DK961413
Clone id TST39A01NGRL0010_A18
Library
Length 355
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0010_A18. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
CGAGGAATCACCTGCTAGACACAGCCTTGTTTATAGAGGGATGATCCCCATTCTTGGAGA
GGGTTCGGCAAGAGCTACTGACACAGAAGCTACTGATGCAATCCTTGGTGTGGCTTTGAA
GACTGCAATCTCTAAGAAGCTTTGCAAAGTTGGTGATGCAGTTGTTGCTATTCATCGAAT
TGGCATTGCCTCCGTTATCAAGATTATGGAAGTGAAATGAGGTTTGTGATGACGATGTTT
TGATGTTATCAGCAATCGTCTGGATTTTATCACCTGAATTTTCATTTTTGCAGGCCATCG
GTCATTGATTAAGCATGATTATTGATTAAGCACGAAAAAAAAAAAAAAAAAAAAA
■■Homology search results ■■ -
sp_hit_id P22200
Definition sp|P22200|KPYC_SOLTU Pyruvate kinase, cytosolic isozyme OS=Solanum tuberosum
Align length 72
Score (bit) 105.0
E-value 5.0e-23
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK961413|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0010_A18, 5'
(334 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|P22200|KPYC_SOLTU Pyruvate kinase, cytosolic isozyme OS=Solan... 105 5e-23
sp|Q42806|KPYC_SOYBN Pyruvate kinase, cytosolic isozyme OS=Glyci... 104 2e-22
sp|Q42954|KPYC_TOBAC Pyruvate kinase, cytosolic isozyme OS=Nicot... 90 4e-18
sp|O65595|KPYC_ARATH Probable pyruvate kinase, cytosolic isozyme... 78 2e-14
sp|P00548|KPYK_CHICK Pyruvate kinase muscle isozyme OS=Gallus ga... 30 4.9
sp|P11980|KPYM_RAT Pyruvate kinase isozymes M1/M2 OS=Rattus norv... 29 6.4
sp|Q5NVN0|KPYM_PONAB Pyruvate kinase isozyme M1 OS=Pongo abelii ... 29 6.4
sp|P14618|KPYM_HUMAN Pyruvate kinase isozymes M1/M2 OS=Homo sapi... 29 6.4
sp|Q875Z9|KPYK_SACCA Pyruvate kinase OS=Saccharomyces castellii ... 29 6.4
sp|P52480|KPYM_MOUSE Pyruvate kinase isozymes M1/M2 OS=Mus muscu... 29 8.4
sp|Q92122|KPYK_XENLA Pyruvate kinase muscle isozyme OS=Xenopus l... 29 8.4
sp|O44006|KPYK_EIMTE Pyruvate kinase OS=Eimeria tenella GN=PYK P... 29 8.4

>sp|P22200|KPYC_SOLTU Pyruvate kinase, cytosolic isozyme OS=Solanum
tuberosum PE=2 SV=1
Length = 510

Score = 105 bits (263), Expect = 5e-23
Identities = 51/72 (70%), Positives = 64/72 (88%)
Frame = +2

Query: 2 EESPARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAIHRI 181
+E+PARHSLVYRG+IP+LGEGSA+ATD+E+T+ IL ALK+A+++ LCK GDAVVA+HRI
Sbjct: 439 DETPARHSLVYRGLIPLLGEGSAKATDSESTEVILEAALKSAVTRGLCKPGDAVVALHRI 498

Query: 182 GIASVIKIMEVK 217
G ASVIKI VK
Sbjct: 499 GSASVIKICVVK 510


>sp|Q42806|KPYC_SOYBN Pyruvate kinase, cytosolic isozyme OS=Glycine
max PE=2 SV=1
Length = 511

Score = 104 bits (259), Expect = 2e-22
Identities = 51/72 (70%), Positives = 61/72 (84%)
Frame = +2

Query: 2 EESPARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAIHRI 181
+E+PARHSL+YRG+IPILGEGSA+ATD E+T+ IL ALK+A + LCK GDAVVA+HRI
Sbjct: 440 DETPARHSLIYRGLIPILGEGSAKATDAESTEVILEAALKSATERALCKPGDAVVALHRI 499

