DK961209
Clone id TST39A01NGRL0009_H22
Library
Length 648
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0009_H22. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
CGCGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACACACACACACACACACACA
CACACACACACACACACACACAGTGACCTTGCAGAGTAGCGCTTGCATGTTCAGCGTGCA
GGTATTGAGGACGAATCATGGACGTAATCAGGTCAACGGTACAACTAGGTCTGAAAGCTC
ATAGCAGCATAGCCGTCTCTGCCGAGGGACAATCGCATACGTACCTGCAGGTGCTACAGT
CCGCATTGGATATCTCCTCTGAGCTCAAGACAATCCTATCTCGTCATCAGCCCGCAAATA
TTTCTGCGGTGAGCAATGGCCCCTCAAGGAGCACTCTTGGAGCTCATGTGGAAGCCCCGC
CACGTGTAGGAATAATCGCCAAGCCCAGTGCAGAATTTGTAGCAGGGCTTTGGGCAACAT
GGCTTTGTCGAGGAATTGCAGTTCCATTGGCATTGAGCTATCCGGAAGCCGAACTCCTGC
ATGTGCTCACTGATGCGGATATTTCGGTTGTTCTTGGAACATCAGACTATGAATCACAGC
TTAAACAAGCCTCTCACAAAGCCTCATCTACCTTTTTCTTACTGCCTCAAGTGAAGGCTA
TCGATAATTTGAAGCTGGCCAACATGTATGAATTATCATCCCTTCATG
■■Homology search results ■■ -
sp_hit_id Q4G176
Definition sp|Q4G176|ACSF3_HUMAN Acyl-CoA synthetase family member 3, mitochondrial OS=Homo sapiens
Align length 138
Score (bit) 53.1
E-value 1.0e-06
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK961209|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0009_H22, 5'
(648 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|Q4G176|ACSF3_HUMAN Acyl-CoA synthetase family member 3, mitoc... 53 1e-06
sp|Q6GLK6|ACSF3_XENLA Acyl-CoA synthetase family member 3, mitoc... 50 8e-06
sp|Q58DN7|ACSF3_BOVIN Acyl-CoA synthetase family member 3, mitoc... 50 1e-05
sp|Q3URE1|ACSF3_MOUSE Acyl-CoA synthetase family member 3, mitoc... 47 1e-04
sp|Q9R9J0|MYCB_BACSU Mycosubtilin synthetase subunit B OS=Bacill... 46 2e-04
sp|Q9R9I9|MYCC_BACSU Mycosubtilin synthetase subunit C OS=Bacill... 42 0.003
sp|P40806|PKSJ_BACSU Putative polyketide synthase pksJ OS=Bacill... 40 0.008
sp|Q70LM5|LGRC_BREPA Linear gramicidin synthetase subunit C OS=B... 40 0.008
sp|P39845|PPSA_BACSU Plipastatin synthetase subunit A OS=Bacillu... 40 0.014
sp|O30409|TYCC_BREPA Tyrocidine synthetase 3 OS=Brevibacillus pa... 39 0.031
sp|O68008|BACC_BACLI Bacitracin synthetase 3 OS=Bacillus licheni... 39 0.031
sp|Q9R9J1|MYCA_BACSU Mycosubtilin synthetase subunit A OS=Bacill... 37 0.12
sp|P27742|ACVS_EMENI N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-... 37 0.12
sp|Q04747|SRFAB_BACSU Surfactin synthetase subunit 2 OS=Bacillus... 36 0.15
sp|P27206|SRFAA_BACSU Surfactin synthetase subunit 1 OS=Bacillus... 36 0.15
sp|P94459|PPSD_BACSU Plipastatin synthetase subunit D OS=Bacillu... 36 0.15
sp|O68006|BACA_BACLI Bacitracin synthetase 1 OS=Bacillus licheni... 36 0.15
sp|P09095|TYCA_BREPA Tyrocidine synthetase 1 OS=Brevibacillus pa... 36 0.20
sp|Q70LM6|LGRB_BREPA Linear gramicidin synthetase subunit B OS=B... 36 0.20
sp|P25464|ACVS_CEPAC N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-... 34 0.58
sp|Q70LM7|LGRA_BREPA Linear gramicidin synthetase subunit A OS=B... 34 0.75
sp|P26046|ACVS2_PENCH N-(5-amino-5-carboxypentanoyl)-L-cysteinyl... 33 1.3
sp|P19787|ACVS1_PENCH N-(5-amino-5-carboxypentanoyl)-L-cysteinyl... 33 1.3
sp|Q01886|HTS1_COCCA HC-toxin synthetase OS=Cochliobolus carbonu... 32 2.2
sp|P0C064|GRSB_BREBE Gramicidin S synthetase 2 OS=Brevibacillus ... 32 2.2
sp|P0C063|GRSB_ANEMI Gramicidin S synthetase 2 OS=Aneurinibacill... 32 2.2
sp|O30408|TYCB_BREPA Tyrocidine synthetase 2 OS=Brevibacillus pa... 32 2.9
sp|Q9ESJ4|SPN90_MOUSE SH3 adapter protein SPIN90 OS=Mus musculus... 32 3.7
sp|Q70LM4|LGRD_BREPA Linear gramicidin synthetase subunit D OS=B... 32 3.7
sp|P45794|LEP4_AERHY Type 4 prepilin-like proteins leader peptid... 32 3.7

