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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHJ161
Library CH
(Link to library)
Clone ID CHJ161
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16419-1
Original site URL
Representative seq. ID CHJ161P
(Link to Original site)
Representative DNA sequence 
>CHJ161 (CHJ161Q) /CSM/CH/CHJ1-C/CHJ161Q.Seq.d/
ATTCAATTTTATTTTTAATCAATTCAAATTAAAAAAAAAACAATAACTAACTAAGTTAAA
AATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTATGATATT
CCTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAANAANAATGTCA
TAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGTGGTGAACAAAAATGGGC
TTGCTCTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATANAAATTCATC
CAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTATTAAAAT
TACAACTCCAACCGAGTATTCTTGTTCTGATGCCAANATTAAAGAATGTGCCTTATTAGG
TAAATCATGTAGTTTCCAAAANAACTCTTGTTCAAATCCAACTTCCTGTTGCCCAGGTGA
ATCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAACATCTGGTGGTGGTTCATCAGGTGG
TACATCGGGTGGTAGTTCATCAGGTGGTACATCAGGTGGTAGTTCATCAGGTGGXXXXXX
XXXXAATGCTGTGTAAAGGTTCGCCATGATAGATGTTCATTAAGGATGTCCACATGGCCN
ATGAATGTAAAGTTGATCACNATGGTAAAGAATGTTGCGTCAGATCCCATAGACCACCAC
CNCCAGAAGTTTGTTCATTAAGATGCCCACCAAAACATGAATGTAAATTTGATGATCATG
GTAAAAAATGTTGTGTAAAGATTCATTGTGATGAAGTTTGTGATTTAGATTGTGGTAGAG
GTTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGTGTCCGTTCAGAAAGACCAC
ACCCNCCNCAACATGAAAAATGTAATAAGAGATGCCCACCAGGCCATGAATGTAAAGTTG
ATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGATCTTTAA
GATGTCCNCCAAGACATGAATGTAGAGTCAACCNCTTTGGTGAAGAATGTTGTGTTAAAG
TTCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCATGAATGTAAAGTTGATCAAC
ATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGATCATTAAGATGTC
CNCCAAAACATGAATGTAGAATCAATCNCTTTGGTGAAGAATGCTGTGTTAAAAGTAGAA
ATGATTGTTTAACTTGTGAAGACTTAAACTGTGAAAGAAAAGGTTTACATTGTGCCATGA
AAACTGTACCNATTGATAAAG
sequence update 2002.10.25
Translated Amino Acid sequence 
fnfifnqfklkkkq*ltklkikk**RMLFLKNIGVFFMIFLVSKSYATDCNKITNEXXCH
KSSECIVINYTPCCGEQKWACSKGTFDTCTYENSCYXNSSNNQVVEVSNKCFNLDGFIKI
TTPTEYSCSDAXIKECALLGKSCSFQXNSCSNPTSCCPGESICEGLSSGSSTSGGGSSGG
TSGGSSSGGTSGGSSSG---

---CCVKVRHDRCSLRMSTWPMNVKLITMVKNVASDPIDHHXQKFVH*dahqnmnvnlmi
mvknvv*rfivmkfvi*ivvevlnvkldmmvqnvvsvqkdhtxxnmknvirdahqamnvk
linmeknvallpidhhqndl*dvxqdmnvestxlvknvvlkfttinvh*dvhqamnvkli
nmeknvallpidhhqndh*dvxqnmnvesixlvknavlkvemiv*lvkt*tvkekvyivp
*klyxlik
Translated Amino Acid sequence (All Frames) 
Frame A:
iqfyf*siqikkktitn*vknkkiikdvvskkywsifydipcikilcdgl**ny**xxms
*ifgmycyklytmlw*tkmgll*ryi*ylyi*k*llxkfiq*psc*sik*mlqs*wiy*n
ynsnrvflf*cqx*rmclir*im*fpkxllfksnfllpr*inl*rfklw*fniwwwfirw
yigw*firwyirw*firw---

---nav*rfamidvh*gcphgx*m*s*sxw*rmlrqip*tttxrslfikmptkt*m*i**
sw*kmlckdsl**sl*frlw*rf*m*n*t*wfkmlcpfrkttpxxt*km**emptrp*m*
s*stwkrmlrccp*tttkmifkmsxkt*m*sqplw*rmlc*sspr*mfiemstrp*m*s*
stwkrmlrccp*tttkmiikmsxkt*m*nqslw*rmlc*k*k*lfnl*rlkl*kkrftlc
henctx**

Frame B:
fnfifnqfklkkkq*ltklkikk**RMLFLKNIGVFFMIFLVSKSYATDCNKITNEXXCH
KSSECIVINYTPCCGEQKWACSKGTFDTCTYENSCYXNSSNNQVVEVSNKCFNLDGFIKI
TTPTEYSCSDAXIKECALLGKSCSFQXNSCSNPTSCCPGESICEGLSSGSSTSGGGSSGG
TSGGSSSGGTSGGSSSG---

