Name YMU02

  Sporophytes of Adiantum capillus-veneris were grown in a green house. Total RNA was prepared from the young leaves as described by Kanegae and Wada (1998). To purify poly(A)+ RNA, we used an mRNA purification kit (Amersham Pharmacia Biotech) according to the manufacturer’s instructions as described by Yamauchi et al. (2005). A cDNA library was constructed with Creator SMART cDNA library constructionkit (Clontech).

  • Kanegae, T., Wada, M. (1998) Isolation and characterization of homologues of plant blue-light photoreceptor (cryptochrome) genes from the fern Adiantum capillus-veneris. Mol. Gen. Genet. 259: 345-353. PubMed ID: 9790588

  • Yamauchi, D., Sutoh, K., Kanegae, H., Horiguchi, T., Matsuoka, K., Fukuda, H., Wada, M. (2005) Analysis of expressed sequence tags in prothallia of Adiantum capillus-veneris.  J. Plant Res. 118 (3): 223-227. PubMed ID: 15940394