>sp|Q4G176|ACSF3_HUMAN Acyl-CoA synthetase family member 3,
mitochondrial OS=Homo sapiens GN=ACSF3 PE=2 SV=2
Length = 576

Score = 53.1 bits (126), Expect = 1e-06
Identities = 39/138 (28%), Positives = 59/138 (42%)
Frame = +3

Query: 189 IAVSAEGQSHTYLQVLQSALDISSELKTILSRHQPANISAVSNGPSRSTLGAHVEAPPRV 368
IA+ + HTY ++ +L +S E+ + G R RV
Sbjct: 57 IALVDQHGRHTYRELYSRSLRLSQEI---------CRLCGCVGGDLREE---------RV 98

Query: 369 GIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVLTDADISVVLGTSDYESQLKQ 548
+ A +V WA+W+ G+AVPL +P A+L +V+ D+ SVVL + +Y L
Sbjct: 99 SFLCANDASYVVAQWASWMSGGVAVPLYRKHPAAQLEYVICDSQSSVVLASQEYLELLSP 158

Query: 549 ASHKASSTFFLLPQVKAI 602
K LLP AI
Sbjct: 159 VVRKLGVP--LLPLTPAI 174


>sp|Q6GLK6|ACSF3_XENLA Acyl-CoA synthetase family member 3,
mitochondrial OS=Xenopus laevis GN=acsf3 PE=2 SV=1
Length = 578

Score = 50.4 bits (119), Expect = 8e-06
Identities = 23/67 (34%), Positives = 37/67 (55%)
Frame = +3

Query: 342 AHVEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVLTDADISVVLGT 521
+H P RV + ++ +V WA W+ IAVPL S+P +EL +VL D+ ++V+
Sbjct: 91 SHNNTPERVSFLCPNNSSYVVCQWAVWMSGAIAVPLCKSHPPSELKYVLQDSQSALVVAE 150

Query: 522 SDYESQL 542
Y + L
Sbjct: 151 ESYTNVL 157


>sp|Q58DN7|ACSF3_BOVIN Acyl-CoA synthetase family member 3,
mitochondrial OS=Bos taurus GN=ACSF3 PE=2 SV=1
Length = 586

Score = 50.1 bits (118), Expect = 1e-05
Identities = 33/124 (26%), Positives = 52/124 (41%)
Frame = +3

Query: 216 HTYLQVLQSALDISSELKTILSRHQPANISAVSNGPSRSTLGAHVEAPPRVGIIAKPSAE 395
HTY + +L +S E+ + A ++G R RV ++
Sbjct: 67 HTYKDLYLRSLRLSREI---------CQLRACADGDLREE---------RVSLLCSNDVS 108

Query: 396 FVAGLWATWLCRGIAVPLALSYPEAELLHVLTDADISVVLGTSDYESQLKQASHKASSTF 575
FV WA W+ G+AVPL +P A+L + + D+ SVVL ++ L + K
Sbjct: 109 FVVAQWAAWMSGGVAVPLYRKHPRAQLEYFIQDSRSSVVLAGPEHVELLSPVAQKLGVPL 168

Query: 576 FLLP 587
LP
Sbjct: 169 LPLP 172


>sp|Q3URE1|ACSF3_MOUSE Acyl-CoA synthetase family member 3,
mitochondrial OS=Mus musculus GN=Acsf3 PE=2 SV=2
Length = 583

Score = 46.6 bits (109), Expect = 1e-04
Identities = 24/80 (30%), Positives = 41/80 (51%)
Frame = +3

Query: 363 RVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVLTDADISVVLGTSDYESQL 542
RV + +V WA+W+ G+AVPL +PEA+L + + D+ S+V+ +Y +L
Sbjct: 97 RVSFLCSNDVSYVVAQWASWMSGGVAVPLYWKHPEAQLEYFIQDSRSSLVVVGQEYLERL 156

Query: 543 KQASHKASSTFFLLPQVKAI 602
+ + LLP A+
Sbjct: 157 SPLAQRLGVP--LLPLTPAV 174


>sp|Q9R9J0|MYCB_BACSU Mycosubtilin synthetase subunit B OS=Bacillus
subtilis GN=mycB PE=3 SV=1
Length = 5369

