NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHS477
Library CH
(Link to library)
Clone ID CHS477
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16333-1
Original site URL
Representative seq. ID CHS477P
(Link to Original site)
Representative DNA sequence 
>CHS477 (CHS477Q) /CSM/CH/CHS4-D/CHS477Q.Seq.d/
CTGTTGGCCTACTGGAATAATTTAATTTTTTTCAAAAAAATAATAATAATAATAATAAAA
ATAATAAAAAAAATAAATATTAATAAATATTATTTAAAAATTTTATTTATAGCAAAATAA
CTAATATATTGTAAAATAATTTTAGAATATTTAAAAAATATTCATATAATAATAAATAAA
AATGAAAGAATATAAAAAAATTATATTATATAGTTTTTTAGTTTTTTTAATTTTATGTTT
TGCAAAACCATCTCTTTCAGATGACCCTTTAACAACTTCAACAACAACAACAACAGCAAC
TGCACTACCAACGACATCGGCAGCATCAACAGCAAAGGTAACAACAACAACCACAACTAC
AGGATCACCAGCATCAACCACAACTACAGGAGCACCAACATCAACTACAGGAGCATCAAC
ATCAACTACAGGAGCACCATCATCATCATCTTCATCGAATGAAACAACTCCAACTGCAAC
ACCAACTAAAAAACCTGTAACACCAACACCATCATCAACATCATCATCATCATCATCGNC
TGAAGTAACATCATCCTCATCCGAATCATXXXXXXXXXXGAATATCCATACTATCAACTT
GGTCAATTATTAAATGGTGCTTCCGATCAGTATAACNCTGGTAATGTAAATTTCAAATCC
GTTAGTAAACTTTTTANAGGTTTTCCAACTATCGAGTATCAATTTATNCAATAATTACAA
AGCNATCATNTCAATGATGAATGGTACAATCGATACATNTCAATATGATNTTTTGAGATT
GAATTTNTTNTTCCTTCATGATCAATNTTATAATAATCCAAATCCAAGTATTGACTTTAG
TGAAGTTGCAAACTNTTTCTTTTTAGATNTTCAAATTTGTATTACCGTTCAAGTGGTTGG
TAATTTACCTTGTGTTGGTTTATCNCCATACTTGGGTGGTCAAGTTCCAAATGCAGTATG
GGTTGAACTTTTAATTCCTTTCTNTGATNTTCAAGTTTATCCAAATGCTNTTATCAGTTG
TATCNCTTTCCAAACTCAAGTACAAGATCNCCAAGGTGTTTTNTTTATTGGTGATATTTC
TGCTGGTAATTTTGTACCNCCATATAAAGTACTANAGGATGGTTCAAACGCTTTCAAATT
ATTATCAAATATTTATTTATTATTATTAATTTTTATAATTTCNTTATTATTTATCTAAAC
AAAAAAAAAACAAATAAATAAAAAACATATATATT
sequence update 2002.10.25
Translated Amino Acid sequence 
cwptgii*ffskk******k**kk*ilinii*kfyl*qnn*yivk*f*ni*kifi***IK
MKEYKKIILYSFLVFLILCFAKPSLSDDPLTTSTTTTTATALPTTSAASTAKVTTTTTTT
GSPASTTTTGAPTSTTGASTSTTGAPSSSSSSNETTPTATPTKKPVTPTPSSTSSSSSSX
EVTSSSSES---

---EYPYYQLGQLLNGASDQYNXGNVNFKSVSKLFXGFPTIEYQFXQ*lqsxhxndewyn
ryxsi*xfeiefxxps*sxl**sksky*l**scklflfrxsnlyyrssgw*ftlcwfixi
lgwssskcsmg*tfnsfl*xsslskcxyqlyxfpnsstrsprcfxyw*yfcw*fctxi*s
txgwfkrfqiiikylfiiinfynfxiiylnkkktnk*ktyi
Translated Amino Acid sequence (All Frames) 
Frame A:
llaywnnliffkkiiiiiikiikkininkyylkilfiak*liyckiileylknihiiink
neri*knyii*ffsffnfmfcktisfr*pfnnfnnnnnsncttndigsinskgnnnnhny
ritsinhnyrstninyrsininyrstiiiifie*nnsncntn*ktcntntiiniiiiiix
*sniiliri---

---EYPYYQLGQLLNGASDQYNXGNVNFKSVSKLFXGFPTIEYQFXQ*lqsxhxndewyn
ryxsi*xfeiefxxps*sxl**sksky*l**scklflfrxsnlyyrssgw*ftlcwfixi
lgwssskcsmg*tfnsfl*xsslskcxyqlyxfpnsstrsprcfxyw*yfcw*fctxi*s
txgwfkrfqiiikylfiiinfynfxiiylnkkktnk*ktyi

Frame B:
cwptgii*ffskk******k**kk*ilinii*kfyl*qnn*yivk*f*ni*kifi***IK
MKEYKKIILYSFLVFLILCFAKPSLSDDPLTTSTTTTTATALPTTSAASTAKVTTTTTTT
GSPASTTTTGAPTSTTGASTSTTGAPSSSSSSNETTPTATPTKKPVTPTPSSTSSSSSSX
EVTSSSSES---

