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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK960235
Clone id TST39A01NGRL0006_N20
Library
Length 621
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0006_N20. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence 
CGCGACTGGTTCGAATCCAGTCCATCCTGGGCCTGATAGACTGAATGACATATTTACAAA
AGAGAGGGAAGTGACCGTGGATGTAAGTAGAAGGGCATTTGATGGAAAAAGAGAGAGTGA
AATGGTAGATATAAAGGCAGATCCACCGCGAATAATAGTAAATGGAAGGATGGAAAGAGA
GGAGGGAGAACTTTCACCTTCTCCGGAAGCAGATGGAAGGCTACCCGAAGGTTTTAATGG
AGGGCCTTCAATGGACTCTATTCCGACAGCTGTAAAGCAAGATGGTGTTGTTAGTCATGG
TTTCGGTGGTCATGCGGAGGAGGATGAGGAGATGGAATGCGATGGAAAGGGGGAGGGTGA
GCATGATGCAGATGCGGACGATGAAGGGGAGGAGAGTGCACCAAAATCATTCGATGATAG
TGAGAATCCATCAGAGGCTGGCGAGGAAGCTTCTGGTTCCGAGTCCGGTAATGCGGAGGA
TTCGCGAGAAGATGATGAAGATGAGCAGGAAGTCAAGAACGAGAGTGAAGGTGAAGCTGA
AGGAATGGCAGATGCTGAAGGTGAAGCAATATCTTTGGCTTCTTCAGAGAAGTTGTTGCA
CTCATGCATGCCTCTTGCAGC
■■Homology search results ■■ -
sp_hit_id Q8UJ51
Definition sp|Q8UJ51|IF2_AGRT5 Translation initiation factor IF-2 OS=Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Align length 75
Score (bit) 32.7
E-value 1.6
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK960235|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0006_N20, 5'
         (621 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8UJ51|IF2_AGRT5 Translation initiation factor IF-2 OS=Agroba...    33   1.6  
sp|Q39H30|IF2_BURS3 Translation initiation factor IF-2 OS=Burkho...    32   2.6  
sp|A0K6X5|IF2_BURCH Translation initiation factor IF-2 OS=Burkho...    32   2.6  
sp|Q1BWS7|IF2_BURCA Translation initiation factor IF-2 OS=Burkho...    32   2.6  
sp|P22227|ZFP42_MOUSE Zinc finger protein 42 OS=Mus musculus GN=...    32   3.5  
sp|A6UF29|IF2_SINMW Translation initiation factor IF-2 OS=Sinorh...    32   3.5  
sp|Q8TAV3|CP2W1_HUMAN Cytochrome P450 2W1 OS=Homo sapiens GN=CYP...    31   4.5  
sp|Q92SW4|IF2_RHIME Translation initiation factor IF-2 OS=Rhizob...    31   4.5  
sp|O96002|CX001_HUMAN Uncharacterized protein CXorf1 OS=Homo sap...    31   4.5  
sp|Q8FQ31|RF1_COREF Peptide chain release factor 1 OS=Corynebact...    31   5.9  
sp|Q92794|MYST3_HUMAN Histone acetyltransferase MYST3 OS=Homo sa...    31   5.9  
sp|Q2KDZ5|IF2_RHIEC Translation initiation factor IF-2 OS=Rhizob...    31   5.9  
sp|Q9W596|FUTSC_DROME Microtubule-associated protein futsch OS=D...    31   5.9  
sp|Q923I8|CRY2_RAT Cryptochrome-2 OS=Rattus norvegicus GN=Cry2 P...    30   7.7  
sp|Q9R194|CRY2_MOUSE Cryptochrome-2 OS=Mus musculus GN=Cry2 PE=1...    30   7.7  
sp|Q49AN0|CRY2_HUMAN Cryptochrome-2 OS=Homo sapiens GN=CRY2 PE=1...    30   7.7  

>sp|Q8UJ51|IF2_AGRT5 Translation initiation factor IF-2
           OS=Agrobacterium tumefaciens (strain C58 / ATCC 33970)
           GN=infB PE=3 SV=1
          Length = 913

