Entry URI http://metadb.riken.jp/db/SciNetS_ria224i/cria224u4ria224u15772667i
Entry name Peiter Edgar et al. 2005 Mar. Nature 434(7031):404-8.
Title The vacuolar Ca2+-activated channel TPC1 regulates germination and stomatal movement.
Authors Hetherington Alistair M|Knight Heather|Maathuis Frans J M|Mills Lewis N|Peiter Edgar|Pelloux J辿r担me|Sanders Dale
Abstract Cytosolic free calcium ([Ca2+]cyt) is a ubiquitous signalling component in plant cells. Numerous stimuli trigger sustained or transient elevations of [Ca2+]cyt that evoke downstream stimulus-specific responses. Generation of [Ca2+]cyt signals is effected through stimulus-induced opening of Ca2+-permeable ion channels that catalyse a flux of Ca2+ into the cytosol from extracellular or intracellular stores. Many classes of Ca2+ current have been characterized electrophysiologically in plant membranes. However, the identity of the ion channels that underlie these currents has until now remained obscure. Here we show that the TPC1 ('two-pore channel 1') gene of Arabidopsis thaliana encodes a class of Ca2+-dependent Ca2+-release channel that is known from numerous electrophysiological studies as the slow vacuolar channel. Slow vacuolar channels are ubiquitous in plant vacuoles, where they form the dominant conductance at micromolar [Ca2+]cyt. We show that a tpc1 knockout mutant lacks functional slow vacuolar channel activity and is defective in both abscisic acid-induced repression of germination and in the response of stomata to extracellular calcium. These studies unequivocally demonstrate a critical role of intracellular Ca2+-release channels in the physiological processes of plants.
Pubmed ID 15772667
Journal Nature
Volume 434
Issue 7031
Pages 404-8
Publication date 2005 Mar
Num of phenotype gene 0