Query: 182 GIASVIKIMEVK 217
G ASVIKI VK
Sbjct: 500 GAASVIKICIVK 511


>sp|Q42954|KPYC_TOBAC Pyruvate kinase, cytosolic isozyme
OS=Nicotiana tabacum PE=2 SV=1
Length = 508

Score = 89.7 bits (221), Expect = 4e-18
Identities = 43/72 (59%), Positives = 58/72 (80%)
Frame = +2

Query: 2 EESPARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAIHRI 181
+ESPARHSL++RG++P+L GSARA+ E+T+ L AL+ A +K LCK GD+VVA+HR+
Sbjct: 437 DESPARHSLIFRGLVPVLHAGSARASHEESTEEALDFALQHAKTKGLCKQGDSVVALHRV 496

Query: 182 GIASVIKIMEVK 217
G ASVIKI+ VK
Sbjct: 497 GTASVIKIVTVK 508


>sp|O65595|KPYC_ARATH Probable pyruvate kinase, cytosolic isozyme
OS=Arabidopsis thaliana GN=At4g26390 PE=2 SV=1
Length = 497

Score = 77.8 bits (190), Expect = 2e-14
Identities = 39/71 (54%), Positives = 52/71 (73%)
Frame = +2

Query: 5 ESPARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAIHRIG 184
E+PARHSL+YRG++P+L GSARA+ E+T+ L A + K+LCK GD+VVA+ R G
Sbjct: 427 EAPARHSLIYRGLVPVLYAGSARASIDESTEETLEFASEYGKKKQLCKTGDSVVALFRTG 486

Query: 185 IASVIKIMEVK 217
A VIKI+ VK
Sbjct: 487 NAIVIKILTVK 497


>sp|P00548|KPYK_CHICK Pyruvate kinase muscle isozyme OS=Gallus
gallus GN=PKM2 PE=2 SV=2
Length = 530

Score = 29.6 bits (65), Expect = 4.9
Identities = 14/56 (25%), Positives = 27/56 (48%)
Frame = +2

Query: 5 ESPARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAI 172
+ AR + +YRG+ P+L + A E D + + + ++ K GD V+ +
Sbjct: 456 DQTARQAHLYRGVFPVLCKQPAHDAWAEDVDLRVNLGMNVGKARGFFKTGDLVIVL 511


>sp|P11980|KPYM_RAT Pyruvate kinase isozymes M1/M2 OS=Rattus
norvegicus GN=Pkm2 PE=1 SV=3
Length = 531

Score = 29.3 bits (64), Expect = 6.4
Identities = 14/53 (26%), Positives = 27/53 (50%)
Frame = +2

Query: 14 ARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAI 172
AR + +YRG+ P+L + + E D + +A+ ++ K GD V+ +
Sbjct: 460 ARQAHLYRGIFPVLCKDAVLDAWAEDVDLRVNLAMNVGKARGFFKKGDVVIVL 512


>sp|Q5NVN0|KPYM_PONAB Pyruvate kinase isozyme M1 OS=Pongo abelii
GN=PKM2 PE=2 SV=3
Length = 531

Score = 29.3 bits (64), Expect = 6.4
Identities = 14/53 (26%), Positives = 26/53 (49%)
Frame = +2

Query: 14 ARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAI 172
AR + +YRG+ P+L + + E D + A+ ++ K GD V+ +
Sbjct: 460 ARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVL 512


>sp|P14618|KPYM_HUMAN Pyruvate kinase isozymes M1/M2 OS=Homo sapiens
GN=PKM2 PE=1 SV=4
Length = 531

Score = 29.3 bits (64), Expect = 6.4
Identities = 14/53 (26%), Positives = 26/53 (49%)
Frame = +2

Query: 14 ARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAI 172
AR + +YRG+ P+L + + E D + A+ ++ K GD V+ +
Sbjct: 460 ARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVL 512


>sp|Q875Z9|KPYK_SACCA Pyruvate kinase OS=Saccharomyces castellii
GN=PYK1 PE=3 SV=1
Length = 501

Score = 29.3 bits (64), Expect = 6.4
Identities = 18/53 (33%), Positives = 27/53 (50%)
Frame = +2

Query: 14 ARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAI 172
AR S + RG+ P + E + A TE + L ++ AI + K GD V+I
Sbjct: 431 ARFSHLSRGVFPFVYEADSVADWTEDVELRLKFGIEKAIEMGVMKKGDTYVSI 483