 Score = 32.7 bits (73), Expect = 1.6
 Identities = 20/75 (26%), Positives = 38/75 (50%)
 Frame = +3

Query: 336 NAMERGRVSMMQMRTMKGRRVHQNHSMIVRIHQRLARKLLVPSPVMRRIREKMMKMSRKS 515
           N  E G      M +M+ R+     S +    +++ R++++P  +   I+E   +MS ++
Sbjct: 294 NLEEDGTPRGRSMASMRRRQEKFRRSQMQETREKVLREVILPETIT--IQELSQRMSERA 351

Query: 516 RTRVKVKLKEWQMLK 560
              +K  +KE QMLK
Sbjct: 352 VDVIKFLMKEGQMLK 366


>sp|Q39H30|IF2_BURS3 Translation initiation factor IF-2
           OS=Burkholderia sp. (strain 383) GN=infB PE=3 SV=1
          Length = 972

 Score = 32.0 bits (71), Expect = 2.6
 Identities = 21/64 (32%), Positives = 33/64 (51%)
 Frame = +3

Query: 384 KGRRVHQNHSMIVRIHQRLARKLLVPSPVMRRIREKMMKMSRKSRTRVKVKLKEWQMLKV 563
           KGR  HQ+ S      + + R++ VP  V   + +   KMS K+   +KV +K  QM+ +
Sbjct: 370 KGRGRHQDSSTFQAPTEPIVREVHVPETVS--VADLAHKMSVKASEVIKVMMKMGQMVTI 427

Query: 564 KQYL 575
            Q L
Sbjct: 428 NQVL 431


>sp|A0K6X5|IF2_BURCH Translation initiation factor IF-2
           OS=Burkholderia cenocepacia (strain HI2424) GN=infB PE=3
           SV=1
          Length = 971

 Score = 32.0 bits (71), Expect = 2.6
 Identities = 21/64 (32%), Positives = 33/64 (51%)
 Frame = +3

Query: 384 KGRRVHQNHSMIVRIHQRLARKLLVPSPVMRRIREKMMKMSRKSRTRVKVKLKEWQMLKV 563
           KGR  HQ+ S      + + R++ VP  V   + +   KMS K+   +KV +K  QM+ +
Sbjct: 369 KGRGRHQDSSTFQAPTEPIVREVHVPETVS--VADLAHKMSIKASEVIKVMMKMGQMVTI 426

Query: 564 KQYL 575
            Q L
Sbjct: 427 NQVL 430


>sp|Q1BWS7|IF2_BURCA Translation initiation factor IF-2
           OS=Burkholderia cenocepacia (strain AU 1054) GN=infB
           PE=3 SV=1
          Length = 971

 Score = 32.0 bits (71), Expect = 2.6
 Identities = 21/64 (32%), Positives = 33/64 (51%)
 Frame = +3

Query: 384 KGRRVHQNHSMIVRIHQRLARKLLVPSPVMRRIREKMMKMSRKSRTRVKVKLKEWQMLKV 563
           KGR  HQ+ S      + + R++ VP  V   + +   KMS K+   +KV +K  QM+ +
Sbjct: 369 KGRGRHQDSSTFQAPTEPIVREVHVPETVS--VADLAHKMSIKASEVIKVMMKMGQMVTI 426

Query: 564 KQYL 575
            Q L
Sbjct: 427 NQVL 430


>sp|P22227|ZFP42_MOUSE Zinc finger protein 42 OS=Mus musculus
           GN=Zfp42 PE=2 SV=1
          Length = 288

 Score = 31.6 bits (70), Expect = 3.5
 Identities = 20/67 (29%), Positives = 33/67 (49%), Gaps = 8/67 (11%)
 Frame = +2

Query: 59  KEREVTVDVSRRAFDGKRESEMVDIKADPPRIIVNGRME--------REEGELSPSPEAD 214
           ++ + T   S +   G R  + + +K D  R + N R+E        R+E E+SP  E D
Sbjct: 8   EQMKKTAKTSGQKGPGGRALDRLTLKQDEARPVQNTRVEAPRVTYTIRDESEISPETEED 67

