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NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK960714
Clone id TST39A01NGRL0008_C14
Library
Length 480
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0008_C14. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID -
Sequence 
AAATTCCAGACAGAGTTGTGCCAGGCGTATACAGGATACGACATTTTGGTGCCGCAAAGC
ATTTCTTTGGCTCTGTTCGCCACTTCATCGGAACAACCAAGTCCTTTGTGGTGTATTGAG
TTGAAGAAGAAAACAATTTTTGTACCGCTTTCTGATCAGCAGGTGTTAAAAAGGTTTGAG
CCTGTATAAACAAAAATGGGCTCACTATATAAATATGATGGTATTTCCTTGTAATTTATA
CAATAGTAATCAAAGCACTAGTGGGGATTGCTGATGTGTGCCCCTTGAATTATGACATCC
TTCATTTTGATGAGATTGTTGATGCTCTCAAAAGACTTGCGCCATTAGAGTGCAAGCTGT
TGTAATGCCTAAAGTATTAATGAGGCCATCTTTTTTGATTATTTGAGACTCTTAGTTTCA
TTCAATACAATGGCTGACACAGATTTGAGTNANACAAAAAAAAAAAAAAAAAAAAAAAAA
■■Homology search results ■■ -
sp_hit_id Q304B9
Definition sp|Q304B9|NCASE_ARATH Neutral ceramidase OS=Arabidopsis thaliana
Align length 37
Score (bit) 48.9
E-value 1.0e-05
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK960714|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0008_C14, 5'
         (455 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q304B9|NCASE_ARATH Neutral ceramidase OS=Arabidopsis thaliana...    49   1e-05
sp|Q29C43|NCASE_DROPS Neutral ceramidase OS=Drosophila pseudoobs...    34   0.26 
sp|Q9VA70|NCASE_DROME Neutral ceramidase OS=Drosophila melanogas...    33   0.44 
sp|Q9I596|NCASE_PSEAE Neutral ceramidase OS=Pseudomonas aerugino...    32   0.99 
sp|Q91XT9|ASAH2_RAT Neutral ceramidase OS=Rattus norvegicus GN=A...    31   2.2  
sp|Q89AV2|DYR_BUCBP Dihydrofolate reductase OS=Buchnera aphidico...    30   3.8  
sp|Q9JHE3|ASAH2_MOUSE Neutral ceramidase OS=Mus musculus GN=Asah...    30   4.9  
sp|Q9NR71|ASAH2_HUMAN Neutral ceramidase OS=Homo sapiens GN=ASAH...    30   6.4  
sp|P0C7U2|ASA2C_HUMAN Putative neutral ceramidase C OS=Homo sapi...    30   6.4  
sp|P0C7U1|ASA2B_HUMAN Putative inactive neutral ceramidase B OS=...    30   6.4  
sp|P19319|NARZ_ECOLI Respiratory nitrate reductase 2 alpha chain...    29   8.4  

>sp|Q304B9|NCASE_ARATH Neutral ceramidase OS=Arabidopsis thaliana
           GN=At2g38010 PE=2 SV=1
          Length = 757

 Score = 48.9 bits (115), Expect = 1e-05
 Identities = 20/37 (54%), Positives = 28/37 (75%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFGAAKHFFGSVRHFIGTTKSFVV 113
           +P+  V GVYRIRH+GA+K  FGS+  F G++ +FVV
Sbjct: 720 VPESAVAGVYRIRHYGASKSLFGSISSFSGSSSAFVV 756


>sp|Q29C43|NCASE_DROPS Neutral ceramidase OS=Drosophila
           pseudoobscura pseudoobscura GN=CDase PE=3 SV=1
          Length = 704

 Score = 34.3 bits (77), Expect = 0.26
 Identities = 16/37 (43%), Positives = 21/37 (56%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFGAAKHFFGSVRHFIGTTKSFVV 113
           I  + +PGVYRIRH G  K+  G    + G + SF V
Sbjct: 665 ISPQTLPGVYRIRHSGEYKYILGGKYPYEGLSHSFTV 701


>sp|Q9VA70|NCASE_DROME Neutral ceramidase OS=Drosophila melanogaster
           GN=CDase PE=1 SV=1
          Length = 704

 Score = 33.5 bits (75), Expect = 0.44
 Identities = 16/37 (43%), Positives = 20/37 (54%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFGAAKHFFGSVRHFIGTTKSFVV 113
           I  + +PG YRIRH G  K+  G    + G T SF V
Sbjct: 665 ISPQTLPGEYRIRHSGEYKYILGGKYPYEGLTHSFTV 701


