Entry URI http://metadb.riken.jp/db/SciNetS_ria224i/cria224u4ria224u18003916i
Entry name Tomatsu Hajime et al. 2007 Nov. Proc. Natl. Acad. Sci. U.S.A. 104(47):18807-12.
Title An Arabidopsis thaliana high-affinity molybdate transporter required for efficient uptake of molybdate from soil.
Authors Fujiwara Toru|Shibagaki Nakako|Takahashi Hideki|Takano Junpei|Tomatsu Hajime|Watanabe-Takahashi Akiko
Abstract Molybdenum (Mo) is a trace element essential for living organisms, however no molybdate transporter has been identified in eukaryotes. Here, we report the identification of a molybdate transporter, MOT1, from Arabidopsis thaliana. MOT1 is expressed in both roots and shoots, and the MOT1 protein is localized, in part, to plasma membranes and to vesicles. MOT1 is required for efficient uptake and translocation of molybdate and for normal growth under conditions of limited molybdate supply. Kinetics studies in yeast revealed that the K(m) value of MOT1 for molybdate is approximately 20 nM. Furthermore, Mo uptake by MOT1 in yeast was not affected by coexistent sulfate, and MOT1 did not complement a sulfate transporter-deficient yeast mutant strain. These data confirmed that MOT1 is specific for molybdate and that the high affinity of MOT1 allows plants to obtain scarce Mo from soil.
Pubmed ID 18003916
Journal Proceedings of the National Academy of Sciences of the United States of America
Volume 104
Issue 47
Pages 18807-12
Publication date 2007 Nov
Num of phenotype gene 0