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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK960440
Clone id TST39A01NGRL0007_G20
Library
Length 579
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0007_G20. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID -
Sequence 
CCCATTGCTACTTGTTGACCCAGAAAAATGCAGCGATGGAGATTTCTGCTTGCCTTGGTG
ACGATCTTGGCCCTTGGATTTCCTGCTGCGGATGCCCGCAGGATGCGCGAGGATTCGCCG
TCGGCGGTGATGCCCTGCAAGGTTGGCTGACTTTGAAAGTCATCGATATTCACAAGGAGC
ATGGGCAGAGTCTTGCCTTTTCGAGGAGCAAAGCAGTCCCCTTTTTCGGATGAATACCTG
GACTTCGACGCCTCGATGGTCCTCAGGTGCAAGGCTTTCCTCCGAGCTTTGTCTGCGATA
CATGTATGGATATTTCTACATTTGCCGAGCAAGTTCTGACAGATCCTGACACAACGAGCA
ACGTTCTAGACTGTGTCAATGTGTCTCTCTGTCCAGTTCTTCCTGCGTTTCAATCATAGT
GAGTTAACTTGAGCGAGATGTCTGTTCCTGGCTTGGTTGAGACCATGCATATATAGGCTG
CACCGCACTAACTTTGTGGAGAAACTAAACTTTGCCCGAGTACCGCCATGCTGCTACTTA
ATGATGCTCGGTCATGTAAGATGTGCACCCAGTTTGCCA
■■Homology search results ■■ -
sp_hit_id Q2PPJ7
Definition sp|Q2PPJ7|AS250_HUMAN 250 kDa substrate of Akt OS=Homo sapiens
Align length 49
Score (bit) 30.4
E-value 6.7
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK960440|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0007_G20, 5'
         (579 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q2PPJ7|AS250_HUMAN 250 kDa substrate of Akt OS=Homo sapiens G...    30   6.7  
sp|Q92A85|AROA_LISIN 3-phosphoshikimate 1-carboxyvinyltransferas...    30   6.7  
sp|Q8Y5Y0|AROA_LISMO 3-phosphoshikimate 1-carboxyvinyltransferas...    30   8.7  

>sp|Q2PPJ7|AS250_HUMAN 250 kDa substrate of Akt OS=Homo sapiens
            GN=C20orf74 PE=1 SV=2
          Length = 1873

 Score = 30.4 bits (67), Expect = 6.7
 Identities = 19/49 (38%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
 Frame = +3

Query: 174  KEHGQSLAFSRSKAVPFFG*IPGLRRLDGPQVQG-FPPSFVCDTCMDIS 317
            K H   +A ++   VPFFG +      DG  V G   PS VC TC++ S
Sbjct: 1776 KNHMFFIAITKKPEVPFFGPL-----FDGAIVSGKLLPSLVCATCINAS 1819


>sp|Q92A85|AROA_LISIN 3-phosphoshikimate 1-carboxyvinyltransferase
           OS=Listeria innocua GN=aroA PE=3 SV=1
          Length = 428

 Score = 30.4 bits (67), Expect = 6.7
 Identities = 27/86 (31%), Positives = 39/86 (45%), Gaps = 5/86 (5%)
 Frame = -3

Query: 520 LGQSLVSPQS*CGAAYICMVST-KPGTDISLKLTHYD*NAGRTGQRDTLTQSRTLLVVSG 344
           +GQ +  P     AA+  +     PG++I  +LTH   N  RTG  D + Q    LVV  
Sbjct: 223 IGQEMTVPGDVSSAAFFIVAGLITPGSEI--ELTHVGLNPTRTGIFDVVEQMGGSLVVKD 280

Query: 343 SVRT----CSANVEISIHVSQTKLGG 278
           S R+        V  S  +  T++GG
Sbjct: 281 SSRSTGKLAGTVVVKSSELKGTEIGG 306


>sp|Q8Y5Y0|AROA_LISMO 3-phosphoshikimate 1-carboxyvinyltransferase
           OS=Listeria monocytogenes GN=aroA PE=3 SV=1
          Length = 428

 Score = 30.0 bits (66), Expect = 8.7
 Identities = 26/81 (32%), Positives = 39/81 (48%), Gaps = 1/81 (1%)
 Frame = -3

Query: 517 GQSLVSPQS*CGAAYICMVST-KPGTDISLKLTHYD*NAGRTGQRDTLTQSRTLLVVSGS 341
           GQ +  P     AA+  +     PG++I  +LTH   N  RTG  D + Q    LVV  S
Sbjct: 224 GQEMTVPGDVSSAAFFIVAGLITPGSEI--ELTHVGLNPTRTGIFDVVKQMGGSLVVKDS 281

