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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK960831
Clone id TST39A01NGRL0008_H12
Library
Length 584
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0008_H12. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID -
Sequence 
GTGATTCCCAACGGAAACATTTCAAATTGATTGCCGGCGTGCTTCTGCTTCGGGAAGGAG
CCCCTGTTTCATACTGGTTGCGAAGAAGATCCTTACTGAGAATAGTGATGTCCGGCGTGT
GCCTCTACAGTCACCGTTTCAGATTGGAGGCCGCCTTTTGAAGAATGGAGCTCTCACGAG
ATGAATTGCAAGCTGATTATACTTATGAGGAAGAAGTTCAGATTCCCAGTGATTCTGAAG
AAGACGATGCTCAATCTGAAGATCTTGATGCTACAATCAGATCTTTGCAAAGGCTTTCTT
TCTTATTGGACAAAGACAAGATTTCAAAACAAACCCTTGCAGAGGACCACAAGGGGATCT
CAAAAAAACCGGAGGTGATAGATGATTTTTTCCGGAATTTCTTAGTCAGATTGGGCTGCA
CAAAGACCTTGAATGTGTTTGAGACAGAGTGGTTTGAACTGAAACAGAGTGGCAAATTAT
TGGATAGTCAGTTGGGTGATCTTCCTGATGAGTACACTAGGAGTATGCATTTGGAAGAAG
TGTTACAGAGTTTACAACAACAACTTCTACAGGCAAACTATCTC
■■Homology search results ■■ -
sp_hit_id P93107
Definition sp|P93107|PF20_CHLRE Flagellar WD repeat-containing protein Pf20 OS=Chlamydomonas reinhardtii
Align length 90
Score (bit) 67.4
E-value 5.0e-11
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK960831|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0008_H12, 5'
         (584 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P93107|PF20_CHLRE Flagellar WD repeat-containing protein Pf20...    67   5e-11
sp|Q8N0X2|SPG16_HUMAN Sperm-associated antigen 16 protein OS=Hom...    64   5e-10
sp|Q8K450|SPG16_MOUSE Sperm-associated antigen 16 protein OS=Mus...    54   6e-07
sp|P40798|STC_DROME Protein shuttle craft OS=Drosophila melanoga...    33   1.0  
sp|O68988|CSMI_CHLTE Chlorosome protein I OS=Chlorobium tepidum ...    31   5.2  

>sp|P93107|PF20_CHLRE Flagellar WD repeat-containing protein Pf20
           OS=Chlamydomonas reinhardtii GN=PF20 PE=2 SV=1
          Length = 606

 Score = 67.4 bits (163), Expect = 5e-11
 Identities = 35/90 (38%), Positives = 53/90 (58%)
 Frame = +3

Query: 270 ATIRSLQRLSFLLDKDKISKQTLAEDHKGISKKPEVIDDFFRNFLVRLGCTKTLNVFETE 449
           A +R LQ+ +   +      +T       + KKPEVIDDF RNF +++G ++T   FE E
Sbjct: 39  AALRKLQQFTSKQEAATGPARTTEVKPGQVVKKPEVIDDFLRNFFIKMGLSRTCECFEAE 98

Query: 450 WFELKQSGKLLDSQLGDLPDEYTRSMHLEE 539
           W+ELK +G+L +S    +PD Y R+  LE+
Sbjct: 99  WYELKATGRLDNST--TVPDVYLRNAELED 126


>sp|Q8N0X2|SPG16_HUMAN Sperm-associated antigen 16 protein OS=Homo
           sapiens GN=SPAG16 PE=2 SV=2
          Length = 631

 Score = 63.9 bits (154), Expect = 5e-10
 Identities = 39/114 (34%), Positives = 60/114 (52%)
 Frame = +3

Query: 195 DYTYEEEVQIPXXXXXXXXXXXXXXATIRSLQRLSFLLDKDKISKQTLAEDHKGISKKPE 374
           DY YEE   IP                I+  Q  +   D + + ++T+      +   PE
Sbjct: 56  DYEYEE---IPDDNFSIPEGEEDLAKAIQMAQEQA--TDTEILERKTVLPSKHAV---PE 107