Query: 215 GRLPEGF 235
           G  P+G+
Sbjct: 68  G-FPDGY 73


>sp|A6UF29|IF2_SINMW Translation initiation factor IF-2
           OS=Sinorhizobium medicae (strain WSM419) GN=infB PE=3
           SV=1
          Length = 885

 Score = 31.6 bits (70), Expect = 3.5
 Identities = 21/73 (28%), Positives = 42/73 (57%), Gaps = 1/73 (1%)
 Frame = +3

Query: 345 ERGR-VSMMQMRTMKGRRVHQNHSMIVRIHQRLARKLLVPSPVMRRIREKMMKMSRKSRT 521
           +RGR +S M+ R  K +R     S +    ++++R++++P  +   I+E   +MS ++  
Sbjct: 273 QRGRSLSAMRRRQEKFKR-----SQMQETREKISREVILPETIT--IQELSQRMSERAVD 325

Query: 522 RVKVKLKEWQMLK 560
            +K  +KE QM+K
Sbjct: 326 VIKFLMKEGQMMK 338


>sp|Q8TAV3|CP2W1_HUMAN Cytochrome P450 2W1 OS=Homo sapiens GN=CYP2W1
           PE=2 SV=2
          Length = 490

 Score = 31.2 bits (69), Expect = 4.5
 Identities = 14/28 (50%), Positives = 19/28 (67%)
 Frame = +1

Query: 175 KRGGRTFTFSGSRWKATRRF*WRAFNGL 258
           +RGG  F  SG+RW+A R+F  RA + L
Sbjct: 109 QRGGGIFFSSGARWRAARQFTVRALHSL 136


>sp|Q92SW4|IF2_RHIME Translation initiation factor IF-2 OS=Rhizobium
           meliloti GN=infB PE=3 SV=1
          Length = 889

 Score = 31.2 bits (69), Expect = 4.5
 Identities = 21/73 (28%), Positives = 42/73 (57%), Gaps = 1/73 (1%)
 Frame = +3

Query: 345 ERGR-VSMMQMRTMKGRRVHQNHSMIVRIHQRLARKLLVPSPVMRRIREKMMKMSRKSRT 521
           +RGR +S M+ R  K +R     S +    ++++R++++P  +   I+E   +MS ++  
Sbjct: 277 QRGRSLSAMRRRQEKFKR-----SQMQETREKISREVVLPETIT--IQELSQRMSERAVD 329

Query: 522 RVKVKLKEWQMLK 560
            +K  +KE QM+K
Sbjct: 330 VIKFLMKEGQMMK 342


>sp|O96002|CX001_HUMAN Uncharacterized protein CXorf1 OS=Homo
           sapiens GN=CXorf1 PE=2 SV=1
          Length = 111

 Score = 31.2 bits (69), Expect = 4.5
 Identities = 17/52 (32%), Positives = 26/52 (50%)
 Frame = -3

Query: 187 SLLSFHPSIYYYSRWICLYIYHFTLSFSIKCPSTYIHGHFPLFCKYVIQSIR 32
           ++  F   I+ +  WI L+ ++FT SF      TY H H  +   Y +QS R
Sbjct: 52  TIFYFRIFIFIFHSWILLFHFYFTCSF-----KTYEHQHSKMVPAYRMQSPR 98


>sp|Q8FQ31|RF1_COREF Peptide chain release factor 1
           OS=Corynebacterium efficiens GN=prfA PE=3 SV=1
          Length = 358

 Score = 30.8 bits (68), Expect = 5.9
 Identities = 19/59 (32%), Positives = 29/59 (49%), Gaps = 4/59 (6%)
 Frame = +2

