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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHS421
Library CH
(Link to library)
Clone ID CHS421
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16327-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>CHS421 (CHS421Q) /CSM/CH/CHS4-A/CHS421Q.Seq.d/
XXXXXXXXXXAAAAAAAAAAAACAACCAAAATATTATATTATTTTTTGTAAATATAAATT
AAAAAAATGAAATTCCAACATACATTTATTGCATTATTATCACTATTAACATATGCAAAT
GCATATGATTATTTCNCAACAACATTGGCCAATCAAAATCCAGTTTGTGCTTCAGTAGAT
GTCATACAAAATGTTTGTAATGAGGTTTGTGGTAGATTTGTCCGTTACATTCNTGATGCT
ACCAATACAAATCAATTCACTTTTGCCGAATATACTACAAACCAATGTACTGTTCAAGTA
ACACCAGNTGTAACAAATACATTTACTTGCGNTGATCAAACTTCTTCACATGCANTCGGT
TCTGATTGGAGTGGAGTTTGTAAAATCANTGCCACACCCGCACCAACAGTTACACCAACA
GTTACACCAACAGTTACACCAACAGTAACACCAACACCAACAAATACACCAAATCCAACC
CCATCCCAAACATCAACAACAACTGGTTCTGCTTCAACTGTTGTTGCAAGTTTATCANTC
ATTATTT
sequence update 2002. 9.10
Translated Amino Acid sequence 
---kkkttkilyyfl*i*IKKMKFQHTFIALLSLLTYANAYDYFXTTLANQNPVCASVDV
IQNVCNEVCGRFVRYIXDATNTNQFTFAEYTTNQCTVQVTPXVTNTFTCXDQTSSHAXGS
DWSGVCKIXATPAPTVTPTVTPTVTPTVTPTPTNTPNPTPSQTSTTTGSASTVVASLSXI
I
Translated Amino Acid sequence (All Frames) 
Frame A:
---kkknnqniilffvnin*kneiptyiyciiitinickci*lfxnnigqsksslcfsrc
htkcl**glw*icplhs*cyqyksihfcriyykpmycssntxcnkyiylr*snfftcxrf
*lewsl*nxchtrtnsytnsytnsytnsntntnkytksnpipninnnwfcfnccckfixh
y


Frame B:
---kkkttkilyyfl*i*IKKMKFQHTFIALLSLLTYANAYDYFXTTLANQNPVCASVDV
IQNVCNEVCGRFVRYIXDATNTNQFTFAEYTTNQCTVQVTPXVTNTFTCXDQTSSHAXGS
DWSGVCKIXATPAPTVTPTVTPTVTPTVTPTPTNTPNPTPSQTSTTTGSASTVVASLSXI
I


Frame C:
---kkkqpkyyiifckyklkk*nsnihllhyyhy*hmqmhmiisqqhwpikiqfvlq*ms
ykmfvmrfvvdlsvtfxmlpiqinsllpnilqtnvlfk*hqx*qihllaxikllhmxsvl
igvefvksxphphqqlhqqlhqqlhqq*hqhqqihqiqphpkhqqqlvllqlllqvyxsl
f
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS421 (CHS421Q) /CSM/CH/CHS4-A/CHS421Q.Seq.d/                        957   0.0
SHK114 (SHK114Q) /CSM/SH/SHK1-A/SHK114Q.Seq.d/                        952   0.0
SSI759 (SSI759Q) /CSM/SS/SSI7-C/SSI759Q.Seq.d/                        950   0.0
SSI680 (SSI680Q) /CSM/SS/SSI6-D/SSI680Q.Seq.d/                        950   0.0
SSI169 (SSI169Q) /CSM/SS/SSI1-C/SSI169Q.Seq.d/                        950   0.0
SSC612 (SSC612Q) /CSM/SS/SSC6-A/SSC612Q.Seq.d/                        950   0.0
SSC554 (SSC554Q) /CSM/SS/SSC5-C/SSC554Q.Seq.d/                        950   0.0
SLG216 (SLG216Q) /CSM/SL/SLG2-A/SLG216Q.Seq.d/                        950   0.0
SHG613 (SHG613Q) /CSM/SH/SHG6-A/SHG613Q.Seq.d/                        950   0.0
SHF886 (SHF886Q) /CSM/SH/SHF8-D/SHF886Q.Seq.d/                        950   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X15980|X15980.1 Dictyostelium discoideum D19 gene 5'-flanking region.   894   0.0    2
M20909|M20909.1 Dictyostelium discoideum cell surface glycoprotein PsA precursor (D19) gene, complete cds.   894   0.0    2
A98322|A98322.1 Sequence 1 from Patent WO9913081.                 111   2e-20  1
BD075065|BD075065.1 Expression of gonadotropin in Dictyostelium.   111   2e-20  1
A21625|A21625.1 expression vector pMUW1630.                       105   1e-18  1
U20661|U20661.1 Dictyostelium discoideum unknown internal repeat protein gene, complete cds, and unknown orf1, orf2 and orf3 genes, partial cds.    66   8e-07  1
AF224509|AF224509.3 Bacteroides cellulosolvens cellulosomal scaffoldin precursor (cipBc), cellulosomal anchoring scaffoldin B (scaB), and sodium/calcium exchanger protein genes, complete cds.    44   5e-04  4
U16308|U16308.1 Caldocellum saccharolyticum endoglucanase/mannanase (celC) pseudogene, complete cds.    54   8e-04  2
AC116215|AC116215.5 Rattus norvegicus clone CH230-98H24, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    54   0.003  1
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    38   0.030  2
dna update 2005.11.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54LC3) RecName: Full=Ponticulin-like protein C2; Flags: Precur...    49   1e-04
A98322_1(A98322|pid:none) Sequence 1 from Patent WO9913081.            41   0.023
protein update 2009. 1. 4
PSORT 

 psg: 0.96  gvh: 0.78  alm: 0.44  top: 0.17  tms: 0.00  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: endoplasmic reticulum
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for CHS421 is exc
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID CHS421Z
3' end seq. 
>CHS421Z.Seq
NNNNNNNNNNAAAAAAAAAAAACAACCAAAATATTATATTATTTTTTGTAAATATAAATT
AAAAAAATGAAATTCCAACATACATTTATTGCATTATTATCACTATTAACATATGCAAAT
GCATATGATTATTTCNCAACAACATTGGCCAATCAAAATCCAGTTTGTGCTTCAGTAGAT
GTCATACAAAATGTTTGTAATGAGGTTTGTGGTAGATTTGTCCGTTACATTCNTGATGCT
ACCAATACAAATCAATTCACTTTTGCCGAATATACTACAAACCAATGTACTGTTCAAGTA
ACACCAGNTGTAACAAATACATTTACTTGCGNTGATCAAACTTCTTCACATGCANTCGGT
TCTGATTGGAGTGGAGTTTGTAAAATCANTGCCACACCCGCACCAACAGTTACACCAACA
GTTACACCAACAGTTACACCAACAGTAACACCAACACCAACAAATACACCAAATCCAACC
CCATCCCAAACATCAACAACAACTGGTTCTGCTTCAACTGTTGTTGCAAGTTTATCANTC
ATTATTT
Length of 3' end seq. 547
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -