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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHS432
Library CH
(Link to library)
Clone ID CHS432
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15902-1
Original site URL
Representative seq. ID CHS432P
(Link to Original site)
Representative DNA sequence 
>CHS432 (CHS432Q) /CSM/CH/CHS4-B/CHS432Q.Seq.d/
TTGGCCTACTGGAAAAAAAAAAGCCTATAATAATAATAATAATAGTAATAATAATAATAA
TAATAATAATTATAATAATAATAATTATAATAATTATAATAATAAAATAAATTTATACAA
AAATGACATATACTGATAGTCGATATAATACTTTAAATAGAGGTAGCAAGAAAATGGACC
CTCCATTCATTACTCAAGAAGTATTANCAAGTGCAAAAATATCTAAAAAAGATAATATAT
TAATCAATGCAAATGCTGAGGATGGTAGTGTTCATACATTCTCTGAANAANAAAAAGTTG
CTTATTCAAAATTTATAACAGAAAGATTGATAGATCAAGAGGATGTTTTATCAAATTATG
TTCCAATTGACCCATCAACTGATCAATTATTTGTAAATTGTACAGATGGNGTTTTACTAA
ATAAATTAATGGAATCATTATTTTTAACACAAGTTAATTTAAAAGGACTATGTATAAAAA
ATAAAATGAATCCATTTGAAATGATTCAAAATCAAAATATAGTAATTAAAAATGCAATTG
AAGTTGGTTGTATTATTGTAAATATTGGNGXXXXXXXXXXAACATTTGAACAATTGATGG
CAAATAATGATGTAGTATCTATTTTCAATTCAAGATGTCTTCAATTGGTTGAGCTTGGTA
ATGCATTCTTAGAACGTATAACAAAATCAATTGAACAACTACCAGTTCAATTACGTTATC
TTTGTAGAATAATTGCAGAGAAATCAAAATATGCTTTTCCAACCAATAATACCAACAATC
ACCACAATGGCGGTGGTATTTCAAGTGATGAAAGTGGTAGTACCACAGCTAATATAACTT
TAAATAATAATCGTTCAAAACAATTACCAGAGAATCGTGAAAAGTATATTTCTTACTTTT
TAATTATTTATCGTTTCATTATTCCAATTATTTCATCACCAGATCTTTTAAATGAATTAG
TATTACTTCAACATCCATACATTACAAAACAAAGTCGTTTCAATCTATCACAATTATCAC
GTTTAATTCAATCGGTTTTCTCTGAAAAACCTTGGATGAATTATTTCCCAACTGTTTATC
TCAATAAATGGATCATTCAAAATTCATCATCCGCTGATAAATTCCTTTGTCAAGTGGCAC
AAGTACCAGATTTAGATGAATTAATTCACTATAGCGAATATAATGAAATCATGTTGGAAA
ATAATATTAAATTTTGTTCAGTAATTAATATCTCTGAGATCATTTGGTTACATGAAACTA
TTCTTTCAAACTTTTATGATCTGAANATATTGGTACATGAAAGAGGTCNCNAGAAATCAA
TTCAAAATTTAGCAGAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
gllekkkpiiiiiiviiiiiiiiiiiiiiiiiiik*IYTKMTYTDSRYNTLNRGSKKMDP
PFITQEVLXSAKISKKDNILINANAEDGSVHTFSEXXKVAYSKFITERLIDQEDVLSNYV
PIDPSTDQLFVNCTDGVLLNKLMESLFLTQVNLKGLCIKNKMNPFEMIQNQNIVIKNAIE
VGCIIVNIG---

---TFEQLMANNDVVSIFNSRCLQLVELGNAFLERITKSIEQLPVQLRYLCRIIAEKSKY
AFPTNNTNNHHNGGGISSDESGSTTANITLNNNRSKQLPENREKYISYFLIIYRFIIPII
SSPDLLNELVLLQHPYITKQSRFNLSQLSRLIQSVFSEKPWMNYFPTVYLNKWIIQNSSS
ADKFLCQVAQVPDLDELIHYSEYNEIMLENNIKFCSVINISEIIWLHETILSNFYDLXIL
VHERGXXKSIQNLAE
Translated Amino Acid sequence (All Frames) 
Frame A:
laywkkksl**************l****l**l***nkfiqk*hilivdiil*ievarkwt
lhsllkkyxqvqkylkkiiy*smqmlrmvvfihslxxkklliqnl*qkd**ikrmfyqim
fqlthqlinyl*ivqmxfy*in*wnhyf*hkli*kdyv*kik*ihlk*fkiki**lkmql
klvvll*ilx---

