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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHS429
Library CH
(Link to library)
Clone ID CHS429
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16419-1
Original site URL
Representative seq. ID CHS429P
(Link to Original site)
Representative DNA sequence 
>CHS429 (CHS429Q) /CSM/CH/CHS4-B/CHS429Q.Seq.d/
AATTCTTATTNAATTTTATTTTTAATCAATTCAAATTAAAAAAAAAACAATAACTAACTA
AGTTAAAAATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTA
TGATATTCCTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAANAAN
AATGTCATAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGTGGTGAACAAA
AATGGGCTTGCTCTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATANAA
ATTCATCCAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTA
TTAAAATTACAACTCCAACCGAGTATTCTTGTTCTGATGCCAANATTAAANAATGTGCCT
TATTAGGTAAATCATGTAGTTTCCAAAANAACTCTTGTTCAAATCCAACTTCCTGTTGCC
CAGGTGAATCAATXXXXXXXXXXTNTTTAAGATGTCCACCNAATTCATGAATGTAGATTT
AATGACCAAGGTCATCAATGCTGTGTAAAGGTTCACCATGATAGATGTTCATTAAGATGT
CCACATGGCCATGAATGTAAAGTTGATCACAATGGTAAAGAATGTTGCGTCAGATCCCAT
AGACCACCACCACCAGAAGTTTGTTCATTAAGATGCCCACCAAAACATGAATGTAAATTT
GATGATCATGGTAAAAAATGTTGTGTAAAGATTCATTGTGATGAAGTTTGTGATTTAGAT
TGTGGTAGAGGTTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGTGTCCGTTCA
GAAAGACCACACCCACCACAACATGAAAAATGTAATAAGAGATGCCCACCAGGCCATGAA
TGTAAAGTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAA
TGCTCTTTAAGATGTCCACCAAGACATGAATGTAGAGTCAACCACTTTGGTGAAGAATGT
TGTGTTAAAGTTCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCATGAATGTAAA
GTTGATCAACATGGAGAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTCA
TTAAGATGTCCACCAAAACATGAATGTAGAATCAATCACTTTGGTGAAGAATGCTGTGTT
AAAAGTAGAAATGATTGTTTAACTTGTGAAGACCTAAACTGTGAAAGAAAAGGTTTACAT
TGTGCCATGAAAACTGTACCAATTGATAAAGAAAATTGTTGGAAAAAGTNACAAGTACGT
TACTCCAA
sequence update 2002.10.25
Translated Amino Acid sequence 
flxnfifnqfklkkkq*ltklkikk**RMLFLKNIGVFFMIFLVSKSYATDCNKITNEXX
CHKSSECIVINYTPCCGEQKWACSKGTFDTCTYENSCYXNSSNNQVVEVSNKCFNLDGFI
KITTPTEYSCSDAXIKXCALLGKSCSFQXNSCSNPTSCCPGES---

---FKMSTXFMNVDLMTKVINAV*rftmidvh*dvhmamnvklitmvknvasdpidhhhq
kfvh*dahqnmnvnlmimvknvv*rfivmkfvi*ivvevlnvkldmmvqnvvsvqkdhth
hnmknvirdahqamnvklinmeknvallpidhhqnal*dvhqdmnvesttlvknvvlkft
tinvh*dvhqamnvklinmeknvallpidhhqnah*dvhqnmnvesitlvknavlkvemi
v*lvkt*tvkekvyivp*klyqlikkivgksxkyvtp
Translated Amino Acid sequence (All Frames) 
Frame A:
nsyxilflinsn*kknnn*ls*k*knnkgccf*kileyfl*yslyqnpmrrivikllmxx
nvinlrnvll*iihhvvvnknglalkvhlilvhmkivvixihpitkllkyqinasilmdl
lklqlqpsilvlmpxlxnvpy*vnhvvskxtlvqiqlpvaqvnq---

---xlrcppns*m*i**prssmlckgsp**mfikmstwp*m*s*sqw*rmlrqip*tttt
rslfikmptkt*m*i**sw*kmlckdsl**sl*frlw*rf*m*n*t*wfkmlcpfrkttp
ttt*km**emptrp*m*s*stwkrmlrccp*tttkmlfkmstkt*m*sqplw*rmlc*ss
pr*mfiemstrp*m*s*stwrrmlrccp*tttkmlikmstkt*m*nqslw*rmlc*k*k*
lfnl*rpkl*kkrftlchenctn**rkllekvtstllq

Frame B:
ilixfyf*siqikkktitn*vknkkiikdvvskkywsifydipcikilcdgl**ny**xx
ms*ifgmycyklytmlw*tkmgll*ryi*ylyi*k*llxkfiq*psc*sik*mlqs*wiy
*nynsnrvflf*cqx*xmclir*im*fpkxllfksnfllpr*in---

