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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFF358
Library CF
(Link to library)
Clone ID CFF358
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID CFF358E
(Link to Original site)
Representative DNA sequence 
>CFF358 (CFF358Q) /CSM/CF/CFF3-C/CFF358Q.Seq.d/
ATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAAAAA
AAAAAATAAAAAATGGATGGNGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCAGGT
ATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATTGTT
GGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTCGGN
GATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGTATT
GTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTTCGN
GTTGCTCCANAGGAACATCCAGTTCTNTTAACTGAAGCTTCATTAAATCCAAAAGCAAAT
AGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTTGCC
ATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGATTCA
GGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGGTTATGCATTACCACATGCTAT
TTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTAACTGA
ACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATATTAAAGAGAA
ATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCATCATCTGC
ATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAATGAACGTTT
CCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCTGGTATTCA
TGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATTTATATGG
TAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAATAA
AGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCACCACCAGAACG
NAAANACTCTGTATGG
sequence update 2001. 6. 9
Translated Amino Acid sequence 
lnnskdfnyililnk*tnkkknkkwmxkmfkl*llimvqvcvklvllvmmlhvlfshqll
vvqdtlvlwlvwvkrihmsxmklnqkevf*hlsiqlnmvlspigmiwkksgiilfimnfx
llxrniqfx*lklh*iqkqierk*LKSCLKLSTLQQCMLPFKLYYHSMPLVVPLVSLWIQ
VMVFPILYQFTKGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEK
LAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIH
ETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPER
KXSVW
Translated Amino Acid sequence (All Frames) 
Frame A:
ikqlkrf*lyfnik*ink*kkk*kmdgedvqalvidngsgmckagfagddapravfpsiv
grprhtgvmvgmgqkdsyvgdeaqskrgiltlkypiehgivtnwddmekiwhhtfynelr
vapxehpvllteaslnpkanrekmtqimfetfntpamyvaiqavlslyasgrttgivmds
gdgvshtvpiyeglcittcyftfrfsws*sy*lhdenln*twllihnnc*krnr*ry*re
isircigf*s*nancsliicireii*itrwssyynw**tfplsrstfptiilgygvcwys
*nnl*fnhem*c*ys*rfiw*cciirwynyvpryc*sye*rinctctinnen*nycttrt
xxlcm


Frame B:
lnnskdfnyililnk*tnkkknkkwmxkmfkl*llimvqvcvklvllvmmlhvlfshqll
vvqdtlvlwlvwvkrihmsxmklnqkevf*hlsiqlnmvlspigmiwkksgiilfimnfx
llxrniqfx*lklh*iqkqierk*LKSCLKLSTLQQCMLPFKLYYHSMPLVVPLVSLWIQ
VMVFPILYQFTKGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEK
LAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIH
ETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPER
KXSVW


Frame C:
*ttqkiliif*y*inkqikkkikngwxrcssfsy**wfryv*swfcw**cstccfpincw
sskthwcygwygskgficrx*ssikkryfnt*vsn*twychqlg*ygknlasyfl**tsx
csxgtsssxn*sfiksksk*rendsnhv*nfqhssnvcchssciitlclwsyhwyrygfr
*wcfpyctnlrrvmhyhmlfyv*i*lvvillit**ks*lnvvthsqqllkeksleilkrn
*htlhwilklkcklqphhlh*rnhmnyqmvkllqlvmnvsvvqkhfsnhhswvwsllvfm
kqpiiqs*nvmlifvkiymvmlyyqvvqlcsqvlliv*ikn*lhlhhqq*klkllhhqnx
xtly
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFF358 (CFF358Q) /CSM/CF/CFF3-C/CFF358Q.Seq.d/                       1986   0.0
SFL261 (SFL261Q) /CSM/SF/SFL2-C/SFL261Q.Seq.d/                       1978   0.0
SFD790 (SFD790Q) /CSM/SF/SFD7-D/SFD790Q.Seq.d/                       1978   0.0
SFD319 (SFD319Q) /CSM/SF/SFD3-A/SFD319Q.Seq.d/                       1978   0.0
CHD357 (CHD357Q) /CSM/CH/CHD3-C/CHD357Q.Seq.d/                       1978   0.0
CFI221 (CFI221Q) /CSM/CF/CFI2-A/CFI221Q.Seq.d/                       1978   0.0
SFD813 (SFD813Q) /CSM/SF/SFD8-A/SFD813Q.Seq.d/                       1970   0.0
AFE187 (AFE187Q) /CSM/AF/AFE1-D/AFE187Q.Seq.d/                       1970   0.0
AFB839 (AFB839Q) /CSM/AF/AFB8-B/AFB839Q.Seq.d/                       1970   0.0
SFG742 (SFG742Q) /CSM/SF/SFG7-B/SFG742Q.Seq.d/                       1965   0.0
own update 2002.11.18
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.    842   0.0    2
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   803   0.0    16
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   726   0.0    15
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   726   0.0    20
X03284|X03284.1 Dictyostelium discoideum gene for actin A3-S2.    724   0.0    3
X03282|X03282.1 dictyostelium discoideum gene for actin A12.      702   0.0    2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   678   0.0    14
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.         620   0.0    2
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   620   0.0    7
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   613   0.0    13
dna update 2003.12.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   344   0.0
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   344   0.0
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        342   0.0
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   342   0.0
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   342   0.0
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   342   0.0
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          336   0.0
AY294152_1(AY294152|pid:none) Mayorella sp. JJP-2003 actin mRNA,...   336   0.0
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   335   0.0
AY294153_1(AY294153|pid:none) Platyamoeba placida actin mRNA, pa...   333   0.0
protein update 2009. 6.30
PSORT 

