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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHS418
Library CH
(Link to library)
Clone ID CHS418
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID CHS418P
(Link to Original site)
Representative DNA sequence 
>CHS418 (CHS418Q) /CSM/CH/CHS4-A/CHS418Q.Seq.d/
AATTCAGTAGTTCAATTTAATTTATAATAATAATAATAGTATAAATATATAAAGATTAAT
TTTTTTGTATATAAATTTTTGTAAATAAATAAATAAATAATAATTTTTTTTTTAATTCAC
ACACATACATATAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTATTGAT
TTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTATTGCA
AATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATACAGAACGTTTA
ATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTGATGCT
AAACGTTTAATTGGTCGTNAAXXXXXXXXXXCCAGGTGTATTAATTCAAGTTTATGAAGG
TGAACGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCC
ACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTAT
CTTAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAA
TGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATT
CAAACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTA
TGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTC
AGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCA
AACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCC
AATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGG
TATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAA
TTTTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAA
TCAATAAAGCTTCTCATATAT
sequence update 2002.10.25
Translated Amino Acid sequence 
nsvvqfnl*****ykyikinffvykfl*ink*iiifflihthtyky*iiky*KMSSIGID
LGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDA
KRLIGRX---

---PGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNVSAED
KSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFTVKNS
IKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMSKLYQ
EGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*kiis**nq*sfsy
Translated Amino Acid sequence (All Frames) 
Frame A:
nsvvqfnl*****ykyikinffvykfl*ink*iiifflihthtyky*iiky*KMSSIGID
LGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDA
KRLIGRX---

---PGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNVSAED
KSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFTVKNS
IKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMSKLYQ
EGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*kiis**nq*sfsy

Frame B:
iq*fnliynnnnsini*rliflyinfck*ink**fff*fthihinik*lnikkchqlvli
*vqhihvlvfgkmivlkllqmikvieplhlmlhlliqnv*lvmlqriklq*iqpiqylml
nv*lvv---

---qvy*fkfmkvnvl*pkiiiywvnlnflvfhqhqevyhklklhlmlmqmvs*myqlki
nqlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkmnnkkivlnqrinwkimhsllkiq
lkmkklqpkfqiqinqllnlklkvysng*nqikpqkrmnmkir*kp*kqlsiqs*vnsik
kevchkvvvcqvvcqmihqrvqttk*tn*idgifqrktslsnrflkk**vskinkashi

Frame C:
fsssi*fiiiiiv*iykd*ffci*ifvnk*innnfffnshtyi*ilnn*ilknvinwy*f
rynifmcwclak*sc*nyck*sr**nhsilcciy*yrtfnw*cckesscnesnqysi*c*
tfnwsx---

