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NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK962034
Clone id TST39A01NGRL0011_M11
Library
Length 620
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0011_M11. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence 
TTTCGCGCAGATTTGCTTTTGGGCTTCCTGCAAACACCTTCTTTTTGCCGCGGATCTCTG
TCAGACCTCGCAAAGCTCAAGGTCTGTTTTAGGGCATTTGATACATGCTGGATATGGTTA
TGGAGATGATAACCGGGGCAGATCCTTACACTCTAGCTGCGGTGGGGGCCTCGTTGATTG
TTGCAGTAGGCATCTACTATCTGGTTTTCATTCTGGGCCCTGCCGAAACAAAGGGCAAAT
CTGCAGTAGAGCTGGGAGGGGGCTCTCTTGCACGGGAGCAGGTCGCAGATAACTACAAGG
CCTATGCAGATTTTTTCAAAAAGCCTAAGGAGATTGAAAATGCAAAACAGGTTCCAAAAC
TTGTAGACACCTTCTACAACCTTGTGACTGACATCTATGAATGGGGCTGGGGACAGAGCT
TTCATTTTTCACCTGCAATTACAGGAAAATCCCATCGTGAGGAGACTAGACTTCATGAGG
AACACGTTGCAGATCTTCTGCACATTAAGCCCAAGGACAAGGTTCTTGATGCAGGTTGTG
GTGTGGGGGGCCCGATGCGTGCAATTGCGGCCCACTCGGGAGCGAATGTAGTGGGTATTA
CTATCAATGATTACCAGGTT
■■Homology search results ■■ -
sp_hit_id Q39227
Definition sp|Q39227|SMT2_ARATH 24-methylenesterol C-methyltransferase 2 OS=Arabidopsis thaliana
Align length 160
Score (bit) 232.0
E-value 1.0e-60
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK962034|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0011_M11, 5'
         (620 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q39227|SMT2_ARATH 24-methylenesterol C-methyltransferase 2 OS...   232   1e-60
sp|Q94JS4|SMT3B_ARATH 24-methylenesterol C-methyltransferase 3 O...   214   2e-55
sp|O82427|SMT2_ORYSJ 24-methylenesterol C-methyltransferase 2 OS...   207   4e-53
sp|Q6C2D9|ERG6_YARLI Sterol 24-C-methyltransferase OS=Yarrowia l...   105   1e-22
sp|Q54I98|SMT1_DICDI Probable cycloartenol-C-24-methyltransferas...   103   5e-22
sp|Q6ZIX2|SMT1_ORYSJ Cycloartenol-C-24-methyltransferase 1 OS=Or...   102   1e-21
sp|Q6FRZ7|ERG6_CANGA Sterol 24-C-methyltransferase OS=Candida gl...   100   1e-20
sp|O14321|ERG6_SCHPO Sterol 24-C-methyltransferase OS=Schizosacc...    99   1e-20
sp|Q5EN22|ERG6_MAGGR Sterol 24-C-methyltransferase OS=Magnaporth...    99   1e-20
sp|Q9LM02|SMT1_ARATH Cycloartenol-C-24-methyltransferase OS=Arab...    99   2e-20
sp|Q6BRB7|ERG6_DEBHA Sterol 24-C-methyltransferase OS=Debaryomyc...    99   2e-20
sp|P25087|ERG6_YEAST Sterol 24-C-methyltransferase OS=Saccharomy...    97   7e-20
sp|Q6CYB3|ERG6_KLULA Sterol 24-C-methyltransferase OS=Kluyveromy...    97   7e-20
sp|Q9P3R1|ERG6_NEUCR Sterol 24-C-methyltransferase OS=Neurospora...    96   2e-19
sp|O74198|ERG6_CANAL Sterol 24-C-methyltransferase OS=Candida al...    96   2e-19
sp|Q875K1|ERG6_CLALS Sterol 24-C-methyltransferase OS=Clavispora...    94   6e-19
sp|Q759S7|ERG6_ASHGO Sterol 24-C-methyltransferase OS=Ashbya gos...    94   6e-19
sp|Q96WX4|ERG6_PNECA Sterol 24-C-methyltransferase OS=Pneumocyst...    86   1e-16
sp|Q9ZSK1|GTOMC_ARATH Tocopherol O-methyltransferase, chloroplas...    50   7e-06
sp|Q6LMS7|RUMA_PHOPR 23S rRNA (uracil-5-)-methyltransferase rumA...    40   0.010
sp|Q9C6B9|PEAM3_ARATH Putative phosphoethanolamine N-methyltrans...    40   0.013
sp|P0A5P0|CFA2_MYCTU Cyclopropane-fatty-acyl-phospholipid syntha...    39   0.022
sp|P0A5P1|CFA2_MYCBO Cyclopropane-fatty-acyl-phospholipid syntha...    39   0.022
sp|P0A9H7|CFA_ECOLI Cyclopropane-fatty-acyl-phospholipid synthas...    39   0.028
sp|P0A9H8|CFA_ECOL6 Cyclopropane-fatty-acyl-phospholipid synthas...    39   0.028
sp|P31049|YLP3_PSEPU Uncharacterized methyltransferase in lpd-3 ...    37   0.11 
sp|Q9M571|PEAMT_SPIOL Phosphoethanolamine N-methyltransferase OS...    36   0.14 
sp|Q84BQ9|PRMA_THET8 Ribosomal protein L11 methyltransferase OS=...    36   0.18 
sp|Q944H0|PEAM2_ARATH Putative phosphoethanolamine N-methyltrans...    36   0.18 
sp|Q9FR44|PEAM1_ARATH Phosphoethanolamine N-methyltransferase 1 ...    36   0.18 

