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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK963104
Clone id TST39A01NGRL0015_K05
Library
Length 636
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0015_K05. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence 
GTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATGGCTCTGTATTCC
GACGAAGATGCTGTTCCTAGTGAGGATATGGAGCTTGATGGTCGCATTGTCAAGGCGACC
CTCTCTCGTACCGGAGTCATTCGCTGGACAAGCTCTTCTTTTTCAGATAGCCTGGTCTTG
CAGGACGATCTCCTCGGCTTCTCCCGTGACCACTCTTCTCTCACTCTTTTCACTTTCAAG
GTAGGTCAGGTTGGCTGTTGTGCTTTGAACAACGCTAGAGTGAGAAAAGATATTGTTCTG
AAGTTTCGTGATGCTGATACCCTTGAGTCCTGGTCCCAAACCATCCAAGAGTGCTTAAAT
GGTGCAGGTATGTGTGTAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
GAGAGAGAGAGAGAGATTATGAATGGTAGATCGGTTTGTAATCTCAATCCTATTAAGGAG
ATGTTTGTTTGAACATCAGCAAATTTGGCATGTAGAGGGGCAGTACACCATGCATACAAA
AGAGCGACCGAAACGATTGCTTATTTTCTTAAATCCTTTTGGAGGGAAGAAGCTGGCAAA
GGAGCTTTTTCAACAACAGATACTCAACACCAAAAT
■■Homology search results ■■ -
sp_hit_id Q8TCT0
Definition sp|Q8TCT0|CERK1_HUMAN Ceramide kinase OS=Homo sapiens
Align length 26
Score (bit) 37.4
E-value 0.065
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK963104|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0015_K05, 5'
         (636 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8TCT0|CERK1_HUMAN Ceramide kinase OS=Homo sapiens GN=CERK PE...    37   0.065
sp|Q8K4Q7|CERK1_MOUSE Ceramide kinase OS=Mus musculus GN=Cerk PE...    37   0.11 
sp|Q9NRA0|SPHK2_HUMAN Sphingosine kinase 2 OS=Homo sapiens GN=SP...    34   0.72 
sp|Q8CI15|SPHK1_MOUSE Sphingosine kinase 1 OS=Mus musculus GN=Sp...    32   2.1  
sp|Q91V26|SPHK1_RAT Sphingosine kinase 1 OS=Rattus norvegicus GN...    32   2.7  
sp|Q9NYA1|SPHK1_HUMAN Sphingosine kinase 1 OS=Homo sapiens GN=SP...    30   8.0  

>sp|Q8TCT0|CERK1_HUMAN Ceramide kinase OS=Homo sapiens GN=CERK PE=1
           SV=1
          Length = 537

 Score = 37.4 bits (85), Expect = 0.065
 Identities = 13/26 (50%), Positives = 21/26 (80%)
 Frame = +2

Query: 545 RPKRLLIFLNPFGGKKLAKELFQQQI 622
           RPK LL+F+NPFGGK   K ++++++
Sbjct: 129 RPKHLLVFINPFGGKGQGKRIYERKV 154


>sp|Q8K4Q7|CERK1_MOUSE Ceramide kinase OS=Mus musculus GN=Cerk PE=2
           SV=1
          Length = 531

 Score = 36.6 bits (83), Expect = 0.11
 Identities = 13/26 (50%), Positives = 20/26 (76%)
 Frame = +2

Query: 545 RPKRLLIFLNPFGGKKLAKELFQQQI 622
           RPK LL+F+NPFGGK   K ++++ +
Sbjct: 129 RPKHLLVFINPFGGKGQGKRIYEKTV 154


>sp|Q9NRA0|SPHK2_HUMAN Sphingosine kinase 2 OS=Homo sapiens GN=SPHK2
           PE=1 SV=2
          Length = 654

 Score = 33.9 bits (76), Expect = 0.72
 Identities = 15/37 (40%), Positives = 23/37 (62%)
 Frame = +2

Query: 515 EGQYTMHTKERPKRLLIFLNPFGGKKLAKELFQQQIL 625
           +G+ T     RP RLL+ +NPFGG+ LA +  +  +L
Sbjct: 169 DGEITPDLLPRPPRLLLLVNPFGGRGLAWQWCKNHVL 205


>sp|Q8CI15|SPHK1_MOUSE Sphingosine kinase 1 OS=Mus musculus GN=Sphk1
           PE=1 SV=1
          Length = 382

 Score = 32.3 bits (72), Expect = 2.1
 Identities = 14/26 (53%), Positives = 19/26 (73%)
 Frame = +2

Query: 545 RPKRLLIFLNPFGGKKLAKELFQQQI 622
           RP R+L+ LNP GGK  A +LFQ ++
Sbjct: 13  RPCRVLVLLNPQGGKGKALQLFQSRV 38


>sp|Q91V26|SPHK1_RAT Sphingosine kinase 1 OS=Rattus norvegicus
           GN=Sphk1 PE=1 SV=1
          Length = 383

 Score = 32.0 bits (71), Expect = 2.7
 Identities = 14/26 (53%), Positives = 19/26 (73%)
 Frame = +2

Query: 545 RPKRLLIFLNPFGGKKLAKELFQQQI 622
           RP R+L+ LNP GGK  A +LFQ ++
Sbjct: 13  RPCRVLVLLNPRGGKGKALKLFQSRV 38


>sp|Q9NYA1|SPHK1_HUMAN Sphingosine kinase 1 OS=Homo sapiens GN=SPHK1
           PE=1 SV=1
          Length = 384

 Score = 30.4 bits (67), Expect = 8.0
 Identities = 13/26 (50%), Positives = 18/26 (69%)
 Frame = +2

Query: 545 RPKRLLIFLNPFGGKKLAKELFQQQI 622
           RP R+L+ LNP GGK  A +LF+  +
Sbjct: 13  RPCRVLVLLNPRGGKGKALQLFRSHV 38
tr_hit_id A9RQ09
Definition tr|A9RQ09|A9RQ09_PHYPA Predicted protein OS=Physcomitrella patens subsp. patens
Align length 105
Score (bit) 57.8
E-value 6.0e-07
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK963104|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0015_K05, 5'
         (636 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|A9RQ09|A9RQ09_PHYPA Predicted protein OS=Physcomitrella paten...    58   6e-07
tr|Q8L7L1|Q8L7L1_ARATH Putative uncharacterized protein At4g2154...    47   0.001
tr|Q2V3G4|Q2V3G4_ARATH Uncharacterized protein At4g21540.3 OS=Ar...    47   0.001
tr|O65419|O65419_ARATH Putative uncharacterized protein F18E5.16...    47   0.001
tr|A5ALB4|A5ALB4_VITVI Putative uncharacterized protein OS=Vitis...    44   0.008
tr|A1L1R3|A1L1R3_DANRE Zgc:158263 OS=Danio rerio GN=zgc:158263 P...    42   0.024
tr|B6N5Q5|B6N5Q5_BRAFL Putative uncharacterized protein OS=Branc...    41   0.053
tr|B6LGA5|B6LGA5_BRAFL Putative uncharacterized protein (Fragmen...    41   0.053
tr|A8QCT8|A8QCT8_BRUMA Ceramide kinase, putative OS=Brugia malay...    41   0.069
tr|Q5TU47|Q5TU47_ANOGA AGAP002932-PA (Fragment) OS=Anopheles gam...    40   0.12 
tr|Q17CH0|Q17CH0_AEDAE Ceramide kinase (Fragment) OS=Aedes aegyp...    40   0.15 
tr|B0W8N4|B0W8N4_CULQU Ceramide kinase OS=Culex quinquefasciatus...    40   0.15 
tr|Q5ZAU4|Q5ZAU4_ORYSJ Putative sphingosine kinase OS=Oryza sati...    39   0.26 
tr|Q0JIR8|Q0JIR8_ORYSJ cDNA clone:002-121-E02, full insert seque...    39   0.26 
tr|B8AAF6|B8AAF6_ORYSI Putative uncharacterized protein OS=Oryza...    39   0.26 
tr|A7QYH0|A7QYH0_VITVI Chromosome undetermined scaffold_247, who...    39   0.26 
tr|A2ZYG4|A2ZYG4_ORYSJ Putative uncharacterized protein OS=Oryza...    39   0.26 
tr|Q5F3H3|Q5F3H3_CHICK Putative uncharacterized protein OS=Gallu...    39   0.34 
tr|A8XGF4|A8XGF4_CAEBR Putative uncharacterized protein OS=Caeno...    39   0.34 
tr|Q6GLV1|Q6GLV1_XENLA MGC84197 protein OS=Xenopus laevis GN=MGC...    38   0.45 
tr|A7RK69|A7RK69_NEMVE Predicted protein (Fragment) OS=Nematoste...    38   0.45 
tr|Q8H350|Q8H350_ORYSJ Putative sphingosine kinase OS=Oryza sati...    38   0.59 
tr|Q0D5G5|Q0D5G5_ORYSJ Os07g0563000 protein OS=Oryza sativa subs...    38   0.59 
tr|A3BL62|A3BL62_ORYSJ Putative uncharacterized protein OS=Oryza...    38   0.59 
tr|Q9TZI1|Q9TZI1_CAEEL Putative uncharacterized protein OS=Caeno...    38   0.59 
tr|B7PYF2|B7PYF2_IXOSC Ceramide kinase, putative (Fragment) OS=I...    38   0.59 
tr|Q4RVY2|Q4RVY2_TETNG Chromosome 9 SCAF14991, whole genome shot...    37   0.76 
tr|Q6GMF3|Q6GMF3_XENLA MGC81777 protein OS=Xenopus laevis GN=cer...    37   1.00 
tr|Q4SDW8|Q4SDW8_TETNG Chromosome undetermined SCAF14629, whole ...    37   1.00 
tr|Q9VNA6|Q9VNA6_DROME CG16708, isoform A OS=Drosophila melanoga...    37   1.00 

>tr|A9RQ09|A9RQ09_PHYPA Predicted protein OS=Physcomitrella patens
           subsp. patens GN=PHYPADRAFT_69008 PE=4 SV=1
          Length = 203

 Score = 57.8 bits (138), Expect = 6e-07
 Identities = 36/105 (34%), Positives = 57/105 (54%), Gaps = 3/105 (2%)
 Frame = +1

Query: 67  DAVPSEDMELDGRIVKATL-SRTGVIRWTSSSFSDSLVLQDDLLGFSRDHSSLTLFTFKV 243
           + V +E + LDG+ V  TL S  GV++W        L+LQ+DL+GF    S + L+TF++
Sbjct: 76  EEVLNEQVVLDGKRVLVTLDSAAGVLQWRGER-DGQLILQNDLIGFCSSESGICLYTFRM 134

Query: 244 GQVG--CCALNNARVRKDIVLKFRDADTLESWSQTIQECLNGAGM 372
            +    C      R RKD+V++F +      W  ++Q  L+ AGM
Sbjct: 135 TKSSSYCGKGLPGRKRKDMVVEFSNDGARRLWCDSLQRILDKAGM 179


>tr|Q8L7L1|Q8L7L1_ARATH Putative uncharacterized protein At4g21540
           (At4g21540) OS=Arabidopsis thaliana GN=At4g21540 PE=2
           SV=1
          Length = 485

 Score = 47.0 bits (110), Expect = 0.001
 Identities = 18/44 (40%), Positives = 33/44 (75%)
 Frame = +2

Query: 491 EHQQIWHVEGQYTMHTKERPKRLLIFLNPFGGKKLAKELFQQQI 622
           E +++W  +    + +  RPK+LL+F+NPFGGKK A+++FQ+++
Sbjct: 99  ESRKLWSDKFHQHLVSLGRPKKLLVFVNPFGGKKTARKIFQEEV 142


>tr|Q2V3G4|Q2V3G4_ARATH Uncharacterized protein At4g21540.3
           OS=Arabidopsis thaliana GN=At4g21540 PE=4 SV=1
          Length = 320

 Score = 47.0 bits (110), Expect = 0.001
 Identities = 18/44 (40%), Positives = 33/44 (75%)
 Frame = +2

Query: 491 EHQQIWHVEGQYTMHTKERPKRLLIFLNPFGGKKLAKELFQQQI 622
           E +++W  +    + +  RPK+LL+F+NPFGGKK A+++FQ+++
Sbjct: 99  ESRKLWSDKFHQHLVSLGRPKKLLVFVNPFGGKKTARKIFQEEV 142


>tr|O65419|O65419_ARATH Putative uncharacterized protein F18E5.160
            OS=Arabidopsis thaliana GN=F18E5.160 PE=4 SV=1
          Length = 1240

 Score = 47.0 bits (110), Expect = 0.001
 Identities = 18/44 (40%), Positives = 33/44 (75%)
 Frame = +2

Query: 491  EHQQIWHVEGQYTMHTKERPKRLLIFLNPFGGKKLAKELFQQQI 622
            E +++W  +    + +  RPK+LL+F+NPFGGKK A+++FQ+++
Sbjct: 888  ESRKLWSDKFHQHLVSLGRPKKLLVFVNPFGGKKTARKIFQEEV 931



 Score = 44.3 bits (103), Expect = 0.006
 Identities = 19/42 (45%), Positives = 30/42 (71%)
 Frame = +2

Query: 497 QQIWHVEGQYTMHTKERPKRLLIFLNPFGGKKLAKELFQQQI 622
           Q  W  + +  + +  RPKRLL+F+NPFGGKK A+E+F +++
Sbjct: 360 QNGWCYKLRQYLDSLGRPKRLLVFVNPFGGKKSAREIFVKEV 401



 Score = 37.4 bits (85), Expect = 0.77
 Identities = 24/95 (25%), Positives = 40/95 (42%), Gaps = 3/95 (3%)
 Frame = +1

Query: 94  LDGRIVKATLSRTGVIRWTSSSFSDSLVLQDDLLGFSRDHSSL---TLFTFKVGQVGCCA 264
           +DG +   TL+  G +RWT       L ++ D+LGF  +   +    +   + G + C  
Sbjct: 283 IDGELAMLTLTADGELRWTEYGLRQYLSMKKDVLGFIVEGKQIRVKAVVEKEAGGICCGQ 342

Query: 265 LNNARVRKDIVLKFRDADTLESWSQTIQECLNGAG 369
                VRKD V  F        W   +++ L+  G
Sbjct: 343 FGGDFVRKDFV--FEPLIDQNGWCYKLRQYLDSLG 375


>tr|A5ALB4|A5ALB4_VITVI Putative uncharacterized protein OS=Vitis
           vinifera GN=VITISV_004834 PE=4 SV=1
          Length = 487

 Score = 43.9 bits (102), Expect = 0.008
 Identities = 24/99 (24%), Positives = 45/99 (45%), Gaps = 2/99 (2%)
 Frame = +1

Query: 79  SEDMELDGRIVKATLSRTGVIRWTSSSFSDSLVLQDDLLGFSRDHSSLTLFTFKVGQVGC 258
           S+   ++G +        G IRW+       L ++ ++LGF+ + S + +        G 
Sbjct: 11  SDRFLINGTLTSVDFRDDGKIRWSDGHTDRCLAVEKEVLGFASEGSRIRIRAIVENGGGL 70