Query: 340 VRTCSANVEISIHVSQTKLGG 278
            R+ +  +  ++ V  +KL G
Sbjct: 282 SRS-TGKLAGTVVVKTSKLKG 301
tr_hit_id A9P228
Definition tr|A9P228|A9P228_PICSI Putative uncharacterized protein OS=Picea sitchensis
Align length 183
Score (bit) 45.8
E-value 0.002
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK960440|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0007_G20, 5'
         (579 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|A9P228|A9P228_PICSI Putative uncharacterized protein OS=Picea...    46   0.002
tr|A9NUE1|A9NUE1_PICSI Putative uncharacterized protein OS=Picea...    45   0.002
tr|A8I478|A8I478_CHLRE Predicted protein OS=Chlamydomonas reinha...    44   0.005
tr|A8FVR1|A8FVR1_SHESH Methyl-accepting chemotaxis sensory trans...    36   1.8  
tr|B3MGK8|B3MGK8_DROAN GF12627 OS=Drosophila ananassae GN=GF1262...    35   2.3  
tr|A8J8E3|A8J8E3_CHLRE Predicted protein OS=Chlamydomonas reinha...    35   3.0  
tr|Q4JHB2|Q4JHB2_TRIVA Saposin-like protein 2 (Fragment) OS=Tric...    34   6.7  
tr|B6KPV2|B6KPV2_TOXGO Putative uncharacterized protein OS=Toxop...    34   6.7  
tr|A2FFY3|A2FFY3_TRIVA Surfactant B protein, putative OS=Trichom...    34   6.7  
tr|B2U829|B2U829_RALPJ Gluconate 2-dehydrogenase (Acceptor) OS=R...    33   8.7  

>tr|A9P228|A9P228_PICSI Putative uncharacterized protein OS=Picea
           sitchensis PE=2 SV=1
          Length = 326

 Score = 45.8 bits (107), Expect = 0.002
 Identities = 51/183 (27%), Positives = 75/183 (40%), Gaps = 24/183 (13%)
 Frame = +3

Query: 99  QDARGFAVGGDALQGWLTLKVIDIHKEHGQSLAFSRSKA---VPFFG*IP---GLRRLDG 260
           ++A+    GGD  +    L  ++      Q LA   SK    VP    +P    + R  G
Sbjct: 72  EEAQRVVFGGDMPESVPRLSRVN------QKLAEGLSKMLNIVPRLSEVPLEKTVSRSGG 125

Query: 261 PQVQGFPPSFVCDTCMDISTFAEQVLTDPDTTSNVLDCVNVSLCPVLPAFQS**VNLSEM 440
            +  G      C+TCM++S  AE+VL+DP+T  N +     S+C  LP+  S      EM
Sbjct: 126 LKFHGSSLGLFCNTCMEVSNQAEEVLSDPETLENAVKLAK-SICNELPSDLS--AKCDEM 182

Query: 441 ---SVPGLVETMHI*AAPH*LCGETKLC---------------PSTAMLLLNDARSCKMC 566
               +  +V T+    +   LC  T LC                +T  L   D  +C MC
Sbjct: 183 LGTYIQEVVSTLQDYLSQDKLCIGTGLCNGNNNNDLQYKLGMGRNTPSLDAGDDTTCVMC 242

Query: 567 TQF 575
            QF
Sbjct: 243 EQF 245


>tr|A9NUE1|A9NUE1_PICSI Putative uncharacterized protein OS=Picea
           sitchensis PE=2 SV=1
          Length = 430

 Score = 45.4 bits (106), Expect = 0.002
 Identities = 51/183 (27%), Positives = 75/183 (40%), Gaps = 24/183 (13%)
 Frame = +3

Query: 99  QDARGFAVGGDALQGWLTLKVIDIHKEHGQSLAFSRSKA---VPFFG*IP---GLRRLDG 260
           ++A+    GGD  +    L  ++      Q LA   SK    VP    +P    + R  G
Sbjct: 72  EEAQRVVFGGDMPESVPRLSRVN------QKLAEGLSKMLNIVPRLSEVPLEKTVSRSGG 125

Query: 261 PQVQGFPPSFVCDTCMDISTFAEQVLTDPDTTSNVLDCVNVSLCPVLPAFQS**VNLSEM 440
            +  G      C+TCM++S  AE+VL+DP+T  N +     S+C  LP+  S      EM
Sbjct: 126 LKFHGSSLGLFCNTCMEVSKQAEEVLSDPETLENAVKLAK-SICNELPSDLS--AKCDEM 182

