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Entry URI http://metadb.riken.jp/db/SciNetS_ria224i/cria224u4ria224u16107538i
Entry name Stevenson-Paulik Jill et al. 2005 Aug. Proc. Natl. Acad. Sci. U.S.A. 102(35):12612-7.
Title Generation of phytate-free seeds in Arabidopsis through disruption of inositol polyphosphate kinases.
Authors Bastidas Robert J|Chiou Shean-Tai|Frye Roy A|Stevenson-Paulik Jill|York John D
Abstract Phytate (inositol hexakisphosphate, IP6) is a regulator of intracellular signaling, a highly abundant animal antinutrient, and a phosphate store in plant seeds. Here, we report a requirement for inositol polyphosphate kinases, AtIPK1 and AtIPK2beta, for the later steps of phytate synthesis in Arabidopsis thaliana. Coincident disruption of these kinases nearly ablates seed phytate without accumulation of phytate precursors, increases seed-free phosphate by 10-fold, and has normal seed yield. Additionally, we find a requirement for inositol tetrakisphosphate (IP4)/inositol pentakisphosphate (IP5) 2-kinase activity in phosphate sensing and root hair elongation. Our results define a commercially viable strategy for the genetic engineering of phytate-free grain and provide insights into the role of inositol polyphosphate kinases in phosphate signaling biology.
Pubmed ID 16107538
Journal Proceedings of the National Academy of Sciences of the United States of America
Volume 102
Issue 35
Pages 12612-7
Publication date 2005 Aug
Num of phenotype gene 0