Query: 20  VHPGPDRLNDIFTKEREVTVDVSRRAFDGKRESEMVD----IKADPPRIIVNGRMEREE 184
           V+P PD + D+   E+E+ VDV R +  G +     D    I   P  I+V  + ER +
Sbjct: 206 VYPEPDEVEDVEIDEKEIRVDVYRSSGKGGQGVNTTDSAVRITHLPTGIVVTCQKERSQ 264
tr_hit_id A5J8H8
Definition tr|A5J8H8|A5J8H8_BURMA Putative uncharacterized protein (Fragment) OS=Burkholderia mallei FMH
Align length 83
Score (bit) 37.0
E-value 0.95
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK960235|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0006_N20, 5'
         (621 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|A5J8H8|A5J8H8_BURMA Putative uncharacterized protein (Fragmen...    37   0.95 
tr|A1A5U9|A1A5U9_DANRE Si:ch211-69g19.2 protein OS=Danio rerio G...    37   1.2  
tr|B2H3Q1|B2H3Q1_BURPS Putative uncharacterized protein OS=Burkh...    35   3.6  
tr|Q1ME86|Q1ME86_RHIL3 Putative serine protease OS=Rhizobium leg...    34   6.1  
tr|B5ZWE1|B5ZWE1_RHILW Protease Do OS=Rhizobium leguminosarum bv...    34   6.1  
tr|A9CWK9|A9CWK9_9RHIZ Translation initiation factor IF-2 OS=Hoe...    34   7.9  
tr|A5KBC6|A5KBC6_PLAVI Phosphatidylinositol 3-and 4-kinase, puta...    34   7.9  

>tr|A5J8H8|A5J8H8_BURMA Putative uncharacterized protein (Fragment)
           OS=Burkholderia mallei FMH GN=BMAFMH_0485 PE=4 SV=1
          Length = 583

 Score = 37.0 bits (84), Expect = 0.95
 Identities = 28/83 (33%), Positives = 34/83 (40%), Gaps = 12/83 (14%)
 Frame = +1

Query: 103 WKKRE*NGRYKGRSTANNSKWKDGKR--GG---RTFTFSGSRWKATRRF*WRAFNGLYSD 267
           W  R   GR+ G  T    + + G+R  GG   R + F G+R    RRF WR F   Y  
Sbjct: 407 WDNRLDRGRHGGHRTRQLGRQRIGRRNVGGWCVRPWHF-GARCCGRRRFQWRGFGWRYFR 465

Query: 268 SCKARWCC*SW-------FRWSC 315
                W C  W       FRW C
Sbjct: 466 RRCFEWRCFRWRCFRRRRFRWRC 488


>tr|A1A5U9|A1A5U9_DANRE Si:ch211-69g19.2 protein OS=Danio rerio
           GN=si:ch211-69g19.2 PE=2 SV=1
          Length = 450

 Score = 36.6 bits (83), Expect = 1.2
 Identities = 22/71 (30%), Positives = 40/71 (56%), Gaps = 3/71 (4%)
 Frame = +3

Query: 348 RGRVSMMQMRTMKGRRVHQNHSMIVRIHQRLARKLLV---PSPVMRRIREKMMKMSRKSR 518
           RG+ ++   RT+K    ++N++M+VR  Q+   K+L+    SP ++ +RE      R S+
Sbjct: 362 RGKTAVSSPRTIKNTATNENNNMVVRTKQKPREKVLIGEGRSP-LQILRETNSPRERNSQ 420

Query: 519 TRVKVKLKEWQ 551
            ++KV   E Q
Sbjct: 421 LKLKVSTPERQ 431


>tr|B2H3Q1|B2H3Q1_BURPS Putative uncharacterized protein
           OS=Burkholderia pseudomallei 1655 GN=BURPS1655_K0408
           PE=4 SV=1
          Length = 479

 Score = 35.0 bits (79), Expect = 3.6
 Identities = 27/83 (32%), Positives = 33/83 (39%), Gaps = 12/83 (14%)
 Frame = +1

Query: 103 WKKRE*NGRYKGRSTANNSKWKDGKR--GG---RTFTFSGSRWKATRRF*WRAFNGLYSD 267
           W  R   GR+ G       + + G+R  GG   R + F G+R    RRF WR F   Y  
Sbjct: 129 WDNRLDRGRHGGHRARQLGRQRIGRRNVGGWCVRPWHF-GARCCGRRRFQWRGFGWRYFR 187

Query: 268 SCKARWCC*SW-------FRWSC 315
                W C  W       FRW C
Sbjct: 188 RRCFEWRCFRWRGFRRRRFRWRC 210


>tr|Q1ME86|Q1ME86_RHIL3 Putative serine protease OS=Rhizobium
           leguminosarum bv. viciae (strain 3841) GN=RL3252 PE=3
           SV=1
          Length = 551

