Entry URI http://metadb.riken.jp/db/SciNetS_ria224i/cria224u4ria224u18544632i
Entry name Riehs Nina et al. 2008 Jul. J. Cell. Sci. 121(Pt 13):2208-16.
Title Arabidopsis SMG7 protein is required for exit from meiosis.
Authors Akimcheva Svetlana|Bulankova Petra|Idol Rachel A|Puizina Jasna|Riehs Nina|Riha Karel|Schleiffer Alexander|Schweizer Dieter|Shippen Dorothy E|Siroky Jiri
Abstract Meiosis consists of two nuclear divisions that are separated by a short interkinesis. Here we show that the SMG7 protein, which plays an evolutionarily conserved role in nonsense-mediated RNA decay (NMD) in animals and yeast, is essential for the progression from anaphase to telophase in the second meiotic division in Arabidopsis. Arabidopsis SMG7 is an essential gene, the disruption of which causes embryonic lethality. Plants carrying a hypomorphic smg7 mutation exhibit an elevated level of transcripts containing premature stop codons. This suggests that the role of SMG7 in NMD is conserved in plants. Furthermore, hypomorphic smg7 alleles render mutant plants sterile by causing an unusual cell-cycle arrest in anaphase II that is characterized by delayed chromosome decondensation and aberrant rearrangement of the meiotic spindle. The smg7 phenotype was mimicked by exposing meiocytes to the proteasome inhibitor MG115. Together, these data indicate that SMG7 counteracts cyclin-dependent kinase (CDK) activity at the end of meiosis, and reveal a novel link between SMG7 and regulation of the meiotic cell cycle.
Pubmed ID 18544632
Journal Journal of cell science
Volume 121
Issue Pt 13
Pages 2208-16
Publication date 2008 Jul
Num of phenotype gene 0