Query: 375 VIDDFFRNFLVRLGCTKTLNVFETEWFELKQSGKLLDSQLGDLPDEYTRSMHLE 536
           VI+DF  NFL+++G T+TL+ F++EW+EL Q G      +G++PD YT+ M LE
Sbjct: 108 VIEDFLCNFLIKMGMTRTLDCFQSEWYELIQKGVTELRTVGNVPDVYTQIMLLE 161


>sp|Q8K450|SPG16_MOUSE Sperm-associated antigen 16 protein OS=Mus
           musculus GN=Spag16 PE=1 SV=1
          Length = 639

 Score = 53.9 bits (128), Expect = 6e-07
 Identities = 25/55 (45%), Positives = 37/55 (67%)
 Frame = +3

Query: 372 EVIDDFFRNFLVRLGCTKTLNVFETEWFELKQSGKLLDSQLGDLPDEYTRSMHLE 536
           E I+DF  NFL+++G T+TL+ F+ EW+EL Q        LG++PD Y++ M LE
Sbjct: 101 EAIEDFLCNFLIKMGMTRTLDCFQAEWYELIQKKGSDFKGLGNVPDVYSQVMLLE 155


>sp|P40798|STC_DROME Protein shuttle craft OS=Drosophila
           melanogaster GN=stc PE=1 SV=2
          Length = 1106

 Score = 33.1 bits (74), Expect = 1.0
 Identities = 19/65 (29%), Positives = 28/65 (43%)
 Frame = -3

Query: 504 EDHPTDYPIICHSVSVQTTLSQTHSRSLCSPI*LRNSGKNHLSPPVFLRSPCGPLQGFVL 325
           +D P DY   C  +     +S+T     C  +  R  G +H    +    PC P Q  V+
Sbjct: 437 QDLPRDYLCFCGKLK-NPPVSRTELAHSCGEVCCRIEGCSHACTLLCHPGPCPPCQANVV 495

Query: 324 KSCLC 310
           +SC C
Sbjct: 496 RSCGC 500


>sp|O68988|CSMI_CHLTE Chlorosome protein I OS=Chlorobium tepidum
           GN=csmI PE=1 SV=1
          Length = 244

 Score = 30.8 bits (68), Expect = 5.2
 Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 4/43 (9%)
 Frame = +2

Query: 35  RRASASGRSPCFILVAK----KILTENSDVRRVPLQSPFQIGG 151
           R+ +A GR  C   +AK    K+L+   +VRR+   +PFQ+ G
Sbjct: 67  RQIAAGGRIACQATIAKEGTVKVLSRPEEVRRMVFSNPFQLIG 109
tr_hit_id B6PDY7
Definition tr|B6PDY7|B6PDY7_BRAFL Putative uncharacterized protein (Fragment) OS=Branchiostoma floridae
Align length 61
Score (bit) 68.9
E-value 2.0e-10
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK960831|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0008_H12, 5'
         (584 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|B6PDY7|B6PDY7_BRAFL Putative uncharacterized protein (Fragmen...    69   2e-10
tr|B6NCM5|B6NCM5_BRAFL Putative uncharacterized protein OS=Branc...    69   2e-10
tr|A8ITB4|A8ITB4_CHLRE WD-repeat protein of flagellum central pa...    67   6e-10
tr|A0C1P9|A0C1P9_PARTE Chromosome undetermined scaffold_142, who...    67   1e-09
tr|A7SR29|A7SR29_NEMVE Predicted protein OS=Nematostella vectens...    66   1e-09
tr|B3S8M3|B3S8M3_TRIAD Putative uncharacterized protein (Fragmen...    65   2e-09
tr|Q4R715|Q4R715_MACFA Testis cDNA, clone: QtsA-16582, similar t...    64   6e-09
tr|Q53SC3|Q53SC3_HUMAN Putative uncharacterized protein PF20 (Fr...    62   2e-08
tr|Q4G1A2|Q4G1A2_HUMAN SPAG16 protein OS=Homo sapiens GN=SPAG16 ...    62   2e-08
tr|A0CBI9|A0CBI9_PARTE Chromosome undetermined scaffold_164, who...    60   7e-08
tr|A9TCE4|A9TCE4_PHYPA PF20 central pair apparatus protein OS=Ph...    57   1e-06
tr|Q0VBE7|Q0VBE7_MOUSE Spag16 protein OS=Mus musculus GN=Spag16 ...    54   6e-06
tr|B7ZN47|B7ZN47_MOUSE Putative uncharacterized protein OS=Mus m...    54   6e-06
tr|A4H9F5|A4H9F5_LEIBR Putative uncharacterized protein OS=Leish...    50   1e-04
tr|Q4QDZ5|Q4QDZ5_LEIMA Putative uncharacterized protein OS=Leish...    46   0.001
tr|A4HXS2|A4HXS2_LEIIN Putative uncharacterized protein OS=Leish...    46   0.002
tr|Q388K3|Q388K3_9TRYP Putative uncharacterized protein OS=Trypa...    44   0.005
tr|O96661|O96661_9TRYP Pf20 homolog OS=Trypanosoma brucei GN=TWD...    44   0.005
tr|A9V2K4|A9V2K4_MONBE Predicted protein OS=Monosiga brevicollis...    44   0.005
tr|Q4E5G0|Q4E5G0_TRYCR Putative uncharacterized protein OS=Trypa...    44   0.007
tr|A2DAR4|A2DAR4_TRIVA Putative uncharacterized protein OS=Trich...    44   0.009
tr|Q4SMZ5|Q4SMZ5_TETNG Chromosome 6 SCAF14544, whole genome shot...    43   0.011
tr|A2EAC7|A2EAC7_TRIVA WD repeat protein, putative OS=Trichomona...    41   0.056
tr|A0DJC2|A0DJC2_PARTE Chromosome undetermined scaffold_53, whol...    40   0.12 
tr|A8BA42|A8BA42_GIALA Sperm-associated WD-repeat protein OS=Gia...    39   0.16 
tr|B6KFZ2|B6KFZ2_TOXGO Flagellar WD-repeat protein PF20, putativ...    39   0.21 

>tr|B6PDY7|B6PDY7_BRAFL Putative uncharacterized protein (Fragment)
           OS=Branchiostoma floridae GN=BRAFLDRAFT_248284 PE=4 SV=1
          Length = 615

 Score = 68.9 bits (167), Expect = 2e-10
 Identities = 27/61 (44%), Positives = 45/61 (73%)
 Frame = +3

Query: 357 ISKKPEVIDDFFRNFLVRLGCTKTLNVFETEWFELKQSGKLLDSQLGDLPDEYTRSMHLE 536
           + ++PEV+DDF RN+L+++G  KTL  F+TEW+EL+Q G L +  +  +PD Y+R+  L+
Sbjct: 59  VIERPEVVDDFVRNYLLKMGMRKTLECFQTEWYELQQKGLLKEEDVQTVPDVYSRNQQLD 118

Query: 537 E 539
           +
Sbjct: 119 D 119


>tr|B6NCM5|B6NCM5_BRAFL Putative uncharacterized protein
           OS=Branchiostoma floridae GN=BRAFLDRAFT_127060 PE=4 SV=1
          Length = 725

 Score = 68.9 bits (167), Expect = 2e-10
 Identities = 27/61 (44%), Positives = 45/61 (73%)
 Frame = +3

Query: 357 ISKKPEVIDDFFRNFLVRLGCTKTLNVFETEWFELKQSGKLLDSQLGDLPDEYTRSMHLE 536
           + ++PEV+DDF RN+L+++G  KTL  F+TEW+EL+Q G L +  +  +PD Y+R+  L+
Sbjct: 74  VIERPEVVDDFVRNYLLKMGMRKTLECFQTEWYELQQKGLLKEEDVQTVPDVYSRNQQLD 133

Query: 537 E 539
           +
Sbjct: 134 D 134


>tr|A8ITB4|A8ITB4_CHLRE WD-repeat protein of flagellum central pair
           OS=Chlamydomonas reinhardtii GN=PF20 PE=4 SV=1
          Length = 521

 Score = 67.4 bits (163), Expect = 6e-10
 Identities = 35/90 (38%), Positives = 53/90 (58%)
 Frame = +3

Query: 270 ATIRSLQRLSFLLDKDKISKQTLAEDHKGISKKPEVIDDFFRNFLVRLGCTKTLNVFETE 449
           A +R LQ+ +   +      +T       + KKPEVIDDF RNF +++G ++T   FE E
Sbjct: 39  AALRKLQQFTSKQEAATGPARTTEVKPGQVVKKPEVIDDFLRNFFIKMGLSRTCECFEAE 98

Query: 450 WFELKQSGKLLDSQLGDLPDEYTRSMHLEE 539
           W+ELK +G+L +S    +PD Y R+  LE+
Sbjct: 99  WYELKATGRLDNST--TVPDVYLRNAELED 126


>tr|A0C1P9|A0C1P9_PARTE Chromosome undetermined scaffold_142, whole
           genome shotgun sequence OS=Paramecium tetraurelia
           GN=GSPATT00034193001 PE=4 SV=1
          Length = 622

 Score = 66.6 bits (161), Expect = 1e-09
 Identities = 28/62 (45%), Positives = 42/62 (67%)
 Frame = +3

Query: 354 GISKKPEVIDDFFRNFLVRLGCTKTLNVFETEWFELKQSGKLLDSQLGDLPDEYTRSMHL 533
           G+ K+PEV+DDF RNFL++    ++L VF+ EW+EL Q GKL    +G + D Y ++  L
Sbjct: 74  GVIKRPEVVDDFIRNFLIKNQMNRSLEVFQQEWYELSQKGKLATDGMGQVTDVYVQNEKL 133

Query: 534 EE 539
           +E
Sbjct: 134 KE 135


>tr|A7SR29|A7SR29_NEMVE Predicted protein OS=Nematostella vectensis
           GN=v1g192584 PE=4 SV=1
          Length = 592

 Score = 66.2 bits (160), Expect = 1e-09
 Identities = 26/61 (42%), Positives = 45/61 (73%)
 Frame = +3

Query: 357 ISKKPEVIDDFFRNFLVRLGCTKTLNVFETEWFELKQSGKLLDSQLGDLPDEYTRSMHLE 536
           ++ +PEV++DF RNFLV++G  KTL+ F+TEW+EL Q G L + ++  +PD + ++  L+
Sbjct: 69  VTTRPEVVEDFVRNFLVKMGMNKTLDCFQTEWYELMQKGLLNEEEVRIVPDLFVKNQQLD 128

Query: 537 E 539
           +
Sbjct: 129 D 129


>tr|B3S8M3|B3S8M3_TRIAD Putative uncharacterized protein (Fragment)
           OS=Trichoplax adhaerens GN=TRIADDRAFT_31080 PE=4 SV=1
          Length = 528

 Score = 65.5 bits (158), Expect = 2e-09
 Identities = 28/57 (49%), Positives = 42/57 (73%)
 Frame = +3

Query: 369 PEVIDDFFRNFLVRLGCTKTLNVFETEWFELKQSGKLLDSQLGDLPDEYTRSMHLEE 539
           PE+IDDF RNF V++G T+TL+ F+TEW+EL Q G+L    +  +PD YT++  L++
Sbjct: 1   PELIDDFVRNFFVKMGLTRTLDCFQTEWYELMQKGQLNPEDVSVVPDIYTKNDLLDQ 57