>sp|Q9I596|NCASE_PSEAE Neutral ceramidase OS=Pseudomonas aeruginosa
           GN=PA0845 PE=1 SV=1
          Length = 670

 Score = 32.3 bits (72), Expect = 0.99
 Identities = 17/38 (44%), Positives = 23/38 (60%), Gaps = 1/38 (2%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFGAAKHFF-GSVRHFIGTTKSFVV 113
           IP    PG Y IRH+G AK+F+   +    G+T+SF V
Sbjct: 628 IPPGTEPGHYYIRHYGNAKNFWTQKISEIGGSTRSFEV 665


>sp|Q91XT9|ASAH2_RAT Neutral ceramidase OS=Rattus norvegicus
           GN=Asah2 PE=1 SV=1
          Length = 761

 Score = 31.2 bits (69), Expect = 2.2
 Identities = 12/16 (75%), Positives = 14/16 (87%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFG 50
           IPD  +PGVYRIR+FG
Sbjct: 718 IPDTALPGVYRIRYFG 733


>sp|Q89AV2|DYR_BUCBP Dihydrofolate reductase OS=Buchnera aphidicola
           subsp. Baizongia pistaciae GN=folA PE=3 SV=1
          Length = 164

 Score = 30.4 bits (67), Expect = 3.8
 Identities = 22/63 (34%), Positives = 34/63 (53%), Gaps = 4/63 (6%)
 Frame = -1

Query: 341 RKSFESINNLIKMKD--VIIQGAHISNPH*CFDYYCINY--KEIPSYLYSEPIFVYTGSN 174
           RK++ESI  ++ M+   V+ +  +I N +  F    +N   K I S LY   I +  GSN
Sbjct: 45  RKTWESIGRVLPMRQNIVLTRQKNIKNTNVLF----VNSISKAIQSALYKNEIMIIGGSN 100

Query: 173 LFN 165
           L+N
Sbjct: 101 LYN 103


>sp|Q9JHE3|ASAH2_MOUSE Neutral ceramidase OS=Mus musculus GN=Asah2
           PE=1 SV=1
          Length = 756

 Score = 30.0 bits (66), Expect = 4.9
 Identities = 11/16 (68%), Positives = 13/16 (81%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFG 50
           IPD   PG+YRIR+FG
Sbjct: 713 IPDTAYPGIYRIRYFG 728


>sp|Q9NR71|ASAH2_HUMAN Neutral ceramidase OS=Homo sapiens GN=ASAH2
           PE=1 SV=2
          Length = 780

 Score = 29.6 bits (65), Expect = 6.4
 Identities = 11/16 (68%), Positives = 13/16 (81%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFG 50
           IPD   PG+YRIR+FG
Sbjct: 737 IPDTAQPGIYRIRYFG 752


>sp|P0C7U2|ASA2C_HUMAN Putative neutral ceramidase C OS=Homo sapiens
           GN=ASAH2C PE=3 SV=1
          Length = 622

 Score = 29.6 bits (65), Expect = 6.4
 Identities = 11/16 (68%), Positives = 13/16 (81%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFG 50
           IPD   PG+YRIR+FG
Sbjct: 579 IPDTAQPGIYRIRYFG 594


>sp|P0C7U1|ASA2B_HUMAN Putative inactive neutral ceramidase B
           OS=Homo sapiens GN=ASAH2B PE=2 SV=1
          Length = 165

