DK963052
Clone id TST39A01NGRL0015_I01
Library
Length 646
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0015_I01. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
GGAGAATGAGATCTCTAACCGCGTGCCTGTTGAAGAATGAGCCGGCGACTTGTAGGCGGT
GGCCCGGTTAAGGGGGAGAACCCCCGGAGCCGTAGCGAAAGCGAGTCTTGCTGGGGCAAT
TGTCACCGCTTATAAACCCGAACCTGGGTGATCTATCCATGACCAGGATGAAGCTTGGGT
GAAACTAAGTGGAGGTCCGAACCGACTGATGTTGAAAAATCAGCGGATGAGTTGTGGTTA
GGGGTGAAATGCCACTCGAACCCAGAGCTAGCTGGTTCTCCCCGAAATGCGTTGAGGCGC
AGCAGTTGACTAGACCATCTAGGGGTAAAGCACCGTTCCGGTGCGGGCTGCGAGAGCGGT
ACCAAATCGAGGCAAACTCTGAATACTAGATGTAACGTTCAAGTAAAAGGAACGAAGGAA
AAGGGTCAACCAGTGAGACGATGAGGGATAAGCTTCACCGTCGAGAGGGGAACAGCCCAT
ATCACCAGCTAAGGCCCCTAAATGACCGCTCAGTGGTAAAGGAGGTAGGAGTGCAAAGAC
AGCCAGGAGGTTTGCCTAGAAGCAGCCACCCTTGAAAGAGTGCGTAATAGCTCACTGATC
AAGCGCTCTTGCGCCGAAGATGAGCGGGACTAAGCGGTCTGCCGAA
■■Homology search results ■■ -
sp_hit_id Q99819
Definition sp|Q99819|GDIR3_HUMAN Rho GDP-dissociation inhibitor 3 OS=Homo sapiens
Align length 26
Score (bit) 30.8
E-value 6.3
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK963052|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0015_I01, 5'
(646 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|Q99819|GDIR3_HUMAN Rho GDP-dissociation inhibitor 3 OS=Homo s... 31 6.3
sp|Q88AR1|GSH1_PSESM Glutamate--cysteine ligase OS=Pseudomonas s... 31 6.4
sp|Q48PX1|GSH1_PSE14 Glutamate--cysteine ligase OS=Pseudomonas s... 31 6.4
sp|A2A8U2|TM201_MOUSE Transmembrane protein 201 OS=Mus musculus ... 30 8.3
sp|Q4ZZU4|GSH1_PSEU2 Glutamate--cysteine ligase OS=Pseudomonas s... 30 8.3
sp|P61379|GSH1_PSESY Glutamate--cysteine ligase OS=Pseudomonas s... 30 8.3

>sp|Q99819|GDIR3_HUMAN Rho GDP-dissociation inhibitor 3 OS=Homo
sapiens GN=ARHGDIG PE=2 SV=2
Length = 225

Score = 30.8 bits (68), Expect = 6.3
Identities = 13/26 (50%), Positives = 20/26 (76%)
Frame = +3

Query: 99 KRVLLGQLSPLINPNLGDLSMTRMKL 176
KRVLLG L P ++P+L ++ +TR+ L
Sbjct: 73 KRVLLGPLPPAVDPSLPNVQVTRLTL 98


>sp|Q88AR1|GSH1_PSESM Glutamate--cysteine ligase OS=Pseudomonas
syringae pv. tomato GN=gshA PE=3 SV=1
Length = 529

Score = 30.8 bits (68), Expect = 6.4
Identities = 17/43 (39%), Positives = 24/43 (55%)
Frame = +2

Query: 104 SLAGAIVTAYKPEPG*SIHDQDEAWVKLSGGPNRLMLKNQRMS 232
SL A+ T YKP HDQ+ WV+L+ N L ++N+ S
Sbjct: 263 SLRKAVATPYKPYVDIGTHDQNGEWVQLN--TNVLQIENEYYS 303


>sp|Q48PX1|GSH1_PSE14 Glutamate--cysteine ligase OS=Pseudomonas
syringae pv. phaseolicola (strain 1448A / Race 6)
GN=gshA PE=3 SV=1
Length = 535

