DK961695 |
Clone id |
TST39A01NGRL0010_N18 |
Library |
TST39 |
Length |
643 |
Definition |
Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0010_N18. 5' end sequence. |
Accession |
DK961695 |
Tissue type |
prothallia with plantlets |
Developmental stage |
gametophytes with sporophytes |
Contig ID |
CL142Contig1 |
Sequence |
TGGCTCTTGGCTGGTCAATTGCTCTTGGTTCACCTTTCACCTTTGCAACAACCTTGGAAG ATGAATACAGGAGTGACATTTATGGTGAAAGAGGTATTTTGCTTGGTGCTGTACATGGGC TGGTTGAGGCGCTGTTTAGACGATACACCGAGGCAGGTATGCCTGAAGAGGATGCCTACA AGAACACGGTAGAAGGCATTACAGGTGTTATATCAAAGACAATCTCCACAAAGGGAATGC TGGCTGTGTACAACTCTTTAGATGAGCAAGGGAAGAAAGATTTTGAGATTGCCTACAGTT CCTCATATTACCCCTGCATGGACATCCTTTATGAATGTTACGAGGATGTGGCTTCAGGAA ATGAGATTCTTTTTTTGTCCCCCCCCGGCCGCCGCTTTCAGGAAAAAGAAAAAAAACATG CTTTTCCGATGGGCAAGATTGACAAGACACGGATGTGGAAGGTGGGAGAGCGAGTGCGGG CTGTGCGGCCAGAGGGTGACCTTGGTCCTTTGTATGGGTTCACAGCTGGAGTTTACATTG CTCTCATGATGGCTCAAATTGAGGTCTTAAGGAAGAAAGGTCATTCTTACTCTGAGATGG TTAATGAGAGTGTCATCGAGGCTGTGGATTCATTGAATCCTTT |
■■Homology search results ■■ |
- |
Swiss-Prot (release 56.9) |
Link to BlastX Result : Swiss-Prot |
sp_hit_id |
Q01292 |
Definition |
sp|Q01292|ILV5_SPIOL Ketol-acid reductoisomerase, chloroplastic OS=Spinacia oleracea |
Align length |
213 |
Score (bit) |
356.0 |
E-value |
5.0e-98 |
Report |
BLASTX 2.2.19 [Nov-02-2008]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402.
Query= DK961695|Adiantum capillus-veneris mRNA, clone: TST39A01NGRL0010_N18, 5' (643 letters)
Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters
Searching..................................................done
Score E Sequences producing significant alignments: (bits) Value
sp|Q01292|ILV5_SPIOL Ketol-acid reductoisomerase, chloroplastic ... 356 5e-98 sp|O82043|ILV5_PEA Ketol-acid reductoisomerase, chloroplastic OS... 350 4e-96 sp|Q05758|ILV5_ARATH Ketol-acid reductoisomerase, chloroplastic ... 348 1e-95 sp|P06168|ILV5_YEAST Ketol-acid reductoisomerase, mitochondrial ... 80 7e-15 sp|P78827|ILV5_SCHPO Probable ketol-acid reductoisomerase, mitoc... 77 8e-14 sp|P38674|ILV5_NEUCR Ketol-acid reductoisomerase, mitochondrial ... 76 1e-13 sp|A6Q461|ILVC_NITSB Ketol-acid reductoisomerase OS=Nitratirupto... 49 2e-05 sp|Q5SJ03|ILVC_THET8 Ketol-acid reductoisomerase OS=Thermus ther... 49 3e-05 sp|Q72JC8|ILVC_THET2 Ketol-acid reductoisomerase OS=Thermus ther... 49 3e-05 sp|Q7M851|ILVC_WOLSU Ketol-acid reductoisomerase OS=Wolinella su... 48 4e-05 sp|Q7VGW6|ILVC_HELHP Ketol-acid reductoisomerase OS=Helicobacter... 