---ni*tidgk**csiyfqfkmssig*aw*cilrtynkin*tttssitlsl*nncreiki
cfsnq*yqqspqwrwyfk**kw*yhs*ynfk**sfktitres*kvyfllfnylsfhysny
fitrsfk*isitstsihyktksfqsitiitfnsigfl*ktldelfpnclsq*mdhskfii
r**iplssgtstrfr*insl*ri**nhvgk*y*ilfsn*yl*dhlvt*nysfkll*sexi
gt*krsxeinskfsr

Frame B:
wptgkkkaynnnnnsnnnnnnnnynnnnynnynnkinlykndiy**si*yfk*r*qengp
sihysrsixkckni*kr*yinqckc*gw*csyil*xxksclfkiynrkidrsrgcfiklc
sn*pin*siicklyrwxftk*ingiiifnts*fkrtmykk*nesi*ndskskysn*kcn*
swlyyckyw---

---TFEQLMANNDVVSIFNSRCLQLVELGNAFLERITKSIEQLPVQLRYLCRIIAEKSKY
AFPTNNTNNHHNGGGISSDESGSTTANITLNNNRSKQLPENREKYISYFLIIYRFIIPII
SSPDLLNELVLLQHPYITKQSRFNLSQLSRLIQSVFSEKPWMNYFPTVYLNKWIIQNSSS
ADKFLCQVAQVPDLDELIHYSEYNEIMLENNIKFCSVINISEIIWLHETILSNFYDLXIL
VHERGXXKSIQNLAE

Frame C:
gllekkkpiiiiiiviiiiiiiiiiiiiiiiiiik*IYTKMTYTDSRYNTLNRGSKKMDP
PFITQEVLXSAKISKKDNILINANAEDGSVHTFSEXXKVAYSKFITERLIDQEDVLSNYV
PIDPSTDQLFVNCTDGVLLNKLMESLFLTQVNLKGLCIKNKMNPFEMIQNQNIVIKNAIE
VGCIIVNIG---