Score = 45.8 bits (107), Expect = 2e-04
Identities = 26/77 (33%), Positives = 40/77 (51%)
Frame = +3

Query: 312 SNGPSRSTLGAHVEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVLT 491
SN +R+ V+A V I+A+ S E + + A W G VPL YPE L +VL
Sbjct: 298 SNRLARTLRNYGVQADTLVAIMAERSLEMIVSIMAIWKAGGAYVPLDPEYPEERLQYVLN 357

Query: 492 DADISVVLGTSDYESQL 542
DA+ V++ +++ L
Sbjct: 358 DANADVLVVQRHFKNSL 374



Score = 38.1 bits (87), Expect = 0.040
Identities = 21/77 (27%), Positives = 37/77 (48%)
Frame = +3

Query: 312 SNGPSRSTLGAHVEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVLT 491
+N +R+ V+A V I++ S E V G+ A G VP+ YP+ + ++L
Sbjct: 1808 ANRLARTLRARGVQADQPVATISRNSIELVTGILAILKAGGAYVPIDPEYPQDRIQYILE 1867

Query: 492 DADISVVLGTSDYESQL 542
D+ ++L D Q+
Sbjct: 1868 DSKAGIILMPRDVRQQI 1884


>sp|Q9R9I9|MYCC_BACSU Mycosubtilin synthetase subunit C OS=Bacillus
subtilis GN=mycC PE=3 SV=1
Length = 2609

Score = 42.0 bits (97), Expect = 0.003
Identities = 24/77 (31%), Positives = 40/77 (51%)
Frame = +3

Query: 285 HQPANISAVSNGPSRSTLGAHVEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYP 464
+Q N A + + G H + P VGI+A+ S E + GL+ G VP+ +YP
Sbjct: 289 YQELNEKANQIARTLQSEGVHPDQP--VGIMAERSLEMIVGLFGILKAGGAYVPIDPTYP 346

Query: 465 EAELLHVLTDADISVVL 515
E + ++L D+D ++L
Sbjct: 347 EERIRYILEDSDTKLLL 363



Score = 37.7 bits (86), Expect = 0.052
Identities = 22/77 (28%), Positives = 36/77 (46%)
Frame = +3

Query: 312 SNGPSRSTLGAHVEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVLT 491
+N +R+ V + II+ S E V G+ A G VP+ YPE + ++L
Sbjct: 1816 ANQLARTLRAKGVRSDRTAAIISDHSIELVVGILAVLKAGGAYVPIDPDYPEQRIQYILN 1875

Query: 492 DADISVVLGTSDYESQL 542
D+ +VL S + +L
Sbjct: 1876 DSKTEIVLTQSHLQQRL 1892


>sp|P40806|PKSJ_BACSU Putative polyketide synthase pksJ OS=Bacillus
subtilis GN=pksJ PE=3 SV=2
Length = 5045

Score = 40.4 bits (93), Expect = 0.008
Identities = 35/128 (27%), Positives = 54/128 (42%), Gaps = 1/128 (0%)
Frame = +3

Query: 162 QLGLKAHSSIAVSAEGQSHTYLQVLQSALDISSELKTILSRHQPANISAVSNGPSRSTLG 341
Q K AVS EGQ+ TY ++ + + ++ L+
Sbjct: 1164 QQAKKTPDRAAVSYEGQTLTYRELDEKSTQLAIYLQ------------------------ 1199

Query: 342 AHVEAPPRV-GIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVLTDADISVVLG 518
AH P R+ GI S + + GL A G VPL SYP L ++L D+++ + L
Sbjct: 1200 AHGVGPDRLAGIYVDRSLDMLVGLLAILKAGGAYVPLDPSYPAERLEYMLEDSEVFITLT 1259

Query: 519 TSDYESQL 542
TS+ + L
Sbjct: 1260 TSELVNTL 1267


>sp|Q70LM5|LGRC_BREPA Linear gramicidin synthetase subunit C
OS=Brevibacillus parabrevis GN=lgrC PE=3 SV=1
Length = 7756

Score = 40.4 bits (93), Expect = 0.008
Identities = 24/66 (36%), Positives = 33/66 (50%)
Frame = +3

Query: 348 VEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVLTDADISVVLGTSD 527
VEA VG+ S + + GL A G VPL +YPE L +L DA++S++L
Sbjct: 1581 VEAGTLVGLCVDRSLDMLVGLLAILKAGGAYVPLDPAYPEERLAFMLADANVSILLTQKH 1640

Query: 528 YESQLK 545
Q K
Sbjct: 1641 LGKQWK 1646



Score = 38.9 bits (89), Expect = 0.023
Identities = 24/66 (36%), Positives = 32/66 (48%)
Frame = +3

