Entry URI http://metadb.riken.jp/db/SciNetS_ria224i/cria224u4ria224u16936718i
Entry name Jaillais Yvon et al. 2006 Sep. Nature 443(7107):106-9.
Title AtSNX1 defines an endosome for auxin-carrier trafficking in Arabidopsis.
Authors Fobis-Loisy Isabelle|Gaude Thierry|Jaillais Yvon|Miège Christine|Rollin Claire
Abstract Polarized cellular distribution of the phytohormone auxin and its carriers is essential for normal plant growth and development. Polar auxin transport is maintained by a network of auxin influx (AUX) and efflux (PIN) carriers. Both auxin transport and PIN protein cycling between the plasma membrane and endosomes require the activity of the endosomal GNOM; however, intracellular routes taken by these carriers remain largely unknown. Here we show that Arabidopsis thaliana SORTING NEXIN 1 (AtSNX1) is involved in the auxin pathway and that PIN2, but not PIN1 or AUX1, is transported through AtSNX1-containing endosomes. We demonstrate that the snx1-null mutant exhibits multiple auxin-related defects and that loss of function of AtSNX1 severely enhances the phenotype of a weak gnom mutant. In root cells, we further show that AtSNX1 localizes to an endosomal compartment distinct from GNOM-containing endosomes, and that PIN2 accumulates in this compartment after treatment with the phosphatidylinositol-3-OH kinase inhibitor wortmannin or after a gravity stimulus. Our data reveal the existence of a novel endosomal compartment involved in PIN2 endocytic sorting and plant development.
Pubmed ID 16936718
Journal Nature
Volume 443
Issue 7107
Pages 106-9
Publication date 2006 Sep
Num of phenotype gene 3