48 4e-05 sp|A4SFC5|ILVC_PROVI Ketol-acid reductoisomerase OS=Prosthecochl... 45 2e-04 sp|A1BES5|ILVC_CHLPD Ketol-acid reductoisomerase OS=Chlorobium p... 45 2e-04 sp|A7I0D4|ILVC_CAMHC Ketol-acid reductoisomerase OS=Campylobacte... 45 3e-04 sp|Q2S0M9|ILVC_SALRD Ketol-acid reductoisomerase OS=Salinibacter... 45 4e-04 sp|Q30T61|ILVC_SULDN Ketol-acid reductoisomerase OS=Sulfurimonas... 44 5e-04 sp|Q3APC3|ILVC_CHLCH Ketol-acid reductoisomerase OS=Chlorobium c... 44 5e-04 sp|Q8DW43|ILVC_STRMU Ketol-acid reductoisomerase OS=Streptococcu... 44 7e-04 sp|Q3B594|ILVC_PELLD Ketol-acid reductoisomerase OS=Pelodictyon ... 44 7e-04 sp|Q8KER7|ILVC_CHLTE Ketol-acid reductoisomerase OS=Chlorobium t... 44 7e-04 sp|Q6LZH4|ILVC_METMP Ketol-acid reductoisomerase OS=Methanococcu... 44 0.001 sp|A6VJW0|ILVC_METM7 Ketol-acid reductoisomerase OS=Methanococcu... 44 0.001 sp|A4FYE4|ILVC_METM5 Ketol-acid reductoisomerase OS=Methanococcu... 44 0.001 sp|A8ERD8|ILVC_ARCB4 Ketol-acid reductoisomerase OS=Arcobacter b... 43 0.001 sp|P65149|ILVC_MYCTU Ketol-acid reductoisomerase OS=Mycobacteriu... 42 0.002 sp|A5U713|ILVC_MYCTA Ketol-acid reductoisomerase OS=Mycobacteriu... 42 0.002 sp|Q73VH7|ILVC_MYCPA Ketol-acid reductoisomerase OS=Mycobacteriu... 42 0.002 sp|O33114|ILVC_MYCLE Ketol-acid reductoisomerase OS=Mycobacteriu... 42 0.002 sp|A1KMZ6|ILVC_MYCBP Ketol-acid reductoisomerase OS=Mycobacteriu... 42 0.002 sp|P65150|ILVC_MYCBO Ketol-acid reductoisomerase OS=Mycobacteriu... 42 0.002
>sp|Q01292|ILV5_SPIOL Ketol-acid reductoisomerase, chloroplastic OS=Spinacia oleracea GN=AHRI PE=1 SV=1 Length = 595
Score = 356 bits (914), Expect = 5e-98 Identities = 176/213 (82%), Positives = 194/213 (91%) Frame = +3
Query: 3 ALGWSIALGSPFTFATTLEDEYRSDIYGERGILLGAVHGLVEALFRRYTEAGMPEEDAYK 182 ALGWSIALGSPFTFATTLE EY+SDI+GERGILLGAVHG+VE LFRRYTE+GM E+ AYK Sbjct: 291 ALGWSIALGSPFTFATTLEQEYKSDIFGERGILLGAVHGIVECLFRRYTESGMSEDLAYK 350
Query: 183 NTVEGITGVISKTISTKGMLAVYNSLDEQGKKDFEIAYSSSYYPCMDILYECYEDVASGN 362 NTVE ITGVISKTISTKGMLA+YNSL E+GKKDF+ AYS+SYYP MDILYECYEDVASG+ Sbjct: 351 NTVECITGVISKTISTKGMLALYNSLSEEGKKDFQAAYSASYYPSMDILYECYEDVASGS 410
Query: 363 EILFLSPPGRRFQEKEKKHAFPMGKIDKTRMWKVGERVRAVRPEGDLGPLYGFTAGVYIA 542 EI + GRRF EKE AFPMGKID+TRMWKVGE+VR+VRP GDLGPLY FTAGVY+A Sbjct: 411 EIRSVVLAGRRFYEKEGLPAFPMGKIDQTRMWKVGEKVRSVRPAGDLGPLYPFTAGVYVA 470
Query: 543 LMMAQIEVLRKKGHSYSEMVNESVIEAVDSLNP 641 LMMAQIE+LRKKGHSYSE++NESVIEAVDSLNP Sbjct: 471 LMMAQIEILRKKGHSYSEIINESVIEAVDSLNP 503