---nihtinlvny*mvlpisitlvm*isnplvnflxvfqlssinlxnnykaixsmmngti
dtxqydxlrlnxxflhdqxynnpnpsidfsevanxffldxqicitvqvvgnlpcvglspy
lggqvpnavwvellipfxdxqvypnaxiscixfqtqvqdxqgvxfigdisagnfvppykv
lxdgsnafkllsniyllllifiisllfi*tkkkqinkkhiy

Frame C:
vglle*fnffqknnnnnnknnkknky**ilfknfiyskitnil*nnfrifkkysynnk*k
*knikklyyivf*ff*fyvlqnhlfqmtl*qlqqqqqqqlhyqrhrqhqqqr*qqqpqlq
dhqhqpqlqehqhqlqehqhqlqehhhhhlhrmkqlqlqhqlknl*hqhhhqhhhhhhrl
k*hhphpnh---

---isilstwsiikwcfrsv*xw*ckfqir**tfxrfsnyrvsiyxiitkxsxq**mvqs
ihxnmxf*d*ixxsfminxiiiqiqvltlvklqtxsf*xfkfvlpfkwlviylvlvyxht
wvvkfqmqyglnf*flsxxfkfiqmllsvvslsklkykixkvfxllvifllvilyxhiky
xrmvqtlsnyyqifiyyy*fl*fxyylskqkknk*ikniyi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS477 (CHS477Q) /CSM/CH/CHS4-D/CHS477Q.Seq.d/                        938   0.0
SHJ588 (SHJ588Q) /CSM/SH/SHJ5-D/SHJ588Q.Seq.d/                        914   0.0
CHS395 (CHS395Q) /CSM/CH/CHS3-D/CHS395Q.Seq.d/                        908   0.0
CHR283 (CHR283Q) /CSM/CH/CHR2-D/CHR283Q.Seq.d/                        906   0.0
CHI503 (CHI503Q) /CSM/CH/CHI5-A/CHI503Q.Seq.d/                        906   0.0
CHR237 (CHR237Q) /CSM/CH/CHR2-B/CHR237Q.Seq.d/                        904   0.0
CHS330 (CHS330Q) /CSM/CH/CHS3-B/CHS330Q.Seq.d/                        902   0.0
AHM471 (AHM471Q) /CSM/AH/AHM4-C/AHM471Q.Seq.d/                        902   0.0
AHM266 (AHM266Q) /CSM/AH/AHM2-C/AHM266Q.Seq.d/                        902   0.0
CHR809 (CHR809Q) /CSM/CH/CHR8-A/CHR809Q.Seq.d/                        898   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC114263|AC114263.2 Dictyostelium discoideum chromosome 2 map 215673-367476 strain AX4, complete sequence.    40   0.005  2
AC126867|AC126867.4 Rattus norvegicus clone CH230-49B18, WORKING DRAFT SEQUENCE, 1 ordered piece.    54   0.007  3
AC095285|AC095285.5 Rattus norvegicus clone CH230-11A8, WORKING DRAFT SEQUENCE, 1 ordered piece.    54   0.007  3
BP663205|BP663205.1 Arabidopsis thaliana cDNA clone:RAFL21-47-C10, 3' end.    38   0.019  2
DT505060|DT505060.1 WS01315.B21_O02 PTxD-IL-FL-A-4 Populus trichocarpa x Populus deltoides cDNA clone WS01315_O02 3', mRNA sequence.    38   0.085  2
BJ008043|BJ008043.1 Oryzias latipes cDNA clone:MF01SSA114H01 : 5' end, single read.    34   0.086  2
CN518273|CN518273.1 GQ0093.B3_H17 GQ009 Populus balsamifera subsp. trichocarpa x Populus deltoides cDNA clone GQ0093_H17 5', mRNA sequence.    38   0.088  2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    38   0.20   6
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    44   0.24   5
AY160096|AY160096.1 Dictyostelium discoideum nucleotide exchange factor RasGEF G (gefG) gene, complete cds.    38   0.35   3
dna update 2005.12. 1
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 921,125,599
protein update 2009. 4.13
PSORT 