 Score = 29.6 bits (65), Expect = 6.4
 Identities = 11/16 (68%), Positives = 13/16 (81%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFG 50
           IPD   PG+YRIR+FG
Sbjct: 122 IPDTAQPGIYRIRYFG 137
tr_hit_id A9TGT4
Definition tr|A9TGT4|A9TGT4_PHYPA Predicted protein OS=Physcomitrella patens subsp. patens
Align length 37
Score (bit) 58.2
E-value 2.0e-07
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK960714|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0008_C14, 5'
         (455 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|A9TGT4|A9TGT4_PHYPA Predicted protein OS=Physcomitrella paten...    58   2e-07
tr|Q5ZE61|Q5ZE61_ORYSJ Neutral ceramidase-like OS=Oryza sativa s...    54   3e-06
tr|Q0JL46|Q0JL46_ORYSJ cDNA clone:J013054N18, full insert sequen...    54   3e-06
tr|A7NYM9|A7NYM9_VITVI Chromosome chr6 scaffold_3, whole genome ...    54   3e-06
tr|A2ZVJ7|A2ZVJ7_ORYSJ Putative uncharacterized protein OS=Oryza...    54   3e-06
tr|A2WSS4|A2WSS4_ORYSI Putative uncharacterized protein OS=Oryza...    54   3e-06
tr|A9PHI9|A9PHI9_POPTR Putative uncharacterized protein OS=Popul...    53   9e-06
tr|A7PNZ3|A7PNZ3_VITVI Chromosome chr8 scaffold_23, whole genome...    52   1e-05
tr|A5C3V2|A5C3V2_VITVI Putative uncharacterized protein OS=Vitis...    52   1e-05
tr|Q9FIL4|Q9FIL4_ARATH Neutral ceramidase OS=Arabidopsis thalian...    49   1e-04
tr|Q93ZI6|Q93ZI6_ARATH AT5g58980/k19m22_180 OS=Arabidopsis thali...    49   1e-04
tr|B7ZZK1|B7ZZK1_MAIZE Putative uncharacterized protein OS=Zea m...    49   2e-04
tr|Q5C3C4|Q5C3C4_SCHJA SJCHGC05525 protein (Fragment) OS=Schisto...    48   2e-04
tr|B5TWK7|B5TWK7_HORVU Neutral ceramidase OS=Hordeum vulgare PE=...    48   3e-04
tr|A9YFM2|A9YFM2_WHEAT Neutral ceramidase OS=Triticum aestivum P...    47   6e-04
tr|A9RDV4|A9RDV4_PHYPA Predicted protein OS=Physcomitrella paten...    44   0.004
tr|A4FEG6|A4FEG6_SACEN Possible hydrolase OS=Saccharopolyspora e...    42   0.015
tr|Q7QG18|Q7QG18_ANOGA AGAP003730-PA OS=Anopheles gambiae GN=AGA...    42   0.020
tr|Q5TXH8|Q5TXH8_ANOGA AGAP012841-PA OS=Anopheles gambiae str. P...    42   0.020
tr|A1ZDK8|A1ZDK8_9SPHI Alkaline ceramidase OS=Microscilla marina...    38   0.22 
tr|B6NKP8|B6NKP8_BRAFL Putative uncharacterized protein OS=Branc...    38   0.22 
tr|Q9LNV7|Q9LNV7_ARATH F22G5.28 OS=Arabidopsis thaliana PE=4 SV=1      38   0.29 
tr|Q173S2|Q173S2_AEDAE Ceramidase OS=Aedes aegypti GN=AAEL007030...    38   0.29 
tr|B0WGE6|B0WGE6_CULQU Ceramidase OS=Culex quinquefasciatus GN=C...    38   0.29 
tr|B6NKQ4|B6NKQ4_BRAFL Putative uncharacterized protein OS=Branc...    37   0.64 
tr|A4RHG8|A4RHG8_MAGGR Putative uncharacterized protein OS=Magna...    37   0.64 
tr|B2HSJ6|B2HSJ6_MYCMM Ceramidase OS=Mycobacterium marinum (stra...    36   0.84 
tr|B7QN43|B7QN43_IXOSC Ceramidase, putative (Fragment) OS=Ixodes...    36   0.84 
tr|B1VLK8|B1VLK8_STRGG Putative uncharacterized protein OS=Strep...    36   1.1  
tr|Q7QI06|Q7QI06_ANOGA AGAP000973-PA OS=Anopheles gambiae GN=AGA...    36   1.1  

>tr|A9TGT4|A9TGT4_PHYPA Predicted protein OS=Physcomitrella patens
           subsp. patens GN=PHYPADRAFT_92578 PE=4 SV=1
          Length = 741

 Score = 58.2 bits (139), Expect = 2e-07
 Identities = 24/37 (64%), Positives = 29/37 (78%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFGAAKHFFGSVRHFIGTTKSFVV 113
           +P   +PGVYRIRHFGA KHF GSV+HF G + +FVV
Sbjct: 703 VPKTSIPGVYRIRHFGAYKHFLGSVKHFTGASSAFVV 739


>tr|Q5ZE61|Q5ZE61_ORYSJ Neutral ceramidase-like OS=Oryza sativa
           subsp. japonica GN=P0501G01.30-1 PE=4 SV=1
          Length = 325

 Score = 54.3 bits (129), Expect = 3e-06
 Identities = 22/37 (59%), Positives = 29/37 (78%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFGAAKHFFGSVRHFIGTTKSFVV 113
           +P+    GVYR+RHFGA+K  FGSVRHF GT+++F V
Sbjct: 288 VPEDAAAGVYRLRHFGASKPMFGSVRHFTGTSRAFAV 324


>tr|Q0JL46|Q0JL46_ORYSJ cDNA clone:J013054N18, full insert sequence
           OS=Oryza sativa subsp. japonica GN=Os01g0624000 PE=2
           SV=1
          Length = 785