---x*dvhxihecrfndqghqccvkvhhdrcslrcphgheckvdhngkeccvrshrpppp
evcslrcppkheckfddhgkkccvkihcdevcdldcgrgfeckirhdgskccvrserphp
pqhekcnkrcppgheckvdqhgkeccvvahrpppkcslrcpprhecrvnhfgeeccvkvh
hdkcslrcppgheckvdqhgeeccvvahrpppkcslrcppkhecrinhfgeeccvksrnd
cltcedlncerkglhcamktvpidkencwkkxqvrys

Frame C:
flxnfifnqfklkkkq*ltklkikk**RMLFLKNIGVFFMIFLVSKSYATDCNKITNEXX
CHKSSECIVINYTPCCGEQKWACSKGTFDTCTYENSCYXNSSNNQVVEVSNKCFNLDGFI
KITTPTEYSCSDAXIKXCALLGKSCSFQXNSCSNPTSCCPGES---

---FKMSTXFMNVDLMTKVINAV*rftmidvh*dvhmamnvklitmvknvasdpidhhhq
kfvh*dahqnmnvnlmimvknvv*rfivmkfvi*ivvevlnvkldmmvqnvvsvqkdhth
hnmknvirdahqamnvklinmeknvallpidhhqnal*dvhqdmnvesttlvknvvlkft
tinvh*dvhqamnvklinmeknvallpidhhqnah*dvhqnmnvesitlvknavlkvemi
v*lvkt*tvkekvyivp*klyqlikkivgksxkyvtp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS429 (CHS429Q) /CSM/CH/CHS4-B/CHS429Q.Seq.d/                       2367   0.0
CHF268 (CHF268Q) /CSM/CH/CHF2-C/CHF268Q.Seq.d/                       1580   0.0
AHL722 (AHL722Q) /CSM/AH/AHL7-A/AHL722Q.Seq.d/                       1580   0.0
AHD145 (AHD145Q) /CSM/AH/AHD1-B/AHD145Q.Seq.d/                       1580   0.0
CHL140 (CHL140Q) /CSM/CH/CHL1-B/CHL140Q.Seq.d/                       1578   0.0
AHE263 (AHE263Q) /CSM/AH/AHE2-C/AHE263Q.Seq.d/                       1578   0.0
CHL853 (CHL853Q) /CSM/CH/CHL8-C/CHL853Q.Seq.d/                       1572   0.0
AHN244 (AHN244Q) /CSM/AH/AHN2-B/AHN244Q.Seq.d/                       1572   0.0
AHD377 (AHD377Q) /CSM/AH/AHD3-D/AHD377Q.Seq.d/                       1572   0.0
CHL174 (CHL174Q) /CSM/CH/CHL1-D/CHL174Q.Seq.d/                       1566   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U25144|U25144.1 Dictyostelium discoideum spore coat protein SP87 (PspD) gene, complete cds.  1532   0.0    2
AC117267|AC117267.2 Dictyostelium discoideum chromosome 2 map 5836255-5862024 strain AX4, complete sequence.  1532   0.0    2
M26239|M26239.1 D.discoideum spore coat protein SP60 gene, complete cds.    58   4e-14  4
X51892|X51892.1 Dictyostelium discoideum SP60 gene for spore coat protein.    58   1e-13  4
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    58   9e-08  2
X52105|X52105.1 Dictyostelium discoideum SP60 gene for spore coat protein.    42   4e-04  2
AC141955|AC141955.2 Rattus norvegicus clone CH230-418P10, WORKING DRAFT SEQUENCE, 29 unordered pieces.    48   0.20   3
AC162511|AC162511.3 Bos taurus clone CH240-113F1, *** SEQUENCING IN PROGRESS ***, 45 unordered pieces.    46   0.36   5
AC127160|AC127160.3 Rattus norvegicus clone CH230-460D14, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    48   0.48   1
AL161543|AL161543.2 Arabidopsis thaliana DNA chromosome 4, contig fragment No. 43.    42   1.1    6
dna update 2005.11.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...   622   e-177
(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...   202   2e-50
X52105_1(X52105|pid:none) Dictyostelium discoideum SP60 gene for...   120   8e-26
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;        91   5e-24
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...    90   8e-23
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    90   2e-16
(Q6TU45) RecName: Full=Probable spore coat protein sigD; AltName...    86   3e-15
(P54704) RecName: Full=Spore coat protein SP85; AltName: Full=Ce...    73   2e-11
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    70   2e-10
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    70   2e-10
protein update 2009. 4.13
PSORT 

 psg: 1.02  gvh: 0.68  alm: 0.49  top: 0.33  tms: 0.00  mit: 0.38  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: extracellular, including cell wall
	 12.0 %: mitochondrial
	  8.0 %: cytoplasmic
	  8.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: Golgi
	  4.0 %: vacuolar