Query: 348 VEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVLTDADISVVLGTSD 527
VEA VG+ S + + GL A G VP+ +YPE L +L DA IS++L
Sbjct: 515 VEAGTLVGLCVDRSLDMLIGLLAILKAGGAYVPIDPAYPEERLAFMLADAKISILLTQKH 574

Query: 528 YESQLK 545
Q K
Sbjct: 575 LGKQWK 580



Score = 32.3 bits (72), Expect = 2.2
Identities = 27/122 (22%), Positives = 53/122 (43%), Gaps = 2/122 (1%)
Frame = +3

Query: 192 AVSAEGQSHTYLQVLQSALDISSELKTILSRHQP--ANISAVSNGPSRSTLGAHVEAPPR 365
+V +E + + QV + A + ++L + Q A + A +N + + +
Sbjct: 4105 SVPSERDTCVHEQVARIAQQLPNQLAVVSDEGQITYAELDAKANQVANYLHKQGIISETL 4164

Query: 366 VGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVLTDADISVVLGTSDYESQLK 545
VG+ S + + G VP+ +YP+ L ++ DA++ VVL +QL
Sbjct: 4165 VGVCLDRSIDMLVAQLGILKAGGAYVPMDPAYPQERLAFMMQDAEMPVVLTQEHLLAQLP 4224

Query: 546 QA 551
+A
Sbjct: 4225 EA 4226



Score = 32.3 bits (72), Expect = 2.2
Identities = 25/110 (22%), Positives = 47/110 (42%)
Frame = +3

Query: 294 ANISAVSNGPSRSTLGAHVEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAE 473
A ++ +N + L V+ VGI + S E + VPL ++P+
Sbjct: 5671 AELNERANQLAHRLLAEGVKPDELVGICVERSPEMIVAFLGVMKAGAAYVPLDPAHPQER 5730

Query: 474 LLHVLTDADISVVLGTSDYESQLKQASHKASSTFFLLPQVKAIDNLKLAN 623
+ +++ D+ SV+L + +L +S QV +D+ +LAN
Sbjct: 5731 IAYMIEDSQASVLLTQASLTDRLPASSR----------QVICLDSDELAN 5770



Score = 31.2 bits (69), Expect = 4.9
Identities = 16/74 (21%), Positives = 36/74 (48%)
Frame = +3

Query: 294 ANISAVSNGPSRSTLGAHVEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAE 473
A+++ +N + L V+ VGI + S E + G++ VP+ + P+
Sbjct: 3092 ADLNQRANQLAHRLLAQEVKPDTLVGICVERSPEMIIGIFGVMKAGAAYVPIDPALPQER 3151

Query: 474 LLHVLTDADISVVL 515
+ +++ D+ S++L
Sbjct: 3152 IAYMVEDSQASILL 3165


>sp|P39845|PPSA_BACSU Plipastatin synthetase subunit A OS=Bacillus
subtilis GN=ppsA PE=1 SV=1
Length = 2561

Score = 39.7 bits (91), Expect = 0.014
Identities = 18/74 (24%), Positives = 37/74 (50%)
Frame = +3

Query: 360 PRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVLTDADISVVLGTSDYESQ 539
P V ++AK S + + G+ A G+ +P+ YP+A + ++L D+ ++L + +
Sbjct: 1548 PTVAVLAKRSIDAIVGVLAVMKAGGVYIPIDAHYPKARIEYILRDSGADILLLQRELKHL 1607

Query: 540 LKQASHKASSTFFL 581
+ + S FL
Sbjct: 1608 ISNSPESEMSHIFL 1621



Score = 30.4 bits (67), Expect = 8.3
Identities = 19/67 (28%), Positives = 35/67 (52%), Gaps = 5/67 (7%)
Frame = +3

Query: 330 STLGAHVEA-----PPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVLTD 494
S L AH+ A VG++++ S + + + A G +PL +YP+ L ++L D
Sbjct: 494 SRLAAHLAARGVTNESIVGVLSERSPDMLIAVLAVLKAGGAYLPLDPAYPKERLSYMLKD 553

Query: 495 ADISVVL 515
+ S++L
Sbjct: 554 SGASLLL 560


>sp|O30409|TYCC_BREPA Tyrocidine synthetase 3 OS=Brevibacillus
parabrevis GN=tycC PE=1 SV=1
Length = 6486

Score = 38.5 bits (88), Expect = 0.031
Identities = 21/56 (37%), Positives = 29/56 (51%)
Frame = +3

Query: 348 VEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVLTDADISVVL 515
V+ VGI+A S E V G+ A G VP+ YPE + ++LTD +VL
Sbjct: 2584 VQPDQPVGIMANRSVEMVVGILAILKAGGAYVPIDPEYPEERVAYMLTDCQARLVL 2639



