DK961524
Clone id TST39A01NGRL0010_F20
Library
Length 644
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0010_F20. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
GGACGGACCGTAAACCGAGTCACGGCTCTGCTTATCGCTTAGACTTGCGCAGGCAGAGCT
CTCTGCTGCTGCAAGTCTAGGCTAAGCTTAGCTGGGCTCTCCCCTCGCTCTTCCAGGAGC
CTGCTCTCTCGCCTTTCTCCGATCTTGTGCCCTGTCGCGGTTTTTCTTTGTGCACCATTC
TCAGGAATGGCTGCAATGGCAAAGTCTTGTCTTGACAAGACAACTGCTTTACAGGCCTCA
CCGTTTAGGCAGGATGGAGTTGCCTCCTCAGCGAAGTGTGGACAACATGCAATTCTAAGC
ATGCCCCTCCACCAGAGTAAAACCAGAAAGTTGAGACCTGCGAGTTTTGATAAGTTGCCA
ACACTAAGTTTGTTTCAACGCCAACGCTGTTTTGCCACATGGAACATGGAGGAATATATC
AGCAATTGCGGCTCCTATCGCACCGCCCCTGAGTGATCCTTCGTACAGAGCAAAGCTGGC
GGAAGAGTATGGCTTTTCTCAAATTGGTGAGCCCGTTCCTCCAAATGTGACACTGAAGAA
TGTGATGGACTCTTTGCCTCCNGAGGTGTTTGAGATTAACAACATGAAGGCATGGAGGAC
TGTAGCTATCACGGTTACTGCCTATGCAGCAGGGCTTTTTATGA
■■Homology search results ■■ -
sp_hit_id P48627
Definition sp|P48627|FAD6C_BRANA Omega-6 fatty acid desaturase, chloroplastic OS=Brassica napus
Align length 59
Score (bit) 85.1
E-value 3.0e-16
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK961524|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0010_F20, 5'
(644 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|P48627|FAD6C_BRANA Omega-6 fatty acid desaturase, chloroplast... 85 3e-16
sp|P48628|FAD6C_SOYBN Omega-6 fatty acid desaturase, chloroplast... 83 1e-15
sp|P48629|FAD6C_SPIOL Omega-6 fatty acid desaturase, chloroplast... 80 9e-15
sp|P46312|FAD6C_ARATH Omega-6 fatty acid desaturase, chloroplast... 80 1e-14
sp|P20388|DESA_SYNY3 Fatty acid desaturase OS=Synechocystis sp. ... 33 1.3
sp|Q84WP6|NAC43_ARATH NAC domain-containing protein 43 OS=Arabid... 31 6.3

>sp|P48627|FAD6C_BRANA Omega-6 fatty acid desaturase, chloroplastic
OS=Brassica napus PE=2 SV=1
Length = 443

Score = 85.1 bits (209), Expect = 3e-16
Identities = 38/59 (64%), Positives = 51/59 (86%)
Frame = +2

Query: 467 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 643
R +LAE YGF QIG+ +P NVTLK++MD+LP EVFEI+++KAW++V I+VT+YA GLFM
Sbjct: 80 REQLAESYGFKQIGQDLPDNVTLKDIMDTLPKEVFEIDDVKAWKSVLISVTSYALGLFM 138


>sp|P48628|FAD6C_SOYBN Omega-6 fatty acid desaturase, chloroplastic
OS=Glycine max PE=2 SV=1
Length = 424

Score = 83.2 bits (204), Expect = 1e-15
Identities = 37/60 (61%), Positives = 52/60 (86%)
Frame = +2

Query: 464 YRAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 643
YR +LAE+YGF Q+GEP+ +VTLK+V++ LP EVFEI+++KAW++V I+VT+YA GLFM
Sbjct: 78 YRKQLAEDYGFRQVGEPLSDDVTLKDVINPLPKEVFEIDDVKAWKSVLISVTSYALGLFM 137


>sp|P48629|FAD6C_SPIOL Omega-6 fatty acid desaturase, chloroplastic
OS=Spinacia oleracea GN=FAD6 PE=1 SV=1
Length = 447

Score = 80.1 bits (196), Expect = 9e-15
Identities = 35/57 (61%), Positives = 48/57 (84%)
Frame = +2