>sp|Q39227|SMT2_ARATH 24-methylenesterol C-methyltransferase 2
           OS=Arabidopsis thaliana GN=SMT2 PE=1 SV=2
          Length = 361

 Score =  232 bits (591), Expect = 1e-60
 Identities = 110/160 (68%), Positives = 130/160 (81%)
 Frame = +3

Query: 141 DPYTLAAVGASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFK 320
           D  TL   GA  +VAVGIY+ + +LGPAE KGK AV+L GGS++ E+V DNYK Y  FF+
Sbjct: 2   DSLTLFFTGA--LVAVGIYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFR 59

Query: 321 KPKEIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLL 500
           +PKEIE A++VP  VDTFYNLVTDIYEWGWGQSFHFSP+I GKSH++ TRLHEE   DL+
Sbjct: 60  RPKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLI 119

Query: 501 HIKPKDKVLDAGCGVGGPMRAIAAHSGANVVGITINDYQV 620
            +KP  K+LD GCGVGGPMRAIA+HS ANVVGITIN+YQV
Sbjct: 120 QVKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQV 159


>sp|Q94JS4|SMT3B_ARATH 24-methylenesterol C-methyltransferase 3
           OS=Arabidopsis thaliana GN=SMT3 PE=2 SV=1
          Length = 359

 Score =  214 bits (546), Expect = 2e-55
 Identities = 97/148 (65%), Positives = 119/148 (80%)
 Frame = +3

Query: 177 IVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPKEIENAKQVP 356
           ++A  +Y+ + +LGPAE KGK A +L GGS++ E+V DNY  Y  FF+KPKEIE+A++VP
Sbjct: 12  LIAGAVYWFICVLGPAERKGKRASDLSGGSISAEKVKDNYNQYWSFFRKPKEIESAEKVP 71

Query: 357 KLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIKPKDKVLDAG 536
             VDTFYNLVTDIYEWGWGQSFHFSP + GKS ++ TR+HEE   DL+ +KP  K+LDAG
Sbjct: 72  DFVDTFYNLVTDIYEWGWGQSFHFSPHVPGKSDKDATRIHEEMAVDLIKVKPGQKILDAG 131

Query: 537 CGVGGPMRAIAAHSGANVVGITINDYQV 620
           CGVGGPMRAIAAHS A V GITIN+YQV
Sbjct: 132 CGVGGPMRAIAAHSKAQVTGITINEYQV 159


>sp|O82427|SMT2_ORYSJ 24-methylenesterol C-methyltransferase 2
           OS=Oryza sativa subsp. japonica GN=Smt2-1 PE=2 SV=2
          Length = 363

 Score =  207 bits (527), Expect = 4e-53
 Identities = 104/171 (60%), Positives = 128/171 (74%), Gaps = 4/171 (2%)
 Frame = +3

Query: 120 MEMITGADPYTLAAVGASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYK 299
           ME  T A  +T A VG +L+     Y+ V+++G AE KGK AV+L  GS+  ++V D Y 
Sbjct: 1   MEAATMA--WTAAGVGMALV-----YWFVWVMGAAEVKGKRAVDLKMGSITNDKVKDKYT 53

