DK963522
Clone id TST39A01NGRL0016_M06
Library
Length 650
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0016_M06. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
GGCAACGGGCTTGCGAGCTATTGTGTAGGCGCAGCTGGTTGGGGAACGATAATATGTTGG
TGTGTATGTGATCTGTATAGAGAGAAATTAAGAGGATAGAATATAATTGGTGCAGCTATG
AAAACGTCAGCGACTGCGTCGACGAGCAGCAACATGATGAGCAGTGAGAGGAGTAGCATG
AGTAGCTGTGGAAGGGGGCTGGGGCTGGCGAGGACGGCCAAGGCCGTAGCGGAGGCGGTG
ATCGTCGCGGCTTGCTTGATGTGGGTGCTCTACTGGTTCTTCAAGCCGGAGCCGCAGGCC
GACGATACCCACCTCTCCAAGAACACCCGCTCTCGCTTCTTCCGCACTGATGGCCGGGAA
GGATTTTCCCTTATATACCAAACATTGCCTTTTGTTGTTGTGGCCATTTTGAGCTTTTTG
GTGATTGAGCTACGAGCTTTTATCAGGTCAAAAGAGTCAATGCCATCCATACAGGCACAA
CAAAAATTAAGATTTTCAAGTTTAAACGCCTACCCCTTGTGGGTGCATAGTCCTTTTGGA
GTTGTAACTATGGCTGATTTTCTCTTCATTATTGGCATACTCTTCATGGTGTTTTATCAC
TTTGGGAGAGTTACTCATCTATCGTTCCAAGAAATCGAGAATGGAACCGT
■■Homology search results ■■ -
sp_hit_id A4FBB2
Definition sp|A4FBB2|APT_SACEN Adenine phosphoribosyltransferase OS=Saccharopolyspora erythraea (strain NRRL 23338)
Align length 57
Score (bit) 32.7
E-value 1.7
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK963522|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0016_M06, 5'
(650 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|A4FBB2|APT_SACEN Adenine phosphoribosyltransferase OS=Sacchar... 33 1.7
sp|Q58286|Y876_METJA Putative ABC transporter permease protein M... 30 8.4

>sp|A4FBB2|APT_SACEN Adenine phosphoribosyltransferase
OS=Saccharopolyspora erythraea (strain NRRL 23338)
GN=apt PE=3 SV=1
Length = 182

Score = 32.7 bits (73), Expect = 1.7
Identities = 21/57 (36%), Positives = 29/57 (50%), Gaps = 7/57 (12%)
Frame = -3

Query: 372 KGKSFPAISAEEARAGVLGEV-------GIVGLRLRLEEPVEHPHQASRDDHRLRYG 223
+G F ++ EAR +LG G+VGLR +P + P A R D+RL YG
Sbjct: 57 RGHDFDVVAGVEARGFLLGAAVAQAHGTGVVGLR----KPGKLPEVAHRVDYRLEYG 109


>sp|Q58286|Y876_METJA Putative ABC transporter permease protein
MJ0876 OS=Methanocaldococcus jannaschii GN=MJ0876 PE=3
SV=1
Length = 351

Score = 30.4 bits (67), Expect = 8.4
Identities = 18/66 (27%), Positives = 33/66 (50%)
Frame = +1

Query: 394 VVVAILSFLVIELRAFIRSKESMPSIQAQQKLRFSSLNAYPLWVHSPFGVVTMADFLFII 573
+V +LS+ + LRA++ + IQ SL+ L P +T+ +FII
Sbjct: 154 IVALLLSYFFMGLRAYLIANAEELKIQEYWGFTIGSLSKITLGDVIP---MTICSIIFII 210

