Entry URI http://metadb.riken.jp/db/SciNetS_ria224i/cria224u4ria224u12753583i
Entry name Friesner Joanna et al. 2003 May. Plant J. 34(4):427-40.
Title Ku80- and DNA ligase IV-deficient plants are sensitive to ionizing radiation and defective in T-DNA integration.
Authors Britt Anne B|Friesner Joanna
Abstract Double-strand break (DSB) repair pathways catalyze the rejoining of broken chromosomes and the integration of transforming DNAs. These processes have been well characterized in bacteria, fungi, and animals. Plants are generally thought primarily to utilize a non-homologous end joining (NHEJ) pathway to repair DSBs and integrate transgenes, as transforming DNAs with large tracts of homology to the chromosome are integrated at random. In order to test the hypothesis that NHEJ is an important pathway for the repair of DSBs in plants, we isolated T-DNA insertion mutations in the Arabidopsis homologs of the Ku80 and DNA ligase IV genes, required for the initiation and completion, respectively, of NHEJ. Both mutants were hypersensitive to the cytostatic effects of gamma radiation, suggesting that NHEJ is indeed a critical pathway for the repair of DSBs. T-DNA insertion rates were also decreased in the mutants, indicating that Ku80 and DNA ligase IV play an important role in either the mechanism or the regulation of T-DNA integration in Arabidopsis.
Pubmed ID 12753583
Journal The Plant journal
Volume 34
Issue 4
Pages 427-40
Publication date 2003 May
Num of phenotype gene 0