Score = 30.8 bits (68), Expect = 6.4
Identities = 17/43 (39%), Positives = 24/43 (55%)
Frame = +2

Query: 104 SLAGAIVTAYKPEPG*SIHDQDEAWVKLSGGPNRLMLKNQRMS 232
SL A+ T YKP HDQ+ WV+L+ N L ++N+ S
Sbjct: 269 SLRKAVATPYKPYVDVGTHDQNGEWVQLN--TNVLQIENEYYS 309


>sp|A2A8U2|TM201_MOUSE Transmembrane protein 201 OS=Mus musculus
GN=Tmem201 PE=2 SV=1
Length = 664

Score = 30.4 bits (67), Expect = 8.3
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = -2

Query: 129 AVTIAPARLAFATAPGVLPLNRATAYKSPAHSS 31
+VT +PA L T PG LPL + ++SP S
Sbjct: 409 SVTSSPASLFIPTPPGFLPLTKQQLFRSPRRVS 441


>sp|Q4ZZU4|GSH1_PSEU2 Glutamate--cysteine ligase OS=Pseudomonas
syringae pv. syringae (strain B728a) GN=gshA PE=3 SV=1
Length = 535

Score = 30.4 bits (67), Expect = 8.3
Identities = 17/43 (39%), Positives = 24/43 (55%)
Frame = +2

Query: 104 SLAGAIVTAYKPEPG*SIHDQDEAWVKLSGGPNRLMLKNQRMS 232
SL A+ T YKP HDQ+ WV+L+ N L ++N+ S
Sbjct: 269 SLRKAVATPYKPYVEVGTHDQNGEWVQLN--TNVLQIENEYYS 309


>sp|P61379|GSH1_PSESY Glutamate--cysteine ligase OS=Pseudomonas
syringae pv. syringae GN=gshA PE=3 SV=1
Length = 535

Score = 30.4 bits (67), Expect = 8.3
Identities = 17/43 (39%), Positives = 24/43 (55%)
Frame = +2

Query: 104 SLAGAIVTAYKPEPG*SIHDQDEAWVKLSGGPNRLMLKNQRMS 232
SL A+ T YKP HDQ+ WV+L+ N L ++N+ S
Sbjct: 269 SLRKAVATPYKPYVEVGTHDQNGEWVQLN--TNVLQIENEYYS 309


