DK962296
Clone id TST39A01NGRL0013_H21
Library
Length 646
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0013_H21. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
CGGCTCTGCTTATCGCTTAGACTTGCGCAGGCAGAGCTCTCTGCTGCTGCAAGTCTAGGC
TAAGCTTAGCTGGGCTCTCCCCTCGCTCTTCCAGGAGCCTGCTCTCTCGCCTTTCTCCGA
TCTTGTGCCCTGTCGCGGTTTTTCTTTGTGCACCATTCTCAGGAATGGCTGCAATGGCAA
AGTCTTGTCTTGACAAGACAACTGCTTTACAGGCCTCACCGTTTAGGCAGGATGGAGTTG
CCTCCTCAGCGAAGTGTGGACAACATGCAATTCTAAGCATGCCCCTCCACCAGAGTAAAA
CCAGAAAGTTGAGACCTGCGAGTTTTGATAAGTTGCCAACACTAAGTTTGCGCAAGGGGA
AGGCTGTTTTGCCACATGGAACATGGAGGACTATATCAGCAATTGCGGCTCCTATCGCAC
CGCCCCTGAGTGATCCTTCGTACAGAGCAAAGCTGGCGGAAGAGTATGGCTTTTCTCAAA
TTGGTGAGCCCGTTCCTCCAAATGTGACACTGAAGAATGTGATGGACTCTTTGCCTCCAG
AGGTGTTTGAGATTAACAACATGAAGGCATGGAGGACTGTAGCTATCACGGTTACTGCCT
ATGCAGCAGGGCTTTTTATGATTGCAAAGGCACCCTGGTATCTTCT
■■Homology search results ■■ -
sp_hit_id P48627
Definition sp|P48627|FAD6C_BRANA Omega-6 fatty acid desaturase, chloroplastic OS=Brassica napus
Align length 67
Score (bit) 102.0
E-value 1.0e-21
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK962296|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0013_H21, 5'
(646 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|P48627|FAD6C_BRANA Omega-6 fatty acid desaturase, chloroplast... 102 1e-21
sp|P48628|FAD6C_SOYBN Omega-6 fatty acid desaturase, chloroplast... 100 1e-20
sp|P48629|FAD6C_SPIOL Omega-6 fatty acid desaturase, chloroplast... 98 4e-20
sp|P46312|FAD6C_ARATH Omega-6 fatty acid desaturase, chloroplast... 96 1e-19
sp|P20388|DESA_SYNY3 Fatty acid desaturase OS=Synechocystis sp. ... 41 0.005
sp|Q54794|DESA_SPIPL Fatty acid desaturase OS=Spirulina platensi... 40 0.014
sp|Q84WP6|NAC43_ARATH NAC domain-containing protein 43 OS=Arabid... 31 6.4

>sp|P48627|FAD6C_BRANA Omega-6 fatty acid desaturase, chloroplastic
OS=Brassica napus PE=2 SV=1
Length = 443

Score = 102 bits (255), Expect = 1e-21
Identities = 46/67 (68%), Positives = 59/67 (88%)
Frame = +3

Query: 444 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 623
R +LAE YGF QIG+ +P NVTLK++MD+LP EVFEI+++KAW++V I+VT+YA GLFMI
Sbjct: 80 REQLAESYGFKQIGQDLPDNVTLKDIMDTLPKEVFEIDDVKAWKSVLISVTSYALGLFMI 139

Query: 624 AKAPWYL 644
AKAPWYL
Sbjct: 140 AKAPWYL 146


>sp|P48628|FAD6C_SOYBN Omega-6 fatty acid desaturase, chloroplastic
OS=Glycine max PE=2 SV=1
Length = 424

Score = 99.8 bits (247), Expect = 1e-20
Identities = 44/68 (64%), Positives = 60/68 (88%)
Frame = +3