Query: 473 KLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 643
+LAE YGF QIGEP+P +VT+++++ SLP +VFEIN+ KAW TV I+VT+YA G+FM
Sbjct: 87 QLAERYGFQQIGEPLPDDVTMRDIITSLPKQVFEINDTKAWGTVLISVTSYALGIFM 143


>sp|P46312|FAD6C_ARATH Omega-6 fatty acid desaturase, chloroplastic
OS=Arabidopsis thaliana GN=FAD6 PE=1 SV=2
Length = 448

Score = 79.7 bits (195), Expect = 1e-14
Identities = 37/59 (62%), Positives = 49/59 (83%)
Frame = +2

Query: 467 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 643
R +LAE YGF QIGE +P NVTLK++MD+LP EVFEI+++KA ++V I+VT+Y GLFM
Sbjct: 85 REQLAESYGFRQIGEDLPENVTLKDIMDTLPKEVFEIDDLKALKSVLISVTSYTLGLFM 143


>sp|P20388|DESA_SYNY3 Fatty acid desaturase OS=Synechocystis sp.
(strain PCC 6803) GN=desA PE=3 SV=1
Length = 351

Score = 33.1 bits (74), Expect = 1.3
Identities = 15/37 (40%), Positives = 24/37 (64%)
Frame = +2

Query: 524 NVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAG 634
++ L++++ +LP E FE KAW +V IT+ A A G
Sbjct: 23 DLKLQDIIKTLPKECFEKKASKAWASVLITLGAIAVG 59


>sp|Q84WP6|NAC43_ARATH NAC domain-containing protein 43
OS=Arabidopsis thaliana GN=EMB2301 PE=2 SV=2
Length = 365

Score = 30.8 bits (68), Expect = 6.3
Identities = 19/63 (30%), Positives = 32/63 (50%), Gaps = 1/63 (1%)
Frame = -2

Query: 226 QLSCQDKTLPLQPFLRMVHKEKPRQ-GTRSEKGERAGSWKSEGRAQLSLA*TCSSRELCL 50
Q C+ T P + HK+K GTR+ + AG WK+ GR ++ + + R + +
Sbjct: 62 QEMCKIGTTPQNDWYFFSHKDKKYPTGTRTNRATAAGFWKATGRDKIIYS---NGRRIGM 118

