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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH242
Library AF
(Link to library)
Clone ID AFH242
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U09841-1
Original site URL
Representative seq. ID AFH242P
(Link to Original site)
Representative DNA sequence 
>AFH242 (AFH242Q) /CSM/AF/AFH2-B/AFH242Q.Seq.d/
ACTGTTTGGCCTACTGGGTTTACAACACTGTTTATTCATATACTTCACTTTGAAGAATAA
TAAAAAAAAATAAAAAAAAAGAAAAATGAGTAATGTTTATGATATTGTTGTAATTGGATC
AGGTGTTTCAGGTTTAATGTGTGGTTATAAATTAGAAAAGGATGGATATAAAGTTTTAGT
TGTTGAAGCAAATAATATTATTGGTGGTAGAACTAGACAAGATTTTAATTTTACAAGTTA
TCCAGTTGAATTGGGTGGTGAAATGATTCATGGTGGTGATACATTATATTATCGTTTAGC
AATGGAGAATAAATGGGAACTATTTGAAGTATTCTCAATGGATTTATTTAATCTCACCAA
TTAATTCCACTTATTTATATTTAGGACGTGAAAGAAAXXXXXXXXXXTTCAAGCCCAACG
TGTGGTCGTAACTGTGCCACTACAAATCTTAAAAGATGGTGATATCACCTTCACCCCAGA
ATTACCAGAGAGAAAGAAAATCGCAATTAAAACCATCGGTATGGACGGTGGTATGAAAAT
CATCGCTAAATTCAATAAAAAGTTTTGGTTAAATAATTGTCAATTGGTTCTTTGTGGTGA
TAGTCCAGTACCTCAAATTTGGATGGACGGTCCACCATATCGTCCACTCGTTCCTGGACA
ACCTTCTGAATATGTTTCAGTTGGCTTCATCACTGGTGACCAAGCTAAAGCCATCAGTGC
TCTATCACCACAAAAACAAATTCGTACTTTCCTCGATCAATTAGATGCTATGTTTGGTAC
TTCTGAAAATGGTTGGACACCTGCCTCTGACTCTTTTATCTCACATATGGTTTATGATTG
GCAAAAGAATCCATTCGTACGTGGTGCCTATTCTTATCCATCAATTATTCCTTCAACTTA
TCCATATAAAAATTATCCAAATGAAATTTTAGCAGAGCCTATTGATAATAAATTATTCTT
TGCAGGTGAAGCAACAGCTACAACATACGATCTCTCAACTATAAATGGAGCTCTTGAAAC
TGGTGTTAGAGTCTAGAAGAATTAAAAACTTCTTTACCAATTTCAAACAAGTTACAATCA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
claywvyntvysytsl*riikknkkkek*vmfmill*ldqvfqv*cvvin*krmdikf*l
lkqiillvveldkililqviqlnwvvk*fmvvihyiiv*QWRINGNYLKYSQWIYLISPI
NSTYLYLGRER---

---QAQRVVVTVPLQILKDGDITFTPELPERKKIAIKTIGMDGGMKIIAKFNKKFWLNNC
QLVLCGDSPVPQIWMDGPPYRPLVPGQPSEYVSVGFITGDQAKAISALSPQKQIRTFLDQ
LDAMFGTSENGWTPASDSFISHMVYDWQKNPFVRGAYSYPSIIPSTYPYKNYPNEILAEP
IDNKLFFAGEATATTYDLSTINGALETGVRV*kn*kllyqfqtsyn
Translated Amino Acid sequence (All Frames) 
Frame A:
tvwptgfttlfihilhfee**kkikkkkne*cl*yccnwircfrfnvwl*irkgwi*sfs
c*sk*yyww*n*trf*fyklss*igw*ndsww*yiilsfsnge*mgti*silngfi*shq
lipliyi*dvke---

---fkpnvws*lchyks*kmvispspqnyqrerksqlkpsvwtvv*ksslnsiksfg*ii
vnwffvvivqylkfgwtvhhivhsfldnllnmfqlasslvtklkpsvlyhhknkfvlssi
n*mlclvllkmvghlpltllshiwfmigkrihsyvvpilihqlflqlihikiiqmkf*qs
lliinyslqvkqqlqhtisql*mellklvlesrriknfftnfkqvti

Frame B:
lfgllglqhclfiyftlknnkkk*kkrkmsnvydivvigsgvsglmcgyklekdgykvlv
veanniiggrtrqdfnftsypvelggemihggdtlyyrlamenkwelfevfsmdlfnltn
*fhlfifrt*kk---

---ssptcgrncattnlkrw*yhlhpritrekenrn*nhrygrwyenhr*iq*kvlvk*l
sigslw**sstsnldgrstisstrswttf*icfswlhhw*ps*shqcsittktnsyfprs
ircyvwyf*kwldtcl*lfyltygl*lakesirtwclflsinysfnlsi*klsk*nfsra
y***iilcr*snsynirslnykwss*nwc*sleelktslpisnklqs