Query: 441 YRAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 620
YR +LAE+YGF Q+GEP+ +VTLK+V++ LP EVFEI+++KAW++V I+VT+YA GLFM
Sbjct: 78 YRKQLAEDYGFRQVGEPLSDDVTLKDVINPLPKEVFEIDDVKAWKSVLISVTSYALGLFM 137

Query: 621 IAKAPWYL 644
I+KAPWYL
Sbjct: 138 ISKAPWYL 145


>sp|P48629|FAD6C_SPIOL Omega-6 fatty acid desaturase, chloroplastic
OS=Spinacia oleracea GN=FAD6 PE=1 SV=1
Length = 447

Score = 97.8 bits (242), Expect = 4e-20
Identities = 43/65 (66%), Positives = 56/65 (86%)
Frame = +3

Query: 450 KLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMIAK 629
+LAE YGF QIGEP+P +VT+++++ SLP +VFEIN+ KAW TV I+VT+YA G+FMIAK
Sbjct: 87 QLAERYGFQQIGEPLPDDVTMRDIITSLPKQVFEINDTKAWGTVLISVTSYALGIFMIAK 146

Query: 630 APWYL 644
APWYL
Sbjct: 147 APWYL 151


>sp|P46312|FAD6C_ARATH Omega-6 fatty acid desaturase, chloroplastic
OS=Arabidopsis thaliana GN=FAD6 PE=1 SV=2
Length = 448

Score = 96.3 bits (238), Expect = 1e-19
Identities = 44/67 (65%), Positives = 57/67 (85%)
Frame = +3

Query: 444 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 623
R +LAE YGF QIGE +P NVTLK++MD+LP EVFEI+++KA ++V I+VT+Y GLFMI
Sbjct: 85 REQLAESYGFRQIGEDLPENVTLKDIMDTLPKEVFEIDDLKALKSVLISVTSYTLGLFMI 144

Query: 624 AKAPWYL 644
AK+PWYL
Sbjct: 145 AKSPWYL 151


>sp|P20388|DESA_SYNY3 Fatty acid desaturase OS=Synechocystis sp.
(strain PCC 6803) GN=desA PE=3 SV=1
Length = 351

Score = 41.2 bits (95), Expect = 0.005
Identities = 19/47 (40%), Positives = 28/47 (59%)
Frame = +3

Query: 501 NVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMIAKAPWY 641
++ L++++ +LP E FE KAW +V IT+ A A G I PWY
Sbjct: 23 DLKLQDIIKTLPKECFEKKASKAWASVLITLGAIAVGYLGIIYLPWY 69


>sp|Q54794|DESA_SPIPL Fatty acid desaturase OS=Spirulina platensis
GN=desA PE=3 SV=1
Length = 351

Score = 39.7 bits (91), Expect = 0.014
Identities = 21/52 (40%), Positives = 31/52 (59%), Gaps = 1/52 (1%)
Frame = +3

Query: 492 VPPNVTLK-NVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMIAKAPWYL 644
VP ++ L+ +++++LP VF + KAW TV I V G IA APW+L
Sbjct: 18 VPSDLPLEEDIINTLPSGVFVQDRYKAWMTVIINVVMVGLGWLGIAIAPWFL 69


>sp|Q84WP6|NAC43_ARATH NAC domain-containing protein 43
OS=Arabidopsis thaliana GN=EMB2301 PE=2 SV=2
Length = 365

Score = 30.8 bits (68), Expect = 6.4
Identities = 19/63 (30%), Positives = 32/63 (50%), Gaps = 1/63 (1%)
Frame = -2

Query: 204 QLSCQDKTLPLQPFLRMVHKEKPRQ-GTRSEKGERAGSWKSEGRAQLSLA*TCSSRELCL 28
Q C+ T P + HK+K GTR+ + AG WK+ GR ++ + + R + +
Sbjct: 62 QEMCKIGTTPQNDWYFFSHKDKKYPTGTRTNRATAAGFWKATGRDKIIYS---NGRRIGM 118

