Entry URI http://metadb.riken.jp/db/SciNetS_ria224i/cria224u4ria224u17617174i
Entry name Hassidim Miriam et al. 2007 Aug. Plant J. 51(4):551-62.
Title Mutations in CHLOROPLAST RNA BINDING provide evidence for the involvement of the chloroplast in the regulation of the circadian clock in Arabidopsis.
Authors Fradkin David|Green Rachel M|Harir Yael|Hassidim Miriam|Hilman Dror|Keren Nir|Kron Ido|Yakir Esther|Yerushalmi Shai
Abstract The Arabidopsis circadian system regulates the expression of up to 36% of the nuclear genome, including many genes that encode photosynthetic proteins. The expression of nuclear-encoded photosynthesis genes is also regulated by signals from the chloroplasts, a process known as retrograde signaling. We have identified CHLOROPLAST RNA BINDING (CRB), a putative RNA-binding protein, and have shown that it is important for the proper functioning of the chloroplast. crb plants are smaller and paler than wild-type plants, and have altered chloroplast morphology and photosynthetic performance. Surprisingly, mutations in CRB also affect the circadian system, altering the expression of both oscillator and output genes. In order to determine whether the changes in circadian gene expression are specific to mutations in the CRB gene, or are more generally caused by the malfunctioning of the chloroplast, we also examined the circadian system in mutations affecting STN7, GUN1, and GUN5, unrelated nuclear-encoded chloroplast proteins known to be involved in retrograde signaling. Our results provide evidence that the functional state of the chloroplast may be an important factor that affects the circadian system.
Pubmed ID 17617174
Journal The Plant journal
Volume 51
Issue 4
Pages 551-62
Publication date 2007 Aug
Num of phenotype gene 0