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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH182
Library AF
(Link to library)
Clone ID AFH182
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16381-1
Original site URL
Representative seq. ID AFH182P
(Link to Original site)
Representative DNA sequence 
>AFH182 (AFH182Q) /CSM/AF/AFH1-D/AFH182Q.Seq.d/
ATTTTGTTCNTATTCAAATTAAATNTCAATNCAATTAAAAAANGTTAAAATTAAATTTGT
AAAATCAATTTGTANCAAAANCTAGTATTTTAAAAAAAAAAAAACCAAAAAAAAAAXXXX
XXXXXXTAAGAAAGCAAGGTGTGATNATGTCGAATGTCCNNATTTCCATANATGTGTTTA
AANACGTGGTGGNATCTTAAGNTGTGAATTTNATCCCCCAAGACAACCNAGATCCCTTGA
TTGGGCTGAAAATGAAAATGATGATCGNGACTATGATGATCGNGACTATGATGATGATGA
ANATGATGGTGATTATGATGGCCGTGACGGTGACTATGATGGNGATTATGATGGNGATTA
TGATGAGGCCCATTATTATGG
sequence update 2001. 6. 2
Translated Amino Acid sequence 
fvxiqikxqxn*kxlklnl*NQFVXKXSILKKKKPKKK---

---RKQGVXMSNVXISIXVFKXVVXS*xvnxipqdnxdpliglkmkmmixtmmixtmmmm
xmmvimmavtvtmmximmximmrpiim
Translated Amino Acid sequence (All Frames) 
Frame A:
ilfxfklnxnxikkx*n*ickinlxqxlvf*kkknqkk---

---*eskv*xcrmsxfpxmclxtwwxlkx*ixspkttxip*lg*k*k**sxl**sxl***
*x*w*l*wp*r*l*wxl*wxl**gpllw

Frame B:
fcsysn*xsxqlkxvkikfvksicxkx*yfkkkktkkk---

---kkarcdxvecpxfhxcv*xrggilxcefxpprqprsldwaenenddrdyddrdyddd
exdgdydgrdgdydgdydgdydeahyy

Frame C:
fvxiqikxqxn*kxlklnl*NQFVXKXSILKKKKPKKK---

---RKQGVXMSNVXISIXVFKXVVXS*xvnxipqdnxdpliglkmkmmixtmmixtmmmm
xmmvimmavtvtmmximmximmrpiim
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH182 (AFH182Q) /CSM/AF/AFH1-D/AFH182Q.Seq.d/                        182   3e-45
AFF284 (AFF284Q) /CSM/AF/AFF2-D/AFF284Q.Seq.d/                        163   2e-39
CHA309 (CHA309Q) /CSM/CH/CHA3-A/CHA309Q.Seq.d/                        161   9e-39
SLJ720 (SLJ720Q) /CSM/SL/SLJ7-A/SLJ720Q.Seq.d/                        159   4e-38
SLI767 (SLI767Q) /CSM/SL/SLI7-C/SLI767Q.Seq.d/                        159   4e-38
SLI753 (SLI753Q) /CSM/SL/SLI7-C/SLI753Q.Seq.d/                        159   4e-38
SLI573 (SLI573Q) /CSM/SL/SLI5-D/SLI573Q.Seq.d/                        159   4e-38
SLI384 (SLI384Q) /CSM/SL/SLI3-D/SLI384Q.Seq.d/                        159   4e-38
SLH820 (SLH820Q) /CSM/SL/SLH8-A/SLH820Q.Seq.d/                        159   4e-38
SLH722 (SLH722Q) /CSM/SL/SLH7-A/SLH722Q.Seq.d/                        159   4e-38
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26239|M26239.1 D.discoideum spore coat protein SP60 gene, complete cds.   159   8e-42  2
X51892|X51892.1 Dictyostelium discoideum SP60 gene for spore coat protein.   159   1e-41  2
AC114061|AC114061.4 Rattus norvegicus clone CH230-197F21, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    42   5.6    1
dna update 2003. 8.31
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...    85   9e-16
protein update 2008.11.21
PSORT 

 psg: 0.08  gvh: 0.25  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.48  mip: 0.00
 nuc: 0.43  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 76.0 %: nuclear
	 12.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for AFH182 is nuc
5' end seq. ID AFH182F
5' end seq. 
>AFH182F.Seq
ATTTTGTTCNTATTCAAATTAAATNTCAATNCAATTAAAAAANGTTAAAATTAAATTTGT
AAAATCAATTTGTANCAAAANCTAGTATTTTAAAAAAAAAAAAACCAAAAAAAAAA----
------
Length of 5' end seq. 116
3' end seq. ID AFH182Z
3' end seq. 
>AFH182Z.Seq
----------TAAGAAAGCAAGGTGTGATNATGTCGAATGTCCNNATTTCCATANATGTG
TTTAAANACGTGGTGGNATCTTAAGNTGTGAATTTNATCCCCCAAGACAACCNAGATCCC
TTGATTGGGCTGAAAATGAAAATGATGATCGNGACTATGATGATCGNGACTATGATGATG
ATGAANATGATGGTGATTATGATGGCCGTGACGGTGACTATGATGGNGATTATGATGGNG
ATTATGATGAGGCCCATTATTATGG
Length of 3' end seq. 255
Connected seq. ID AFH182P
Connected seq. 
>AFH182P.Seq
ATTTTGTTCNTATTCAAATTAAATNTCAATNCAATTAAAAAANGTTAAAATTAAATTTGT
AAAATCAATTTGTANCAAAANCTAGTATTTTAAAAAAAAAAAAACCAAAAAAAAAA----
------TAAGAAAGCAAGGTGTGATNATGTCGAATGTCCNNATTTCCATANATGTGTTTA
AANACGTGGTGGNATCTTAAGNTGTGAATTTNATCCCCCAAGACAACCNAGATCCCTTGA
TTGGGCTGAAAATGAAAATGATGATCGNGACTATGATGATCGNGACTATGATGATGATGA
ANATGATGGTGATTATGATGGCCGTGACGGTGACTATGATGGNGATTATGATGGNGATTA
TGATGAGGCCCATTATTATGG
Length of connected seq. 371
Full length Seq ID -
Full length Seq. -
Length of full length seq. -