Query: 574 GILFMV 591
G++F++
Sbjct: 211 GVMFLI 216


tr_hit_id Q6PND7
Definition tr|Q6PND7|Q6PND7_CUCSA Ferric reductase OS=Cucumis sativus
Align length 128
Score (bit) 42.7
E-value 0.019
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK963522|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0016_M06, 5'
(650 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|Q6PND7|Q6PND7_CUCSA Ferric reductase OS=Cucumis sativus PE=2 ... 43 0.019
tr|A9S7N1|A9S7N1_PHYPA Ferric reductase-like transmembrane compo... 39 0.21
tr|A7PZM1|A7PZM1_VITVI Chromosome chr15 scaffold_40, whole genom... 39 0.21
tr|A5ATI3|A5ATI3_VITVI Putative uncharacterized protein OS=Vitis... 39 0.21
tr|Q9LMM2|Q9LMM2_ARATH F22L4.13 protein OS=Arabidopsis thaliana ... 38 0.47
tr|P92950|P92950_ARATH FRO1 protein OS=Arabidopsis thaliana GN=F... 38 0.47
tr|Q000W4|Q000W4_9ROSI Fe(III)-chelate reductase OS=Citrus junos... 38 0.61
tr|Q9FLW3|Q9FLW3_ARATH FRO2 homolog OS=Arabidopsis thaliana PE=4... 37 0.80
tr|B6AYT1|B6AYT1_9RHOB TRAP transporter, DctM-like membrane prot... 37 1.4
tr|Q8RWS6|Q8RWS6_ARATH Putative FRO2; NADPH oxidase OS=Arabidops... 37 1.4
tr|Q8W110|Q8W110_ARATH AT5g23980/MZF18_14 OS=Arabidopsis thalian... 36 1.8
tr|Q9LTA0|Q9LTA0_ARATH FRO2-like protein; NADPH oxidase-like OS=... 36 2.3
tr|A6FS35|A6FS35_9RHOB TRAP C4-dicarboxylate transport system pe... 35 3.0
tr|Q3KTM0|Q3KTM0_ARATH FRO1-like protein OS=Arabidopsis thaliana... 35 3.0
tr|B8A3B5|B8A3B5_MAIZE Putative uncharacterized protein OS=Zea m... 35 3.0
tr|Q9FLW2|Q9FLW2_ARATH FRO2 homolog OS=Arabidopsis thaliana PE=4... 35 4.0
tr|B6SW74|B6SW74_MAIZE Ferric reductase-like transmembrane compo... 35 4.0
tr|B5BMT4|B5BMT4_UREUR Putative membrane protein OS=Ureaplasma u... 34 6.7
tr|B3XUQ4|B3XUQ4_UREUR Putative membrane protein OS=Ureaplasma u... 34 6.7
tr|B3XT30|B3XT30_UREUR Putative membrane protein OS=Ureaplasma u... 34 6.7
tr|B2NGN9|B2NGN9_UREUR Putative membrane protein OS=Ureaplasma u... 34 6.7
tr|B2NEI6|B2NEI6_UREUR Putative membrane protein OS=Ureaplasma u... 34 6.7
tr|B2D9L7|B2D9L7_UREUR Putative membrane protein OS=Ureaplasma u... 34 6.7
tr|B1RY54|B1RY54_UREUR Putative membrane protein OS=Ureaplasma u... 34 6.7
tr|B5KGU9|B5KGU9_MALXI Ferric reductase OS=Malus xiaojinensis GN... 34 6.7

>tr|Q6PND7|Q6PND7_CUCSA Ferric reductase OS=Cucumis sativus PE=2
SV=1
Length = 694

Score = 42.7 bits (99), Expect = 0.019
Identities = 31/128 (24%), Positives = 55/128 (42%), Gaps = 4/128 (3%)
Frame = +1

Query: 256 LMWVLYWFFKPEPQADDTHLSKNTRSRFFRTD--GREGFSLIYQTLPFVVVAILSFLVIE 429
L W+ W P L K R + +++ G EG SL+ T P + +AIL +
Sbjct: 20 LGWIFLWVMMPTNTYRKKWLPK-VREKSYKSTYFGSEGTSLLMYTFPILFIAILGCVYNH 78

Query: 430 LRAFIRSKESMPSIQAQQKLRFSSLNAY--PLWVHSPFGVVTMADFLFIIGILFMVFYHF 603
L K S P+++ ++ + L + P V P G+V+ + + + ++ + F
Sbjct: 79 L----EKKRSDPNVKTRKGTKHHGLAMWKQPAIVKGPLGIVSWTELTLLTMFIILLVWSF 134

Query: 604 GRVTHLSF 627
H SF
Sbjct: 135 ATYLHNSF 142


>tr|A9S7N1|A9S7N1_PHYPA Ferric reductase-like transmembrane
component OS=Physcomitrella patens subsp. patens
GN=PHYPADRAFT_163308 PE=4 SV=1
Length = 801

Score = 39.3 bits (90), Expect = 0.21
Identities = 26/126 (20%), Positives = 49/126 (38%)
Frame = +1

Query: 262 WVLYWFFKPEPQADDTHLSKNTRSRFFRTDGREGFSLIYQTLPFVVVAILSFLVIELRAF 441
W WF P + + + + + P + +AI + L +EL
Sbjct: 68 WFTLWFRMPSTEGRAFRKTVQDELNYSTFWSKAATNYTMYAAPVIALAIFALLFLELEER 127