 Score = 34.3 bits (77), Expect = 6.1
 Identities = 29/111 (26%), Positives = 45/111 (40%), Gaps = 7/111 (6%)
 Frame = +2

Query: 17  PVHPGPDRLNDIFTKEREVTVDVSRRAFDGKRESEMVDIKADPPRIIVNGRMERE----- 181
           PV  G  +  D+  K            FDGK  SEM D+    PR++    + +E     
Sbjct: 331 PVDDGSIKAGDVILK------------FDGKTVSEMRDL----PRVVAESTVGKEVDVVV 374

Query: 182 --EGELSPSPEADGRLPEGFNGGPSMDSIPTAVKQDGVVSHGFGGHAEEDE 328
             +G+        GRL +      S DS P   + DGV++   G + + D+
Sbjct: 375 LRDGKEQTVKVKLGRLEDSDQAAASGDSAPDGSQDDGVITPDPGENNDMDQ 425


>tr|B5ZWE1|B5ZWE1_RHILW Protease Do OS=Rhizobium leguminosarum bv.
           trifolii (strain WSM2304) GN=Rleg2_2539 PE=3 SV=1
          Length = 578

 Score = 34.3 bits (77), Expect = 6.1
 Identities = 29/111 (26%), Positives = 45/111 (40%), Gaps = 7/111 (6%)
 Frame = +2

Query: 17  PVHPGPDRLNDIFTKEREVTVDVSRRAFDGKRESEMVDIKADPPRIIVNGRMERE----- 181
           PV  G  +  D+  K            FDGK  SEM D+    PR++    + +E     
Sbjct: 356 PVDDGSIKAGDVILK------------FDGKTVSEMRDL----PRVVAESSVGKEVDVVV 399

Query: 182 --EGELSPSPEADGRLPEGFNGGPSMDSIPTAVKQDGVVSHGFGGHAEEDE 328
             +G+        GRL +      S D+ P   + DGV++   G + + DE
Sbjct: 400 LRDGKEQTVKVKLGRLEDSDQAAASGDAAPDGSQDDGVITPDPGENNDMDE 450


>tr|A9CWK9|A9CWK9_9RHIZ Translation initiation factor IF-2
           OS=Hoeflea phototrophica DFL-43 GN=infB PE=4 SV=1
          Length = 870

 Score = 33.9 bits (76), Expect = 7.9
 Identities = 24/72 (33%), Positives = 41/72 (56%), Gaps = 1/72 (1%)
 Frame = +3

Query: 348 RGR-VSMMQMRTMKGRRVHQNHSMIVRIHQRLARKLLVPSPVMRRIREKMMKMSRKSRTR 524
           RGR +S M+ R  K RR     SM+    +++AR++ +P  +   I+E   +M+ +S   
Sbjct: 258 RGRSLSAMRRRQEKMRR-----SMMQETREKIAREVTLPETIT--IQELSQRMAERSVDV 310

Query: 525 VKVKLKEWQMLK 560
           +K  +KE QM+K
Sbjct: 311 IKYLMKEGQMMK 322


>tr|A5KBC6|A5KBC6_PLAVI Phosphatidylinositol 3-and 4-kinase, putative
            OS=Plasmodium vivax GN=PVX_119670 PE=4 SV=1
          Length = 1032

 Score = 33.9 bits (76), Expect = 7.9
 Identities = 26/87 (29%), Positives = 37/87 (42%)
 Frame = +2

Query: 77   VDVSRRAFDGKRESEMVDIKADPPRIIVNGRMEREEGELSPSPEADGRLPEGFNGGPSMD 256
            V V R+    K++ +  D     P    + + E EEG+  PS + D    EG        
Sbjct: 826  VRVKRKKKKKKKKKKHFDKDGSKPG---DDKREDEEGKELPSDKPDDVPEEG-------- 874

Query: 257  SIPTAVKQDGVVSHGFGGHAEEDEEME 337
               T   Q G++  G  GH E+DEE E
Sbjct: 875  --QTGTAQGGLLKSGLQGHTEDDEEEE 899