---mlckgsp**mfikdvhmaxeckvdhxgkeccvrshrppppevcslrcppkheckfdd
hgkkccvkihcdevcdldcgrgfeckirhdgskccvrserphppqhekcnkrcppgheck
vdqhgkeccvvahrpppk*slrcpprhecrvnxfgeeccvkvhhdkcslrcppgheckvd
qhgkeccvvahrpppk*slrcppkhecrinxfgeeccvksrndcltcedlncerkglhca
mktvpidk

Frame C:
silflinsn*kknnn*ls*k*knnkgccf*kileyfl*yslyqnpmrrivikllmxxnvi
nlrnvll*iihhvvvnknglalkvhlilvhmkivvixihpitkllkyqinasilmdllkl
qlqpsilvlmpxlknvpy*vnhvvskxtlvqiqlpvaqvnqfvkv*alvvqhlvvvhqvv
hrvvvhqvvhqvvvhqv---

---CCVKVRHDRCSLRMSTWPMNVKLITMVKNVASDPIDHHXQKFVH*dahqnmnvnlmi
mvknvv*rfivmkfvi*ivvevlnvkldmmvqnvvsvqkdhtxxnmknvirdahqamnvk
linmeknvallpidhhqndl*dvxqdmnvestxlvknvvlkfttinvh*dvhqamnvkli
nmeknvallpidhhqndh*dvxqnmnvesixlvknavlkvemiv*lvkt*tvkekvyivp
*klyxlik
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHJ161 (CHJ161Q) /CSM/CH/CHJ1-C/CHJ161Q.Seq.d/                       2284   0.0
CHH493 (CHH493Q) /CSM/CH/CHH4-D/CHH493Q.Seq.d/                       1366   0.0
CHI435 (CHI435Q) /CSM/CH/CHI4-B/CHI435Q.Seq.d/                       1356   0.0
SFI482 (SFI482Q) /CSM/SF/SFI4-D/SFI482Q.Seq.d/                       1354   0.0
CHP472 (CHP472Q) /CSM/CH/CHP4-C/CHP472Q.Seq.d/                       1354   0.0
CHL565 (CHL565Q) /CSM/CH/CHL5-C/CHL565Q.Seq.d/                       1354   0.0
CHR436 (CHR436Q) /CSM/CH/CHR4-B/CHR436Q.Seq.d/                       1350   0.0
CHQ263 (CHQ263Q) /CSM/CH/CHQ2-C/CHQ263Q.Seq.d/                       1350   0.0
CHH323 (CHH323Q) /CSM/CH/CHH3-A/CHH323Q.Seq.d/                       1350   0.0
CHH267 (CHH267Q) /CSM/CH/CHH2-C/CHH267Q.Seq.d/                       1348   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U25144|U25144.1 Dictyostelium discoideum spore coat protein SP87 (PspD) gene, complete cds.  1273   0.0    10
AC117267|AC117267.2 Dictyostelium discoideum chromosome 2 map 5836255-5862024 strain AX4, complete sequence.  1273   0.0    11
M26239|M26239.1 D.discoideum spore coat protein SP60 gene, complete cds.    66   3e-14  4
X51892|X51892.1 Dictyostelium discoideum SP60 gene for spore coat protein.    66   9e-14  4
AC150239|AC150239.2 Sorex araneus clone SA_Ba-527L4, WORKING DRAFT SEQUENCE, 7 ordered pieces.    36   0.072  7
X52105|X52105.1 Dictyostelium discoideum SP60 gene for spore coat protein.    48   0.13   3
AC141955|AC141955.2 Rattus norvegicus clone CH230-418P10, WORKING DRAFT SEQUENCE, 29 unordered pieces.    48   0.13   4
AC146784|AC146784.21 Medicago truncatula clone mth2-172c4, WORKING DRAFT SEQUENCE, 2 ordered pieces.    34   0.16   7
AC127160|AC127160.3 Rattus norvegicus clone CH230-460D14, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    48   0.39   1
AC111000|AC111000.3 Homo sapiens BAC clone RP13-644M16 from 4, complete sequence.    32   0.45   9
dna update 2005. 1. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...   519   e-148
(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...   178   3e-43
X52105_1(X52105|pid:none) Dictyostelium discoideum SP60 gene for...   107   7e-22
(Q6TU45) RecName: Full=Probable spore coat protein sigD; AltName...    93   2e-17
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    81   9e-14
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;        68   8e-10
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    65   7e-09
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    65   7e-09
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...    64   1e-08
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...    59   3e-07
protein update 2009. 4. 3
PSORT 