Score = 35.4 bits (80), Expect = 0.26
Identities = 29/115 (25%), Positives = 52/115 (45%), Gaps = 3/115 (2%)
Frame = +3

Query: 219 TYLQVLQSALDISSELKTILSRHQPANISAVSNGPSR--STLGAHVEAPPR-VGIIAKPS 389
T Q+ + +D + + +L Q S ++ +R L A P R V I+A+ S
Sbjct: 5657 TLHQLFEEQVDKTPDQAALLFSEQSLTYSELNERANRLARVLRAKGVGPDRLVAIMAERS 5716

Query: 390 AEFVAGLWATWLCRGIAVPLALSYPEAELLHVLTDADISVVLGTSDYESQLKQAS 554
E V G+ G VP+ YP+ + ++L D++ +++L + L Q S
Sbjct: 5717 PEMVIGILGILKAGGAYVPVDPGYPQERIQYLLEDSNAALLLSQAHLLPLLAQVS 5771


tr_hit_id B8LL75
Definition tr|B8LL75|B8LL75_PICSI Putative uncharacterized protein OS=Picea sitchensis
Align length 165
Score (bit) 132.0
E-value 2.0e-29
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK961209|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0009_H22, 5'
(648 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|B8LL75|B8LL75_PICSI Putative uncharacterized protein OS=Picea... 132 2e-29
tr|Q94DS1|Q94DS1_ORYSJ P0460E08.27 protein OS=Oryza sativa subsp... 115 2e-24
tr|B8A9T7|B8A9T7_ORYSI Putative uncharacterized protein OS=Oryza... 113 9e-24
tr|A9T6P4|A9T6P4_PHYPA Predicted protein OS=Physcomitrella paten... 112 1e-23
tr|Q9LW70|Q9LW70_ARATH Long-chain-fatty-acid-CoA ligase-like pro... 110 7e-23
tr|Q94A48|Q94A48_ARATH AT3g16170/MSL1_21 OS=Arabidopsis thaliana... 107 5e-22
tr|Q8LFU5|Q8LFU5_ARATH Putative long-chain acyl-CoA synthetase O... 107 5e-22
tr|Q8H151|Q8H151_ARATH Putative long-chain acyl-CoA synthetase (... 107 5e-22
tr|B6T7E3|B6T7E3_MAIZE Long-chain-fatty-acid-CoA ligase-like pro... 104 4e-21
tr|A5C8F0|A5C8F0_VITVI Putative uncharacterized protein OS=Vitis... 101 4e-20
tr|A9RZN2|A9RZN2_PHYPA Predicted protein OS=Physcomitrella paten... 96 2e-18
tr|A2WVB5|A2WVB5_ORYSI Putative uncharacterized protein OS=Oryza... 92 2e-17
tr|A2ZY22|A2ZY22_ORYSJ Putative uncharacterized protein OS=Oryza... 92 3e-17
tr|A7PXA6|A7PXA6_VITVI Chromosome chr12 scaffold_36, whole genom... 78 4e-13
tr|A5B087|A5B087_VITVI Putative uncharacterized protein OS=Vitis... 78 4e-13
tr|Q5JMF7|Q5JMF7_ORYSJ Putative uncharacterized protein P0512C01... 75 4e-12
tr|A7P789|A7P789_VITVI Chromosome chr9 scaffold_7, whole genome ... 64 8e-09
tr|B2IWU7|B2IWU7_NOSP7 AMP-dependent synthetase and ligase OS=No... 57 1e-06
tr|Q4SI26|Q4SI26_TETNG Chromosome 5 SCAF14581, whole genome shot... 56 2e-06
tr|B6LQZ6|B6LQZ6_BRAFL Putative uncharacterized protein OS=Branc... 56 2e-06
tr|B4C510|B4C510_9CHRO AMP-dependent synthetase and ligase OS=Cy... 55 3e-06
tr|B6LR12|B6LR12_BRAFL Putative uncharacterized protein (Fragmen... 55 5e-06
tr|A1ZXX4|A1ZXX4_9SPHI Acyl-activating enzyme 13 OS=Microscilla ... 54 6e-06
tr|Q7NLK1|Q7NLK1_GLOVI Glr1122 protein OS=Gloeobacter violaceus ... 54 8e-06
tr|A8K4J8|A8K4J8_HUMAN cDNA FLJ77772 OS=Homo sapiens GN=LOC19732... 53 1e-05
tr|B7K021|B7K021_SYNP8 AMP-dependent synthetase and ligase OS=Sy... 52 2e-05
tr|A2QRV7|A2QRV7_ASPNC Contig An08c0170, complete genome. OS=Asp... 52 3e-05
tr|Q1D6A1|Q1D6A1_MYXXD Non-ribosomal peptide synthase/polyketide... 50 9e-05
tr|A3HS47|A3HS47_9SPHI Probable long chain fatty acid CoA ligase... 50 2e-04
tr|B7QLH9|B7QLH9_IXOSC Acyl-CoA synthetase, putative OS=Ixodes s... 49 2e-04