Query: 49 RKS 41
RK+
Sbjct: 119 RKT 121


tr_hit_id A9TJT1
Definition tr|A9TJT1|A9TJT1_PHYPA Predicted protein OS=Physcomitrella patens subsp. patens
Align length 59
Score (bit) 91.7
E-value 4.0e-17
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK961524|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0010_F20, 5'
(644 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|A9TJT1|A9TJT1_PHYPA Predicted protein OS=Physcomitrella paten... 92 4e-17
tr|A9PGN3|A9PGN3_POPTR Putative uncharacterized protein OS=Popul... 89 2e-16
tr|A9NV95|A9NV95_PICSI Putative uncharacterized protein OS=Picea... 88 4e-16
tr|Q6DUV9|Q6DUV9_BRANA Omega-6 desaturase OS=Brassica napus PE=2... 85 3e-15
tr|Q6DUV4|Q6DUV4_BRANA Omega-6 desaturase OS=Brassica napus PE=4... 85 4e-15
tr|Q6DT48|Q6DT48_ARALP AT4G30950 (Fragment) OS=Arabidopsis lyrat... 85 4e-15
tr|Q09GN7|Q09GN7_DESSO Omega-6 fatty acid desaturase (Chloroplas... 84 6e-15
tr|A7XZU9|A7XZU9_9ROSI Chloroplast omega-6 fatty acid desaturase... 84 7e-15
tr|A9UKE1|A9UKE1_OLEEU Plastidial delta-12 oleate desaturase OS=... 84 1e-14
tr|Q6Z9G4|Q6Z9G4_ORYSJ Putative Omega-6 fatty acid desaturase, c... 82 2e-14
tr|B8BB37|B8BB37_ORYSI Putative uncharacterized protein OS=Oryza... 82 2e-14
tr|A3BTJ3|A3BTJ3_ORYSJ Putative uncharacterized protein OS=Oryza... 82 2e-14
tr|B6TGK8|B6TGK8_MAIZE Putative uncharacterized protein OS=Zea m... 81 5e-14
tr|Q5U9G0|Q5U9G0_OLEEU Chloroplast fatty acid desaturase 6 OS=Ol... 77 1e-12
tr|A3QQP5|A3QQP5_MESVI Chloroplast omega-6 fatty acid desaturase... 72 2e-11
tr|Q9AXY4|Q9AXY4_BRAOL Omega 6 reductase (Fragment) OS=Brassica ... 71 6e-11
tr|Q9AXY2|Q9AXY2_BRANA Omega 6 reductase (Fragment) OS=Brassica ... 65 5e-09
tr|Q9SBU4|Q9SBU4_CHLSW Chloroplast w6 desaturase OS=Chlamydomona... 63 2e-08
tr|Q9AXY3|Q9AXY3_BRACM Omega 6 reductase (Fragment) OS=Brassica ... 63 2e-08
tr|Q9AXY5|Q9AXY5_BRANA Omega 6 reductase (Fragment) OS=Brassica ... 62 3e-08
tr|Q9C600|Q9C600_ARATH Omega 6 reductase (Fragment) OS=Arabidops... 58 4e-07
tr|O48663|O48663_CHLRE Chloroplast w6 desaturase (Omega-6-FAD, c... 55 5e-06
tr|A7R2X6|A7R2X6_VITVI Chromosome undetermined scaffold_462, who... 53 2e-05
tr|A9SI95|A9SI95_PHYPA Predicted protein (Fragment) OS=Physcomit... 51 5e-05
tr|Q9ZSV8|Q9ZSV8_ACEAT Putative chloroplast desaturase (Fragment... 47 0.001
tr|A9SCR5|A9SCR5_PHYPA Predicted protein (Fragment) OS=Physcomit... 45 0.004
tr|Q55231|Q55231_SYNP2 Delta 12 desaturase OS=Synechococcus sp. ... 41 0.055
tr|Q0V6T8|Q0V6T8_PHANO Putative uncharacterized protein OS=Phaeo... 38 0.61
tr|A0ZMU5|A0ZMU5_NODSP Fatty acid desaturase OS=Nodularia spumig... 37 0.79
tr|B1WXI5|B1WXI5_CYAA5 Fatty acid desaturase OS=Cyanothece (stra... 37 1.0

>tr|A9TJT1|A9TJT1_PHYPA Predicted protein OS=Physcomitrella patens
subsp. patens GN=PHYPADRAFT_222981 PE=4 SV=1
Length = 454

Score = 91.7 bits (226), Expect = 4e-17
Identities = 41/59 (69%), Positives = 52/59 (88%)
Frame = +2

Query: 467 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 643
R +LAEEYGF+QIGEPVP +VTLK V+DS P EVFEIN++KAW+T+AI+V +Y+ GLFM
Sbjct: 92 RQRLAEEYGFTQIGEPVPADVTLKKVIDSFPSEVFEINDLKAWKTIAISVASYSLGLFM 150


>tr|A9PGN3|A9PGN3_POPTR Putative uncharacterized protein OS=Populus
trichocarpa PE=2 SV=1
Length = 444

Score = 89.0 bits (219), Expect = 2e-16
Identities = 39/60 (65%), Positives = 52/60 (86%)
Frame = +2

Query: 464 YRAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 643
YR +LAE YGF +IGEP+P N+TLK VMD+LP +VFEI++MKAW++V ++VT+YA GLFM
Sbjct: 81 YRKQLAESYGFRKIGEPLPDNITLKGVMDTLPKKVFEIDDMKAWKSVLVSVTSYAFGLFM 140


>tr|A9NV95|A9NV95_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 460

Score = 88.2 bits (217), Expect = 4e-16
Identities = 39/59 (66%), Positives = 52/59 (88%)
Frame = +2

Query: 467 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 643
R +++E+YGF+QIGEPVP N+ LKNV+DSLP EVFEI++ KAW+TVAI+V AY+ GLF+
Sbjct: 98 RNRISEKYGFTQIGEPVPDNIILKNVIDSLPQEVFEIDDTKAWKTVAISVAAYSLGLFL 156


>tr|Q6DUV9|Q6DUV9_BRANA Omega-6 desaturase OS=Brassica napus PE=2
SV=1
Length = 443

Score = 85.1 bits (209), Expect = 3e-15
Identities = 38/59 (64%), Positives = 51/59 (86%)
Frame = +2