Query: 300 AYADFFKKPKEIE----NAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREET 467
            Y  FF++PKE      +A++VP  VDTFYNLVTDIYEWGWGQSFHFSP++ G+SHRE T
Sbjct: 54  QYWSFFRRPKETATTEASAEKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHREAT 113

Query: 468 RLHEEHVADLLHIKPKDKVLDAGCGVGGPMRAIAAHSGANVVGITINDYQV 620
           R+HEE VADLL  KP  ++LD GCGVGGPMRAIAAHSG+NVVGITIN+YQV
Sbjct: 114 RVHEERVADLLQAKPGHRLLDVGCGVGGPMRAIAAHSGSNVVGITINEYQV 164


>sp|Q6C2D9|ERG6_YARLI Sterol 24-C-methyltransferase OS=Yarrowia
           lipolytica GN=ERG6 PE=3 SV=1
          Length = 381

 Score =  105 bits (263), Expect = 1e-22
 Identities = 53/107 (49%), Positives = 70/107 (65%), Gaps = 6/107 (5%)
 Frame = +3

Query: 318 KKPKEIENAKQVPKLVDT------FYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHE 479
           K   +IE+A +  ++ D       +YNLVTD YE+GWG SFHFS    G++ R+ T  HE
Sbjct: 58  KDRSKIEDADEQGRIEDYAGLTKHYYNLVTDFYEYGWGSSFHFSRYYKGEAFRQATARHE 117

Query: 480 EHVADLLHIKPKDKVLDAGCGVGGPMRAIAAHSGANVVGITINDYQV 620
            ++A  + I+P  KVLD GCGVGGP R IA  +GAN+VG+  NDYQV
Sbjct: 118 HYLAYKMGIQPGMKVLDVGCGVGGPAREIARFTGANIVGLNNNDYQV 164


>sp|Q54I98|SMT1_DICDI Probable cycloartenol-C-24-methyltransferase 1
           OS=Dictyostelium discoideum GN=smt1 PE=1 SV=1
          Length = 354

 Score =  103 bits (258), Expect = 5e-22
 Identities = 55/118 (46%), Positives = 72/118 (61%), Gaps = 3/118 (2%)
 Frame = +3

Query: 276 EQVADNYKAYADFFK--KPKEIENAKQ-VPKLVDTFYNLVTDIYEWGWGQSFHFSPAITG 446
           ++V D    Y + FK    K I+  K     +V+TFY+L TD YE+GWGQSFHF+     
Sbjct: 30  DKVDDTLNGYKELFKGNDDKAIQARKNNYTHMVNTFYDLATDFYEFGWGQSFHFATRHKY 89

Query: 447 KSHREETRLHEEHVADLLHIKPKDKVLDAGCGVGGPMRAIAAHSGANVVGITINDYQV 620
           +S       HE ++A  L + P  KV+D GCGVGGPMR IA  SGANVVG+  N+YQ+
Sbjct: 90  ESFEASIARHEMYMAHQLGLFPGMKVIDIGCGVGGPMRTIARFSGANVVGLNNNEYQI 147


>sp|Q6ZIX2|SMT1_ORYSJ Cycloartenol-C-24-methyltransferase 1 OS=Oryza
           sativa subsp. japonica GN=Smt1-1 PE=2 SV=1
          Length = 344

 Score =  102 bits (255), Expect = 1e-21
 Identities = 50/124 (40%), Positives = 76/124 (61%), Gaps = 3/124 (2%)
 Frame = +3

Query: 258 GGSLAREQV---ADNYKAYADFFKKPKEIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHF 428
           GG + +++V    D Y+ Y  ++   +E   +     +V+ +Y+L T  YE+GWG+SFHF
Sbjct: 14  GGKITKDEVKSAVDEYEKYHGYYGGKEEARKSNYTD-MVNKYYDLATSFYEYGWGESFHF 72

Query: 429 SPAITGKSHREETRLHEEHVADLLHIKPKDKVLDAGCGVGGPMRAIAAHSGANVVGITIN 608
           +    G+S RE  + HE  +A  L +KP  KVLD GCG+GGP+R IA  S A+V G+  N
Sbjct: 73  AHRWNGESLRESIKRHEHFLALQLGVKPGMKVLDVGCGIGGPLREIAKFSLASVTGLNNN 132