 psg: 0.82  gvh: 0.45  alm: 0.26  top: 0.53  tms: 0.07  mit: 0.41  mip: 0.10
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.38  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 20.0 %: endoplasmic reticulum
	 16.0 %: Golgi
	 12.0 %: cytoplasmic
	 12.0 %: nuclear
	  8.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for CFF358 is mit
5' end seq. ID CFF358F
5' end seq. 
>CFF358F.Seq
ATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAAAAA
AAAAAATAAAAAATGGATGGNGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCAGGT
ATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATTGTT
GGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTCGGN
GATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGTATT
GTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTTCGN
GTTGCTCCANAGGAACATCCAGTTCTNTTAACTGAAGCTTCATTAAATCCAAAAGCAAAT
AGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTTGCC
ATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTA----------
Length of 5' end seq. 517
3' end seq. ID CFF358Z
3' end seq. 
>CFF358Z.Seq
----------CCAGCAATGTATGTTGCCATTCAAGCTGTATTATCACTCTATGCCTCTGG
TCGTACCACTGGTATCGTTATGGATTCAGGTGATGGTGTTTCCCATACTGTACCAATTTA
CGAAGGGTTATGCATTACCACATGCTATTTTACGTTTAGATTTAGCTGGTCGTGATCTTA
CTGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACAACAACTGCTGAAA
GAGAAATCGTTAGAGATATTAAAGAGAAATTAGCATACGTTGCATTGGATTTTGAAGCTG
AAATGCAAACTGCAGCCTCATCATCTGCATTAGAGAAATCATATGAATTACCAGATGGTC
AAGTTATTACAATTGGTAATGAACGTTTCCGTTGTCCAGAAGCACTTTTCCAACCATCAT
TCTTGGGTATGGAGTCTGCTGGTATTCATGAAACAACCTATAATTCAATCATGAAATGTG
ATGTTGATATTCGTAAAGATTTATATGGTAATGTTGTATTATCAGGTGGTACAACTATGT
TCCCAGGTATTGCTGATCGTATGAATAAAGAATTAACTGCACTTGCACCATCAACAATGA
AAATTAAAATTATTGCACCACCAGAACGNAAANACTCTGTATGG
Length of 3' end seq. 634
Connected seq. ID CFF358P
Connected seq. 
>CFF358P.Seq
ATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAAAAA
AAAAAATAAAAAATGGATGGNGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCAGGT
ATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATTGTT
GGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTCGGN
GATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGTATT
GTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTTCGN
GTTGCTCCANAGGAACATCCAGTTCTNTTAACTGAAGCTTCATTAAATCCAAAAGCAAAT
AGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTTGCC
ATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTA----------CCAGCAATGTATG
TTGCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGG
ATTCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGGTTATGCATTACCACAT
GCTATTTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTA
ACTGAACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATATTAAA
GAGAAATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCATCA
TCTGCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAATGAA
CGTTTCCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCTGGT
ATTCATGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATTTA
TATGGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATG
AATAAAGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCACCACCA
GAACGNAAANACTCTGTATGG
Length of connected seq. 1151
Full length Seq ID CFF358E
Full length Seq. 
>CFF358E.Seq
ATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAAAAA
AAAAAATAAAAAATGGATGGNGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCAGGT
ATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATTGTT
GGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTCGGN
GATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGTATT
GTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTTCGN
GTTGCTCCANAGGAACATCCAGTTCTNTTAACTGAAGCTTCATTAAATCCAAAAGCAAAT
AGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTTGCC
ATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGATTCA
GGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGGTTATGCATTACCACATGCTAT
TTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTAACTGA
ACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATATTAAAGAGAA
ATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCATCATCTGC
ATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAATGAACGTTT
CCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCTGGTATTCA
TGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATTTATATGG
TAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAATAA
AGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCACCACCAGAACG
NAAANACTCTGTATGG
Length of full length seq. 1096