNLSVSHIHFDNQLRLAQ 379
            + FN  C+ +  S++D +  E+ +       +   Q+  E  + + ++ +  DNQL    
Sbjct: 2782 YHFNIRCSGIGCSIIDKTPKELAYISMKDFLIIATQSSIENTVEVKLAELQIDNQLIKTD 2841

Query: 380  YPVMFH 397
            +PV+ H
Sbjct: 2842 FPVLIH 2847


>sp|Q8BX70|VP13C_MOUSE Vacuolar protein sorting-associated protein 13C
            OS=Mus musculus GN=Vps13c PE=2 SV=2
          Length = 3748

 Score = 32.3 bits (72), Expect = 1.4
 Identities = 12/37 (32%), Positives = 23/37 (62%)
 Frame = +2

Query: 287  VTLQLLQNPHEQNLNLSVSHIHFDNQLRLAQYPVMFH 397
            + ++  Q+PH+++L   +  +  DNQL    +PV+FH
Sbjct: 3182 IQVEFKQSPHQRSLRARLYWLQVDNQLPGTMFPVVFH 3218


>sp|Q43992|POBR_ACIAD P-hydroxybenzoate hydroxylase transcriptional
           activator OS=Acinetobacter sp. (strain ADP1) GN=pobR
           PE=1 SV=1
          Length = 271

 Score = 31.6 bits (70), Expect = 2.4
 Identities = 16/52 (30%), Positives = 27/52 (51%)
 Frame = +2

Query: 212 CCCTSLSFSVLDISSHEILFAIAPYVTLQLLQNPHEQNLNLSVSHIHFDNQL 367
           C  TSL+FS++ +  HE++     Y+       P + NL +S   +H  N+L
Sbjct: 110 CAQTSLTFSIVVLDEHEVVPVARSYL-------PQQDNLRVSPYGMHLGNRL 154


>sp|Q54LN2|VP13D_DICDI Putative vacuolar protein sorting-associated
            protein 13D OS=Dictyostelium discoideum GN=vps13D PE=3
            SV=1
          Length = 4592

 Score = 31.2 bits (69), Expect = 3.2
 Identities = 15/57 (26%), Positives = 30/57 (52%)
 Frame = +2

Query: 227  LSFSVLDISSHEILFAIAPYVTLQLLQNPHEQNLNLSVSHIHFDNQLRLAQYPVMFH 397
            +  S++D +S E+++     ++L    +       L V+++  DNQL    YPV+F+
Sbjct: 3980 IGVSLIDHNSKELIYLSLNDISLSFSLSNIFSRFELIVTNMQIDNQLMNTDYPVLFY 4036


>sp|Q709C8|VP13C_HUMAN Vacuolar protein sorting-associated protein 13C
            OS=Homo sapiens GN=VPS13C PE=1 SV=1
          Length = 3753

 Score = 31.2 bits (69), Expect = 3.2
 Identities = 12/37 (32%), Positives = 23/37 (62%)
 Frame = +2

Query: 287  VTLQLLQNPHEQNLNLSVSHIHFDNQLRLAQYPVMFH 397
            + ++  Q+ H+++L   +  +  DNQL  A +PV+FH
Sbjct: 3187 IQIEFKQSSHQRSLRARLYWLQVDNQLPGAMFPVVFH 3223


>sp|P57272|ACKA_BUCAI Acetate kinase OS=Buchnera aphidicola subsp.
           Acyrthosiphon pisum GN=ackA PE=3 SV=1
          Length = 405

 Score = 31.2 bits (69), Expect = 3.2
 Identities = 16/54 (29%), Positives = 28/54 (51%), Gaps = 8/54 (14%)
 Frame = +2

Query: 209 NCCCTSLSFSVLD--------ISSHEILFAIAPYVTLQLLQNPHEQNLNLSVSH 346
           NC  +S+ F++L+          S E LF +  Y+T Q L   H++ +  +V+H
Sbjct: 13  NCGSSSIKFAILNPDNKEKYLSGSVECLFLLETYITWQCLGTKHKKKIGANVNH 66