Query: 609 DYQV 620
           +YQ+
Sbjct: 133 EYQI 136


>sp|Q6FRZ7|ERG6_CANGA Sterol 24-C-methyltransferase OS=Candida
           glabrata GN=ERG6 PE=3 SV=1
          Length = 372

 Score = 99.8 bits (247), Expect = 1e-20
 Identities = 50/135 (37%), Positives = 74/135 (54%)
 Frame = +3

Query: 216 GPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPKEIENAKQVPKLVDTFYNLVTDI 395
           G     G +A+     +  RE V+   + +     K  E    +   +   ++YN+VTD 
Sbjct: 21  GAESRTGLTALLSKNRNAQREAVSKYLRHWDGKTDKEAEDRRLEDYNEATHSYYNVVTDF 80

Query: 396 YEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIKPKDKVLDAGCGVGGPMRAIAAH 575
           YE+GWG SFHFS    G++ +     HE+++A +  IK  D VLD GCGVGGP R IA  
Sbjct: 81  YEYGWGSSFHFSRFYKGENFQAAMARHEQYLAYMAGIKKGDLVLDVGCGVGGPARTIARF 140

Query: 576 SGANVVGITINDYQV 620
           +G N++G+  NDYQ+
Sbjct: 141 TGCNIIGLNNNDYQI 155


>sp|O14321|ERG6_SCHPO Sterol 24-C-methyltransferase
           OS=Schizosaccharomyces pombe GN=SPBC16E9.05 PE=2 SV=1
          Length = 378

 Score = 99.4 bits (246), Expect = 1e-20
 Identities = 51/140 (36%), Positives = 83/140 (59%), Gaps = 11/140 (7%)
 Frame = +3

Query: 234 GKSAVELGG-GSLAREQVADNYKAYADFFK-------KPKEIENAKQVP---KLVDTFYN 380
           GK+A +  G  ++A + V +  +   ++F+          E + A+++     +V+++Y+
Sbjct: 21  GKAAEKKTGLAAIASKDVDEQSRKLQEYFEFWDRNHENESEEDRARRIDGYKSVVNSYYD 80

Query: 381 LVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIKPKDKVLDAGCGVGGPMR 560
           L TD+YE+GW QSFHFS    G++  +    HE ++A  + IKP  +VLD GCGVGGP R
Sbjct: 81  LATDLYEYGWSQSFHFSRFYKGEAFAQSIARHEHYLAYRMGIKPGSRVLDVGCGVGGPAR 140

Query: 561 AIAAHSGANVVGITINDYQV 620
            I   +G N+VG+  NDYQ+
Sbjct: 141 EITEFTGCNLVGLNNNDYQI 160


>sp|Q5EN22|ERG6_MAGGR Sterol 24-C-methyltransferase OS=Magnaporthe
           grisea GN=ERG6 PE=2 SV=2
          Length = 390

 Score = 99.4 bits (246), Expect = 1e-20
 Identities = 57/140 (40%), Positives = 73/140 (52%), Gaps = 5/140 (3%)
 Frame = +3

Query: 216 GPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPKEIENAKQVPK-----LVDTFYN 380
           G +   G  +  LG    A +   D Y  + D      E    ++  K     L   +YN
Sbjct: 31  GSSANMGGLSSMLGKDREASKTAVDQYFRHWDGKTAKTETTKVREERKADYATLTRQYYN 90

Query: 381 LVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIKPKDKVLDAGCGVGGPMR 560
           LVTD YE+GWGQSFHF     G++       +E  +A  + IK   KVLD GCGVGGP R
Sbjct: 91  LVTDFYEYGWGQSFHFCTFAPGETFASAITRYEHTLAHRIGIKKGMKVLDIGCGVGGPAR 150

Query: 561 AIAAHSGANVVGITINDYQV 620
            IA  +GAN+ GITIN+YQV
Sbjct: 151 QIAKFTGANITGITINEYQV 170


>sp|Q9LM02|SMT1_ARATH Cycloartenol-C-24-methyltransferase
           OS=Arabidopsis thaliana GN=SMT1 PE=2 SV=1
          Length = 336

 Score = 98.6 bits (244), Expect = 2e-20
 Identities = 49/123 (39%), Positives = 70/123 (56%), Gaps = 2/123 (1%)
 Frame = +3