>sp|P52480|KPYM_MOUSE Pyruvate kinase isozymes M1/M2 OS=Mus musculus
GN=Pkm2 PE=1 SV=4
Length = 531

Score = 28.9 bits (63), Expect = 8.4
Identities = 14/53 (26%), Positives = 27/53 (50%)
Frame = +2

Query: 14 ARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAI 172
AR + +YRG+ P+L + + E D + +A+ ++ K GD V+ +
Sbjct: 460 ARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVL 512


tr_hit_id B8LR82
Definition tr|B8LR82|B8LR82_PICSI Putative uncharacterized protein OS=Picea sitchensis
Align length 72
Score (bit) 109.0
E-value 6.0e-23
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK961413|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0010_A18, 5'
(334 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|B8LR82|B8LR82_PICSI Putative uncharacterized protein OS=Picea... 109 6e-23
tr|B8LL81|B8LL81_PICSI Putative uncharacterized protein OS=Picea... 109 6e-23
tr|A9SL78|A9SL78_PHYPA Pyruvate kinase OS=Physcomitrella patens ... 105 9e-22
tr|Q7XKB5|Q7XKB5_ORYSJ Pyruvate kinase OS=Oryza sativa subsp. ja... 105 1e-21
tr|Q259K4|Q259K4_ORYSA Pyruvate kinase OS=Oryza sativa GN=H0402C... 105 1e-21
tr|A2XYZ3|A2XYZ3_ORYSI Pyruvate kinase OS=Oryza sativa subsp. in... 105 1e-21
tr|B6TII5|B6TII5_MAIZE Pyruvate kinase, cytosolic isozyme OS=Zea... 105 1e-21
tr|Q3S1N4|Q3S1N4_SOLTU Pyruvate kinase OS=Solanum tuberosum GN=P... 104 2e-21
tr|Q9M511|Q9M511_LILLO Pyruvate kinase OS=Lilium longiflorum PE=... 103 3e-21
tr|Q8L7J5|Q8L7J5_SOYBN Pyruvate kinase OS=Glycine max PE=2 SV=1 103 3e-21
tr|Q8L7J4|Q8L7J4_SOYBN Pyruvate kinase OS=Glycine max PE=2 SV=1 103 3e-21
tr|Q4JIY4|Q4JIY4_CITSI Pyruvate kinase OS=Citrus sinensis PE=2 SV=1 103 6e-21
tr|Q1I0X5|Q1I0X5_CAPAN Pyruvate kinase OS=Capsicum annuum PE=2 SV=1 103 6e-21
tr|Q9FFP6|Q9FFP6_ARATH Pyruvate kinase OS=Arabidopsis thaliana G... 102 1e-20
tr|Q0WN17|Q0WN17_ARATH Pyruvate kinase OS=Arabidopsis thaliana G... 102 1e-20
tr|A9SLA6|A9SLA6_PHYPA Pyruvate kinase OS=Physcomitrella patens ... 102 1e-20
tr|A7QNP5|A7QNP5_VITVI Pyruvate kinase OS=Vitis vinifera GN=GSVI... 101 2e-20
tr|A9PFJ7|A9PFJ7_POPTR Pyruvate kinase OS=Populus trichocarpa PE... 100 3e-20
tr|Q9FNN1|Q9FNN1_ARATH Pyruvate kinase OS=Arabidopsis thaliana G... 100 5e-20
tr|A7Q9U0|A7Q9U0_VITVI Pyruvate kinase OS=Vitis vinifera GN=GSVI... 100 5e-20
tr|A9SVG1|A9SVG1_PHYPA Pyruvate kinase OS=Physcomitrella patens ... 97 5e-19
tr|A9TZX1|A9TZX1_PHYPA Pyruvate kinase OS=Physcomitrella patens ... 96 7e-19
tr|A9TB85|A9TB85_PHYPA Pyruvate kinase OS=Physcomitrella patens ... 94 3e-18
tr|A5BD69|A5BD69_VITVI Pyruvate kinase OS=Vitis vinifera GN=VITI... 90 6e-17
tr|B7FLG3|B7FLG3_MEDTR Putative uncharacterized protein OS=Medic... 88 2e-16
tr|A8IVR6|A8IVR6_CHLRE Pyruvate kinase OS=Chlamydomonas reinhard... 86 9e-16
tr|Q6IUP7|Q6IUP7_MAIZE Pyruvate kinase OS=Zea mays PE=2 SV=1 84 5e-15
tr|Q5F2M7|Q5F2M7_SOYBN Pyruvate kinase OS=Glycine max GN=pk3 PE=... 84 5e-15
tr|B6T574|B6T574_MAIZE Pyruvate kinase, cytosolic isozyme OS=Zea... 84 5e-15
tr|B7FLG2|B7FLG2_MEDTR Putative uncharacterized protein OS=Medic... 83 8e-15