>tr|B8LL75|B8LL75_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 582

Score = 132 bits (331), Expect = 2e-29
Identities = 75/165 (45%), Positives = 105/165 (63%), Gaps = 13/165 (7%)
Frame = +3

Query: 138 MDVIRSTVQLGLKAHSSIAVSAEGQSHTYLQVLQSALDISSELKT----ILSRHQPANIS 305
M+V+R+ +LG +A AV+ +S++Y Q+ +SAL++S+ L+ S +PA
Sbjct: 1 MEVVRAAAKLGPEAQRKAAVTVAQKSYSYNQIFKSALELSNVLRAGHSLSASHGRPAEDD 60

Query: 306 AVSNGPSRSTLGAHVEAP---------PRVGIIAKPSAEFVAGLWATWLCRGIAVPLALS 458
+ + +H+E RVGIIAKPSAEFVAG+WATW+ G+AVPLALS
Sbjct: 61 CIED---TDVAVSHLEKTIRRSNNLDGARVGIIAKPSAEFVAGMWATWISGGVAVPLALS 117

Query: 459 YPEAELLHVLTDADISVVLGTSDYESQLKQASHKASSTFFLLPQV 593
YPEAELLHV+ DA +SVVLGT +Y L++ S+K S+ F LLP V
Sbjct: 118 YPEAELLHVMNDAGVSVVLGTEEYLELLEKISNKCSARFHLLPLV 162


>tr|Q94DS1|Q94DS1_ORYSJ P0460E08.27 protein OS=Oryza sativa subsp.
japonica GN=P0460E08.27 PE=3 SV=1
Length = 602

Score = 115 bits (288), Expect = 2e-24
Identities = 63/159 (39%), Positives = 99/159 (62%), Gaps = 4/159 (2%)
Frame = +3

Query: 138 MDVIRSTVQLGLKAHSSIAVSAEGQSHTYLQVLQSALDISSELKTILSRHQPANISAVSN 317
M+V++ ++ G A+ ++ +S+ +Q++ SALD+ + L+ P + S N
Sbjct: 204 MEVVQEVLKHGSTQGVHAAIRSDQKSYNLVQLIASALDVYNILRN--KNVPPLSFSMTQN 261

Query: 318 GPSRSTL----GAHVEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHV 485
G + S++ G R+GI+AKPS EFVAG++ TWL G+AVPLALSYPEAELLHV
Sbjct: 262 GSTGSSVKGINGTGFLGGARIGIVAKPSPEFVAGIFGTWLSGGVAVPLALSYPEAELLHV 321

Query: 486 LTDADISVVLGTSDYESQLKQASHKASSTFFLLPQVKAI 602
+ D+DIS++L T +++ ++ S K S+ LLP V +I
Sbjct: 322 MNDSDISLILSTKEHQDIMENISTKCSAHCSLLPSVTSI 360


>tr|B8A9T7|B8A9T7_ORYSI Putative uncharacterized protein OS=Oryza
sativa subsp. indica GN=OsI_03826 PE=4 SV=1
Length = 664

Score = 113 bits (283), Expect = 9e-24
Identities = 63/159 (39%), Positives = 98/159 (61%), Gaps = 4/159 (2%)
Frame = +3

Query: 138 MDVIRSTVQLGLKAHSSIAVSAEGQSHTYLQVLQSALDISSELKTILSRHQPANISAVSN 317
M+V++ ++ G A+ ++ +S+ +Q++ SALD+ + L P + S N
Sbjct: 78 MEVVQEVLKHGSTQGVRAAIRSDQKSYNLVQLIASALDVYNILCN--KNVPPLSFSMTQN 135

Query: 318 GPSRSTL----GAHVEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHV 485
G + S++ G R+GI+AKPS EFVAG++ TWL G+AVPLALSYPEAELLHV
Sbjct: 136 GSTGSSVKGINGTGFLGGARIGIVAKPSPEFVAGIFGTWLSGGVAVPLALSYPEAELLHV 195

Query: 486 LTDADISVVLGTSDYESQLKQASHKASSTFFLLPQVKAI 602
+ D+DIS++L T +++ ++ S K S+ LLP V +I
Sbjct: 196 MNDSDISLILSTKEHQDIMENISTKCSAHCSLLPSVTSI 234