---hlnn*wqimm*ylfsiqdvfnwlslvmhs*nv*qnqlnnyqfnyvifve*lqrnqnm
lfqpiiptittmavvfqvmkvvvpqli*l*iiivqnnyqrivksifltf*lfivslfqlf
hhqif*mn*yyfnihtlqnkvvsiyhnyhv*fnrfslknlg*iisqlfisingsfkihhp
linsfvkwhkyqi*mn*ftianimkscwkiilnfvq*lislrsfgymklffqtfmi*xyw
ymkevxrnqfki*qk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS432 (CHS432Q) /CSM/CH/CHS4-B/CHS432Q.Seq.d/                       2323   0.0
CHD696 (CHD696Q) /CSM/CH/CHD6-D/CHD696Q.Seq.d/                       1011   0.0
FC-BB02 (FC-BB02Q) /CSM/FC/FC-BB/FC-BB02Q.Seq.d/                      922   0.0
SHI787 (SHI787Q) /CSM/SH/SHI7-D/SHI787Q.Seq.d/                        866   0.0
CHC529 (CHC529Q) /CSM/CH/CHC5-B/CHC529Q.Seq.d/                        759   0.0
CHK606 (CHK606Q) /CSM/CH/CHK6-A/CHK606Q.Seq.d/                        672   0.0
CHF723 (CHF723Q) /CSM/CH/CHF7-A/CHF723Q.Seq.d/                        424   e-117
CFD158 (CFD158Q) /CSM/CF/CFD1-C/CFD158Q.Seq.d/                        373   e-102
SHH130 (SHH130Q) /CSM/SH/SHH1-B/SHH130Q.Seq.d/                        218   2e-55
VHD169 (VHD169Q) /CSM/VH/VHD1-C/VHD169Q.Seq.d/                         44   0.005
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116551|AC116551.2 Dictyostelium discoideum chromosome 2 map complement(581327-427576) strain AX4, complete sequence.  1392   0.0    3
CJ438186|CJ438186.1 Macaca fascicularis mRNA, clone: QccE-15390, 5' end, expressed in cerebellum cortex.    50   0.038  2
AE017308|AE017308.1 Mycoplasma mobile 163K complete genome.        34   0.066  20
AX347137|AX347137.1 Sequence 2208 from Patent WO0200928.           34   0.069  5
CJ480078|CJ480078.1 Macaca fascicularis mRNA, clone: QtrA-15516, 5' end, expressed in brain temporal lobe.    44   0.11   3
AX059533|AX059533.1 Sequence 266 from Patent WO0055325.            36   0.12   6
DD000848|DD000848.1 PLANT ARTIFICIAL CHROMOSOME COMPOSITIONS AND METHODS.    36   0.12   6
CT027680|CT027680.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-216L5.    50   0.12   1
AC137466|AC137466.1 Rattus norvegicus clone CH230-unknown, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    50   0.12   1
AC132958|AC132958.3 Rattus norvegicus clone CH230-402I22, WORKING DRAFT SEQUENCE, 1 ordered piece.    50   0.12   1
dna update 2005.11.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC167378_1(BC167378|pid:none) Xenopus tropicalis hypothetical pr...    70   2e-10
(Q6P698) RecName: Full=Plastin-2; AltName: Full=L-plastin; AltNa...    69   4e-10
AM459664_1(AM459664|pid:none) Vitis vinifera contig VV78X045040....    67   1e-09
(A6H742) RecName: Full=Plastin-1;  &BC146105_1(BC146105|pid:none)      67   1e-09
AM600681_1(AM600681|pid:none) Oncorhynchus mykiss partial mRNA f...    67   2e-09
CR858800_1(CR858800|pid:none) Pongo abelii mRNA; cDNA DKFZp459F2...    66   2e-09
BC031083_1(BC031083|pid:none) Homo sapiens plastin 1 (I isoform)...    66   2e-09
(Q14651) RecName: Full=Plastin-1; AltName: Full=Intestine-specif...    66   2e-09
A84461(A84461) probable fimbrin [imported] - Arabidopsis thalian...    66   2e-09
AK290316_1(AK290316|pid:none) Homo sapiens cDNA FLJ78068 complet...    66   2e-09
protein update 2009. 4.13
PSORT 

 psg: 0.58  gvh: 0.30  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall
	  4.0 %: endoplasmic reticulum