>tr|B8LR82|B8LR82_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 510

Score = 109 bits (273), Expect = 6e-23
Identities = 51/72 (70%), Positives = 65/72 (90%)
Frame = +2

Query: 2 EESPARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAIHRI 181
+E+PARHSL+YRG+IP+L EGSA+ATD+E+T+ IL ALKTAI+K LCK GDA+VA+HRI
Sbjct: 439 DETPARHSLIYRGLIPLLAEGSAKATDSESTEVILDAALKTAIAKGLCKKGDAIVALHRI 498

Query: 182 GIASVIKIMEVK 217
G+ASVIKI +VK
Sbjct: 499 GVASVIKICDVK 510


>tr|B8LL81|B8LL81_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 510

Score = 109 bits (273), Expect = 6e-23
Identities = 51/72 (70%), Positives = 65/72 (90%)
Frame = +2

Query: 2 EESPARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAIHRI 181
+E+PARHSL+YRG+IP+L EGSA+ATD+E+T+ IL ALKTAI+K LCK GDA+VA+HRI
Sbjct: 439 DETPARHSLIYRGLIPLLAEGSAKATDSESTEVILDAALKTAIAKGLCKKGDAIVALHRI 498

Query: 182 GIASVIKIMEVK 217
G+ASVIKI +VK
Sbjct: 499 GVASVIKICDVK 510


>tr|A9SL78|A9SL78_PHYPA Pyruvate kinase OS=Physcomitrella patens
subsp. patens GN=PHYPADRAFT_131669 PE=3 SV=1
Length = 517

Score = 105 bits (263), Expect = 9e-22
Identities = 53/72 (73%), Positives = 63/72 (87%)
Frame = +2

Query: 2 EESPARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAIHRI 181
EESPARHSLV RG++ +L EGSA+ATD+E+TDAILG AL A+ +KLC VGD+VVAIHRI
Sbjct: 438 EESPARHSLVCRGLLSLLAEGSAKATDSESTDAILGAALDHALKRKLCIVGDSVVAIHRI 497

Query: 182 GIASVIKIMEVK 217
G ASVIKI+EVK
Sbjct: 498 GAASVIKIVEVK 509


>tr|Q7XKB5|Q7XKB5_ORYSJ Pyruvate kinase OS=Oryza sativa subsp.
japonica GN=OSJNBa0064G10.16 PE=2 SV=2
Length = 511

Score = 105 bits (262), Expect = 1e-21
Identities = 49/71 (69%), Positives = 62/71 (87%)
Frame = +2

Query: 5 ESPARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAIHRIG 184
E PARHSL+YRG++P+L EGSA+ATD+E+T+ IL ALK+A+ K+LCK GDAVVA+HRIG
Sbjct: 441 EGPARHSLIYRGLVPLLAEGSAKATDSESTEVILDAALKSAVQKQLCKPGDAVVALHRIG 500

Query: 185 IASVIKIMEVK 217
+ASVIKI VK
Sbjct: 501 VASVIKICIVK 511


>tr|Q259K4|Q259K4_ORYSA Pyruvate kinase OS=Oryza sativa
GN=H0402C08.9 PE=3 SV=1
Length = 511

Score = 105 bits (262), Expect = 1e-21
Identities = 49/71 (69%), Positives = 62/71 (87%)
Frame = +2

Query: 5 ESPARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAIHRIG 184
E PARHSL+YRG++P+L EGSA+ATD+E+T+ IL ALK+A+ K+LCK GDAVVA+HRIG
Sbjct: 441 EGPARHSLIYRGLVPLLAEGSAKATDSESTEVILDAALKSAVQKQLCKPGDAVVALHRIG 500