>> prediction for CHS429 is exc
5' end seq. ID CHS429F
5' end seq. 
>CHS429F.Seq
AATTCTTATTNAATTTTATTTTTAATCAATTCAAATTAAAAAAAAAACAATAACTAACTA
AGTTAAAAATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTA
TGATATTCCTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAANAAN
AATGTCATAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGTGGTGAACAAA
AATGGGCTTGCTCTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATANAA
ATTCATCCAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTA
TTAAAATTACAACTCCAACCGAGTATTCTTGTTCTGATGCCAANATTAAANAATGTGCCT
TATTAGGTAAATCATGTAGTTTCCAAAANAACTCTTGTTCAAATCCAACTTCCTGTTGCC
CAGGTGAATCAATNNNNNNNNNN
Length of 5' end seq. 503
3' end seq. ID CHS429Z
3' end seq. 
>CHS429Z.Seq
NNNNNNNNNNTNTTTAAGATGTCCACCNAATTCATGAATGTAGATTTAATGACCAAGGTC
ATCAATGCTGTGTAAAGGTTCACCATGATAGATGTTCATTAAGATGTCCACATGGCCATG
AATGTAAAGTTGATCACAATGGTAAAGAATGTTGCGTCAGATCCCATAGACCACCACCAC
CAGAAGTTTGTTCATTAAGATGCCCACCAAAACATGAATGTAAATTTGATGATCATGGTA
AAAAATGTTGTGTAAAGATTCATTGTGATGAAGTTTGTGATTTAGATTGTGGTAGAGGTT
TTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGTGTCCGTTCAGAAAGACCACACC
CACCACAACATGAAAAATGTAATAAGAGATGCCCACCAGGCCATGAATGTAAAGTTGATC
AACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTCTTTAAGAT
GTCCACCAAGACATGAATGTAGAGTCAACCACTTTGGTGAAGAATGTTGTGTTAAAGTTC
ACCACGATAAATGTTCATTGAGATGTCCACCAGGCCATGAATGTAAAGTTGATCAACATG
GAGAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTCATTAAGATGTCCAC
CAAAACATGAATGTAGAATCAATCACTTTGGTGAAGAATGCTGTGTTAAAAGTAGAAATG
ATTGTTTAACTTGTGAAGACCTAAACTGTGAAAGAAAAGGTTTACATTGTGCCATGAAAA
CTGTACCAATTGATAAAGAAAATTGTTGGAAAAAGTNACAAGTACGTTACTCCAA
Length of 3' end seq. 835
Connected seq. ID CHS429P
Connected seq. 
>CHS429P.Seq
AATTCTTATTNAATTTTATTTTTAATCAATTCAAATTAAAAAAAAAACAATAACTAACTA
AGTTAAAAATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTA
TGATATTCCTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAANAAN
AATGTCATAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGTGGTGAACAAA
AATGGGCTTGCTCTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATANAA
ATTCATCCAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTA
TTAAAATTACAACTCCAACCGAGTATTCTTGTTCTGATGCCAANATTAAANAATGTGCCT
TATTAGGTAAATCATGTAGTTTCCAAAANAACTCTTGTTCAAATCCAACTTCCTGTTGCC
CAGGTGAATCAAT----------TNTTTAAGATGTCCACCNAATTCATGAATGTAGATTT
AATGACCAAGGTCATCAATGCTGTGTAAAGGTTCACCATGATAGATGTTCATTAAGATGT
CCACATGGCCATGAATGTAAAGTTGATCACAATGGTAAAGAATGTTGCGTCAGATCCCAT
AGACCACCACCACCAGAAGTTTGTTCATTAAGATGCCCACCAAAACATGAATGTAAATTT
GATGATCATGGTAAAAAATGTTGTGTAAAGATTCATTGTGATGAAGTTTGTGATTTAGAT
TGTGGTAGAGGTTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGTGTCCGTTCA
GAAAGACCACACCCACCACAACATGAAAAATGTAATAAGAGATGCCCACCAGGCCATGAA
TGTAAAGTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAA
TGCTCTTTAAGATGTCCACCAAGACATGAATGTAGAGTCAACCACTTTGGTGAAGAATGT
TGTGTTAAAGTTCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCATGAATGTAAA
GTTGATCAACATGGAGAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTCA
TTAAGATGTCCACCAAAACATGAATGTAGAATCAATCACTTTGGTGAAGAATGCTGTGTT
AAAAGTAGAAATGATTGTTTAACTTGTGAAGACCTAAACTGTGAAAGAAAAGGTTTACAT
TGTGCCATGAAAACTGTACCAATTGATAAAGAAAATTGTTGGAAAAAGTNACAAGTACGT
TACTCCAA
Length of connected seq. 1318
Full length Seq ID -
Full length Seq. -
Length of full length seq. -