Query: 467 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 643
R +LAE YGF QIG+ +P NVTLK++MD+LP EVFEI+++KAW++V I+VT+YA GLFM
Sbjct: 80 REQLAESYGFKQIGQDLPDNVTLKDIMDTLPKEVFEIDDVKAWKSVLISVTSYALGLFM 138


>tr|Q6DUV4|Q6DUV4_BRANA Omega-6 desaturase OS=Brassica napus PE=4
SV=1
Length = 443

Score = 84.7 bits (208), Expect = 4e-15
Identities = 38/59 (64%), Positives = 51/59 (86%)
Frame = +2

Query: 467 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 643
R +LAE YGF QIG+ +P NVTLK++MD+LP EVFEI+++KAW++V I+VT+YA GLFM
Sbjct: 80 REQLAESYGFKQIGQNLPDNVTLKDIMDTLPKEVFEIDDVKAWKSVLISVTSYALGLFM 138


>tr|Q6DT48|Q6DT48_ARALP AT4G30950 (Fragment) OS=Arabidopsis lyrata
subsp. petraea PE=4 SV=1
Length = 203

Score = 84.7 bits (208), Expect = 4e-15
Identities = 38/59 (64%), Positives = 50/59 (84%)
Frame = +2

Query: 467 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 643
R +LAE YGF QIGE +P NVTLK++MD+LP EVFEI+++KAW++V I+VT+Y GLFM
Sbjct: 50 REQLAESYGFKQIGENLPDNVTLKDIMDTLPKEVFEIDDLKAWKSVLISVTSYTLGLFM 108


>tr|Q09GN7|Q09GN7_DESSO Omega-6 fatty acid desaturase (Chloroplast
delta-12 fatty acid desaturase) OS=Descurainia sophia
GN=FAD6 PE=2 SV=1
Length = 447

Score = 84.3 bits (207), Expect = 6e-15
Identities = 38/59 (64%), Positives = 50/59 (84%)
Frame = +2

Query: 467 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 643
R +LAE YGF QIGE +P NVTLK++MD+LP EVFEI+++KAW++V I+VT+Y GLFM
Sbjct: 84 REQLAESYGFRQIGEDLPDNVTLKDIMDTLPKEVFEIDDVKAWKSVLISVTSYTLGLFM 142


>tr|A7XZU9|A7XZU9_9ROSI Chloroplast omega-6 fatty acid desaturase
OS=Jatropha curcas PE=2 SV=1
Length = 440

Score = 84.0 bits (206), Expect = 7e-15
Identities = 35/60 (58%), Positives = 51/60 (85%)
Frame = +2

Query: 464 YRAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 643
YR +LAE YGF QIGEP+P N+TL+ ++D+LP +VFEI+++KAW++V ++VT+YA GL M
Sbjct: 77 YRKQLAESYGFRQIGEPLPENITLREIIDTLPKKVFEIDDVKAWKSVLVSVTSYALGLLM 136


>tr|A9UKE1|A9UKE1_OLEEU Plastidial delta-12 oleate desaturase
OS=Olea europaea GN=FAD6 PE=2 SV=1
Length = 443

Score = 83.6 bits (205), Expect = 1e-14
Identities = 36/59 (61%), Positives = 52/59 (88%)
Frame = +2

Query: 467 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 643
R +L+E YGF QIGEP+P NVTLK+++D+LP +VFEI+++KAW++V I+VT+YA G+FM
Sbjct: 81 RKQLSENYGFRQIGEPLPDNVTLKDIVDTLPRKVFEIDDVKAWKSVLISVTSYALGIFM 139


>tr|Q6Z9G4|Q6Z9G4_ORYSJ Putative Omega-6 fatty acid desaturase,
chloroplast OS=Oryza sativa subsp. japonica
GN=P0528B09.19 PE=4 SV=1
Length = 414

Score = 82.4 bits (202), Expect = 2e-14
Identities = 36/59 (61%), Positives = 50/59 (84%)
Frame = +2

Query: 467 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 643
R ++ E+YGF QIGE +P NVTLK+VMDSLP EVFEI+++K+W +V I+VT+YA G+F+
Sbjct: 52 RKQMCEDYGFKQIGEQLPDNVTLKDVMDSLPKEVFEIDDLKSWTSVLISVTSYALGIFL 110