tr_hit_id Q5M9Z9
Definition tr|Q5M9Z9|Q5M9Z9_TOBAC Putative uncharacterized protein orf106b OS=Nicotiana tabacum
Align length 37
Score (bit) 77.4
E-value 3.0e-34
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK963052|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0015_I01, 5'
(646 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|Q5M9Z9|Q5M9Z9_TOBAC Putative uncharacterized protein orf106b ... 77 3e-34
tr|A6N1H4|A6N1H4_ORYSI Retrotransposon protein OS=Oryza sativa s... 134 4e-30
tr|B7FGU0|B7FGU0_MEDTR Putative uncharacterized protein OS=Medic... 125 2e-27
tr|A7QCF1|A7QCF1_VITVI Chromosome undetermined scaffold_77, whol... 77 1e-19
tr|Q85WT3|Q85WT3_PINKO ORF46f OS=Pinus koraiensis PE=4 SV=1 87 7e-16
tr|A5ZTV7|A5ZTV7_9FIRM Putative uncharacterized protein OS=Rumin... 79 2e-13
tr|A5ZQM1|A5ZQM1_9FIRM Putative uncharacterized protein OS=Rumin... 79 2e-13
tr|A5KL06|A5KL06_9FIRM Putative uncharacterized protein OS=Rumin... 77 1e-12
tr|A7B8Q0|A7B8Q0_RUMGN Putative uncharacterized protein OS=Rumin... 72 4e-12
tr|Q9RXQ4|Q9RXQ4_DEIRA Putative uncharacterized protein OS=Deino... 74 8e-12
tr|Q9RS75|Q9RS75_DEIRA Putative uncharacterized protein OS=Deino... 74 8e-12
tr|B1BC32|B1BC32_CLOBO Putative uncharacterized protein OS=Clost... 45 5e-10
tr|Q85WS9|Q85WS9_PINKO ORF40u OS=Pinus koraiensis PE=4 SV=2 65 3e-09
tr|A7PNF4|A7PNF4_VITVI Chromosome chr1 scaffold_22, whole genome... 65 4e-09
tr|A5KR91|A5KR91_9FIRM Putative uncharacterized protein OS=Rumin... 62 4e-09
tr|A7QY24|A7QY24_VITVI Chromosome undetermined scaffold_237, who... 52 1e-08
tr|B0A7G6|B0A7G6_9CLOT Putative uncharacterized protein OS=Clost... 62 2e-08
tr|A5KRH1|A5KRH1_9FIRM Putative uncharacterized protein OS=Rumin... 62 3e-08
tr|B2PX02|B2PX02_PROST Putative uncharacterized protein OS=Provi... 62 4e-08
tr|A7B5W8|A7B5W8_RUMGN Putative uncharacterized protein OS=Rumin... 57 8e-08
tr|Q0JIM3|Q0JIM3_ORYSJ Photosystem I P700 chlorophyll A apoprote... 60 1e-07
tr|B0NEY0|B0NEY0_EUBSP Putative uncharacterized protein OS=Clost... 54 5e-07
tr|B3D9S7|B3D9S7_BURM1 Putative uncharacterized protein OS=Burkh... 58 6e-07
tr|A2PD01|A2PD01_VIBCH Putative uncharacterized protein OS=Vibri... 57 7e-07
tr|A8URE2|A8URE2_9AQUI Putative uncharacterized protein OS=Hydro... 55 5e-06
tr|Q3BKI1|Q3BKI1_9PROT Putative uncharacterized protein OS=Magne... 53 1e-05
tr|A9PE47|A9PE47_POPTR Putative uncharacterized protein OS=Popul... 53 1e-05
tr|A4ECR2|A4ECR2_9ACTN Putative uncharacterized protein OS=Colli... 53 2e-05
tr|B4VCZ2|B4VCZ2_9ACTO Putative uncharacterized protein OS=Strep... 53 2e-05
tr|B1BE08|B1BE08_CLOBO Putative uncharacterized protein OS=Clost... 51 4e-05

>tr|Q5M9Z9|Q5M9Z9_TOBAC Putative uncharacterized protein orf106b
OS=Nicotiana tabacum GN=orf106b PE=4 SV=1
Length = 106

Score = 77.4 bits (189), Expect(3) = 3e-34
Identities = 35/37 (94%), Positives = 36/37 (97%)
Frame = -1

Query: 310 VNCCASTHFGENQLALGSSGISPLTTTHPLIFQHQSV 200
VNCCASTHFGENQLALG+SGISPLTTTHPLI QHQSV
Sbjct: 48 VNCCASTHFGENQLALGASGISPLTTTHPLILQHQSV 84



Score = 60.8 bits (146), Expect(3) = 3e-34
Identities = 31/47 (65%), Positives = 35/47 (74%)
Frame = -3

Query: 458 VKLIPHRLTG*PFSFVPFT*TLHLVFRVCLDLVPLSQPAPERCFTPR 318
+KLIPHRLT P P +LVFRVCLDLVPLS+PAP+ CFTPR
Sbjct: 1 MKLIPHRLTSRPCP--PLILRSYLVFRVCLDLVPLSRPAPKPCFTPR 45



Score = 52.0 bits (123), Expect(3) = 3e-34
Identities = 21/21 (100%), Positives = 21/21 (100%)
Frame = -2

Query: 198 GPPLSFTQASSWSWIDHPGSG 136
GPPLSFTQASSWSWIDHPGSG
Sbjct: 85 GPPLSFTQASSWSWIDHPGSG 105


>tr|A6N1H4|A6N1H4_ORYSI Retrotransposon protein OS=Oryza sativa
subsp. indica PE=4 SV=1
Length = 109

Score = 134 bits (338), Expect = 4e-30
Identities = 75/114 (65%), Positives = 80/114 (70%), Gaps = 2/114 (1%)
Frame = +3

Query: 249 MPLEPRASWFSPKCVEAQQLTRPSRGKAPFRCGLRE--RYQIEANSEY*M*RSSKRNEGK 422
MPLEPRASWFSPKCVEAQQLT K F G + + N+ Y + G
Sbjct: 1 MPLEPRASWFSPKCVEAQQLTGHLGVKHCFGAGCASGTKSRQTLNTRY-----DPKITGV 55

Query: 423 GSTSETMRDKLHRREGNSPYHQLRPLNDRSVVKEVGVQRQPGGLPRSSHP*KSA 584
S SETM DKLHRREGNSP HQLRPLNDRSV+KEVGVQRQPGGLPRSSHP KSA
Sbjct: 56 RSASETMGDKLHRREGNSPDHQLRPLNDRSVIKEVGVQRQPGGLPRSSHPLKSA 109


>tr|B7FGU0|B7FGU0_MEDTR Putative uncharacterized protein OS=Medicago
truncatula PE=4 SV=1
Length = 102

Score = 125 bits (314), Expect = 2e-27
Identities = 69/108 (63%), Positives = 76/108 (70%)
Frame = +3

Query: 249 MPLEPRASWFSPKCVEAQQLTRPSRGKAPFRCGLRERYQIEANSEY*M*RSSKRNEGKGS 428
MPLEPRASWFSPKCVEAQQLT K F G E+ ++ +++ S
Sbjct: 1 MPLEPRASWFSPKCVEAQQLTGHLGVKHCFGAGR------ESGTKSRQTLNTRYPWRVKS 54

Query: 429 TSETMRDKLHRREGNSPYHQLRPLNDRSVVKEVGVQRQPGGLPRSSHP 572
SE M DKLHRREGNSP HQLRPLNDRSV+KEVGVQRQPGGLPRSSHP
Sbjct: 55 ASEAMGDKLHRREGNSPDHQLRPLNDRSVIKEVGVQRQPGGLPRSSHP 102


>tr|A7QCF1|A7QCF1_VITVI Chromosome undetermined scaffold_77, whole
genome shotgun sequence OS=Vitis vinifera
GN=GSVIVT00035369001 PE=4 SV=1
Length = 235

Score = 77.0 bits (188), Expect(2) = 1e-19
Identities = 37/41 (90%), Positives = 38/41 (92%)
Frame = -1

Query: 310 VNCCASTHFGENQLALGSSGISPLTTTHPLIFQHQSVRTST 188
VN CASTHFGENQLALGSS ISPLTTTHPLI QHQSV+TST
Sbjct: 57 VNRCASTHFGENQLALGSSDISPLTTTHPLILQHQSVQTST 97



Score = 43.5 bits (101), Expect(2) = 1e-19
Identities = 18/23 (78%), Positives = 22/23 (95%)
Frame = -3

Query: 386 VFRVCLDLVPLSQPAPERCFTPR 318
+FRVCLDLVPLS+PA ++CFTPR
Sbjct: 32 MFRVCLDLVPLSRPALKQCFTPR 54


>tr|Q85WT3|Q85WT3_PINKO ORF46f OS=Pinus koraiensis PE=4 SV=1
Length = 46

Score = 87.4 bits (215), Expect = 7e-16
Identities = 42/46 (91%), Positives = 42/46 (91%)
Frame = +3

Query: 249 MPLEPRASWFSPKCVEAQQLTRPSRGKAPFRCGLRERYQIEANSEY 386
MPLEPRASWFSPKCVEAQQLT PSRGKA FR G RERYQIEANSEY
Sbjct: 1 MPLEPRASWFSPKCVEAQQLTGPSRGKALFRYGPRERYQIEANSEY 46


>tr|A5ZTV7|A5ZTV7_9FIRM Putative uncharacterized protein
OS=Ruminococcus obeum ATCC 29174 GN=RUMOBE_02439 PE=4
SV=1
Length = 199

Score = 79.3 bits (194), Expect = 2e-13
Identities = 43/80 (53%), Positives = 52/80 (65%)
Frame = -2

Query: 324 P*MV*STAAPQRISGRTS*LWVRVAFHP*PQLIR*FFNISRFGPPLSFTQASSWSWIDHP 145
P + + A+P+ IS RTS L VR+ F P P LI FN FGPPL T AS+W+WIDHP
Sbjct: 11 PPQISTKASPKAISRRTSYLRVRLEFLPYPHLIPTLFNGCGFGPPLPLTAASTWTWIDHP 70

