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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
FC-AI21
Library FC
(Link to library)
Clone ID FC-AI21
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16254-1
Original site URL
Representative seq. ID FC-AI21Z
(Link to Original site)
Representative DNA sequence 
>FC-AI21 (FC-AI21Q) /CSM/FC/FC-AI/FC-AI21Q.Seq.d/
XXXXXXXXXXAGTTCTTTTAACCGATATGTCTTCATATGCTGATGCTCTTCGTGAGGTAT
CTGCTGCTCGTGAAGAAGTACCAGGTCGTCGTGGTTATCCAGGTTATATGTATACAGATC
TTTCAACCATTTACGAACGTGCTGGTCGTATTCAAGGTCGTAATGGTTCTATCACTCAAA
TTCCAATTTTGACTATGCCAAATGATGATATTACCCATCCAATTCCAGATTTAACTGGTT
ACATTACTGAAGGTCAAATTTTCATTGATCGTCAAATCAATAACAGACAAATCTACCCAC
CAATCAATGTATTACCATCACTTTCTCGTCTTATGAAATCCGCCATTGGTGACGACATGA
CCCGTGGCGATCACTCTGAAGTCTCAAATCAAATGTATGCCAACTATGCCATCGGTAAAG
ATGTCCAAGCTATGAAAGCTGTCGTTGGTGAGGAAGCACTTTCTTCTGAAGATAAACTCT
ACTTAGAATTTTTAGAACGTTTCGAATCTAGCTTTGTCGGTCAAAACCATTATGAAAATC
GTGATATCTTTAATTCATTGGATCTTGGTTGGTCTTTATTAAGAACTTTCCCAAGCAATC
TCCTCAAACGTATCACTGAAAAAACTATTAAACAATACTATTCACGTTCATCAAAGGGTA
CTGTAGATTCAACTAACTAAATTTTAAAAAGAAAAAAATATAAATC
sequence update 1996.12. 6
Translated Amino Acid sequence 
---VLLTDMSSYADALREVSAAREEVPGRRGYPGYMYTDLSTIYERAGRIQGRNGSITQI
PILTMPNDDITHPIPDLTGYITEGQIFIDRQINNRQIYPPINVLPSLSRLMKSAIGDDMT
RGDHSEVSNQMYANYAIGKDVQAMKAVVGEEALSSEDKLYLEFLERFESSFVGQNHYENR
DIFNSLDLGWSLLRTFPSNLLKRITEKTIKQYYSRSSKGTVDSTN*ilkrkkyk
Translated Amino Acid sequence (All Frames) 
Frame A:
---VLLTDMSSYADALREVSAAREEVPGRRGYPGYMYTDLSTIYERAGRIQGRNGSITQI
PILTMPNDDITHPIPDLTGYITEGQIFIDRQINNRQIYPPINVLPSLSRLMKSAIGDDMT
RGDHSEVSNQMYANYAIGKDVQAMKAVVGEEALSSEDKLYLEFLERFESSFVGQNHYENR
DIFNSLDLGWSLLRTFPSNLLKRITEKTIKQYYSRSSKGTVDSTN*ilkrkkyk


Frame B:
---ff*piclhmlmlfvrylllvkkyqvvvviqviciqifqpftnvlvvfkvvmvlslkf
qf*lcqmmilpiqfqi*lvtllkvkfslivksitdksthqsmyyhhflvl*npplvtt*p
vaitlksqikcmptmpsvkmskl*klslvrkhfllkinst*nf*nvsnlalsvktimkiv
islihwilvgly*elsqaissnvslkkllnntihvhqrvl*iqltkf*keknin