 psg: 1.00  gvh: 0.82  alm: 0.55  top: 0.20  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: endoplasmic reticulum
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for CHS477 is exc
5' end seq. ID CHS477F
5' end seq. 
>CHS477F.Seq
CTGTTGGCCTACTGGAATAATTTAATTTTTTTCAAAAAAATAATAATAATAATAATAAAA
ATAATAAAAAAAATAAATATTAATAAATATTATTTAAAAATTTTATTTATAGCAAAATAA
CTAATATATTGTAAAATAATTTTAGAATATTTAAAAAATATTCATATAATAATAAATAAA
AATGAAAGAATATAAAAAAATTATATTATATAGTTTTTTAGTTTTTTTAATTTTATGTTT
TGCAAAACCATCTCTTTCAGATGACCCTTTAACAACTTCAACAACAACAACAACAGCAAC
TGCACTACCAACGACATCGGCAGCATCAACAGCAAAGGTAACAACAACAACCACAACTAC
AGGATCACCAGCATCAACCACAACTACAGGAGCACCAACATCAACTACAGGAGCATCAAC
ATCAACTACAGGAGCACCATCATCATCATCTTCATCGAATGAAACAACTCCAACTGCAAC
ACCAACTAAAAAACCTGTAACACCAACACCATCATCAACATCATCATCATCATCATCGNC
TGAAGTAACATCATCCTCATCCGAATCATNNNNNNNNNN
Length of 5' end seq. 579
3' end seq. ID CHS477Z
3' end seq. 
>CHS477Z.Seq
NNNNNNNNNNGAATATCCATACTATCAACTTGGTCAATTATTAAATGGTGCTTCCGATCA
GTATAACNCTGGTAATGTAAATTTCAAATCCGTTAGTAAACTTTTTANAGGTTTTCCAAC
TATCGAGTATCAATTTATNCAATAATTACAAAGCNATCATNTCAATGATGAATGGTACAA
TCGATACATNTCAATATGATNTTTTGAGATTGAATTTNTTNTTCCTTCATGATCAATNTT
ATAATAATCCAAATCCAAGTATTGACTTTAGTGAAGTTGCAAACTNTTTCTTTTTAGATN
TTCAAATTTGTATTACCGTTCAAGTGGTTGGTAATTTACCTTGTGTTGGTTTATCNCCAT
ACTTGGGTGGTCAAGTTCCAAATGCAGTATGGGTTGAACTTTTAATTCCTTTCTNTGATN
TTCAAGTTTATCCAAATGCTNTTATCAGTTGTATCNCTTTCCAAACTCAAGTACAAGATC
NCCAAGGTGTTTTNTTTATTGGTGATATTTCTGCTGGTAATTTTGTACCNCCATATAAAG
TACTANAGGATGGTTCAAACGCTTTCAAATTATTATCAAATATTTATTTATTATTATTAA
TTTTTATAATTTCNTTATTATTTATCTAAACAAAAAAAAAACAAATAAATAAAAAACATA
TATATT
Length of 3' end seq. 666
Connected seq. ID CHS477P
Connected seq. 
>CHS477P.Seq
CTGTTGGCCTACTGGAATAATTTAATTTTTTTCAAAAAAATAATAATAATAATAATAAAA
ATAATAAAAAAAATAAATATTAATAAATATTATTTAAAAATTTTATTTATAGCAAAATAA
CTAATATATTGTAAAATAATTTTAGAATATTTAAAAAATATTCATATAATAATAAATAAA
AATGAAAGAATATAAAAAAATTATATTATATAGTTTTTTAGTTTTTTTAATTTTATGTTT
TGCAAAACCATCTCTTTCAGATGACCCTTTAACAACTTCAACAACAACAACAACAGCAAC
TGCACTACCAACGACATCGGCAGCATCAACAGCAAAGGTAACAACAACAACCACAACTAC
AGGATCACCAGCATCAACCACAACTACAGGAGCACCAACATCAACTACAGGAGCATCAAC
ATCAACTACAGGAGCACCATCATCATCATCTTCATCGAATGAAACAACTCCAACTGCAAC
ACCAACTAAAAAACCTGTAACACCAACACCATCATCAACATCATCATCATCATCATCGNC
TGAAGTAACATCATCCTCATCCGAATCAT----------GAATATCCATACTATCAACTT
GGTCAATTATTAAATGGTGCTTCCGATCAGTATAACNCTGGTAATGTAAATTTCAAATCC
GTTAGTAAACTTTTTANAGGTTTTCCAACTATCGAGTATCAATTTATNCAATAATTACAA
AGCNATCATNTCAATGATGAATGGTACAATCGATACATNTCAATATGATNTTTTGAGATT
GAATTTNTTNTTCCTTCATGATCAATNTTATAATAATCCAAATCCAAGTATTGACTTTAG
TGAAGTTGCAAACTNTTTCTTTTTAGATNTTCAAATTTGTATTACCGTTCAAGTGGTTGG
TAATTTACCTTGTGTTGGTTTATCNCCATACTTGGGTGGTCAAGTTCCAAATGCAGTATG
GGTTGAACTTTTAATTCCTTTCTNTGATNTTCAAGTTTATCCAAATGCTNTTATCAGTTG
TATCNCTTTCCAAACTCAAGTACAAGATCNCCAAGGTGTTTTNTTTATTGGTGATATTTC
TGCTGGTAATTTTGTACCNCCATATAAAGTACTANAGGATGGTTCAAACGCTTTCAAATT
ATTATCAAATATTTATTTATTATTATTAATTTTTATAATTTCNTTATTATTTATCTAAAC
AAAAAAAAAACAAATAAATAAAAAACATATATATT
Length of connected seq. 1225
Full length Seq ID -
Full length Seq. -
Length of full length seq. -