Frame C:
claywvyntvysytsl*riikknkkkek*vmfmill*ldqvfqv*cvvin*krmdikf*l
lkqiillvveldkililqviqlnwvvk*fmvvihyiiv*QWRINGNYLKYSQWIYLISPI
NSTYLYLGRER---

---QAQRVVVTVPLQILKDGDITFTPELPERKKIAIKTIGMDGGMKIIAKFNKKFWLNNC
QLVLCGDSPVPQIWMDGPPYRPLVPGQPSEYVSVGFITGDQAKAISALSPQKQIRTFLDQ
LDAMFGTSENGWTPASDSFISHMVYDWQKNPFVRGAYSYPSIIPSTYPYKNYPNEILAEP
IDNKLFFAGEATATTYDLSTINGALETGVRV*kn*kllyqfqtsyn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH242 (AFH242Q) /CSM/AF/AFH2-B/AFH242Q.Seq.d/                       1911   0.0
VFD126 (VFD126Q) /CSM/VF/VFD1-B/VFD126Q.Seq.d/                       1334   0.0
VFD117 (VFD117Q) /CSM/VF/VFD1-A/VFD117Q.Seq.d/                       1334   0.0
AFN248 (AFN248Q) /CSM/AF/AFN2-B/AFN248Q.Seq.d/                       1332   0.0
VFE118 (VFE118Q) /CSM/VF/VFE1-A/VFE118Q.Seq.d/                       1296   0.0
VFI127 (VFI127Q) /CSM/VF/VFI1-B/VFI127Q.Seq.d/                       1287   0.0
VFL129 (VFL129Q) /CSM/VF/VFL1-B/VFL129Q.Seq.d/                       1285   0.0
AFC486 (AFC486Q) /CSM/AF/AFC4-D/AFC486Q.Seq.d/                       1281   0.0
VFF250 (VFF250Q) /CSM/VF/VFF2-C/VFF250Q.Seq.d/                       1279   0.0
AFE259 (AFE259Q) /CSM/AF/AFE2-C/AFE259Q.Seq.d/                       1267   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AP006628|AP006628.1 Onion yellows phytoplasma OY-M DNA, complete genome.    38   2e-05  23
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    34   2e-05  18
AB063521|AB063521.2 Wigglesworthia glossinidia endosymbiont of Glossina brevipalpis DNA, complete genome, section 1/2.    34   0.031  15
AC117081|AC117081.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    32   0.045  12
AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    30   0.12   14
AC123755|AC123755.3 Mus musculus clone RP24-228O15, WORKING DRAFT SEQUENCE, 5 unordered pieces.    34   0.14   9
AC090085|AC090085.4 Homo sapiens chromosome 18 clone CTD-2657N20 map 18, WORKING DRAFT SEQUENCE, 3 unordered pieces.    36   0.16   8
AC021506|AC021506.5 Homo sapiens chromosome 18, clone RP11-173L6, complete sequence.    36   0.18   7
AL731574|AL731574.10 Human DNA sequence from clone RP11-56I23 on chromosome 10.    48   0.27   1
AE016964|AE016964.1 Coxiella burnetii strain RSA 493, section 5 of 7 of the complete genome.    48   0.27   1
dna update 2004. 4. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP001337_2862(CP001337|pid:none) Chloroflexus aggregans DSM 9485...   100   1e-19
AL031774_2(AL031774|pid:none) Human DNA sequence from clone RP1-...    93   2e-17
AK028553_1(AK028553|pid:none) Mus musculus 0 day neonate skin cD...    92   3e-17
CR954203_358(CR954203|pid:none) Ostreococcus tauri strain OTTH05...    80   1e-13
(Q8C0L6) RecName: Full=Peroxisomal N(1)-acetyl-spermine/spermidi...    79   2e-13
AF226657_1(AF226657|pid:none) Homo sapiens peroxisomal N1-acetyl...    79   3e-13
AL834535_1(AL834535|pid:none) Homo sapiens mRNA; cDNA DKFZp434J2...    79   3e-13
AF312698_1(AF312698|pid:none) Homo sapiens peroxisomal N1-acetyl...    79   3e-13
AM049402_1(AM049402|pid:none) Mus musculus mRNA for peroxisomal ...    78   4e-13
CP000511_4313(CP000511|pid:none) Mycobacterium vanbaalenii PYR-1...    77   8e-13
protein update 2009. 4.21
PSORT 