Query: 144 GSGL*AVTIAPARLAFATAP 85
SGL +T+A RL F TAP
Sbjct: 71 VSGLPPLTLALLRLGFPTAP 90


>tr|A5ZQM1|A5ZQM1_9FIRM Putative uncharacterized protein
OS=Ruminococcus obeum ATCC 29174 GN=RUMOBE_01293 PE=4
SV=1
Length = 199

Score = 79.3 bits (194), Expect = 2e-13
Identities = 43/80 (53%), Positives = 52/80 (65%)
Frame = -2

Query: 324 P*MV*STAAPQRISGRTS*LWVRVAFHP*PQLIR*FFNISRFGPPLSFTQASSWSWIDHP 145
P + + A+P+ IS RTS L VR+ F P P LI FN FGPPL T AS+W+WIDHP
Sbjct: 11 PPQISTKASPKAISRRTSYLRVRLEFLPYPHLIPTLFNGCGFGPPLPLTAASTWTWIDHP 70

Query: 144 GSGL*AVTIAPARLAFATAP 85
SGL +T+A RL F TAP
Sbjct: 71 VSGLPPLTLALLRLGFPTAP 90


>tr|A5KL06|A5KL06_9FIRM Putative uncharacterized protein
OS=Ruminococcus torques ATCC 27756 GN=RUMTOR_00910 PE=4
SV=1
Length = 245

Score = 76.6 bits (187), Expect = 1e-12
Identities = 41/73 (56%), Positives = 48/73 (65%)
Frame = -2

Query: 303 AAPQRISGRTS*LWVRVAFHP*PQLIR*FFNISRFGPPLSFTQASSWSWIDHPGSGL*AV 124
A+P+ IS RTS L VR+ F P P LI FN FGPPL FT S+W+WIDHP SGL +
Sbjct: 4 ASPKAISRRTSYLRVRLEFLPYPHLIPTLFNGCGFGPPLPFTATSTWTWIDHPVSGLLRL 63

Query: 123 TIAPARLAFATAP 85
T A RL F +AP
Sbjct: 64 TFALFRLGFPSAP 76


>tr|A7B8Q0|A7B8Q0_RUMGN Putative uncharacterized protein
OS=Ruminococcus gnavus ATCC 29149 GN=RUMGNA_03973 PE=4
SV=1
Length = 81

Score = 71.6 bits (174), Expect(2) = 4e-12
Identities = 37/64 (57%), Positives = 44/64 (68%)
Frame = -2

Query: 303 AAPQRISGRTS*LWVRVAFHP*PQLIR*FFNISRFGPPLSFTQASSWSWIDHPGSGL*AV 124
A+P+ IS RTS L VR+ F P P LI FN FGPPL FT S+W+WIDHP SGL +
Sbjct: 4 ASPKAISRRTSYLRVRLEFLPYPHLIPTLFNGCGFGPPLPFTATSTWTWIDHPVSGLLRL 63

Query: 123 TIAP 112
TI+P
Sbjct: 64 TISP 67



Score = 23.9 bits (50), Expect(2) = 4e-12
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = -3

Query: 116 PQQDSLSLRLRGFSP 72
P DS+SLRL+ FSP
Sbjct: 67 PCSDSVSLRLQAFSP 81


>tr|Q9RXQ4|Q9RXQ4_DEIRA Putative uncharacterized protein
OS=Deinococcus radiodurans GN=DR_0254 PE=4 SV=1
Length = 139

Score = 73.9 bits (180), Expect = 8e-12
Identities = 35/49 (71%), Positives = 37/49 (75%)
Frame = -1

Query: 634 LSPAHLRRKSA*SVSYYALFQGWLLLGKPPGCLCTPTSFTTERSFRGLS 488
LSP H RR VSYYALF+GWLLL +PPGCLC TS TTERSFR LS
Sbjct: 91 LSPDHFRRIVTRPVSYYALFEGWLLLSQPPGCLCDDTSLTTERSFRDLS 139