Query: 441 ---SVPGLVETMHI*AAPH*LCGETKLC---------------PSTAMLLLNDARSCKMC 566
               +  +V T+    +   LC  T LC                +T  L   D  +C MC
Sbjct: 183 LGTYIQEVVSTLQDYLSQDKLCIGTGLCNGNNNNDLQYKLNMGRNTPSLDAGDDTTCVMC 242

Query: 567 TQF 575
            QF
Sbjct: 243 EQF 245


>tr|A8I478|A8I478_CHLRE Predicted protein OS=Chlamydomonas
           reinhardtii GN=CHLREDRAFT_186862 PE=4 SV=1
          Length = 462

 Score = 44.3 bits (103), Expect = 0.005
 Identities = 26/100 (26%), Positives = 46/100 (46%), Gaps = 9/100 (9%)
 Frame = +3

Query: 294 CDTCMDISTFAEQVLTDPDTTSNVLDCVNVSLCPVLPAFQS**VNLSEMSVPGLVETMHI 473
           C TC+ +    E +L DP  T  ++D V   +CP +    +   NL+E  +P +++ +  
Sbjct: 37  CQTCLLVVRIVEDLLCDPAATEFLVDLVEKQVCPAM-GDSAQCHNLAEGLLPTVIQWLRA 95

Query: 474 *AAPH*LCGETKLCPST---------AMLLLNDARSCKMC 566
            A P  LCG   +C +            L++ D+  C +C
Sbjct: 96  SATPASLCGGAGVCGAVLAQVPELNKPSLVVRDSTQCSLC 135


>tr|A8FVR1|A8FVR1_SHESH Methyl-accepting chemotaxis sensory
           transducer OS=Shewanella sediminis (strain HAW-EB3)
           GN=Ssed_2325 PE=3 SV=1
          Length = 664

 Score = 35.8 bits (81), Expect = 1.8
 Identities = 26/82 (31%), Positives = 43/82 (52%), Gaps = 5/82 (6%)
 Frame = +3

Query: 159 VIDIHKEHGQSLAFSRSKAVPFFG*IPGLRRLDGPQVQGFPPSFVCDTCMD--ISTFAEQ 332
           V ++ KE G S  F  SK   F G +PG R+L   Q+ GF  +FV D  +    ST   +
Sbjct: 61  VHELQKERGMSAGFLGSKGKSFAGKLPGQRQLTDKQLSGF-NTFVRDNTLPAVFSTQLSR 119

Query: 333 VLTDPDTTSNV---LDCVNVSL 389
           V ++    +N+   +D +++S+
Sbjct: 120 VNSEVSRLNNIRSGVDSLSISV 141


>tr|B3MGK8|B3MGK8_DROAN GF12627 OS=Drosophila ananassae GN=GF12627
           PE=4 SV=1
          Length = 196

 Score = 35.4 bits (80), Expect = 2.3
 Identities = 22/75 (29%), Positives = 42/75 (56%), Gaps = 4/75 (5%)
 Frame = +1

Query: 286 ALSAIHVWIFLHLPSKF*QILTQ-RATF*TVSMCLSVQF---FLRFNHSELT*ARCLFLA 453
           ALS+ ++WI LH  +   +I+ +  +TF T  + L +++   F+ +  SEL     + + 
Sbjct: 7   ALSSFYLWIMLHAAAIMPEIIERWESTFWTSRLKLFIRYKTVFISYQKSEL--IIYMLVT 64

Query: 454 WLRPCIYRLHRTNFV 498
           W+  C++R  R NF+
Sbjct: 65  WVMFCLFRCCRCNFL 79


>tr|A8J8E3|A8J8E3_CHLRE Predicted protein OS=Chlamydomonas
           reinhardtii GN=CHLREDRAFT_192781 PE=4 SV=1
          Length = 429

 Score = 35.0 bits (79), Expect = 3.0
 Identities = 21/75 (28%), Positives = 31/75 (41%)
 Frame = +3

Query: 291 VCDTCMDISTFAEQVLTDPDTTSNVLDCVNVSLCPVLPAFQS**VNLSEMSVPGLVETMH 470
           VCDTC+      E  L D    + V+D     LCP  P  +     L+E  +P  ++ + 
Sbjct: 50  VCDTCLIAMRLLEDALCDDGAVAFVVDLFEKQLCPATPD-KDECEQLAEAFIPVAMQWLR 108