Query: 258 GGSLAREQVADNYKAYADF--FKKPKEIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHFS 431
           GG + +  V    + Y  +  F    E E       +V+ +Y+L T  YE+GWG+SFHF+
Sbjct: 8   GGKIDKSDVLTAVEKYEQYHVFHGGNEEERKANYTDMVNKYYDLATSFYEYGWGESFHFA 67

Query: 432 PAITGKSHREETRLHEEHVADLLHIKPKDKVLDAGCGVGGPMRAIAAHSGANVVGITIND 611
               G+S RE  + HE  +A  L I+P  KVLD GCG+GGP+R IA  S + V G+  N+
Sbjct: 68  QRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVVTGLNNNE 127

Query: 612 YQV 620
           YQ+
Sbjct: 128 YQI 130
tr_hit_id B8LK59
Definition tr|B8LK59|B8LK59_PICSI Putative uncharacterized protein OS=Picea sitchensis
Align length 154
Score (bit) 253.0
E-value 8.0e-66
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK962034|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0011_M11, 5'
         (620 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|B8LK59|B8LK59_PICSI Putative uncharacterized protein OS=Picea...   253   8e-66
tr|B0FDL8|B0FDL8_GOSHI 24-sterol C-methyltransferase OS=Gossypiu...   233   5e-60
tr|B0FDL7|B0FDL7_GOSHI 24-sterol C-methyltransferase OS=Gossypiu...   230   6e-59
tr|A9P9Q4|A9P9Q4_POPTR Putative uncharacterized protein OS=Popul...   230   6e-59
tr|A7P8G6|A7P8G6_VITVI Chromosome chr3 scaffold_8, whole genome ...   229   1e-58
tr|O24154|O24154_TOBAC S-adenosyl-methionine-sterol-C-methyltran...   228   3e-58
tr|O24153|O24153_TOBAC S-adenosyl-methionine-sterol-C-methyltran...   226   8e-58
tr|A9PE86|A9PE86_POPTR Putative uncharacterized protein OS=Popul...   226   1e-57
tr|A7NVP2|A7NVP2_VITVI Chromosome chr18 scaffold_1, whole genome...   222   2e-56
tr|A9T0X1|A9T0X1_PHYPA Predicted protein OS=Physcomitrella paten...   221   4e-56
tr|A9T0U8|A9T0U8_PHYPA Predicted protein OS=Physcomitrella paten...   219   1e-55
tr|A5BH10|A5BH10_VITVI Putative uncharacterized protein OS=Vitis...   219   1e-55
tr|A9TCR1|A9TCR1_PHYPA Predicted protein OS=Physcomitrella paten...   215   1e-54
tr|Q10S41|Q10S41_ORYSJ Os03g0136200 protein OS=Oryza sativa subs...   207   4e-52
tr|A3ADV8|A3ADV8_ORYSJ Putative uncharacterized protein OS=Oryza...   207   5e-52
tr|A2XC88|A2XC88_ORYSI Putative uncharacterized protein OS=Oryza...   207   5e-52
tr|B6TB41|B6TB41_MAIZE 24-methylenesterol C-methyltransferase 2 ...   206   1e-51
tr|B4FQZ7|B4FQZ7_MAIZE Putative uncharacterized protein OS=Zea m...   206   1e-51
tr|B4FC47|B4FC47_MAIZE Putative uncharacterized protein OS=Zea m...   206   1e-51
tr|B4FB47|B4FB47_MAIZE Putative uncharacterized protein OS=Zea m...   206   1e-51
tr|B6TJP4|B6TJP4_MAIZE 24-methylenesterol C-methyltransferase 2 ...   205   2e-51
tr|A8IJ34|A8IJ34_CHLRE Sterol-C24-methyltransferase OS=Chlamydom...   143   7e-33
tr|A4RUR2|A4RUR2_OSTLU Predicted protein OS=Ostreococcus lucimar...   114   4e-24
tr|O82720|O82720_TOBAC S-adenosyl-methionine-sterol-C-methyltran...   106   1e-21
tr|Q2QDF7|Q2QDF7_GOSHI 24-sterol C-methyltransferase OS=Gossypiu...   105   2e-21
tr|A7Q258|A7Q258_VITVI Chromosome chr13 scaffold_45, whole genom...   105   2e-21
tr|B8BTY9|B8BTY9_THAPS Predicted protein (Fragment) OS=Thalassio...   103   6e-21
tr|Q0D7V5|Q0D7V5_ORYSJ Os07g0206700 protein OS=Oryza sativa subs...   102   1e-20
tr|B2ZWE2|B2ZWE2_9STRA Sterol methyltransferase OS=Aphanomyces e...   102   1e-20
tr|A3BHN4|A3BHN4_ORYSJ Putative uncharacterized protein OS=Oryza...   102   1e-20