Frame C:
---sfnryvfic*css*giccs*rstrsswlsrlyvyrsfnhlrtcwsysrs*wfyhsns
nfdyak**yypsnsrfnwlhy*rsnfh*ssnq*qtnlptnqcititfssyeirhw*rhdp
wrsl*slksnvcqlchr*rcpsyescrw*gstff*r*tllrifrtfri*lcrskpl*ks*
yl*figswlvfiknfpkqspqtyh*kny*tilftfikgycrfn*lnfkkkki*i
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFK761 (SFK761Q) /CSM/SF/SFK7-C/SFK761Q.Seq.d/                       1338   0.0
FC-AI21 (FC-AI21Q) /CSM/FC/FC-AI/FC-AI21Q.Seq.d/                     1338   0.0
CHB733 (CHB733Q) /CSM/CH/CHB7-B/CHB733Q.Seq.d/                       1338   0.0
CHB350 (CHB350Q) /CSM/CH/CHB3-C/CHB350Q.Seq.d/                       1338   0.0
CFJ409 (CFJ409Q) /CSM/CF/CFJ4-A/CFJ409Q.Seq.d/                       1338   0.0
CFI803 (CFI803Q) /CSM/CF/CFI8-A/CFI803Q.Seq.d/                       1338   0.0
CFI571 (CFI571Q) /CSM/CF/CFI5-C/CFI571Q.Seq.d/                       1338   0.0
AFM777 (AFM777Q) /CSM/AF/AFM7-D/AFM777Q.Seq.d/                       1338   0.0
AFM386 (AFM386Q) /CSM/AF/AFM3-D/AFM386Q.Seq.d/                       1338   0.0
AFC794 (AFC794Q) /CSM/AF/AFC7-D/AFC794Q.Seq.d/                       1338   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116963|AC116963.1 Dictyostelium discoideum chromosome 2 map 4790445-4846595 strain AX4, *** SEQUENCING IN PROGRESS ***.  1338  0.0  1
AC117076|AC117076.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.  1338  0.0  1
U63317|U63317.1 Dictyostelium discoideum vacuolar H+ ATPase B subunit (dvacB) mRNA, partial cds.  1205  0.0  1
BI703972|BI703972.1 kt46e07.y3 Strongyloides ratti L1 pAMP1 v3 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to SW:VATB_CAEEL Q19626 PROBABLE VACUOLAR ATP SYNTHASE SUBUNIT B ;, mRNA sequence.   194  5e-70  2
BI703973|BI703973.1 kt46f03.y3 Strongyloides ratti L1 pAMP1 v3 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to SW:VATB_CAEEL Q19626 PROBABLE VACUOLAR ATP SYNTHASE SUBUNIT B ;, mRNA sequence.   194  5e-70  2
BI516063|BI516063.1 BB160021A20C05 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160021A20C05 5', mRNA sequence.   252  5e-63  1
AJ567912|AJ567912.1 Mucor circinelloides partial mRNA for vacuolar ATP synthase subunit  B (vasB gene).   222  8e-60  2
AZ535060|AZ535060.1 ENTBW36TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.   115  2e-55  4
AZ540793|AZ540793.1 ENTFK06TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.   115  3e-51  4
AY231438|AY231438.1 Suaeda maritima subsp. salsa vacuolar H(+)-ATPase subunit B (VHA-B) mRNA, complete cds.    90  8e-51  4
dna update 2003. 7.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q76NU1) RecName: Full=V-type proton ATPase subunit B;          ...   443   e-123
U63317_1(U63317|pid:none) Dictyostelium discoideum vacuolar H+ A...   417   e-115
(Q38681) RecName: Full=V-type proton ATPase subunit B 1;        ...   362   4e-99
AP002901_17(AP002901|pid:none) Oryza sativa Japonica Group genom...   360   3e-98
EF153483_1(EF153483|pid:none) Medicago truncatula vacuolar H+-AT...   358   1e-97
AK176408_1(AK176408|pid:none) Arabidopsis thaliana mRNA for vacu...   358   1e-97
EF114316_1(EF114316|pid:none) Kalidium foliatum vacuolar ATPase ...   358   1e-97
(P11574) RecName: Full=V-type proton ATPase subunit B;          ...   358   1e-97
AK222158_1(AK222158|pid:none) Arabidopsis thaliana mRNA for vacu...   358   1e-97
AL035540_7(AL035540|pid:none) Arabidopsis thaliana DNA chromosom...   358   1e-97
protein update 2009. 4.13
PSORT 

 psg: 0.75  gvh: 0.39  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for FC-AI21 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID FC-AI21Z
3' end seq. 
>FC-AI21Z.Seq
----------AGTTCTTTTAACCGATATGTCTTCATATGCTGATGCTCTTCGTGAGGTAT
CTGCTGCTCGTGAAGAAGTACCAGGTCGTCGTGGTTATCCAGGTTATATGTATACAGATC
TTTCAACCATTTACGAACGTGCTGGTCGTATTCAAGGTCGTAATGGTTCTATCACTCAAA
TTCCAATTTTGACTATGCCAAATGATGATATTACCCATCCAATTCCAGATTTAACTGGTT
ACATTACTGAAGGTCAAATTTTCATTGATCGTCAAATCAATAACAGACAAATCTACCCAC
CAATCAATGTATTACCATCACTTTCTCGTCTTATGAAATCCGCCATTGGTGACGACATGA
CCCGTGGCGATCACTCTGAAGTCTCAAATCAAATGTATGCCAACTATGCCATCGGTAAAG
ATGTCCAAGCTATGAAAGCTGTCGTTGGTGAGGAAGCACTTTCTTCTGAAGATAAACTCT
ACTTAGAATTTTTAGAACGTTTCGAATCTAGCTTTGTCGGTCAAAACCATTATGAAAATC
GTGATATCTTTAATTCATTGGATCTTGGTTGGTCTTTATTAAGAACTTTCCCAAGCAATC
TCCTCAAACGTATCACTGAAAAAACTATTAAACAATACTATTCACGTTCATCAAAGGGTA
CTGTAGATTCAACTAACTAAATTTTAAAAAGAAAAAAATATAAATC
Length of 3' end seq. 696
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -