DK963120
Clone id TST39A01NGRL0015_K22
Library
Length 583
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0015_K22. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
GCGAGGATGAAGGCGGGCGCCGTTCTACAAAGCAAGGCGCCATGAATGCTCGGGGCTTGT
TGCGTAGTTCAGGACGAGCCCTGTAGCCCTACTGTGCAAGGCATCGAATCATCGATTCGT
TTTTTGCTGTGCATCCCGAGGATGAAGGCGGCCTTGATTAACGATGATGCAGAAGGGCTG
CGATGTCTGCTTGCTGGGGATGCTCTGTGCGCATTGAATGCCTCATGGGAGAGTCCTTTT
CAACGCGTACAGCTTTTTGCGCCTACTGTGTTGCTCACAACCTACTGAGAACTTGCCAAT
TGGTGCTGCGAACAGTAGTGTGTGCTTGTATAAGAGGAGGTGGTCGCCTCTTTCTCTCTC
CATCTAAGTCTGCCTCTTTGTCTCCATTTGAATGCGTGAGAGCATCTTGAGTGTCACTGT
TGATGGCTTGCAACCGGGACAATTGTTGTTCGTGTGCTGGCAAAGCGGAAGAATTGTGCA
TCAGCAATGGTGATTCTCTCGGAGCTGAGAGCATGGACTTCGAAGGGGGTGAGCTCCATT
GAAGGTTTTCTTTTATTTGATGCTTTGTAAACCTATTCTGGCG
■■Homology search results ■■ -
sp_hit_id Q8CIN9
Definition sp|Q8CIN9|RFFL_RAT E3 ubiquitin-protein ligase rififylin OS=Rattus norvegicus
Align length 65
Score (bit) 31.6
E-value 3.0
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK963120|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0015_K22, 5'
(583 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|Q8CIN9|RFFL_RAT E3 ubiquitin-protein ligase rififylin OS=Ratt... 32 3.0
sp|Q37871|NINE_BP82 Protein ninE OS=Enterobacteria phage 82 GN=n... 31 3.9
sp|P58069|RASA2_MOUSE Ras GTPase-activating protein 2 OS=Mus mus... 31 5.2
sp|Q47270|NINE_ECOLI Protein ninE homolog from lambdoid prophage... 30 6.7
sp|Q63713|RASA2_RAT Ras GTPase-activating protein 2 OS=Rattus no... 30 6.7
sp|Q6AJY9|PNP_DESPS Polyribonucleotide nucleotidyltransferase OS... 30 8.7
sp|Q93Z92|RING4_ARATH E3 ubiquitin-protein ligase At4g11680 OS=A... 30 8.8

>sp|Q8CIN9|RFFL_RAT E3 ubiquitin-protein ligase rififylin OS=Rattus
norvegicus GN=Rffl PE=1 SV=1
Length = 362

Score = 31.6 bits (70), Expect = 3.0
Identities = 19/65 (29%), Positives = 33/65 (50%)
Frame = -1

Query: 529 PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSNGDKEADLDGERK 350
P ++ L+ P+ S + S LP+ Q++ + A + +TQ A+ H + D E + E
Sbjct: 156 PEQQAFLTQPQSSTV-PPTSPGLPSSPAQVTSVLAQDQETQQAIGHVSQDHEEPIFLEST 214

Query: 349 RRPPP 335
R PP
Sbjct: 215 ARVPP 219


>sp|Q37871|NINE_BP82 Protein ninE OS=Enterobacteria phage 82 GN=ninE
PE=3 SV=1
Length = 56

Score = 31.2 bits (69), Expect = 3.9
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = -3

Query: 572 VYKASNKRKPSMELTPFEVHALSSERITIADAQFFRFASTR 450
+YK N+RK EL P E+ L ++ D ++ R A+ R
Sbjct: 13 IYKVPNRRKRKPELKPSEIPTLLGYTASLVDKKWLRLAARR 53


>sp|P58069|RASA2_MOUSE Ras GTPase-activating protein 2 OS=Mus
musculus GN=Rasa2 PE=1 SV=1
Length = 848

Score = 30.8 bits (68), Expect = 5.2
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = -2

Query: 462 CQHTNNNCPGCKPSTVTLKMLSRIQMETKRQT 367
CQ T+ + PGCKP T + +I ++ R+T
Sbjct: 727 CQETSESTPGCKPCTAGIPADIQIDIDEDRET 758


>sp|Q47270|NINE_ECOLI Protein ninE homolog from lambdoid prophage
DLP12 OS=Escherichia coli (strain K12) GN=ninE PE=3 SV=1
Length = 56

Score = 30.4 bits (67), Expect = 6.7
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = -3

Query: 572 VYKASNKRKPSMELTPFEVHALSSERITIADAQFFRFASTRT 447
+Y+ N+RK EL P E+ L ++ D ++ R A+ R+
Sbjct: 13 IYRVPNRRKRKPELKPSEIPTLLGYTASLVDKKWLRLAARRS 54


>sp|Q63713|RASA2_RAT Ras GTPase-activating protein 2 OS=Rattus
norvegicus GN=Rasa2 PE=2 SV=1
Length = 847

Score = 30.4 bits (67), Expect = 6.7
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = -2

Query: 462 CQHTNNNCPGCKPSTVTLKMLSRIQMETKRQT 367
CQ T+ PGCKP T + +I ++ R+T
Sbjct: 726 CQETSEGTPGCKPCTAGIPADIQIDIDEDRET 757


>sp|Q6AJY9|PNP_DESPS Polyribonucleotide nucleotidyltransferase
OS=Desulfotalea psychrophila GN=pnp PE=3 SV=1
Length = 692

Score = 30.0 bits (66), Expect = 8.7
Identities = 21/62 (33%), Positives = 30/62 (48%)
Frame = +3

Query: 339 GGRLFLSPSKSASLSPFECVRAS*VSLLMACNRDNCCSCAGKAEELCISNGDSLGAESMD 518
GGR+ K F + +S + L++AC R+ GKA+EL S + LGA
Sbjct: 150 GGRIAYIDGKYVLNPTFAELESSSMDLMVACTRNAIVMVEGKADEL--SEDEILGAIFFA 207

Query: 519 FE 524
FE
Sbjct: 208 FE 209


>sp|Q93Z92|RING4_ARATH E3 ubiquitin-protein ligase At4g11680
OS=Arabidopsis thaliana GN=At4g11680 PE=2 SV=1
Length = 390

Score = 30.0 bits (66), Expect = 8.8
Identities = 14/30 (46%), Positives = 17/30 (56%), Gaps = 3/30 (10%)
Frame = -2

Query: 507 QLRE---NHHC*CTILPLCQHTNNNCPGCK 427
+LRE NHH CT + H N+ CP CK
Sbjct: 350 ELRELPCNHHFHCTCIDKWLHINSRCPLCK 379


tr_hit_id B0CPT8
Definition tr|B0CPT8|B0CPT8_LACBS Predicted protein OS=Laccaria bicolor (strain S238N-H82)
Align length 120
Score (bit) 40.4
E-value 0.073
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK963120|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0015_K22, 5'
(583 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|B0CPT8|B0CPT8_LACBS Predicted protein OS=Laccaria bicolor (st... 40 0.073
tr|B0CW92|B0CW92_LACBS Predicted protein OS=Laccaria bicolor (st... 37 0.62
tr|Q4E5A5|Q4E5A5_TRYCR Putative uncharacterized protein OS=Trypa... 37 1.1
tr|A2DSH1|A2DSH1_TRIVA Putative uncharacterized protein OS=Trich... 36 1.4
tr|Q381C0|Q381C0_9TRYP Putative uncharacterized protein OS=Trypa... 35 2.4
tr|Q07Q99|Q07Q99_RHOP5 Methyl-accepting chemotaxis sensory trans... 35 4.0
tr|O17969|O17969_CAEEL Protein M02G9.2, partially confirmed by t... 34 5.2
tr|Q8LIH0|Q8LIH0_ORYSJ Centromere protein-like (Putative unchara... 34 5.2
tr|Q0D3H6|Q0D3H6_ORYSJ Os07g0686400 protein OS=Oryza sativa subs... 34 5.2
tr|B8M6I9|B8M6I9_9EURO Putative uncharacterized protein OS=Talar... 34 5.2
tr|A4HBI2|A4HBI2_LEIBR Putative uncharacterized protein OS=Leish... 34 6.9
tr|B6QUH3|B6QUH3_PENMA BZIP transcription factor, putative OS=Pe... 34 6.9
tr|A2R8I4|A2R8I4_ASPNC Contig An16c0230, complete genome OS=Aspe... 34 6.9
tr|Q10DW0|Q10DW0_ORYSJ Os03g0717900 protein OS=Oryza sativa subs... 33 9.0
tr|A3AM41|A3AM41_ORYSJ Putative uncharacterized protein OS=Oryza... 33 9.0
tr|A2XLF0|A2XLF0_ORYSI Putative uncharacterized protein OS=Oryza... 33 9.0

>tr|B0CPT8|B0CPT8_LACBS Predicted protein OS=Laccaria bicolor
(strain S238N-H82) GN=LACBIDRAFT_186956 PE=4 SV=1
Length = 940

Score = 40.4 bits (93), Expect = 0.073
Identities = 29/120 (24%), Positives = 54/120 (45%), Gaps = 4/120 (3%)
Frame = -1

Query: 565 KHQIKENLQWSSPPSKSMLSAPRESPLLMHNSSALPAHEQQLS----RLQAINSDTQDAL 398
+H + LQ + ++ S + LL + +P ++S RL + DT+D
Sbjct: 557 RHVLDSELQQRACEFFALASRSDDDELLQNICEEMPPFPPRVSALLGRLNRKHGDTEDKR 616

Query: 397 THSNGDKEADLDGERKRRPPPLIQAHTTVRSTNWQVLSRL*ATQ*AQKAVRVEKDSPMRH 218
T +G KEA+LD E +R L++ + T S N ++ L + +++ SP +
Sbjct: 617 TWIHGGKEANLDREATKRKTFLVENNGTASSDNQGIMDSLAGLDMSSPSIQAIGSSPQEY 676


>tr|B0CW92|B0CW92_LACBS Predicted protein OS=Laccaria bicolor
(strain S238N-H82) GN=LACBIDRAFT_322427 PE=4 SV=1
Length = 194

Score = 37.4 bits (85), Expect = 0.62
Identities = 33/102 (32%), Positives = 41/102 (40%), Gaps = 4/102 (3%)
Frame = -2

Query: 552 KKTFNGAH---PLRSPCSQLRENHHC*C-TILPLCQHTNNNCPGCKPSTVTLKMLSRIQM 385
K+ N AH PLR SQ HH C T + NC GC L L R
Sbjct: 99 KQLVNNAHRRNPLRHKASQAPLPHHAMCETSMNRSDRRERNCCGC----ARLLGLPR--- 151

Query: 384 ETKRQT*MERERGDHLLLYKHTLLFAAPIGKFSVGCEQHSRR 259
+ RQT + E G H+L + ++P G S G H R
Sbjct: 152 -SPRQTLVNPEEGSHILWRREHYAGSSPFGLTSSGHRTHFTR 192


>tr|Q4E5A5|Q4E5A5_TRYCR Putative uncharacterized protein
OS=Trypanosoma cruzi GN=Tc00.1047053506559.250 PE=4 SV=1
Length = 1559

Score = 36.6 bits (83), Expect = 1.1
Identities = 24/66 (36%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
Frame = -1

Query: 529 PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINS-DTQDALTHSNGDKEADLDGER 353
PPS S+ S PR SP L +SS+ PA ++ LQAI++ D+ D GD + E
Sbjct: 1179 PPSSSLASPPRSSPSLFSSSSS-PATDKHRRVLQAISTVDSSDVAALEKGDDSSPQAQEE 1237

Query: 352 KRRPPP 335
P
Sbjct: 1238 TAEQEP 1243


>tr|A2DSH1|A2DSH1_TRIVA Putative uncharacterized protein
OS=Trichomonas vaginalis G3 GN=TVAG_067550 PE=4 SV=1
Length = 398

Score = 36.2 bits (82), Expect = 1.4
Identities = 24/74 (32%), Positives = 37/74 (50%)
Frame = -1

Query: 529 PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSNGDKEADLDGERK 350
P S S S+PR+S H SSA P++ + L N D D + S+ DK G K
Sbjct: 74 PSSNSTPSSPRKSSA-KHRSSARPSNAKPLISDSKDNDDESD--SESSEDKSRPKTGGGK 130

Query: 349 RRPPPLIQAHTTVR 308
R+P +++ T ++
Sbjct: 131 RKPKKIVKKTTVIK 144


>tr|Q381C0|Q381C0_9TRYP Putative uncharacterized protein
OS=Trypanosoma brucei GN=Tb11.01.8390 PE=4 SV=1
Length = 2135

Score = 35.4 bits (80), Expect = 2.4
Identities = 19/53 (35%), Positives = 29/53 (54%)
Frame = -1

Query: 514 MLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSNGDKEADLDGE 356
+LS P SP L+ N LPA SRL ++ +A + ++GD E + +GE
Sbjct: 1038 ILSNPASSPQLLLNMCKLPAPAYMGSRLPLLSMPVHNARSDTSGDVEGETNGE 1090


>tr|Q07Q99|Q07Q99_RHOP5 Methyl-accepting chemotaxis sensory
transducer OS=Rhodopseudomonas palustris (strain BisA53)
GN=RPE_1941 PE=3 SV=1
Length = 568

Score = 34.7 bits (78), Expect = 4.0
Identities = 15/33 (45%), Positives = 23/33 (69%)
Frame = -1

Query: 529 PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRL 431
PP + ++ A E+ L +HN S LPAH Q+L++L
Sbjct: 63 PPPEYVIEAFLEATLALHNPSNLPAHRQRLAKL 95


>tr|O17969|O17969_CAEEL Protein M02G9.2, partially confirmed by
transcript evidence OS=Caenorhabditis elegans GN=M02G9.2
PE=2 SV=3
Length = 398

Score = 34.3 bits (77), Expect = 5.2
Identities = 23/65 (35%), Positives = 27/65 (41%), Gaps = 6/65 (9%)
Frame = +3

Query: 321 CACIRGGGR-LFLSPSKSASLSPFECVRAS*VSLLMACNRDN----CCS-CAGKAEELCI 482
C C G R + PS A P+ CV S L +CNR N C S C+ E C
Sbjct: 65 CVCTEGNVRNIITGPSLPALFKPYNCVSTCESSCLESCNRLNFNMKCSSVCSEACEFTCA 124

Query: 483 SNGDS 497
G S
Sbjct: 125 KQGTS 129


>tr|Q8LIH0|Q8LIH0_ORYSJ Centromere protein-like (Putative
uncharacterized protein) OS=Oryza sativa subsp. japonica
GN=OJ1200_C08.114 PE=4 SV=1
Length = 860

Score = 34.3 bits (77), Expect = 5.2
Identities = 21/80 (26%), Positives = 41/80 (51%), Gaps = 5/80 (6%)
Frame = -1

Query: 559 QIKENLQWSSPPSKSML-----SAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALT 395
Q++ ++ +SSP S L S + P + N S P H + +RL + + +
Sbjct: 73 QLRRSMSFSSPAGNSFLKDRSFSFSGDVPSSLFNESDAPDHVAKSTRLTNLLNKNEVLDR 132

Query: 394 HSNGDKEADLDGERKRRPPP 335
+ +G++EA + GER+++ P
Sbjct: 133 YIDGEQEATILGERQKQNSP 152


>tr|Q0D3H6|Q0D3H6_ORYSJ Os07g0686400 protein OS=Oryza sativa subsp.
japonica GN=Os07g0686400 PE=4 SV=1
Length = 926

Score = 34.3 bits (77), Expect = 5.2
Identities = 21/80 (26%), Positives = 41/80 (51%), Gaps = 5/80 (6%)
Frame = -1

Query: 559 QIKENLQWSSPPSKSML-----SAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALT 395
Q++ ++ +SSP S L S + P + N S P H + +RL + + +
Sbjct: 139 QLRRSMSFSSPAGNSFLKDRSFSFSGDVPSSLFNESDAPDHVAKSTRLTNLLNKNEVLDR 198

Query: 394 HSNGDKEADLDGERKRRPPP 335
+ +G++EA + GER+++ P
Sbjct: 199 YIDGEQEATILGERQKQNSP 218


>tr|B8M6I9|B8M6I9_9EURO Putative uncharacterized protein
OS=Talaromyces stipitatus ATCC 10500 GN=TSTA_027460 PE=4
SV=1
Length = 266

Score = 34.3 bits (77), Expect = 5.2
Identities = 23/70 (32%), Positives = 36/70 (51%)
Frame = -1

Query: 565 KHQIKENLQWSSPPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSN 386
K +++E+L SPP+ S L++P + ++ H AHE LSR + +D N
Sbjct: 27 KEKMEESLD--SPPTGSPLTSPIVTIVVGHERRLFAAHEDVLSRSAFFEAALKDQFLEPN 84

Query: 385 GDKEADLDGE 356
G K+ DL E
Sbjct: 85 G-KKVDLPDE 93