>tr|B8LK59|B8LK59_PICSI Putative uncharacterized protein OS=Picea
           sitchensis PE=2 SV=1
          Length = 369

 Score =  253 bits (645), Expect = 8e-66
 Identities = 119/154 (77%), Positives = 139/154 (90%)
 Frame = +3

Query: 159 AVGASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPKEIE 338
           AVG + ++ +G+Y++++++G AE +GK AVELGGGSL+R QVADNYK Y+ FF+KPKEIE
Sbjct: 10  AVGTA-VLGMGLYWILYVVGAAEQRGKRAVELGGGSLSRNQVADNYKQYSAFFEKPKEIE 68

Query: 339 NAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIKPKD 518
            A +VPKLVDTFYNLVTDIYEWGWGQSFHFSPA+ GKSH+EETRLHEE VADLL +KP D
Sbjct: 69  KADKVPKLVDTFYNLVTDIYEWGWGQSFHFSPALPGKSHKEETRLHEEFVADLLKLKPGD 128

Query: 519 KVLDAGCGVGGPMRAIAAHSGANVVGITINDYQV 620
           +VLDAGCGVGGPMRAIAAHSGANVVGITINDYQV
Sbjct: 129 RVLDAGCGVGGPMRAIAAHSGANVVGITINDYQV 162


>tr|B0FDL8|B0FDL8_GOSHI 24-sterol C-methyltransferase OS=Gossypium
           hirsutum GN=SMT2-2 PE=2 SV=1
          Length = 361

 Score =  233 bits (595), Expect = 5e-60
 Identities = 111/160 (69%), Positives = 130/160 (81%)
 Frame = +3

Query: 141 DPYTLAAVGASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFK 320
           DP  L   GA  ++A G+Y+ V +LGPAE KGK AV+L GGS++ E+V DNYK Y  FF+
Sbjct: 2   DPVGLFCTGA--LLACGLYWFVCLLGPAEQKGKRAVDLSGGSISAEKVQDNYKQYWSFFR 59

Query: 321 KPKEIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLL 500
           +PKEIE A++VP  VDTFYNLVTDIYEWGWGQSFHFSP+I GKSHR+ TRLHEE   DL+
Sbjct: 60  QPKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIRGKSHRDATRLHEEMAVDLI 119

Query: 501 HIKPKDKVLDAGCGVGGPMRAIAAHSGANVVGITINDYQV 620
            +KP D+VLD GCGVGGPMRAIAAHS ANVVGITIN+YQV
Sbjct: 120 EVKPGDRVLDVGCGVGGPMRAIAAHSRANVVGITINEYQV 159


>tr|B0FDL7|B0FDL7_GOSHI 24-sterol C-methyltransferase OS=Gossypium
           hirsutum GN=SMT2-1 PE=2 SV=1
          Length = 361

 Score =  230 bits (586), Expect = 6e-59
 Identities = 109/160 (68%), Positives = 129/160 (80%)
 Frame = +3

Query: 141 DPYTLAAVGASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFK 320
           D  +L   GA  ++A G+Y+ V +LGPAE KGK AV+L GGS++ E+V DNYK Y  FF+
Sbjct: 2   DSLSLFCTGA--LLAGGLYWFVCVLGPAEQKGKRAVDLSGGSISAEKVRDNYKQYWSFFR 59

Query: 321 KPKEIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLL 500
           +PKEIE A++VP  VDTFYNLVTDIYEWGWGQSFHFSP+I GKSHR+ TRLHEE   DL+
Sbjct: 60  RPKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLI 119

Query: 501 HIKPKDKVLDAGCGVGGPMRAIAAHSGANVVGITINDYQV 620
            +KP D++LD GCGVGGPMRAIAAHS A VVGITINDYQV
Sbjct: 120 DVKPGDRILDVGCGVGGPMRAIAAHSQAKVVGITINDYQV 159


>tr|A9P9Q4|A9P9Q4_POPTR Putative uncharacterized protein OS=Populus
           trichocarpa PE=2 SV=1
          Length = 364