---rcinssl*r*tcydqr**fig*i*tfwyststkrctts*syi*c*ckwylkcis*r*
inw*qtkdynyq**rssfkrrn*kngc*creiqttr*ttkrsc*ike*igklcihc*kfn
*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i*r*deslrsscqsnhe*tlsr
rryatrwwyarwyvk*ftkefkqqsrrirlteffrgkhlyqidflknnklvksiklliy
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ260 (VHQ260Q) /CSM/VH/VHQ2-C/VHQ260Q.Seq.d/                       1407   0.0
VHP719 (VHP719Q) /CSM/VH/VHP7-A/VHP719Q.Seq.d/                       1407   0.0
VHP716 (VHP716Q) /CSM/VH/VHP7-A/VHP716Q.Seq.d/                       1407   0.0
VHP437 (VHP437Q) /CSM/VH/VHP4-B/VHP437Q.Seq.d/                       1407   0.0
VHP266 (VHP266Q) /CSM/VH/VHP2-C/VHP266Q.Seq.d/                       1407   0.0
VHP236 (VHP236Q) /CSM/VH/VHP2-B/VHP236Q.Seq.d/                       1407   0.0
VHO677 (VHO677Q) /CSM/VH/VHO6-D/VHO677Q.Seq.d/                       1407   0.0
VHO566 (VHO566Q) /CSM/VH/VHO5-C/VHO566Q.Seq.d/                       1407   0.0
VHN681 (VHN681Q) /CSM/VH/VHN6-D/VHN681Q.Seq.d/                       1407   0.0
VHN672 (VHN672Q) /CSM/VH/VHN6-C/VHN672Q.Seq.d/                       1407   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1407   0.0    1
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.  1400   0.0    1
AF221541|AF221541.1 Cryptosporidium serpentis heat shock protein 70 (HSP70) gene, partial cds.   145   2e-88  8
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.   242   1e-86  5
AY954892|AY954892.1 Cryptosporidium andersoni strain HenanZhzhca heat shock protein 70 gene, partial cds.   123   4e-79  8
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   242   1e-78  9
AY954893|AY954893.1 Cryptosporidium andersoni strain changchunca heat shock protein 70 gene, partial cds.   129   5e-78  8
DQ060426|DQ060426.1 Cryptosporidium andersoni strain zzca heat shock protein 70-like gene, partial sequence.   127   1e-77  7
AY954894|AY954894.1 Cryptosporidium andersoni strain bjcm heat shock protein 70 gene, partial cds.   117   2e-77  8
AX536934|AX536934.1 Sequence 535 from Patent WO02064766.          123   5e-75  5
dna update 2005.11.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   345   1e-93
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   345   1e-93
AF033852_1(AF033852|pid:none) Spinacia oleracea cytosolic heat s...   251   4e-65
FB790108_1(FB790108|pid:none) Sequence 9381 from Patent WO200803...   250   5e-65
EF506932_1(EF506932|pid:none) Fasciola hepatica heat shock prote...   249   9e-65
FB790112_1(FB790112|pid:none) Sequence 9385 from Patent WO200803...   249   1e-64
(P22953) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   249   1e-64
S46302(S46302;S41402;JA0169;S36623) dnaK-type molecular chaperon...   249   1e-64
AY035123_1(AY035123|pid:none) Arabidopsis thaliana putative dnaK...   249   1e-64
EF506931_1(EF506931|pid:none) Fasciola gigantica heat shock prot...   248   2e-64
protein update 2009. 4.13
PSORT 

 psg: 0.87  gvh: 0.35  alm: 0.46  top: 0.27  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for CHS418 is cyt
5' end seq. ID CHS418F
5' end seq. 
>CHS418F.Seq
AATTCAGTAGTTCAATTTAATTTATAATAATAATAATAGTATAAATATATAAAGATTAAT
TTTTTTGTATATAAATTTTTGTAAATAAATAAATAAATAATAATTTTTTTTTTAATTCAC
ACACATACATATAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTATTGAT
TTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTATTGCA
AATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATACAGAACGTTTA
ATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTGATGCT
AAACGTTTAATTGGTCGTNAANNNNNNNNNN
Length of 5' end seq. 391
3' end seq. ID CHS418Z
3' end seq. 
>CHS418Z.Seq
NNNNNNNNNNCCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGA
TAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTACC
ACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGA
TAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAA
AGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACA
AAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTC
AATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATC
TGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATA
TGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCA
AGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAA
GAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCTC
TATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTCTCATATAT
Length of 3' end seq. 720
Connected seq. ID CHS418P
Connected seq. 
>CHS418P.Seq
AATTCAGTAGTTCAATTTAATTTATAATAATAATAATAGTATAAATATATAAAGATTAAT
TTTTTTGTATATAAATTTTTGTAAATAAATAAATAAATAATAATTTTTTTTTTAATTCAC
ACACATACATATAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTATTGAT
TTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTATTGCA
AATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATACAGAACGTTTA
ATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTGATGCT
AAACGTTTAATTGGTCGTNAA----------CCAGGTGTATTAATTCAAGTTTATGAAGG
TGAACGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCC
ACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTAT
CTTAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAA
TGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATT
CAAACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTA
TGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTC
AGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCA
AACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCC
AATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGG
TATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAA
TTTTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAA
TCAATAAAGCTTCTCATATAT
Length of connected seq. 1091
Full length Seq ID -
Full length Seq. -
Length of full length seq. -