>> prediction for CHS432 is nuc
5' end seq. ID CHS432F
5' end seq. 
>CHS432F.Seq
TTGGCCTACTGGAAAAAAAAAAGCCTATAATAATAATAATAATAGTAATAATAATAATAA
TAATAATAATTATAATAATAATAATTATAATAATTATAATAATAAAATAAATTTATACAA
AAATGACATATACTGATAGTCGATATAATACTTTAAATAGAGGTAGCAAGAAAATGGACC
CTCCATTCATTACTCAAGAAGTATTANCAAGTGCAAAAATATCTAAAAAAGATAATATAT
TAATCAATGCAAATGCTGAGGATGGTAGTGTTCATACATTCTCTGAANAANAAAAAGTTG
CTTATTCAAAATTTATAACAGAAAGATTGATAGATCAAGAGGATGTTTTATCAAATTATG
TTCCAATTGACCCATCAACTGATCAATTATTTGTAAATTGTACAGATGGNGTTTTACTAA
ATAAATTAATGGAATCATTATTTTTAACACAAGTTAATTTAAAAGGACTATGTATAAAAA
ATAAAATGAATCCATTTGAAATGATTCAAAATCAAAATATAGTAATTAAAAATGCAATTG
AAGTTGGTTGTATTATTGTAAATATTGGNGNNNNNNNNNN
Length of 5' end seq. 580
3' end seq. ID CHS432Z
3' end seq. 
>CHS432Z.Seq
NNNNNNNNNNAACATTTGAACAATTGATGGCAAATAATGATGTAGTATCTATTTTCAATT
CAAGATGTCTTCAATTGGTTGAGCTTGGTAATGCATTCTTAGAACGTATAACAAAATCAA
TTGAACAACTACCAGTTCAATTACGTTATCTTTGTAGAATAATTGCAGAGAAATCAAAAT
ATGCTTTTCCAACCAATAATACCAACAATCACCACAATGGCGGTGGTATTTCAAGTGATG
AAAGTGGTAGTACCACAGCTAATATAACTTTAAATAATAATCGTTCAAAACAATTACCAG
AGAATCGTGAAAAGTATATTTCTTACTTTTTAATTATTTATCGTTTCATTATTCCAATTA
TTTCATCACCAGATCTTTTAAATGAATTAGTATTACTTCAACATCCATACATTACAAAAC
AAAGTCGTTTCAATCTATCACAATTATCACGTTTAATTCAATCGGTTTTCTCTGAAAAAC
CTTGGATGAATTATTTCCCAACTGTTTATCTCAATAAATGGATCATTCAAAATTCATCAT
CCGCTGATAAATTCCTTTGTCAAGTGGCACAAGTACCAGATTTAGATGAATTAATTCACT
ATAGCGAATATAATGAAATCATGTTGGAAAATAATATTAAATTTTGTTCAGTAATTAATA
TCTCTGAGATCATTTGGTTACATGAAACTATTCTTTCAAACTTTTATGATCTGAANATAT
TGGTACATGAAAGAGGTCNCNAGAAATCAATTCAAAATTTAGCAGAAA
Length of 3' end seq. 768
Connected seq. ID CHS432P
Connected seq. 
>CHS432P.Seq
TTGGCCTACTGGAAAAAAAAAAGCCTATAATAATAATAATAATAGTAATAATAATAATAA
TAATAATAATTATAATAATAATAATTATAATAATTATAATAATAAAATAAATTTATACAA
AAATGACATATACTGATAGTCGATATAATACTTTAAATAGAGGTAGCAAGAAAATGGACC
CTCCATTCATTACTCAAGAAGTATTANCAAGTGCAAAAATATCTAAAAAAGATAATATAT
TAATCAATGCAAATGCTGAGGATGGTAGTGTTCATACATTCTCTGAANAANAAAAAGTTG
CTTATTCAAAATTTATAACAGAAAGATTGATAGATCAAGAGGATGTTTTATCAAATTATG
TTCCAATTGACCCATCAACTGATCAATTATTTGTAAATTGTACAGATGGNGTTTTACTAA
ATAAATTAATGGAATCATTATTTTTAACACAAGTTAATTTAAAAGGACTATGTATAAAAA
ATAAAATGAATCCATTTGAAATGATTCAAAATCAAAATATAGTAATTAAAAATGCAATTG
AAGTTGGTTGTATTATTGTAAATATTGGNG----------AACATTTGAACAATTGATGG
CAAATAATGATGTAGTATCTATTTTCAATTCAAGATGTCTTCAATTGGTTGAGCTTGGTA
ATGCATTCTTAGAACGTATAACAAAATCAATTGAACAACTACCAGTTCAATTACGTTATC
TTTGTAGAATAATTGCAGAGAAATCAAAATATGCTTTTCCAACCAATAATACCAACAATC
ACCACAATGGCGGTGGTATTTCAAGTGATGAAAGTGGTAGTACCACAGCTAATATAACTT
TAAATAATAATCGTTCAAAACAATTACCAGAGAATCGTGAAAAGTATATTTCTTACTTTT
TAATTATTTATCGTTTCATTATTCCAATTATTTCATCACCAGATCTTTTAAATGAATTAG
TATTACTTCAACATCCATACATTACAAAACAAAGTCGTTTCAATCTATCACAATTATCAC
GTTTAATTCAATCGGTTTTCTCTGAAAAACCTTGGATGAATTATTTCCCAACTGTTTATC
TCAATAAATGGATCATTCAAAATTCATCATCCGCTGATAAATTCCTTTGTCAAGTGGCAC
AAGTACCAGATTTAGATGAATTAATTCACTATAGCGAATATAATGAAATCATGTTGGAAA
ATAATATTAAATTTTGTTCAGTAATTAATATCTCTGAGATCATTTGGTTACATGAAACTA
TTCTTTCAAACTTTTATGATCTGAANATATTGGTACATGAAAGAGGTCNCNAGAAATCAA
TTCAAAATTTAGCAGAAA
Length of connected seq. 1328
Full length Seq ID -
Full length Seq. -
Length of full length seq. -