>CHS465 (CHS465Q) /CSM/CH/CHS4-C/CHS465Q.Seq.d/
ACTGTTGGCCTACTGGTNTCCCTTAAAATAATAACAATAAAATANATTTTTACATTTATT
TTTATTTATTTCATATTTATTTTTGTTGGAAAAAAAAGAAAAAAAAAAAAAAAANGAAAA
AAAAAAXXXXXXXXXX

TVGLLVSLKIITIKXIFTFIFIYFIFIFVGKKRKKKKKXKK---

Frame A:
TVGLLVSLKIITIKXIFTFIFIYFIFIFVGKKRKKKKKXKK---


Frame B:
llaywxplk**q*nxflhlflfisylfllekkekkkkxekk---


Frame C:
cwptgxp*nnnnkixfyiyfylfhiyfcwkkkkkkkkxkkk---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHB682 (SHB682Q) /CSM/SH/SHB6-D/SHB682Q.Seq.d/                         82   2e-15
SHB591 (SHB591Q) /CSM/SH/SHB5-D/SHB591Q.Seq.d/                         82   2e-15
CHS465 (CHS465Q) /CSM/CH/CHS4-C/CHS465Q.Seq.d/                         82   2e-15
SHJ748 (SHJ748Q) /CSM/SH/SHJ7-B/SHJ748Q.Seq.d/                         76   1e-13
SHJ540 (SHJ540Q) /CSM/SH/SHJ5-B/SHJ540Q.Seq.d/                         76   1e-13
SHB849 (SHB849Q) /CSM/SH/SHB8-C/SHB849Q.Seq.d/                         76   1e-13
SHB750 (SHB750Q) /CSM/SH/SHB7-C/SHB750Q.Seq.d/                         76   1e-13
SHB705 (SHB705Q) /CSM/SH/SHB7-A/SHB705Q.Seq.d/                         76   1e-13
SHB636 (SHB636Q) /CSM/SH/SHB6-B/SHB636Q.Seq.d/                         76   1e-13
CHP720 (CHP720Q) /CSM/CH/CHP7-A/CHP720Q.Seq.d/                         76   1e-13

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CR385071|CR385071.6 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-245B7.    50   0.011  1
CR936281|CR936281.1 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    50   0.011  1
CJ465402|CJ465402.1 Macaca fascicularis mRNA, clone: QnpA-14490, 5' end, expressed in brain parietal lobe.    36   0.069  2
CJ447438|CJ447438.1 Macaca fascicularis mRNA, clone: QflA-12768, 5' end, expressed in brain frontal lobe.    36   0.074  2
CJ454626|CJ454626.1 Macaca fascicularis mRNA, clone: QflA-20373, 5' end, expressed in brain frontal lobe.    34   0.26   2
CJ455207|CJ455207.1 Macaca fascicularis mRNA, clone: QflA-20979, 5' end, expressed in brain frontal lobe.    34   0.27   2
CJ452719|CJ452719.1 Macaca fascicularis mRNA, clone: QflA-18385, 5' end, expressed in brain frontal lobe.    34   0.27   2
AC167463|AC167463.2 Mus musculus chromosome 18 clone RP23-378D22 map 18, *** SEQUENCING IN PROGRESS ***, 2 ordered pieces.    44   0.65   1
AC079010|AC079010.2 Homo sapiens chromosome 4 clone RP11-183A12 map 4, WORKING DRAFT SEQUENCE, 4 unordered pieces.    44   0.65   1
AP003949|AP003949.1 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 6, BAC clone:OJ1269_H07, WORKING DRAFT SEQUENCE, 1 ordered pieces.    44   0.65   1

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 79,053,402

 psg: 0.84  gvh: 0.43  alm: 0.22  top: 1.00  tms: 0.07  mit: 0.36  mip: 0.00
 nuc: 0.32  erl: 0.00  erm: 1.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.17  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 24.0 %: nuclear
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for CHS465 is cyt

>CHS465F.Seq
ACTGTTGGCCTACTGGTNTCCCTTAAAATAATAACAATAAAATANATTTTTACATTTATT
TTTATTTATTTCATATTTATTTTTGTTGGAAAAAAAAGAAAAAAAAAAAAAAAANGAAAA
AAAAAANNNNNNNNNN