>sp|P24485|CD53_RAT Leukocyte surface antigen CD53 OS=Rattus
           norvegicus GN=Cd53 PE=1 SV=3
          Length = 219

 Score = 29.6 bits (65), Expect = 9.2
 Identities = 22/72 (30%), Positives = 32/72 (44%), Gaps = 2/72 (2%)
 Frame = +2

Query: 218 CTSLSFSVLD--ISSHEILFAIAPYVTLQLLQNPHEQNLNLSVSHIHFDNQLRLAQYPVM 391
           C  +SF VL   I   E+  AI  +V  + +     + LN S+ H H DN  R+A   + 
Sbjct: 80  CLLMSFFVLLLLILLAEVTLAILLFVYEKKINTLVAEGLNDSIQHYHSDNSTRMAWDFIQ 139

Query: 392 FHAGVCSVADSA 427
                C V  S+
Sbjct: 140 SQLQCCGVNGSS 151
tr_hit_id A9TKT0
Definition tr|A9TKT0|A9TKT0_PHYPA Predicted protein OS=Physcomitrella patens subsp. patens
Align length 172
Score (bit) 54.3
E-value 4.0e-06
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK960146|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0006_J24, 5'
         (526 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|A9TKT0|A9TKT0_PHYPA Predicted protein OS=Physcomitrella paten...    54   4e-06
tr|Q9FT44|Q9FT44_ARATH VPS13-like protein OS=Arabidopsis thalian...    44   0.004
tr|A2Q3V3|A2Q3V3_MEDTR C-5 cytosine-specific DNA methylase; Vacu...    43   0.009
tr|A7PSP9|A7PSP9_VITVI Chromosome chr6 scaffold_28, whole genome...    39   0.21 
tr|A5BWU7|A5BWU7_VITVI Putative uncharacterized protein OS=Vitis...    39   0.21 
tr|B4MXM9|B4MXM9_DROWI GK18894 OS=Drosophila willistoni GN=GK188...    37   0.61 
tr|B3NCU4|B3NCU4_DROER GG15595 OS=Drosophila erecta GN=GG15595 P...    36   1.0  
tr|B4PGU5|B4PGU5_DROYA GE21922 OS=Drosophila yakuba GN=GE21922 P...    36   1.4  
tr|B4QRR2|B4QRR2_DROSI GD14402 OS=Drosophila simulans GN=GD14402...    35   1.8  
tr|B4HG59|B4HG59_DROSE GM25370 OS=Drosophila sechellia GN=GM2537...    35   1.8  
tr|Q0YTB6|Q0YTB6_9CHLB RND efflux system, outer membrane lipopro...    35   2.3  
tr|Q9VU08|Q9VU08_DROME CG32113 OS=Drosophila melanogaster GN=CG3...    35   2.3  
tr|Q0U1E3|Q0U1E3_PHANO Putative uncharacterized protein OS=Phaeo...    35   3.0  
tr|B8BAF9|B8BAF9_ORYSI Putative uncharacterized protein OS=Oryza...    34   4.0  
tr|A3BP25|A3BP25_ORYSJ Putative uncharacterized protein OS=Oryza...    34   4.0  
tr|Q1LCX5|Q1LCX5_RALME Acyl-CoA dehydrogenase-like protein OS=Ra...    34   5.2  
tr|B4LGG3|B4LGG3_DROVI GJ11500 OS=Drosophila virilis GN=GJ11500 ...    33   6.7  

>tr|A9TKT0|A9TKT0_PHYPA Predicted protein OS=Physcomitrella patens
            subsp. patens GN=PHYPADRAFT_94056 PE=4 SV=1
          Length = 3667

 Score = 54.3 bits (129), Expect = 4e-06
 Identities = 47/172 (27%), Positives = 72/172 (41%), Gaps = 16/172 (9%)
 Frame = +2