Query: 471 I*AAPH*LCGETKLC 515
               P  LC    +C
Sbjct: 109 ASETPASLCAAVGVC 123


>tr|Q4JHB2|Q4JHB2_TRIVA Saposin-like protein 2 (Fragment)
           OS=Trichomonas vaginalis PE=2 SV=1
          Length = 533

 Score = 33.9 bits (76), Expect = 6.7
 Identities = 23/95 (24%), Positives = 42/95 (44%), Gaps = 1/95 (1%)
 Frame = +3

Query: 291 VCDTCMDISTFAEQVLTDPDTTSNVLDCVNVSLCPVLPA-FQS**VNLSEMSVPGLVETM 467
           +CD C+ +  + E+VL +    S V+   +   C  LPA F +   ++ E  VP +++ +
Sbjct: 104 ICDVCVSLIKYVEKVLLETKVESEVIALCD-KYCESLPAPFPTLCKSMVEKYVPVIIQYL 162

Query: 468 HI*AAPH*LCGETKLCPSTAMLLLNDARSCKMCTQ 572
                   +C +  LC S       +   C MC +
Sbjct: 163 EQGIEHLEICKKIGLCESVKGAKSQNGLICDMCVE 197


>tr|B6KPV2|B6KPV2_TOXGO Putative uncharacterized protein
           OS=Toxoplasma gondii ME49 GN=TGME49_118520 PE=4 SV=1
          Length = 801

 Score = 33.9 bits (76), Expect = 6.7
 Identities = 35/117 (29%), Positives = 45/117 (38%), Gaps = 3/117 (2%)
 Frame = -3

Query: 397 TGQRDTLTQSRTLLVVSGSVRTCSAN---VEISIHVSQTKLGGKPCT*GPSRRRSPGIHP 227
           TG     T S    VV GS +T SA    +      SQ++  G P         S G  P
Sbjct: 538 TGAGSGRTSSGPPSVVQGSEKTTSAGPTPMPAGDGESQSRTTGMPSA------DSLGEKP 591

Query: 226 KKGTALLLEKARLCPCSL*ISMTFKVSQPCRASPPTANPRASCGHPQQEIQGPRSSP 56
           +K + L     RL P    I+   +  Q    SPPT + +AS   P    Q P   P
Sbjct: 592 EKASELSQPLVRLAPRGGPITPVKEDVQHVSRSPPTPDEKASSAGPLGPSQSPEGEP 648


>tr|A2FFY3|A2FFY3_TRIVA Surfactant B protein, putative
           OS=Trichomonas vaginalis G3 GN=TVAG_000220 PE=4 SV=1
          Length = 534

 Score = 33.9 bits (76), Expect = 6.7
 Identities = 23/95 (24%), Positives = 42/95 (44%), Gaps = 1/95 (1%)
 Frame = +3

Query: 291 VCDTCMDISTFAEQVLTDPDTTSNVLDCVNVSLCPVLPA-FQS**VNLSEMSVPGLVETM 467
           +CD C+ +  + E+VL +    S V+   +   C  LPA F +   ++ E  VP +++ +
Sbjct: 105 ICDVCVSLIKYVEKVLLETKVESEVIALCD-KYCESLPAPFPTLCKSMVEKYVPVIIQYL 163

Query: 468 HI*AAPH*LCGETKLCPSTAMLLLNDARSCKMCTQ 572
                   +C +  LC S       +   C MC +
Sbjct: 164 EQGIEHLEICKKIGLCESVKGAKSQNGLICDMCVE 198


>tr|B2U829|B2U829_RALPJ Gluconate 2-dehydrogenase (Acceptor)
           OS=Ralstonia pickettii (strain 12J) GN=Rpic_0720 PE=3
           SV=1
          Length = 427

 Score = 33.5 bits (75), Expect = 8.7
 Identities = 29/109 (26%), Positives = 43/109 (39%), Gaps = 16/109 (14%)
 Frame = +3

Query: 87  CGCPQDARGFAV-------------GGDALQGWLTLKVI-DIHKEHGQSLAFSRSKAVPF 224
           CG     RGFA+              G  L GW    +  D     G   A+ R + V +
Sbjct: 191 CGTCHTPRGFAMQEKALDERGSGYLAGSTLAGWTAFNITSDPTSGIG---AWKREQLVQY 247

Query: 225 F--G*IPGLRRLDGPQVQGFPPSFVCDTCMDISTFAEQVLTDPDTTSNV 365
              G +PG+ +  GP  +    SF   T  D+   AE + T P  ++N+
Sbjct: 248 LRTGSVPGVAQAAGPMGEAVQHSFSKMTMQDVQAIAEYLRTVPAVSNNL 296