Query: 27 RKS 19
RK+
Sbjct: 119 RKT 121


tr_hit_id A9TJT1
Definition tr|A9TJT1|A9TJT1_PHYPA Predicted protein OS=Physcomitrella patens subsp. patens
Align length 67
Score (bit) 109.0
E-value 2.0e-22
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK962296|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0013_H21, 5'
(646 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|A9TJT1|A9TJT1_PHYPA Predicted protein OS=Physcomitrella paten... 109 2e-22
tr|A9NV95|A9NV95_PICSI Putative uncharacterized protein OS=Picea... 105 2e-21
tr|A9PGN3|A9PGN3_POPTR Putative uncharacterized protein OS=Popul... 105 2e-21
tr|Q6DUV9|Q6DUV9_BRANA Omega-6 desaturase OS=Brassica napus PE=2... 102 2e-20
tr|Q6DUV4|Q6DUV4_BRANA Omega-6 desaturase OS=Brassica napus PE=4... 102 2e-20
tr|Q6DT48|Q6DT48_ARALP AT4G30950 (Fragment) OS=Arabidopsis lyrat... 102 2e-20
tr|Q09GN7|Q09GN7_DESSO Omega-6 fatty acid desaturase (Chloroplas... 102 3e-20
tr|A9UKE1|A9UKE1_OLEEU Plastidial delta-12 oleate desaturase OS=... 101 4e-20
tr|A7XZU9|A7XZU9_9ROSI Chloroplast omega-6 fatty acid desaturase... 100 8e-20
tr|Q6Z9G4|Q6Z9G4_ORYSJ Putative Omega-6 fatty acid desaturase, c... 99 2e-19
tr|B8BB37|B8BB37_ORYSI Putative uncharacterized protein OS=Oryza... 99 2e-19
tr|A3BTJ3|A3BTJ3_ORYSJ Putative uncharacterized protein OS=Oryza... 99 2e-19
tr|B6TGK8|B6TGK8_MAIZE Putative uncharacterized protein OS=Zea m... 98 5e-19
tr|Q5U9G0|Q5U9G0_OLEEU Chloroplast fatty acid desaturase 6 OS=Ol... 94 5e-18
tr|Q9AXY4|Q9AXY4_BRAOL Omega 6 reductase (Fragment) OS=Brassica ... 89 3e-16
tr|A3QQP5|A3QQP5_MESVI Chloroplast omega-6 fatty acid desaturase... 86 2e-15
tr|Q9AXY2|Q9AXY2_BRANA Omega 6 reductase (Fragment) OS=Brassica ... 82 2e-14
tr|Q9AXY3|Q9AXY3_BRACM Omega 6 reductase (Fragment) OS=Brassica ... 80 8e-14
tr|Q9AXY5|Q9AXY5_BRANA Omega 6 reductase (Fragment) OS=Brassica ... 80 1e-13
tr|Q9SBU4|Q9SBU4_CHLSW Chloroplast w6 desaturase OS=Chlamydomona... 75 3e-12
tr|Q9C600|Q9C600_ARATH Omega 6 reductase (Fragment) OS=Arabidops... 75 4e-12
tr|O48663|O48663_CHLRE Chloroplast w6 desaturase (Omega-6-FAD, c... 67 9e-10
tr|A9SI95|A9SI95_PHYPA Predicted protein (Fragment) OS=Physcomit... 64 8e-09
tr|Q9ZSV8|Q9ZSV8_ACEAT Putative chloroplast desaturase (Fragment... 57 1e-06
tr|A9SCR5|A9SCR5_PHYPA Predicted protein (Fragment) OS=Physcomit... 56 2e-06
tr|Q55231|Q55231_SYNP2 Delta 12 desaturase OS=Synechococcus sp. ... 53 1e-05
tr|A7R2X6|A7R2X6_VITVI Chromosome undetermined scaffold_462, who... 53 2e-05
tr|B0C0E1|B0C0E1_ACAM1 Fatty acid desaturase (Delta(12) desatura... 49 3e-04
tr|B1WXI5|B1WXI5_CYAA5 Fatty acid desaturase OS=Cyanothece (stra... 48 4e-04
tr|A0ZMU5|A0ZMU5_NODSP Fatty acid desaturase OS=Nodularia spumig... 47 8e-04

