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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHS459
Library CH
(Link to library)
Clone ID CHS459
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID CHS459P
(Link to Original site)
Representative DNA sequence 
>CHS459 (CHS459Q) /CSM/CH/CHS4-C/CHS459Q.Seq.d/
ACTGTTGGCCTACTGGGTTAATTCAGTAGTTCAATTTAATTTATAATAATAATAATAGTA
TAAATATATAAAGATTAATTTTTTTGTATATAAATTTTTGTAAATAAATAAATAAATAAT
AATTTTTTTTTTAATTCACACACATACATATAAATATTAAATAATTAAATATTAAAAAAT
GTCATCAATTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGA
TCGTGTTGAAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATT
TACTGATACAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAAC
CAATACAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCA
AAGTGATATGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGNGATAAGCCACATAT
TCAAGTTGAATTCAAAGGNGAAACTAAAGTTTTCTCACCXXXXXXXXXXTGTAAGAAAAC
TCCAACCCTTCTCTACATACTCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAG
GTGAACGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTC
CACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTA
TCTTAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCA
ATGATAAAGGTCGTCTTTCAAAAGAAGAAATTAAAAAAATGGTTGCTGATGCAGAGAAAT
TCAAACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATT
ATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATT
CAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATC
AAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATC
CAATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTG
GTATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGA
ATTTTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAA
ATCAATAAAGCTTCTCATATA
sequence update 2002.10.25
Translated Amino Acid sequence 
llaywvnsvvqfnl*****ykyikinffvykfl*ink*iiifflihthtyky*iiky*KM
SSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPT
NTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFKGETKVFS---

---VRKLQPFSTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTF
DVDANGILNVSAEDKSTGNKQKITITNDKGRLSKEEIKKMVADAEKFKQQDEQQKDRVES
KNKLENYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKA
LEAVVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*
kiis**nq*sfsy
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllg*fsssi*fiiiiiv*iykd*ffci*ifvnk*innnfffnshtyi*ilnn*ilkn
vinwy*frynifmcwclak*sc*nyck*sr**nhsilcciy*yrtfnw*cckesscnesn
qysi*c*tfnws*i**qrssk*yetlaiqsyskgwx*atyss*iqrxn*sflt---

---ckktptllyil**strcinssl*r*tcydqr**fig*i*tfwyststkrctts*syi
*c*ckwylkcis*r*inw*qtkdynyq**rssfkrrn*kngc*creiqttr*ttkrsc*i
ke*igklcihc*kfn*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i*r*des
lrsscqsnhe*tlsrrryatrwwyarwyvk*ftkefkqqsrrirlteffrgkhlyqidfl
knnklvksiklli

Frame B:
llaywvnsvvqfnl*****ykyikinffvykfl*ink*iiifflihthtyky*iiky*KM
SSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPT
NTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFKGETKVFS---

---VRKLQPFSTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTF
DVDANGILNVSAEDKSTGNKQKITITNDKGRLSKEEIKKMVADAEKFKQQDEQQKDRVES
KNKLENYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKA
LEAVVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*
kiis**nq*sfsy

Frame C:
cwptgliq*fnliynnnnsini*rliflyinfck*ink**fff*fthihinik*lnikkc
hqlvli*vqhihvlvfgkmivlkllqmikvieplhlmlhlliqnv*lvmlqriklq*iqp
iqylmlnv*lvvnlvtkkfkvi*nighsklfqrmxishifklnskxklkfsh---