Query: 442 IRSKESMPSIQAQQKLRFSSLNAYPLWVHSPFGVVTMADFLFIIGILFMVFYHFGRVTHL 621
+ + Q + ++ P+ V +P GVVT+ D + I +L + +GR+T
Sbjct: 128 YPERRCPSAKQPMLVRLYRAIWTQPILVKTPIGVVTIIDIVIIGMVLVCTCWIWGRLTVR 187

Query: 622 SFQEIE 639
F I+
Sbjct: 188 QFAAID 193


>tr|A7PZM1|A7PZM1_VITVI Chromosome chr15 scaffold_40, whole genome
shotgun sequence OS=Vitis vinifera GN=GSVIVT00026928001
PE=4 SV=1
Length = 714

Score = 39.3 bits (90), Expect = 0.21
Identities = 28/106 (26%), Positives = 51/106 (48%)
Frame = +1

Query: 325 TRSRFFRTDGREGFSLIYQTLPFVVVAILSFLVIELRAFIRSKESMPSIQAQQKLRFSSL 504
T S +F GR+G +++ T P + A+L + L + + +S S K R + L
Sbjct: 58 TDSTYF---GRQGATILIYTFPVLFSAVLGCAYLHLGKKLNNNKSKSS----PKDRLAVL 110

Query: 505 NAYPLWVHSPFGVVTMADFLFIIGILFMVFYHFGRVTHLSFQEIEN 642
P+ V P G+V+M + LF++ + ++ + FQ IE+
Sbjct: 111 KR-PVLVKGPLGIVSMIEMLFLVMFIALLVWSIYTYLRTGFQGIED 155


>tr|A5ATI3|A5ATI3_VITVI Putative uncharacterized protein OS=Vitis
vinifera GN=VITISV_034719 PE=4 SV=1
Length = 792

Score = 39.3 bits (90), Expect = 0.21
Identities = 28/106 (26%), Positives = 51/106 (48%)
Frame = +1

Query: 325 TRSRFFRTDGREGFSLIYQTLPFVVVAILSFLVIELRAFIRSKESMPSIQAQQKLRFSSL 504
T S +F GR+G +++ T P + A+L + L + + +S S K R + L
Sbjct: 58 TDSTYF---GRQGATILIYTFPVLFSAVLGCAYLHLGKKLNNNKSKSS----PKDRLAVL 110

Query: 505 NAYPLWVHSPFGVVTMADFLFIIGILFMVFYHFGRVTHLSFQEIEN 642
P+ V P G+V+M + LF++ + ++ + FQ IE+
Sbjct: 111 KR-PVLVKGPLGIVSMIEMLFLVMFIALLVWSIYTYLRTGFQGIED 155


>tr|Q9LMM2|Q9LMM2_ARATH F22L4.13 protein OS=Arabidopsis thaliana
GN=At1g01590 PE=4 SV=1
Length = 704

Score = 38.1 bits (87), Expect = 0.47
Identities = 18/95 (18%), Positives = 48/95 (50%)
Frame = +1

Query: 352 GREGFSLIYQTLPFVVVAILSFLVIELRAFIRSKESMPSIQAQQKLRFSSLNAYPLWVHS 531
GR G +L+ P +++A L + + L+ + ++ +++ +F +L P+ V+
Sbjct: 57 GRPGVNLLVYMFPMILLAFLGCIYLHLKKSTTVNQFNSGVE-KKRAKFGALRR-PMLVNG 114

Query: 532 PFGVVTMADFLFIIGILFMVFYHFGRVTHLSFQEI 636
P G+VT+ + +F+ + ++ + + +F +
Sbjct: 115 PLGIVTVTEVMFLTMFMALLLWSLANYMYRTFVNV 149


>tr|P92950|P92950_ARATH FRO1 protein OS=Arabidopsis thaliana GN=FRO1
PE=4 SV=1
Length = 704

Score = 38.1 bits (87), Expect = 0.47
Identities = 18/95 (18%), Positives = 48/95 (50%)
Frame = +1

Query: 352 GREGFSLIYQTLPFVVVAILSFLVIELRAFIRSKESMPSIQAQQKLRFSSLNAYPLWVHS 531
GR G +L+ P +++A L + + L+ + ++ +++ +F +L P+ V+
Sbjct: 57 GRPGVNLLVYMFPMILLAFLGCIYLHLKKSTTVNQFNSGVE-KKRAKFGALRR-PMLVNG 114

Query: 532 PFGVVTMADFLFIIGILFMVFYHFGRVTHLSFQEI 636
P G+VT+ + +F+ + ++ + + +F +
Sbjct: 115 PLGIVTVTEVMFLTMFMALLLWSLANYMYRTFVNV 149