 Score = 54.3 bits (129), Expect = 3e-06
 Identities = 22/37 (59%), Positives = 29/37 (78%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFGAAKHFFGSVRHFIGTTKSFVV 113
           +P+    GVYR+RHFGA+K  FGSVRHF GT+++F V
Sbjct: 748 VPEDAAAGVYRLRHFGASKPMFGSVRHFTGTSRAFAV 784


>tr|A7NYM9|A7NYM9_VITVI Chromosome chr6 scaffold_3, whole genome
           shotgun sequence OS=Vitis vinifera GN=GSVIVT00024181001
           PE=4 SV=1
          Length = 773

 Score = 54.3 bits (129), Expect = 3e-06
 Identities = 23/37 (62%), Positives = 28/37 (75%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFGAAKHFFGSVRHFIGTTKSFVV 113
           IP+    GVYRIRHFGA+K  FGS+ HF GT+ +FVV
Sbjct: 736 IPESAAAGVYRIRHFGASKSLFGSISHFTGTSSAFVV 772


>tr|A2ZVJ7|A2ZVJ7_ORYSJ Putative uncharacterized protein OS=Oryza
           sativa subsp. japonica GN=OsJ_002569 PE=4 SV=1
          Length = 914

 Score = 54.3 bits (129), Expect = 3e-06
 Identities = 22/37 (59%), Positives = 29/37 (78%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFGAAKHFFGSVRHFIGTTKSFVV 113
           +P+    GVYR+RHFGA+K  FGSVRHF GT+++F V
Sbjct: 877 VPEDAAAGVYRLRHFGASKPMFGSVRHFTGTSRAFAV 913


>tr|A2WSS4|A2WSS4_ORYSI Putative uncharacterized protein OS=Oryza
           sativa subsp. indica GN=OsI_02918 PE=4 SV=1
          Length = 755

 Score = 54.3 bits (129), Expect = 3e-06
 Identities = 22/37 (59%), Positives = 29/37 (78%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFGAAKHFFGSVRHFIGTTKSFVV 113
           +P+    GVYR+RHFGA+K  FGSVRHF GT+++F V
Sbjct: 718 VPEDAAAGVYRLRHFGASKPMFGSVRHFTGTSRAFAV 754


>tr|A9PHI9|A9PHI9_POPTR Putative uncharacterized protein OS=Populus
           trichocarpa PE=2 SV=1
          Length = 268

 Score = 52.8 bits (125), Expect = 9e-06
 Identities = 23/37 (62%), Positives = 27/37 (72%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFGAAKHFFGSVRHFIGTTKSFVV 113
           IP    PGVYRIRHFGAAK   GS+ HF G++ +FVV
Sbjct: 231 IPQSASPGVYRIRHFGAAKGLLGSISHFTGSSSAFVV 267


>tr|A7PNZ3|A7PNZ3_VITVI Chromosome chr8 scaffold_23, whole genome
           shotgun sequence OS=Vitis vinifera GN=GSVIVT00021450001
           PE=4 SV=1
          Length = 766

 Score = 52.4 bits (124), Expect = 1e-05
 Identities = 20/37 (54%), Positives = 30/37 (81%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFGAAKHFFGSVRHFIGTTKSFVV 113
           +P+  V GVYR+ HFGA+K+ FGS+RHF G++ +F+V
Sbjct: 729 VPESAVLGVYRMTHFGASKNIFGSIRHFTGSSSAFIV 765


>tr|A5C3V2|A5C3V2_VITVI Putative uncharacterized protein OS=Vitis
           vinifera GN=VITISV_014337 PE=4 SV=1
          Length = 304

 Score = 52.4 bits (124), Expect = 1e-05
 Identities = 20/37 (54%), Positives = 30/37 (81%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFGAAKHFFGSVRHFIGTTKSFVV 113
           +P+  V GVYR+ HFGA+K+ FGS+RHF G++ +F+V
Sbjct: 267 VPESAVLGVYRMTHFGASKNIFGSIRHFTGSSSAFIV 303


>tr|Q9FIL4|Q9FIL4_ARATH Neutral ceramidase OS=Arabidopsis thaliana
           PE=4 SV=1
          Length = 705

 Score = 48.9 bits (115), Expect = 1e-04
 Identities = 21/38 (55%), Positives = 27/38 (71%)
 Frame = +3

Query: 3   IPDRVVPGVYRIRHFGAAKHFFGSVRHFIGTTKSFVVY 116
           IP+   PGVYRI HFG+AK    S+ HF G++ +FVVY
Sbjct: 668 IPETASPGVYRITHFGSAKTPISSIHHFSGSSSAFVVY 705