>tr|A9T6P4|A9T6P4_PHYPA Predicted protein OS=Physcomitrella patens
subsp. patens GN=PHYPADRAFT_192148 PE=3 SV=1
Length = 554

Score = 112 bits (281), Expect = 1e-23
Identities = 64/156 (41%), Positives = 96/156 (61%), Gaps = 1/156 (0%)
Frame = +3

Query: 138 MDVIRSTVQL-GLKAHSSIAVSAEGQSHTYLQVLQSALDISSELKTILSRHQPANISAVS 314
M+V++ +L ++ ++IA+SA ++++Y +LQSA IS + H S S
Sbjct: 1 MEVLKLEKKLDSSQSQNAIAISAGQKNYSYGHLLQSAFQISK-----MMHHGEGVKSTKS 55

Query: 315 NGPSRSTLGAHVEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVLTD 494
NG + RVGI+AKPSAEFVA +WA WL +AVPLALSYPEAEL+++LTD
Sbjct: 56 NGTVVGSEDLIRLRGTRVGIMAKPSAEFVASMWAVWLNGAVAVPLALSYPEAELVYILTD 115

Query: 495 ADISVVLGTSDYESQLKQASHKASSTFFLLPQVKAI 602
AD+S V T ++ QL+ + K S+ + +LP+V +
Sbjct: 116 ADVSAVASTEEFRDQLEGVAKKCSADYLVLPEVSCV 151


>tr|Q9LW70|Q9LW70_ARATH Long-chain-fatty-acid-CoA ligase-like
protein OS=Arabidopsis thaliana PE=3 SV=1
Length = 608

Score = 110 bits (275), Expect = 7e-23
Identities = 62/155 (40%), Positives = 91/155 (58%)
Frame = +3

Query: 129 GRIMDVIRSTVQLGLKAHSSIAVSAEGQSHTYLQVLQSALDISSELKTILSRHQPANISA 308
G +M+V ++ + IA+ A+G+S++Y Q+ SAL IS + + N
Sbjct: 62 GSLMEVFKAAFSEASNSCDRIAIKADGKSYSYGQLTSSALRISK----LFLKDDTTN--- 114

Query: 309 VSNGPSRSTLGAHVEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVL 488
++ G R+GI+AKPSAEFVAG+ TW G+AVPLALSYPEAELLHV+
Sbjct: 115 -GGQETKKYEGFGSLKGARIGIVAKPSAEFVAGVLGTWFSGGVAVPLALSYPEAELLHVM 173

Query: 489 TDADISVVLGTSDYESQLKQASHKASSTFFLLPQV 593
D+DIS++L T D+ +K + K+ + F L+P V
Sbjct: 174 NDSDISLLLSTEDHSETMKTIAAKSGARFHLIPPV 208


>tr|Q94A48|Q94A48_ARATH AT3g16170/MSL1_21 OS=Arabidopsis thaliana
PE=2 SV=1
Length = 246

Score = 107 bits (268), Expect = 5e-22
Identities = 61/152 (40%), Positives = 89/152 (58%)
Frame = +3

Query: 138 MDVIRSTVQLGLKAHSSIAVSAEGQSHTYLQVLQSALDISSELKTILSRHQPANISAVSN 317
M+V ++ + IA+ A+G+S++Y Q+ SAL IS + + N
Sbjct: 1 MEVFKAAFSEASNSCDRIAIKADGKSYSYGQLTSSALRISK----LFLKDDTTN----GG 52

Query: 318 GPSRSTLGAHVEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVLTDA 497
++ G R+GI+AKPSAEFVAG+ TW G+AVPLALSYPEAELLHV+ D+
Sbjct: 53 QETKKYEGFGSLKGARIGIVAKPSAEFVAGVLGTWFSGGVAVPLALSYPEAELLHVMNDS 112

Query: 498 DISVVLGTSDYESQLKQASHKASSTFFLLPQV 593
DIS++L T D+ +K + K+ + F L+P V
Sbjct: 113 DISLLLSTEDHSETMKTIAAKSGARFHLIPPV 144


>tr|Q8LFU5|Q8LFU5_ARATH Putative long-chain acyl-CoA synthetase
OS=Arabidopsis thaliana PE=2 SV=1
Length = 544

Score = 107 bits (268), Expect = 5e-22
Identities = 61/152 (40%), Positives = 89/152 (58%)
Frame = +3

Query: 138 MDVIRSTVQLGLKAHSSIAVSAEGQSHTYLQVLQSALDISSELKTILSRHQPANISAVSN 317
M+V ++ + IA+ A+G+S++Y Q+ SAL IS + + N
Sbjct: 1 MEVFKAAFSEASNSCDRIAIKADGKSYSYGQLTSSALRISK----LFLKDDTTN----GG 52