 Score =  230 bits (586), Expect = 6e-59
 Identities = 109/157 (69%), Positives = 126/157 (80%)
 Frame = +3

Query: 150 TLAAVGASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPK 329
           +LA      ++A GIY+ V ILGPAE KGK AV+L GGS++ E+V DNYK Y  FF+ PK
Sbjct: 3   SLALFCTGALIAGGIYWFVCILGPAEQKGKRAVDLSGGSISAEKVQDNYKQYWSFFRSPK 62

Query: 330 EIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIK 509
           EIE A++VP  VDTFYNLVTDIYEWGWGQSFHFSP+I GKSH E TRLHEE   DL+++K
Sbjct: 63  EIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHLEATRLHEEMAVDLINVK 122

Query: 510 PKDKVLDAGCGVGGPMRAIAAHSGANVVGITINDYQV 620
           P D++LD GCGVGGPMRAIAAHS A VVGITINDYQV
Sbjct: 123 PGDRILDVGCGVGGPMRAIAAHSRAKVVGITINDYQV 159


>tr|A7P8G6|A7P8G6_VITVI Chromosome chr3 scaffold_8, whole genome
           shotgun sequence OS=Vitis vinifera GN=GSVIVT00036172001
           PE=4 SV=1
          Length = 356

 Score =  229 bits (584), Expect = 1e-58
 Identities = 108/157 (68%), Positives = 129/157 (82%)
 Frame = +3

Query: 150 TLAAVGASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPK 329
           +LA    + +VA G+Y+ VF+LG AE KGK AV L GGS++ ++V DNYK Y  FF++PK
Sbjct: 3   SLALFCTASLVAGGVYWFVFLLGAAERKGKRAVHLSGGSISGDKVRDNYKQYWSFFRRPK 62

Query: 330 EIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIK 509
           EIE A++VP  VDTFYNLVTDIYEWGWGQSFHFSP++ GKSHR+ TR+HEE  ADLL I+
Sbjct: 63  EIETAEKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSVAGKSHRDATRVHEEMAADLLGIR 122

Query: 510 PKDKVLDAGCGVGGPMRAIAAHSGANVVGITINDYQV 620
             D+VLDAGCGVGGPMRAIA HSGANVVGITIN+YQV
Sbjct: 123 AGDRVLDAGCGVGGPMRAIARHSGANVVGITINEYQV 159


>tr|O24154|O24154_TOBAC
           S-adenosyl-methionine-sterol-C-methyltransferase
           OS=Nicotiana tabacum PE=2 SV=1
          Length = 357

 Score =  228 bits (580), Expect = 3e-58
 Identities = 107/151 (70%), Positives = 124/151 (82%)
 Frame = +3

Query: 168 ASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPKEIENAK 347
           A+L+ A G+Y+ V ILG AE KGK AV+L GGS+ +E V DNYK Y  FF++PKEIE A 
Sbjct: 10  AALLAAGGLYWFVCILGSAEVKGKRAVQLSGGSIEKENVQDNYKQYWSFFRRPKEIETAD 69

Query: 348 QVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIKPKDKVL 527
           +VP  VDTFYNLVTDIYEWGWGQSFHFSP+I GKSHRE TR+HEE   DL+ +KP  ++L
Sbjct: 70  KVPAFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHREATRIHEEMAVDLIGVKPGARIL 129

Query: 528 DAGCGVGGPMRAIAAHSGANVVGITINDYQV 620
           DAGCGVGGPMRAIAAHS ANVVGITIN+YQV
Sbjct: 130 DAGCGVGGPMRAIAAHSRANVVGITINEYQV 160


>tr|O24153|O24153_TOBAC
           S-adenosyl-methionine-sterol-C-methyltransferase homolog
           (Fragment) OS=Nicotiana tabacum PE=2 SV=1
          Length = 352

 Score =  226 bits (576), Expect = 8e-58
 Identities = 108/148 (72%), Positives = 121/148 (81%)
 Frame = +3

Query: 177 IVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPKEIENAKQVP 356
           ++A G+Y+ V ILG AE KGK AV L GG +A+E+V D YK Y  FF++PKEIE  + VP
Sbjct: 7   LLAGGLYWFVCILGSAEIKGKRAVNLSGGFIAKEKVQDKYKQYWSFFRRPKEIETTENVP 66