>tr|Q4R715|Q4R715_MACFA Testis cDNA, clone: QtsA-16582, similar to
           human PF20 (PF20), OS=Macaca fascicularis PE=2 SV=1
          Length = 631

 Score = 63.9 bits (154), Expect = 6e-09
 Identities = 39/114 (34%), Positives = 60/114 (52%)
 Frame = +3

Query: 195 DYTYEEEVQIPXXXXXXXXXXXXXXATIRSLQRLSFLLDKDKISKQTLAEDHKGISKKPE 374
           DY YEE   IP                I+  Q  +   D + + ++T+      +   PE
Sbjct: 56  DYEYEE---IPDDNFSIPEGEEDLAKAIQMAQEQA--TDTEILERKTVLPSKPAV---PE 107

Query: 375 VIDDFFRNFLVRLGCTKTLNVFETEWFELKQSGKLLDSQLGDLPDEYTRSMHLE 536
           VI+DF  NFL+++G T+TL+ F++EW+EL Q G      +G++PD YT+ M LE
Sbjct: 108 VIEDFLCNFLIKMGMTRTLDCFQSEWYELIQKGVTELRTVGNVPDVYTQIMLLE 161


>tr|Q53SC3|Q53SC3_HUMAN Putative uncharacterized protein PF20
           (Fragment) OS=Homo sapiens GN=PF20 PE=2 SV=1
          Length = 221

 Score = 62.4 bits (150), Expect = 2e-08
 Identities = 28/56 (50%), Positives = 41/56 (73%)
 Frame = +3

Query: 369 PEVIDDFFRNFLVRLGCTKTLNVFETEWFELKQSGKLLDSQLGDLPDEYTRSMHLE 536
           PEVI+DF  NFL+++G T+TL+ F++EW+EL Q G      +G++PD YT+ M LE
Sbjct: 13  PEVIEDFLCNFLIKMGMTRTLDCFQSEWYELIQKGVTELRTVGNVPDVYTQIMLLE 68


>tr|Q4G1A2|Q4G1A2_HUMAN SPAG16 protein OS=Homo sapiens GN=SPAG16
           PE=2 SV=1
          Length = 571

 Score = 62.4 bits (150), Expect = 2e-08
 Identities = 30/76 (39%), Positives = 49/76 (64%)
 Frame = +3

Query: 309 DKDKISKQTLAEDHKGISKKPEVIDDFFRNFLVRLGCTKTLNVFETEWFELKQSGKLLDS 488
           D + + ++T+      +   PEVI+DF  NFL+++G T+TL+ F++EW+EL Q G     
Sbjct: 29  DTEILERKTVLHSKHAV---PEVIEDFLCNFLIKMGMTRTLDCFQSEWYELIQKGVTGLR 85

Query: 489 QLGDLPDEYTRSMHLE 536
            +G++PD YT+ M LE
Sbjct: 86  TVGNVPDVYTQIMLLE 101


>tr|A0CBI9|A0CBI9_PARTE Chromosome undetermined scaffold_164, whole
           genome shotgun sequence OS=Paramecium tetraurelia
           GN=GSPATT00036939001 PE=4 SV=1
          Length = 587

 Score = 60.5 bits (145), Expect = 7e-08
 Identities = 34/92 (36%), Positives = 50/92 (54%), Gaps = 13/92 (14%)
 Frame = +3

Query: 303 LLDKDKISKQTLAEDH-------KGISKKP------EVIDDFFRNFLVRLGCTKTLNVFE 443
           L D DKI      ED+       + ++K P      EV+DDF RNFL++    ++L VF+
Sbjct: 44  LNDLDKIINDVKKEDYIPKAQPNERVTKGPGWKDRLEVVDDFIRNFLIKNQMNRSLEVFQ 103

Query: 444 TEWFELKQSGKLLDSQLGDLPDEYTRSMHLEE 539
            EW+EL Q GKL    +G + D Y ++  L+E
Sbjct: 104 QEWYELSQKGKLATDGMGQVTDVYIQNEKLKE 135