Query: 185 IASVIKIMEVK 217
+ASVIKI VK
Sbjct: 501 VASVIKICIVK 511


>tr|A2XYZ3|A2XYZ3_ORYSI Pyruvate kinase OS=Oryza sativa subsp.
indica GN=OsI_17926 PE=3 SV=1
Length = 511

Score = 105 bits (262), Expect = 1e-21
Identities = 49/71 (69%), Positives = 62/71 (87%)
Frame = +2

Query: 5 ESPARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAIHRIG 184
E PARHSL+YRG++P+L EGSA+ATD+E+T+ IL ALK+A+ K+LCK GDAVVA+HRIG
Sbjct: 441 EGPARHSLIYRGLVPLLAEGSAKATDSESTEVILDAALKSAVQKQLCKPGDAVVALHRIG 500

Query: 185 IASVIKIMEVK 217
+ASVIKI VK
Sbjct: 501 VASVIKICIVK 511


>tr|B6TII5|B6TII5_MAIZE Pyruvate kinase, cytosolic isozyme OS=Zea
mays PE=2 SV=1
Length = 513

Score = 105 bits (261), Expect = 1e-21
Identities = 49/71 (69%), Positives = 62/71 (87%)
Frame = +2

Query: 5 ESPARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAIHRIG 184
E PARHSL+YRG+IP+L EGSA+ATD+E+T+ IL ALK+A+ K+LCK GDA+VA+HRIG
Sbjct: 443 EGPARHSLIYRGLIPLLAEGSAKATDSESTEVILEAALKSAVQKQLCKPGDAIVALHRIG 502

Query: 185 IASVIKIMEVK 217
+ASVIKI VK
Sbjct: 503 VASVIKICIVK 513


>tr|Q3S1N4|Q3S1N4_SOLTU Pyruvate kinase OS=Solanum tuberosum GN=PKc
PE=3 SV=1
Length = 510

Score = 104 bits (260), Expect = 2e-21
Identities = 50/72 (69%), Positives = 64/72 (88%)
Frame = +2

Query: 2 EESPARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAIHRI 181
+E+PARHSLVYRG+IP+LGEGSA+ATD+E+T+ IL ALK+A+++ LCK GDAVVA+HRI
Sbjct: 439 DETPARHSLVYRGLIPLLGEGSAKATDSESTEVILEAALKSAVTRGLCKPGDAVVALHRI 498

Query: 182 GIASVIKIMEVK 217
G ASVIKI +K
Sbjct: 499 GSASVIKICVLK 510


>tr|Q9M511|Q9M511_LILLO Pyruvate kinase OS=Lilium longiflorum PE=2
SV=1
Length = 510

Score = 103 bits (258), Expect = 3e-21
Identities = 48/72 (66%), Positives = 63/72 (87%)
Frame = +2

Query: 2 EESPARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAIHRI 181
+ESPARHSL+YRG+IP+L EGSA+ATD E+T+ IL ALK+A +++LCK GD++VA+HRI
Sbjct: 439 DESPARHSLIYRGLIPLLAEGSAKATDAESTEVILEAALKSATARRLCKPGDSIVALHRI 498

Query: 182 GIASVIKIMEVK 217
G+ASVIKI VK
Sbjct: 499 GVASVIKICIVK 510


>tr|Q8L7J5|Q8L7J5_SOYBN Pyruvate kinase OS=Glycine max PE=2 SV=1
Length = 511

Score = 103 bits (258), Expect = 3e-21
Identities = 51/72 (70%), Positives = 61/72 (84%)
Frame = +2

Query: 2 EESPARHSLVYRGMIPILGEGSARATDTEATDAILGVALKTAISKKLCKVGDAVVAIHRI 181
+E+PARHSL+YRG+IPILGEGSA+ATD E+T+ IL ALK+A + LCK GDAVVA+HRI
Sbjct: 440 DETPARHSLIYRGLIPILGEGSAKATDAESTEVILEAALKSATQRGLCKPGDAVVALHRI 499

Query: 182 GIASVIKIMEVK 217
G ASVIKI VK
Sbjct: 500 GTASVIKICIVK 511