>tr|A9TJT1|A9TJT1_PHYPA Predicted protein OS=Physcomitrella patens
subsp. patens GN=PHYPADRAFT_222981 PE=4 SV=1
Length = 454

Score = 109 bits (272), Expect = 2e-22
Identities = 49/67 (73%), Positives = 60/67 (89%)
Frame = +3

Query: 444 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 623
R +LAEEYGF+QIGEPVP +VTLK V+DS P EVFEIN++KAW+T+AI+V +Y+ GLFMI
Sbjct: 92 RQRLAEEYGFTQIGEPVPADVTLKKVIDSFPSEVFEINDLKAWKTIAISVASYSLGLFMI 151

Query: 624 AKAPWYL 644
AKAPWYL
Sbjct: 152 AKAPWYL 158


>tr|A9NV95|A9NV95_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 460

Score = 105 bits (263), Expect = 2e-21
Identities = 47/67 (70%), Positives = 60/67 (89%)
Frame = +3

Query: 444 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 623
R +++E+YGF+QIGEPVP N+ LKNV+DSLP EVFEI++ KAW+TVAI+V AY+ GLF+I
Sbjct: 98 RNRISEKYGFTQIGEPVPDNIILKNVIDSLPQEVFEIDDTKAWKTVAISVAAYSLGLFLI 157

Query: 624 AKAPWYL 644
AKAPWYL
Sbjct: 158 AKAPWYL 164


>tr|A9PGN3|A9PGN3_POPTR Putative uncharacterized protein OS=Populus
trichocarpa PE=2 SV=1
Length = 444

Score = 105 bits (262), Expect = 2e-21
Identities = 46/68 (67%), Positives = 60/68 (88%)
Frame = +3

Query: 441 YRAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 620
YR +LAE YGF +IGEP+P N+TLK VMD+LP +VFEI++MKAW++V ++VT+YA GLFM
Sbjct: 81 YRKQLAESYGFRKIGEPLPDNITLKGVMDTLPKKVFEIDDMKAWKSVLVSVTSYAFGLFM 140

Query: 621 IAKAPWYL 644
I+KAPWYL
Sbjct: 141 ISKAPWYL 148


>tr|Q6DUV9|Q6DUV9_BRANA Omega-6 desaturase OS=Brassica napus PE=2
SV=1
Length = 443

Score = 102 bits (255), Expect = 2e-20
Identities = 46/67 (68%), Positives = 59/67 (88%)
Frame = +3

Query: 444 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 623
R +LAE YGF QIG+ +P NVTLK++MD+LP EVFEI+++KAW++V I+VT+YA GLFMI
Sbjct: 80 REQLAESYGFKQIGQDLPDNVTLKDIMDTLPKEVFEIDDVKAWKSVLISVTSYALGLFMI 139

Query: 624 AKAPWYL 644
AKAPWYL
Sbjct: 140 AKAPWYL 146


>tr|Q6DUV4|Q6DUV4_BRANA Omega-6 desaturase OS=Brassica napus PE=4
SV=1
Length = 443

Score = 102 bits (254), Expect = 2e-20
Identities = 46/67 (68%), Positives = 59/67 (88%)
Frame = +3

Query: 444 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 623
R +LAE YGF QIG+ +P NVTLK++MD+LP EVFEI+++KAW++V I+VT+YA GLFMI
Sbjct: 80 REQLAESYGFKQIGQNLPDNVTLKDIMDTLPKEVFEIDDVKAWKSVLISVTSYALGLFMI 139