 psg: 0.90  gvh: 0.33  alm: 0.43  top: 0.47  tms: 0.00  mit: 0.35  mip: 0.07
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 24.0 %: nuclear
	 16.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for AFH242 is cyt
5' end seq. ID AFH242F
5' end seq. 
>AFH242F.Seq
ACTGTTTGGCCTACTGGGTTTACAACACTGTTTATTCATATACTTCACTTTGAAGAATAA
TAAAAAAAAATAAAAAAAAAGAAAAATGAGTAATGTTTATGATATTGTTGTAATTGGATC
AGGTGTTTCAGGTTTAATGTGTGGTTATAAATTAGAAAAGGATGGATATAAAGTTTTAGT
TGTTGAAGCAAATAATATTATTGGTGGTAGAACTAGACAAGATTTTAATTTTACAAGTTA
TCCAGTTGAATTGGGTGGTGAAATGATTCATGGTGGTGATACATTATATTATCGTTTAGC
AATGGAGAATAAATGGGAACTATTTGAAGTATTCTCAATGGATTTATTTAATCTCACCAA
TTAATTCCACTTATTTATATTTAGGACGTGAAAGAAA----------
Length of 5' end seq. 397
3' end seq. ID AFH242Z
3' end seq. 
>AFH242Z.Seq
----------TTCAAGCCCAACGTGTGGTCGTAACTGTGCCACTACAAATCTTAAAAGAT
GGTGATATCACCTTCACCCCAGAATTACCAGAGAGAAAGAAAATCGCAATTAAAACCATC
GGTATGGACGGTGGTATGAAAATCATCGCTAAATTCAATAAAAAGTTTTGGTTAAATAAT
TGTCAATTGGTTCTTTGTGGTGATAGTCCAGTACCTCAAATTTGGATGGACGGTCCACCA
TATCGTCCACTCGTTCCTGGACAACCTTCTGAATATGTTTCAGTTGGCTTCATCACTGGT
GACCAAGCTAAAGCCATCAGTGCTCTATCACCACAAAAACAAATTCGTACTTTCCTCGAT
CAATTAGATGCTATGTTTGGTACTTCTGAAAATGGTTGGACACCTGCCTCTGACTCTTTT
ATCTCACATATGGTTTATGATTGGCAAAAGAATCCATTCGTACGTGGTGCCTATTCTTAT
CCATCAATTATTCCTTCAACTTATCCATATAAAAATTATCCAAATGAAATTTTAGCAGAG
CCTATTGATAATAAATTATTCTTTGCAGGTGAAGCAACAGCTACAACATACGATCTCTCA
ACTATAAATGGAGCTCTTGAAACTGGTGTTAGAGTCTAGAAGAATTAAAAACTTCTTTAC
CAATTTCAAACAAGTTACAATCA
Length of 3' end seq. 673
Connected seq. ID AFH242P
Connected seq. 
>AFH242P.Seq
ACTGTTTGGCCTACTGGGTTTACAACACTGTTTATTCATATACTTCACTTTGAAGAATAA
TAAAAAAAAATAAAAAAAAAGAAAAATGAGTAATGTTTATGATATTGTTGTAATTGGATC
AGGTGTTTCAGGTTTAATGTGTGGTTATAAATTAGAAAAGGATGGATATAAAGTTTTAGT
TGTTGAAGCAAATAATATTATTGGTGGTAGAACTAGACAAGATTTTAATTTTACAAGTTA
TCCAGTTGAATTGGGTGGTGAAATGATTCATGGTGGTGATACATTATATTATCGTTTAGC
AATGGAGAATAAATGGGAACTATTTGAAGTATTCTCAATGGATTTATTTAATCTCACCAA
TTAATTCCACTTATTTATATTTAGGACGTGAAAGAAA----------TTCAAGCCCAACG
TGTGGTCGTAACTGTGCCACTACAAATCTTAAAAGATGGTGATATCACCTTCACCCCAGA
ATTACCAGAGAGAAAGAAAATCGCAATTAAAACCATCGGTATGGACGGTGGTATGAAAAT
CATCGCTAAATTCAATAAAAAGTTTTGGTTAAATAATTGTCAATTGGTTCTTTGTGGTGA
TAGTCCAGTACCTCAAATTTGGATGGACGGTCCACCATATCGTCCACTCGTTCCTGGACA
ACCTTCTGAATATGTTTCAGTTGGCTTCATCACTGGTGACCAAGCTAAAGCCATCAGTGC
TCTATCACCACAAAAACAAATTCGTACTTTCCTCGATCAATTAGATGCTATGTTTGGTAC
TTCTGAAAATGGTTGGACACCTGCCTCTGACTCTTTTATCTCACATATGGTTTATGATTG
GCAAAAGAATCCATTCGTACGTGGTGCCTATTCTTATCCATCAATTATTCCTTCAACTTA
TCCATATAAAAATTATCCAAATGAAATTTTAGCAGAGCCTATTGATAATAAATTATTCTT
TGCAGGTGAAGCAACAGCTACAACATACGATCTCTCAACTATAAATGGAGCTCTTGAAAC
TGGTGTTAGAGTCTAGAAGAATTAAAAACTTCTTTACCAATTTCAAACAAGTTACAATCA
Length of connected seq. 1070
Full length Seq ID -
Full length Seq. -
Length of full length seq. -