>sp|O82043|ILV5_PEA Ketol-acid reductoisomerase, chloroplastic OS=Pisum sativum GN=PGAAIR PE=2 SV=1 Length = 581
Score = 350 bits (898), Expect = 4e-96 Identities = 171/213 (80%), Positives = 193/213 (90%) Frame = +3
Query: 3 ALGWSIALGSPFTFATTLEDEYRSDIYGERGILLGAVHGLVEALFRRYTEAGMPEEDAYK 182 ALGWS+ALGSPFTFATTLE EY+SDI+GERGILLGAVHG+VE+LFRRYTE GM E+ AYK Sbjct: 275 ALGWSVALGSPFTFATTLEQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYK 334
Query: 183 NTVEGITGVISKTISTKGMLAVYNSLDEQGKKDFEIAYSSSYYPCMDILYECYEDVASGN 362 NTVE ITGVISKTIST+GMLAVYN+L E GKK+FE AYS+S+YPCM+ILYECYEDVASG+ Sbjct: 335 NTVESITGVISKTISTQGMLAVYNALSEDGKKEFEKAYSASFYPCMEILYECYEDVASGS 394
Query: 363 EILFLSPPGRRFQEKEKKHAFPMGKIDKTRMWKVGERVRAVRPEGDLGPLYGFTAGVYIA 542 EI + GRRF EKE AFPMGKID+TRMWKVGERVR+ RP GDLGPLY FTAGV++A Sbjct: 395 EIRSVVLAGRRFYEKEGLPAFPMGKIDQTRMWKVGERVRSTRPAGDLGPLYPFTAGVFVA 454
Query: 543 LMMAQIEVLRKKGHSYSEMVNESVIEAVDSLNP 641 +MMAQIEVLRKKGHSYSE++NESVIE+VDSLNP Sbjct: 455 MMMAQIEVLRKKGHSYSEIINESVIESVDSLNP 487
>sp|Q05758|ILV5_ARATH Ketol-acid reductoisomerase, chloroplastic OS=Arabidopsis thaliana GN=At3g58610 PE=2 SV=2 Length = 591
Score = 348 bits (893), Expect = 1e-95 Identities = 171/213 (80%), Positives = 190/213 (89%) Frame = +3
Query: 3 ALGWSIALGSPFTFATTLEDEYRSDIYGERGILLGAVHGLVEALFRRYTEAGMPEEDAYK 182 ALGWS+ALGSPFTFATTLE EYRSDI+GERGILLGAVHG+VE+LFRRYTE GM E+ AYK Sbjct: 285 ALGWSVALGSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYK 344
Query: 183 NTVEGITGVISKTISTKGMLAVYNSLDEQGKKDFEIAYSSSYYPCMDILYECYEDVASGN 362 NTVE ITG IS+TIST+GMLAVYNSL E+GKKDFE AYS+S+YPCM+ILYECYEDV SG+ Sbjct: 345 NTVECITGTISRTISTQGMLAVYNSLSEEGKKDFETAYSASFYPCMEILYECYEDVQSGS 404
Query: 363 EILFLSPPGRRFQEKEKKHAFPMGKIDKTRMWKVGERVRAVRPEGDLGPLYGFTAGVYIA 542 EI + GRRF EKE AFPMG ID+TRMWKVGERVR RP GDLGPLY FTAGVY+A Sbjct: 405 EIRSVVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVA 464
Query: 543 LMMAQIEVLRKKGHSYSEMVNESVIEAVDSLNP 641 LMMAQIE+LRKKGHSYSE++NESVIE+VDSLNP Sbjct: 465 LMMAQIEILRKKGHSYSEIINESVIESVDSLNP 497
>sp|P06168|ILV5_YEAST Ketol-acid reductoisomerase, mitochondrial OS=Saccharomyces cerevisiae GN=ILV5 PE=1 SV=1 Length = 395
Score = 80.5 bits (197), Expect = 7e-15 Identities = 52/164 (31%), Positives = 84/164 (51%), Gaps = 4/164 (2%) Frame = +3
Query: 15 SIALGSPFTFATTLEDEYRSDIYGERGILLGAVHGLVEALFRRYTEAGMPEEDAYKNTVE 194 ++A+GS + + TT E E SD+YGERG L+G +HG+ A + E G +A+ TVE Sbjct: 235 AVAIGSGYVYQTTFEREVNSDLYGERGCLMGGIHGMFLAQYDVLRENGHSPSEAFNETVE 294