---*ensnpslhtliinqvy*fkfmkvnvl*pkiiiywvnlnflvfhqhqevyhklklhl
mlmqmvs*myqlkinqlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkmnnkkivlnq
rinwkimhsllkiqlkmkklqpkfqiqinqllnlklkvysng*nqikpqkrmnmkir*kp
*kqlsiqs*vnsikkevchkvvvcqvvcqmihqrvqttk*tn*idgifqrktslsnrflk
k**vskinkashi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS459 (CHS459Q) /CSM/CH/CHS4-C/CHS459Q.Seq.d/                       2165   0.0
VHK423 (VHK423Q) /CSM/VH/VHK4-A/VHK423Q.Seq.d/                       1447   0.0
VHP719 (VHP719Q) /CSM/VH/VHP7-A/VHP719Q.Seq.d/                       1443   0.0
CHM388 (CHM388Q) /CSM/CH/CHM3-D/CHM388Q.Seq.d/                       1443   0.0
CHF190 (CHF190Q) /CSM/CH/CHF1-D/CHF190Q.Seq.d/                       1443   0.0
VHP111 (VHP111Q) /CSM/VH/VHP1-A/VHP111Q.Seq.d/                       1441   0.0
VHK721 (VHK721Q) /CSM/VH/VHK7-A/VHK721Q.Seq.d/                       1441   0.0
VHJ320 (VHJ320Q) /CSM/VH/VHJ3-A/VHJ320Q.Seq.d/                       1441   0.0
VHI738 (VHI738Q) /CSM/VH/VHI7-B/VHI738Q.Seq.d/                       1441   0.0
VHH695 (VHH695Q) /CSM/VH/VHH6-D/VHH695Q.Seq.d/                       1441   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).   916   0.0    3
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.   916   0.0    3
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.   252   e-109  7
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   252   e-101  10
AF221541|AF221541.1 Cryptosporidium serpentis heat shock protein 70 (HSP70) gene, partial cds.   145   1e-94  9
M84652|M84652.1 Entamoeba heat shock protein (Hsp70) mRNA, complete cds.   172   1e-85  4
AY954892|AY954892.1 Cryptosporidium andersoni strain HenanZhzhca heat shock protein 70 gene, partial cds.   123   1e-83  9
DQ060426|DQ060426.1 Cryptosporidium andersoni strain zzca heat shock protein 70-like gene, partial sequence.   127   3e-83  8
AY954893|AY954893.1 Cryptosporidium andersoni strain changchunca heat shock protein 70 gene, partial cds.   129   6e-82  9
AY954894|AY954894.1 Cryptosporidium andersoni strain bjcm heat shock protein 70 gene, partial cds.   117   2e-81  9
dna update 2005.12. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   364   3e-99
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   364   3e-99
AF033852_1(AF033852|pid:none) Spinacia oleracea cytosolic heat s...   268   2e-70
FB790108_1(FB790108|pid:none) Sequence 9381 from Patent WO200803...   268   3e-70
FB790112_1(FB790112|pid:none) Sequence 9385 from Patent WO200803...   267   7e-70
(P22953) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   266   9e-70
S46302(S46302;S41402;JA0169;S36623) dnaK-type molecular chaperon...   266   9e-70
EF506932_1(EF506932|pid:none) Fasciola hepatica heat shock prote...   266   1e-69
X77199_1(X77199|pid:none) A.thaliana hsc70-1 gene.                    266   1e-69
EF506931_1(EF506931|pid:none) Fasciola gigantica heat shock prot...   265   2e-69
protein update 2009. 4.13
PSORT 

 psg: 0.87  gvh: 0.35  alm: 0.46  top: 0.27  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for CHS459 is cyt
5' end seq. ID CHS459F
5' end seq. 
>CHS459F.Seq
ACTGTTGGCCTACTGGGTTAATTCAGTAGTTCAATTTAATTTATAATAATAATAATAGTA
TAAATATATAAAGATTAATTTTTTTGTATATAAATTTTTGTAAATAAATAAATAAATAAT
AATTTTTTTTTTAATTCACACACATACATATAAATATTAAATAATTAAATATTAAAAAAT
GTCATCAATTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGA
TCGTGTTGAAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATT
TACTGATACAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAAC
CAATACAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCA
AAGTGATATGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGNGATAAGCCACATAT
TCAAGTTGAATTCAAAGGNGAAACTAAAGTTTTCTCACCNNNNNNNNNN
Length of 5' end seq. 529
3' end seq. ID CHS459Z
3' end seq. 
>CHS459Z.Seq
NNNNNNNNNNTGTAAGAAAACTCCAACCCTTCTCTACATACTCTGATAATCAACCAGGTG
TATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAATTTATTGGGTA
AATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACAT
TTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAACTGGTAACA
AACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTAAAAAAA
TGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCGTGTTGAAT
CAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAG
TTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTAC
TCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAG
CCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCAC
AAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAG
TAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCTCTATCAAATAGATTTCTT
AAAAAATAATAAGTTAGTAAAATCAATAAAGCTTCTCATATA
Length of 3' end seq. 762
Connected seq. ID CHS459P
Connected seq. 
>CHS459P.Seq
ACTGTTGGCCTACTGGGTTAATTCAGTAGTTCAATTTAATTTATAATAATAATAATAGTA
TAAATATATAAAGATTAATTTTTTTGTATATAAATTTTTGTAAATAAATAAATAAATAAT
AATTTTTTTTTTAATTCACACACATACATATAAATATTAAATAATTAAATATTAAAAAAT
GTCATCAATTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGA
TCGTGTTGAAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATT
TACTGATACAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAAC
CAATACAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCA
AAGTGATATGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGNGATAAGCCACATAT
TCAAGTTGAATTCAAAGGNGAAACTAAAGTTTTCTCACC----------TGTAAGAAAAC
TCCAACCCTTCTCTACATACTCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAG
GTGAACGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTC
CACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTA
TCTTAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCA
ATGATAAAGGTCGTCTTTCAAAAGAAGAAATTAAAAAAATGGTTGCTGATGCAGAGAAAT
TCAAACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATT
ATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATT
CAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATC
AAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATC
CAATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTG
GTATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGA
ATTTTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAA
ATCAATAAAGCTTCTCATATA
Length of connected seq. 1271
Full length Seq ID -
Full length Seq. -
Length of full length seq. -