>tr|Q000W4|Q000W4_9ROSI Fe(III)-chelate reductase OS=Citrus junos
PE=2 SV=1
Length = 700

Score = 37.7 bits (86), Expect = 0.61
Identities = 30/127 (23%), Positives = 61/127 (48%), Gaps = 5/127 (3%)
Frame = +1

Query: 256 LMWVLYWFFKPEPQADDT---HLSKNTRSRFFRTDGREGFSLIYQTLPFVVVAILSFLVI 426
L W+L W P +T L+ S +FR +G +L+ + P +++A+L + +
Sbjct: 17 LGWLLVWALIPTKVYKNTWTPKLNNKLNSTYFR---EQGTNLLLFSFPVMLIALLGCVYL 73

Query: 427 ELRAFIRSKESMPSIQAQQKLRFSSL--NAYPLWVHSPFGVVTMADFLFIIGILFMVFYH 600
L+ + +Q+++K+R L + P+ V +P G+VT + I ++F+ F
Sbjct: 74 HLQ------KKPNKLQSKRKMRSPRLFFSQRPVLVMAPLGIVTAIE--LTIAVMFVAFLI 125

Query: 601 FGRVTHL 621
+ +L
Sbjct: 126 WALANYL 132


>tr|Q9FLW3|Q9FLW3_ARATH FRO2 homolog OS=Arabidopsis thaliana PE=4
SV=1
Length = 703

Score = 37.4 bits (85), Expect = 0.80
Identities = 30/132 (22%), Positives = 60/132 (45%), Gaps = 8/132 (6%)
Frame = +1

Query: 256 LMWVLYWFF--------KPEPQADDTHLSKNTRSRFFRTDGREGFSLIYQTLPFVVVAIL 411
L W+L W K P+ LSK + +F G +G +L+ T+P + +A+L
Sbjct: 17 LGWILVWIMISTNLFKSKWTPK-----LSKYLNTTYF---GPQGTNLVLLTVPMMFIAVL 68

Query: 412 SFLVIELRAFIRSKESMPSIQAQQKLRFSSLNAYPLWVHSPFGVVTMADFLFIIGILFMV 591
S + + IR+K S + ++ + + + V +P G+VT + F + + ++
Sbjct: 69 SCVYLH----IRNKLSQSYLYECREWKLKRIMGRVIMVMNPLGIVTATELTFSLLFVALL 124

Query: 592 FYHFGRVTHLSF 627
+ +LS+
Sbjct: 125 AWSLYNYLYLSY 136


>tr|B6AYT1|B6AYT1_9RHOB TRAP transporter, DctM-like membrane protein
OS=Rhodobacterales bacterium HTCC2083 GN=RB2083_345 PE=4
SV=1
Length = 784

Score = 36.6 bits (83), Expect = 1.4
Identities = 27/98 (27%), Positives = 45/98 (45%)
Frame = +1

Query: 334 RFFRTDGREGFSLIYQTLPFVVVAILSFLVIELRAFIRSKESMPSIQAQQKLRFSSLNAY 513
R R D + G +I+ TL A++ ++I + +R + PS + F AY
Sbjct: 548 RRLREDDKSGKVVIFSTL-----AVILMILIGVNFDLRVNQGSPSFETWVAFAF----AY 598

Query: 514 PLWVHSPFGVVTMADFLFIIGILFMVFYHFGRVTHLSF 627
W+++ FG++ LF GIL V +VT + F
Sbjct: 599 TAWLYAFFGLLFSCYVLFKAGILPPVVRETAKVTSMVF 636


>tr|Q8RWS6|Q8RWS6_ARATH Putative FRO2; NADPH oxidase OS=Arabidopsis
thaliana GN=At5g49730 PE=2 SV=1
Length = 738

Score = 36.6 bits (83), Expect = 1.4
Identities = 31/112 (27%), Positives = 55/112 (49%)
Frame = +1

Query: 262 WVLYWFFKPEPQADDTHLSKNTRSRFFRTDGREGFSLIYQTLPFVVVAILSFLVIELRAF 441
WV++ Q D L+ + F T G LI+ + P +V+++L+ L +
Sbjct: 39 WVVFLMMMYPEQLGDDFLTFVSSKTFLGTTGT--MFLIF-SCPILVISVLASLYL----I 91

Query: 442 IRSKESMPSIQAQQKLRFSSLNAYPLWVHSPFGVVTMADFLFIIGILFMVFY 597
I ++ + + + K L +P+ V PFGVV+ A+FL I ++F VF+
Sbjct: 92 ISGEDKVFTKKKISKFPRFRLWTFPVLVDGPFGVVSAAEFLGI--MVFSVFF 141