Query: 195 GITGVISKTISTKGMLAVYNSLD---EQGKKDFEIAYSSSYYPCMDILYECYED-VASGN 362 T + I GM +Y++ +G D+ + ++ P LYE ++ + Sbjct: 295 EATQSLYPLIGKYGMDYMYDACSTTARRGALDWYPIFKNALKPVFQDLYESTKNGTETKR 354
Query: 363 EILFLSPPGRRFQEKEKKHAFPMGKIDKTRMWKVGERVRAVRPE 494 + F S P ++EK +K + I +WKVG+ VR +RPE Sbjct: 355 SLEFNSQPD--YREKLEKE---LDTIRNMEIWKVGKEVRKLRPE 393
Score = 39.3 bits (90), Expect = 0.018 Identities = 23/49 (46%), Positives = 30/49 (61%) Frame = +3
Query: 495 GDLGPLYGFTAGVYIALMMAQIEVLRKKGHSYSEMVNESVIEAVDSLNP 641 G+ G L G G+++A Q +VLR+ GHS SE NE+V EA SL P Sbjct: 258 GERGCLMGGIHGMFLA----QYDVLRENGHSPSEAFNETVEEATQSLYP 302
>sp|P78827|ILV5_SCHPO Probable ketol-acid reductoisomerase, mitochondrial OS=Schizosaccharomyces pombe GN=ilv5 PE=1 SV=2 Length = 404
Score = 77.0 bits (188), Expect = 8e-14 Identities = 55/172 (31%), Positives = 88/172 (51%), Gaps = 8/172 (4%) Frame = +3
Query: 3 ALGWSIALGSPFTFATTLEDEYRSDIYGERGILLGAVHGLVEALFRRYTEAGMPEEDAYK 182 A+G ++A+GS F + TT + E SD+ GERG L+G ++GL A ++ E G +A+ Sbjct: 238 AIGLAVAVGSGFIYQTTFKKEVISDLVGERGCLMGGINGLFLAQYQVLRERGHSPAEAFN 297
Query: 183 NTVEGITGVISKTISTKG---MLAVYNSLDEQGKKDFEIAYSSSYYPCMDILYECYEDVA 353 TVE T + I G M A ++ +G D+ + + +L E Y++V Sbjct: 298 ETVEEATQSLYPLIGKYGLDYMFAACSTTARRGAIDWTPRFLEAN---KKVLNELYDNVE 354
Query: 354 SGNE----ILFLSPPG-RRFQEKEKKHAFPMGKIDKTRMWKVGERVRAVRPE 494 +GNE + + S P R +KE + +I +WK GE VR++RPE Sbjct: 355 NGNEAKRSLEYNSAPNYRELYDKE------LEEIRNLEIWKAGEVVRSLRPE 400
Score = 38.9 bits (89), Expect = 0.023 Identities = 22/49 (44%), Positives = 31/49 (63%) Frame = +3
Query: 495 GDLGPLYGFTAGVYIALMMAQIEVLRKKGHSYSEMVNESVIEAVDSLNP 641 G+ G L G G+++A Q +VLR++GHS +E NE+V EA SL P Sbjct: 265 GERGCLMGGINGLFLA----QYQVLRERGHSPAEAFNETVEEATQSLYP 309
>sp|P38674|ILV5_NEUCR Ketol-acid reductoisomerase, mitochondrial OS=Neurospora crassa GN=ilv-2 PE=3 SV=2 Length = 402
Score = 76.3 bits (186), Expect = 1e-13 Identities = 49/171 (28%), Positives = 86/171 (50%), Gaps = 7/171 (4%) Frame = +3
Query: 3 ALGWSIALGSPFTFATTLEDEYRSDIYGERGILLGAVHGLVEALFRRYTEAGMPEEDAYK 182 A+ +A+GS + + TT E E SD+YGERG L+G +HG+ A + E G +A+ Sbjct: 237 AVALGVAVGSGYLYETTFEKEVYSDLYGERGCLMGGIHGMFLAQYEVLRERGHSPSEAFN 296
Query: 183 NTVEGITGVISKTISTKGMLAVYNSLD---EQGKKDFEIAYSSSYYPCMDILYECYEDVA 353 TVE T + I GM ++++ +G D+ + + P + L Y+ V Sbjct: 297 ETVEEATQSLYPLIGAHGMDWMFDACSTTARRGAIDWTPKFKDALKPVFNNL---YDSVK 353
Query: 354 SGNE----ILFLSPPGRRFQEKEKKHAFPMGKIDKTRMWKVGERVRAVRPE 494 +G+E + + S P R +++ + +I +W+ G+ VR++RPE Sbjct: 354 NGDETKRSLEYNSQPDYR-----ERYEAELDEIRNLEIWRAGKAVRSLRPE 399