Query: 624 AKAPWYL 644
AKAPWYL
Sbjct: 140 AKAPWYL 146


>tr|Q6DT48|Q6DT48_ARALP AT4G30950 (Fragment) OS=Arabidopsis lyrata
subsp. petraea PE=4 SV=1
Length = 203

Score = 102 bits (254), Expect = 2e-20
Identities = 46/67 (68%), Positives = 58/67 (86%)
Frame = +3

Query: 444 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 623
R +LAE YGF QIGE +P NVTLK++MD+LP EVFEI+++KAW++V I+VT+Y GLFMI
Sbjct: 50 REQLAESYGFKQIGENLPDNVTLKDIMDTLPKEVFEIDDLKAWKSVLISVTSYTLGLFMI 109

Query: 624 AKAPWYL 644
AKAPWYL
Sbjct: 110 AKAPWYL 116


>tr|Q09GN7|Q09GN7_DESSO Omega-6 fatty acid desaturase (Chloroplast
delta-12 fatty acid desaturase) OS=Descurainia sophia
GN=FAD6 PE=2 SV=1
Length = 447

Score = 102 bits (253), Expect = 3e-20
Identities = 46/67 (68%), Positives = 58/67 (86%)
Frame = +3

Query: 444 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 623
R +LAE YGF QIGE +P NVTLK++MD+LP EVFEI+++KAW++V I+VT+Y GLFMI
Sbjct: 84 REQLAESYGFRQIGEDLPDNVTLKDIMDTLPKEVFEIDDVKAWKSVLISVTSYTLGLFMI 143

Query: 624 AKAPWYL 644
AKAPWYL
Sbjct: 144 AKAPWYL 150


>tr|A9UKE1|A9UKE1_OLEEU Plastidial delta-12 oleate desaturase
OS=Olea europaea GN=FAD6 PE=2 SV=1
Length = 443

Score = 101 bits (251), Expect = 4e-20
Identities = 44/67 (65%), Positives = 60/67 (89%)
Frame = +3

Query: 444 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 623
R +L+E YGF QIGEP+P NVTLK+++D+LP +VFEI+++KAW++V I+VT+YA G+FMI
Sbjct: 81 RKQLSENYGFRQIGEPLPDNVTLKDIVDTLPRKVFEIDDVKAWKSVLISVTSYALGIFMI 140

Query: 624 AKAPWYL 644
AKAPWYL
Sbjct: 141 AKAPWYL 147


>tr|A7XZU9|A7XZU9_9ROSI Chloroplast omega-6 fatty acid desaturase
OS=Jatropha curcas PE=2 SV=1
Length = 440

Score = 100 bits (249), Expect = 8e-20
Identities = 42/68 (61%), Positives = 59/68 (86%)
Frame = +3

Query: 441 YRAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 620
YR +LAE YGF QIGEP+P N+TL+ ++D+LP +VFEI+++KAW++V ++VT+YA GL M
Sbjct: 77 YRKQLAESYGFRQIGEPLPENITLREIIDTLPKKVFEIDDVKAWKSVLVSVTSYALGLLM 136

Query: 621 IAKAPWYL 644
I+KAPWYL
Sbjct: 137 ISKAPWYL 144


>tr|Q6Z9G4|Q6Z9G4_ORYSJ Putative Omega-6 fatty acid desaturase,
chloroplast OS=Oryza sativa subsp. japonica
GN=P0528B09.19 PE=4 SV=1
Length = 414

Score = 99.0 bits (245), Expect = 2e-19
Identities = 43/67 (64%), Positives = 58/67 (86%)
Frame = +3

Query: 444 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 623
R ++ E+YGF QIGE +P NVTLK+VMDSLP EVFEI+++K+W +V I+VT+YA G+F+I
Sbjct: 52 RKQMCEDYGFKQIGEQLPDNVTLKDVMDSLPKEVFEIDDLKSWTSVLISVTSYALGIFLI 111

Query: 624 AKAPWYL 644
+KAPWYL
Sbjct: 112 SKAPWYL 118