Entry URI http://metadb.riken.jp/db/SciNetS_ria224i/cria224u4ria224u15295103i
Entry name Cruz-Ram鱈rez Alfredo et al. 2004 Aug. Plant Cell 16(8):2020-34.
Title The xipotl mutant of Arabidopsis reveals a critical role for phospholipid metabolism in root system development and epidermal cell integrity.
Authors Cruz-Ram鱈rez Alfredo|Gonz叩lez-Ortega Emmanuel|Herrera-Estrella Luis|L坦pez-Bucio Jos辿|Ram鱈rez-Ch叩vez Enrique|Ram鱈rez-Pimentel Gabriel|S叩nchez-Calderon Lenin|Zurita-Silva Andr辿s
Abstract Phosphocholine (PCho) is an essential metabolite for plant development because it is the precursor for the biosynthesis of phosphatidylcholine, which is the major lipid component in plant cell membranes. The main step in PCho biosynthesis in Arabidopsis thaliana is the triple, sequential N-methylation of phosphoethanolamine, catalyzed by S-adenosyl-l-methionine:phosphoethanolamine N-methyltransferase (PEAMT). In screenings performed to isolate Arabidopsis mutants with altered root system architecture, a T-DNA mutagenized line showing remarkable alterations in root development was isolated. At the seedling stage, the mutant phenotype is characterized by a short primary root, a high number of lateral roots, and short epidermal cells with aberrant morphology. Genetic and biochemical characterization of this mutant showed that the T-DNA was inserted at the At3g18000 locus (XIPOTL1), which encodes PEAMT (XIPOTL1). Further analyses revealed that inhibition of PCho biosynthesis in xpl1 mutants not only alters several root developmental traits but also induces cell death in root epidermal cells. Epidermal cell death could be reversed by phosphatidic acid treatment. Taken together, our results suggest that molecules produced downstream of the PCho biosynthesis pathway play key roles in root development and act as signals for cell integrity.
Pubmed ID 15295103
Journal The Plant cell
Volume 16
Issue 8
Pages 2020-34
Publication date 2004 Aug
Num of phenotype gene 1