Score = 41.2 bits (95), Expect = 0.005 Identities = 24/49 (48%), Positives = 31/49 (63%) Frame = +3
Query: 495 GDLGPLYGFTAGVYIALMMAQIEVLRKKGHSYSEMVNESVIEAVDSLNP 641 G+ G L G G+++A Q EVLR++GHS SE NE+V EA SL P Sbjct: 264 GERGCLMGGIHGMFLA----QYEVLRERGHSPSEAFNETVEEATQSLYP 308
>sp|A6Q461|ILVC_NITSB Ketol-acid reductoisomerase OS=Nitratiruptor sp. (strain SB155-2) GN=ilvC PE=3 SV=1 Length = 340
Score = 49.3 bits (116), Expect = 2e-05 Identities = 22/44 (50%), Positives = 30/44 (68%) Frame = +3
Query: 48 TTLEDEYRSDIYGERGILLGAVHGLVEALFRRYTEAGMPEEDAY 179 TT +DE +D++GE+ +L G V L++A F TEAG PEE AY Sbjct: 183 TTFKDETETDLFGEQAVLCGGVSALIQAGFETLTEAGYPEEMAY 226
>sp|Q5SJ03|ILVC_THET8 Ketol-acid reductoisomerase OS=Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) GN=ilvC PE=3 SV=1 Length = 337
Score = 48.5 bits (114), Expect = 3e-05 Identities = 31/94 (32%), Positives = 48/94 (51%), Gaps = 4/94 (4%) Frame = +3
Query: 3 ALGWSIALGSPFT--FATTLEDEYRSDIYGERGILLGAVHGLVEALFRRYTEAGMPEEDA 176 AL ++ A+G+ ATT +DE +D++GE+ +L G + L+ A F EAG P E A Sbjct: 164 ALAYAKAIGAARAGVIATTFKDETETDLFGEQAVLCGGLTRLIRAGFETLVEAGYPPEMA 223
Query: 177 YKNTVEGITGVISKTIST--KGMLAVYNSLDEQG 272 Y TV + ++ KGM ++ E G Sbjct: 224 YFETVHEVKLIVDLIYEAGLKGMRYSISNTAEYG 257
>sp|Q72JC8|ILVC_THET2 Ketol-acid reductoisomerase OS=Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) GN=ilvC PE=3 SV=1 Length = 337
Score = 48.5 bits (114), Expect = 3e-05 Identities = 31/94 (32%), Positives = 48/94 (51%), Gaps = 4/94 (4%) Frame = +3
Query: 3 ALGWSIALGSPFT--FATTLEDEYRSDIYGERGILLGAVHGLVEALFRRYTEAGMPEEDA 176 AL ++ A+G+ ATT +DE +D++GE+ +L G + L+ A F EAG P E A Sbjct: 164 ALAYAKAIGAARAGVIATTFKDETETDLFGEQAVLCGGLTRLIRAGFETLVEAGYPPEMA 223
Query: 177 YKNTVEGITGVISKTIST--KGMLAVYNSLDEQG 272 Y TV + ++ KGM ++ E G Sbjct: 224 YFETVHEVKLIVDLIYEAGLKGMRYSISNTAEYG 257
>sp|Q7M851|ILVC_WOLSU Ketol-acid reductoisomerase OS=Wolinella succinogenes GN=ilvC PE=3 SV=1 Length = 340
Score = 48.1 bits (113), Expect = 4e-05 Identities = 26/60 (43%), Positives = 36/60 (60%), Gaps = 2/60 (3%) Frame = +3
Query: 6 LGWSIALGSPFT--FATTLEDEYRSDIYGERGILLGAVHGLVEALFRRYTEAGMPEEDAY 179 L ++ A+G T TT +DE +D++GE+ +L G V LV+A F EAG PEE AY Sbjct: 167 LSYASAIGGGRTGIIETTFKDETETDLFGEQAVLCGGVTALVKAGFETLVEAGYPEEMAY 226
|
TrEMBL (release 39.9) |
Link to BlastX Result : TrEMBL |
tr_hit_id |
A9TQQ1 |
Definition |
tr|A9TQQ1|A9TQQ1_PHYPA Predicted protein OS=Physcomitrella patens subsp. patens |
Align length |
213 |
Score (bit) |
354.0 |
E-value |
3.0e-96 |
Report |
|