 psg: 1.02  gvh: 0.68  alm: 0.51  top: 0.33  tms: 0.00  mit: 0.38  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: extracellular, including cell wall
	 12.0 %: vacuolar
	  8.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoplasmic
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for CHJ161 is exc
5' end seq. ID CHJ161F
5' end seq. 
>CHJ161F.Seq
ATTCAATTTTATTTTTAATCAATTCAAATTAAAAAAAAAACAATAACTAACTAAGTTAAA
AATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTATGATATT
CCTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAANAANAATGTCA
TAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGTGGTGAACAAAAATGGGC
TTGCTCTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATANAAATTCATC
CAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTATTAAAAT
TACAACTCCAACCGAGTATTCTTGTTCTGATGCCAANATTAAAGAATGTGCCTTATTAGG
TAAATCATGTAGTTTCCAAAANAACTCTTGTTCAAATCCAACTTCCTGTTGCCCAGGTGA
ATCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAACATCTGGTGGTGGTTCATCAGGTGG
TACATCGGGTGGTAGTTCATCAGGTGGTACATCAGGTGGTAGTTCATCAGGTGGNNNNNN
NNNN
Length of 5' end seq. 604
3' end seq. ID CHJ161Z
3' end seq. 
>CHJ161Z.Seq
NNNNNNNNNNAATGCTGTGTAAAGGTTCGCCATGATAGATGTTCATTAAGGATGTCCACA
TGGCCNATGAATGTAAAGTTGATCACNATGGTAAAGAATGTTGCGTCAGATCCCATAGAC
CACCACCNCCAGAAGTTTGTTCATTAAGATGCCCACCAAAACATGAATGTAAATTTGATG
ATCATGGTAAAAAATGTTGTGTAAAGATTCATTGTGATGAAGTTTGTGATTTAGATTGTG
GTAGAGGTTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGTGTCCGTTCAGAAA
GACCACACCCNCCNCAACATGAAAAATGTAATAAGAGATGCCCACCAGGCCATGAATGTA
AAGTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGAT
CTTTAAGATGTCCNCCAAGACATGAATGTAGAGTCAACCNCTTTGGTGAAGAATGTTGTG
TTAAAGTTCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCATGAATGTAAAGTTG
ATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGATCATTAA
GATGTCCNCCAAAACATGAATGTAGAATCAATCNCTTTGGTGAAGAATGCTGTGTTAAAA
GTAGAAATGATTGTTTAACTTGTGAAGACTTAAACTGTGAAAGAAAAGGTTTACATTGTG
CCATGAAAACTGTACCNATTGATAAAG
Length of 3' end seq. 747
Connected seq. ID CHJ161P
Connected seq. 
>CHJ161P.Seq
ATTCAATTTTATTTTTAATCAATTCAAATTAAAAAAAAAACAATAACTAACTAAGTTAAA
AATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTATGATATT
CCTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAANAANAATGTCA
TAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGTGGTGAACAAAAATGGGC
TTGCTCTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATANAAATTCATC
CAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTATTAAAAT
TACAACTCCAACCGAGTATTCTTGTTCTGATGCCAANATTAAAGAATGTGCCTTATTAGG
TAAATCATGTAGTTTCCAAAANAACTCTTGTTCAAATCCAACTTCCTGTTGCCCAGGTGA
ATCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAACATCTGGTGGTGGTTCATCAGGTGG
TACATCGGGTGGTAGTTCATCAGGTGGTACATCAGGTGGTAGTTCATCAGGTGG------
----AATGCTGTGTAAAGGTTCGCCATGATAGATGTTCATTAAGGATGTCCACATGGCCN
ATGAATGTAAAGTTGATCACNATGGTAAAGAATGTTGCGTCAGATCCCATAGACCACCAC
CNCCAGAAGTTTGTTCATTAAGATGCCCACCAAAACATGAATGTAAATTTGATGATCATG
GTAAAAAATGTTGTGTAAAGATTCATTGTGATGAAGTTTGTGATTTAGATTGTGGTAGAG
GTTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGTGTCCGTTCAGAAAGACCAC
ACCCNCCNCAACATGAAAAATGTAATAAGAGATGCCCACCAGGCCATGAATGTAAAGTTG
ATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGATCTTTAA
GATGTCCNCCAAGACATGAATGTAGAGTCAACCNCTTTGGTGAAGAATGTTGTGTTAAAG
TTCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCATGAATGTAAAGTTGATCAAC
ATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGATCATTAAGATGTC
CNCCAAAACATGAATGTAGAATCAATCNCTTTGGTGAAGAATGCTGTGTTAAAAGTAGAA
ATGATTGTTTAACTTGTGAAGACTTAAACTGTGAAAGAAAAGGTTTACATTGTGCCATGA
AAACTGTACCNATTGATAAAG
Length of connected seq. 1331
Full length Seq ID -
Full length Seq. -
Length of full length seq. -