Query: 318 GPSRSTLGAHVEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVLTDA 497
++ G R+GI+AKPSAEFVAG+ TW G+AVPLALSYPEAELLHV+ D+
Sbjct: 53 QETKKYEGFGSLKGARIGIVAKPSAEFVAGVLGTWFSGGVAVPLALSYPEAELLHVMNDS 112

Query: 498 DISVVLGTSDYESQLKQASHKASSTFFLLPQV 593
DIS++L T D+ +K + K+ + F L+P V
Sbjct: 113 DISLLLSTEDHSETMKTIAAKSGARFHLIPPV 144


>tr|Q8H151|Q8H151_ARATH Putative long-chain acyl-CoA synthetase
(Acyl-activating enzyme 13) OS=Arabidopsis thaliana
GN=At3g16170 PE=2 SV=1
Length = 544

Score = 107 bits (268), Expect = 5e-22
Identities = 61/152 (40%), Positives = 89/152 (58%)
Frame = +3

Query: 138 MDVIRSTVQLGLKAHSSIAVSAEGQSHTYLQVLQSALDISSELKTILSRHQPANISAVSN 317
M+V ++ + IA+ A+G+S++Y Q+ SAL IS + + N
Sbjct: 1 MEVFKAAFSEASNSCDRIAIKADGKSYSYGQLTSSALRISK----LFLKDDTTN----GG 52

Query: 318 GPSRSTLGAHVEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVLTDA 497
++ G R+GI+AKPSAEFVAG+ TW G+AVPLALSYPEAELLHV+ D+
Sbjct: 53 QETKKYEGFGSLKGARIGIVAKPSAEFVAGVLGTWFSGGVAVPLALSYPEAELLHVMNDS 112

Query: 498 DISVVLGTSDYESQLKQASHKASSTFFLLPQV 593
DIS++L T D+ +K + K+ + F L+P V
Sbjct: 113 DISLLLSTEDHSETMKTIAAKSGARFHLIPPV 144


>tr|B6T7E3|B6T7E3_MAIZE Long-chain-fatty-acid-CoA ligase-like
protein OS=Zea mays PE=2 SV=1
Length = 558

Score = 104 bits (260), Expect = 4e-21
Identities = 61/155 (39%), Positives = 95/155 (61%)
Frame = +3

Query: 138 MDVIRSTVQLGLKAHSSIAVSAEGQSHTYLQVLQSALDISSELKTILSRHQPANISAVSN 317
M+V++ ++ G + A +S++ +Q++ ++ D+ + L S++ N S+
Sbjct: 1 MEVVQEVLRHGSADSVRAVIRANKKSYSLVQLIAASFDVYNIL---CSKNMTPNGMQDSS 57

Query: 318 GPSRSTLGAHVEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAELLHVLTDA 497
R+ G A RVGI+AKPS EFVAG++ TWL G+AVPLALSYPEAELLHV+ D+
Sbjct: 58 SKGRNGTGFLHGA--RVGIVAKPSPEFVAGVFGTWLSGGVAVPLALSYPEAELLHVMNDS 115

Query: 498 DISVVLGTSDYESQLKQASHKASSTFFLLPQVKAI 602
DIS+VL T ++ ++ S K S+ +LP V +I
Sbjct: 116 DISMVLSTKEHHEIMENLSIKCSAYCSILPSVTSI 150


>tr|A5C8F0|A5C8F0_VITVI Putative uncharacterized protein OS=Vitis
vinifera GN=VITISV_010506 PE=3 SV=1
Length = 847

Score = 101 bits (251), Expect = 4e-20
Identities = 63/172 (36%), Positives = 92/172 (53%)
Frame = +3

Query: 114 ACRY*GRIMDVIRSTVQLGLKAHSSIAVSAEGQSHTYLQVLQSALDISSELKTILSRHQP 293
A RY M+VI++ + G S+A+ A +S++Y Q++ SA ISS L
Sbjct: 273 AARY-STFMEVIKAVARQGSATXESVAIRANQKSYSYNQLISSARKISSLL--------- 322

Query: 294 ANISAVSNGPSRSTLGAHVEAPPRVGIIAKPSAEFVAGLWATWLCRGIAVPLALSYPEAE 473
NG + L ++ S EFVAG+ TW G+AVPLALSYPEAE
Sbjct: 323 ------CNGDIKPELWLNL------------SVEFVAGILGTWFSGGVAVPLALSYPEAE 364

Query: 474 LLHVLTDADISVVLGTSDYESQLKQASHKASSTFFLLPQVKAIDNLKLANMY 629
LLHV+ D+D+S++L T DY ++ + K+S+ F L+P V +I + A Y
Sbjct: 365 LLHVMNDSDVSMILSTEDYRELMENVAAKSSAQFSLIPPVPSIPSPTSARDY 416