Query: 357 KLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIKPKDKVLDAG 536
             VDTFYNLVTDIYEWGWGQSFHFSPAITGK HRE TRLHEE   DLL IKP  +VLDAG
Sbjct: 67  AFVDTFYNLVTDIYEWGWGQSFHFSPAITGKFHREATRLHEEMAVDLLGIKPGARVLDAG 126

Query: 537 CGVGGPMRAIAAHSGANVVGITINDYQV 620
           CGVGGPMRAIAAHSGAN+VGITIN+YQV
Sbjct: 127 CGVGGPMRAIAAHSGANIVGITINEYQV 154


>tr|A9PE86|A9PE86_POPTR Putative uncharacterized protein OS=Populus
           trichocarpa PE=2 SV=1
          Length = 364

 Score =  226 bits (575), Expect = 1e-57
 Identities = 106/157 (67%), Positives = 124/157 (78%)
 Frame = +3

Query: 150 TLAAVGASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPK 329
           +LA      ++A GIY+ V +LGPAE KGK AV+L GGS++ E V DNY  Y  FF++PK
Sbjct: 3   SLALFFTGALLAGGIYWFVCVLGPAEQKGKRAVDLSGGSISAENVQDNYDQYWSFFRRPK 62

Query: 330 EIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIK 509
           EIE  ++VP  VDTFYNLVTDIYEWGWGQSFHFSP+I GKSH E TRLHEE   DL+++K
Sbjct: 63  EIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHSEATRLHEEMAVDLINVK 122

Query: 510 PKDKVLDAGCGVGGPMRAIAAHSGANVVGITINDYQV 620
           P D++LD GCGVGGPMRAIAAHS A VVGITINDYQV
Sbjct: 123 PGDRILDVGCGVGGPMRAIAAHSRAKVVGITINDYQV 159


>tr|A7NVP2|A7NVP2_VITVI Chromosome chr18 scaffold_1, whole genome
           shotgun sequence OS=Vitis vinifera GN=GSVIVT00015513001
           PE=4 SV=1
          Length = 360

 Score =  222 bits (565), Expect = 2e-56
 Identities = 106/160 (66%), Positives = 125/160 (78%)
 Frame = +3

Query: 141 DPYTLAAVGASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFK 320
           D  TL    A  ++A G+Y+ V ILG AE  GK  V+L GGS++ E+V D YK Y  FF+
Sbjct: 2   DSLTLFCTAA--LLAGGLYWFVCILGSAEQTGKRGVDLSGGSISAEKVQDKYKQYWSFFR 59

Query: 321 KPKEIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLL 500
           +PKEIE A++VP  VDTFYNLVTDIYEWGWGQSFHFSPA+ GKSHR+ TRLHEE   DL+
Sbjct: 60  RPKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPAVPGKSHRDATRLHEEMAVDLI 119

Query: 501 HIKPKDKVLDAGCGVGGPMRAIAAHSGANVVGITINDYQV 620
            +KP D++LD GCGVGGPMRAIAAHS ANVVGITIN+YQV
Sbjct: 120 DVKPGDRILDVGCGVGGPMRAIAAHSRANVVGITINEYQV 159


>tr|A9T0X1|A9T0X1_PHYPA Predicted protein OS=Physcomitrella patens
           subsp. patens GN=PHYPADRAFT_86618 PE=4 SV=1
          Length = 349

 Score =  221 bits (562), Expect = 4e-56
 Identities = 103/147 (70%), Positives = 118/147 (80%)
 Frame = +3

Query: 180 VAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPKEIENAKQVPK 359
           +  G YY++ + G AE KGK AV+LGGGS+ REQV  +Y+ YA FF+KPK+IE    VP 
Sbjct: 4   LGAGAYYVLVLAGAAERKGKRAVDLGGGSITREQVRSSYQKYASFFEKPKQIEAKDNVPN 63

Query: 360 LVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIKPKDKVLDAGC 539
            VDTFYNLVTDIYEWGWGQSFHFSPA+ GKSH+E TRLHEE V DLL +KP  KVLD GC
Sbjct: 64  FVDTFYNLVTDIYEWGWGQSFHFSPAVPGKSHKESTRLHEERVVDLLGVKPGQKVLDVGC 123

Query: 540 GVGGPMRAIAAHSGANVVGITINDYQV 620
           GVGGPMRAIAA+S A+V GITINDYQV
Sbjct: 124 GVGGPMRAIAAYSQAHVTGITINDYQV 150