Query: 259 CALNNAR--VRKDIVLKFRDADTLESWSQTIQECLNGAG 369
           C + +    VRKD V +   AD+   W Q ++E ++  G
Sbjct: 71  CCVGSRGKLVRKDFVFEIASADSHRLWCQKLREYIDSLG 109



 Score = 38.9 bits (89), Expect = 0.26
 Identities = 16/40 (40%), Positives = 27/40 (67%)
 Frame = +2

Query: 491 EHQQIWHVEGQYTMHTKERPKRLLIFLNPFGGKKLAKELF 610
           +  ++W  + +  + +  RPKRL +F+NPFGGKK A ++F
Sbjct: 92  DSHRLWCQKLREYIDSLGRPKRLFVFVNPFGGKKSASKIF 131


>tr|A1L1R3|A1L1R3_DANRE Zgc:158263 OS=Danio rerio GN=zgc:158263 PE=2
           SV=1
          Length = 574

 Score = 42.4 bits (98), Expect = 0.024
 Identities = 16/30 (53%), Positives = 22/30 (73%)
 Frame = +2

Query: 533 HTKERPKRLLIFLNPFGGKKLAKELFQQQI 622
           H+  RP RLL+F+NPFGGKK  K++F   +
Sbjct: 122 HSPSRPHRLLVFINPFGGKKRGKQIFHSLV 151


>tr|B6N5Q5|B6N5Q5_BRAFL Putative uncharacterized protein
           OS=Branchiostoma floridae GN=BRAFLDRAFT_91023 PE=4 SV=1
          Length = 478

 Score = 41.2 bits (95), Expect = 0.053
 Identities = 15/26 (57%), Positives = 23/26 (88%)
 Frame = +2

Query: 545 RPKRLLIFLNPFGGKKLAKELFQQQI 622
           RP+RLL+F+NPFGGKK   +++QQ++
Sbjct: 126 RPRRLLVFVNPFGGKKRGVQIYQQKV 151


>tr|B6LGA5|B6LGA5_BRAFL Putative uncharacterized protein (Fragment)
           OS=Branchiostoma floridae GN=BRAFLDRAFT_164365 PE=4 SV=1
          Length = 227

 Score = 41.2 bits (95), Expect = 0.053
 Identities = 15/26 (57%), Positives = 23/26 (88%)
 Frame = +2

Query: 545 RPKRLLIFLNPFGGKKLAKELFQQQI 622
           RP+RLL+F+NPFGGKK   +++QQ++
Sbjct: 46  RPRRLLVFVNPFGGKKRGVQIYQQKV 71


>tr|A8QCT8|A8QCT8_BRUMA Ceramide kinase, putative OS=Brugia malayi
           GN=Bm1_50005 PE=4 SV=1
          Length = 551

 Score = 40.8 bits (94), Expect = 0.069
 Identities = 16/28 (57%), Positives = 24/28 (85%)
 Frame = +2

Query: 539 KERPKRLLIFLNPFGGKKLAKELFQQQI 622
           ++RPK LLIF+NPFGGK  AK+++ +Q+
Sbjct: 141 EQRPKTLLIFVNPFGGKGKAKKIYSKQV 168


>tr|Q5TU47|Q5TU47_ANOGA AGAP002932-PA (Fragment) OS=Anopheles
           gambiae GN=AGAP002932 PE=4 SV=3
          Length = 443

 Score = 40.0 bits (92), Expect = 0.12
 Identities = 16/39 (41%), Positives = 26/39 (66%)
 Frame = +2

Query: 500 QIWHVEGQYTMHTKERPKRLLIFLNPFGGKKLAKELFQQ 616
           ++W+      +  + RPK LL+FLNP+GGKK A  L+++
Sbjct: 34  ELWYNRLSSDLRDQNRPKHLLLFLNPYGGKKNALALYER 72