Query: 59   FHISILAEGPTLLLNYPNFVLHPKKHHCGIYSRLPPTRNID**VDRAFTFNCCCTS---- 226
            F +S+ AEGP  +L+  +  LH  K   G  S     +     +          T     
Sbjct: 2946 FTVSVRAEGPIRVLSIVDTALHLMKSPSGSSSIFTSNQPFAGLIGPILESTVTGTEPVKE 3005

Query: 227  -------LSFSVLDISSHEILFAIAPYVTLQLLQNPHEQNLNLSVSHIHFDNQLRLAQYP 385
                   +  S++D    E++FA A  ++LQL+Q  H+Q L L V  I  DNQL  + YP
Sbjct: 3006 LILDFSYIGVSLIDSIPQEVIFACAKGLSLQLIQGQHQQQLKLEVCVIQIDNQLPFSVYP 3065

Query: 386  VMF-----HAGVCSVADSALAHARWILREDPQEVIRVFSDQ*TITLMIASWR 526
            V+       A +  V ++A A       E P           ++TL++A WR
Sbjct: 3066 VLLSSTYPQAKLGDVVNNAQAGEELKPLEGP-----------SLTLLVAKWR 3106


>tr|Q9FT44|Q9FT44_ARATH VPS13-like protein OS=Arabidopsis thaliana
            GN=At5g24740 PE=2 SV=1
          Length = 3306

 Score = 44.3 bits (103), Expect = 0.004
 Identities = 29/111 (26%), Positives = 54/111 (48%), Gaps = 3/111 (2%)
 Frame = +2

Query: 203  TFNCCCTSLSFSVLDISSHEILFAIAPYVTLQLLQNPHEQNLNLSVSHIHFDNQLRLAQY 382
            TF     S+  S+++    E+++A A  V L+L Q+  +Q L+  +S +  DN L+ + Y
Sbjct: 2638 TFLLVLPSIGISLVNSHPQELVYACASNVVLELSQSVDQQKLSFQISSLQIDNPLQNSSY 2697

Query: 383  PVMF---HAGVCSVADSALAHARWILREDPQEVIRVFSDQ*TITLMIASWR 526
            PV+    H       D  + + + IL  +  + +R  S    + + +A WR
Sbjct: 2698 PVILSFNHDHEVIPPDWGMKNNKAILLSEIVQQVRGNSCDAVVYVDLAKWR 2748


>tr|A2Q3V3|A2Q3V3_MEDTR C-5 cytosine-specific DNA methylase; Vacuolar
            protein sorting-associated protein OS=Medicago truncatula
            GN=MtrDRAFT_AC155889g36v2 PE=4 SV=1
          Length = 1694

 Score = 43.1 bits (100), Expect = 0.009
 Identities = 21/55 (38%), Positives = 32/55 (58%)
 Frame = +2

Query: 227  LSFSVLDISSHEILFAIAPYVTLQLLQNPHEQNLNLSVSHIHFDNQLRLAQYPVM 391
            +  S+++    E+LFA    + + LLQ+   Q L++ +S I  DNQLR   YPVM
Sbjct: 1020 IGISLINSYPQELLFACINDIQINLLQSLDRQRLSMRISFIQIDNQLRSTPYPVM 1074


>tr|A7PSP9|A7PSP9_VITVI Chromosome chr6 scaffold_28, whole genome
            shotgun sequence OS=Vitis vinifera GN=GSVIVT00023025001
            PE=4 SV=1
          Length = 1589

 Score = 38.5 bits (88), Expect = 0.21
 Identities = 18/55 (32%), Positives = 28/55 (50%)
 Frame = +2

Query: 227  LSFSVLDISSHEILFAIAPYVTLQLLQNPHEQNLNLSVSHIHFDNQLRLAQYPVM 391
            +  S++     E+LFA A    + LLQ+   Q  +  +S +  DNQL    YPV+
Sbjct: 928  IGISLISSYPQELLFACAKNTRIDLLQSLDHQKFSFQISSLQIDNQLHTTPYPVI 982


>tr|A5BWU7|A5BWU7_VITVI Putative uncharacterized protein OS=Vitis
           vinifera GN=VITISV_010429 PE=4 SV=1
          Length = 879

 Score = 38.5 bits (88), Expect = 0.21
 Identities = 18/55 (32%), Positives = 28/55 (50%)
 Frame = +2

Query: 227 LSFSVLDISSHEILFAIAPYVTLQLLQNPHEQNLNLSVSHIHFDNQLRLAQYPVM 391
           +  S++     E+LFA A    + LLQ+   Q  +  +S +  DNQL    YPV+
Sbjct: 208 IGISLISSYPQELLFACAKNTRIDLLQSLDHQKFSFQISSLQIDNQLHTTPYPVV 262


>tr|B4MXM9|B4MXM9_DROWI GK18894 OS=Drosophila willistoni GN=GK18894
            PE=4 SV=1
          Length = 3964

 Score = 37.0 bits (84), Expect = 0.61
 Identities = 18/46 (39%), Positives = 28/46 (60%)
 Frame = +2

Query: 260  EILFAIAPYVTLQLLQNPHEQNLNLSVSHIHFDNQLRLAQYPVMFH 397
            EI+F     ++  ++QN  E++LNL++S+I  DNQL  A   V  H
Sbjct: 3433 EIMFISLDRISCDIIQNSLEKSLNLNISYIQIDNQLLDAVNQVALH 3478


>tr|B3NCU4|B3NCU4_DROER GG15595 OS=Drosophila erecta GN=GG15595 PE=4
            SV=1
          Length = 3890

 Score = 36.2 bits (82), Expect = 1.0
 Identities = 17/54 (31%), Positives = 30/54 (55%)
 Frame = +2

Query: 260  EILFAIAPYVTLQLLQNPHEQNLNLSVSHIHFDNQLRLAQYPVMFHAGVCSVAD 421
            EI+F    ++   ++Q+  E +L+L++++I  DNQL  A  P+  H    S  D
Sbjct: 3358 EIMFISLDHINCDIVQSALENSLDLNIAYIQIDNQLLDAASPIALHTQTSSDYD 3411


>tr|B4PGU5|B4PGU5_DROYA GE21922 OS=Drosophila yakuba GN=GE21922 PE=4
            SV=1
          Length = 3890

 Score = 35.8 bits (81), Expect = 1.4
 Identities = 16/51 (31%), Positives = 29/51 (56%)
 Frame = +2

Query: 260  EILFAIAPYVTLQLLQNPHEQNLNLSVSHIHFDNQLRLAQYPVMFHAGVCS 412
            EI+F    ++   ++Q+  E +L+L++++I  DNQL  A  P+  H    S
Sbjct: 3358 EIMFISLDHINCDIVQSALENSLDLNIAYIQIDNQLLDAASPIALHTHTSS 3408


>tr|B4QRR2|B4QRR2_DROSI GD14402 OS=Drosophila simulans GN=GD14402 PE=4
            SV=1
          Length = 3892

 Score = 35.4 bits (80), Expect = 1.8
 Identities = 15/46 (32%), Positives = 28/46 (60%)
 Frame = +2

Query: 260  EILFAIAPYVTLQLLQNPHEQNLNLSVSHIHFDNQLRLAQYPVMFH 397
            EI+F    ++   ++Q+  E +L+L++++I  DNQL  A  P+  H
Sbjct: 3360 EIMFISLDHINCDIVQSALENSLDLNIAYIQIDNQLLDAASPIALH 3405


>tr|B4HG59|B4HG59_DROSE GM25370 OS=Drosophila sechellia GN=GM25370
            PE=4 SV=1
          Length = 3698

 Score = 35.4 bits (80), Expect = 1.8
 Identities = 15/46 (32%), Positives = 28/46 (60%)
 Frame = +2

Query: 260  EILFAIAPYVTLQLLQNPHEQNLNLSVSHIHFDNQLRLAQYPVMFH 397
            EI+F    ++   ++Q+  E +L+L++++I  DNQL  A  P+  H
Sbjct: 3166 EIMFISLDHINCDIVQSALENSLDLNIAYIQIDNQLLDAASPIALH 3211