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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
FC-BR21
Library FC
(Link to library)
Clone ID FC-BR21
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15384-1|Contig-U16443-1
Original site URL
Representative seq. ID FC-BR21P
(Link to Original site)
Representative DNA sequence 
>FC-BR21 (FC-BR21Q) /CSM/FC/FC-BR/FC-BR21Q.Seq.d/
GTTGGAGTGGAGTTGGCCGCAGAGATAGTGGACCATTTCAAAGGCAAGGAAGTGACAATC
GTGCATTCACAATCGAAATTAATGAACAGGTTCCCCAAGAAGACAATACGTTACACTGAG
GAATTCTTACAAAAGCGTGGTGTTAAATTGATTTATAATGAACGTGTTGTGGCTCATAGG
GGTCAAACATTCATAACCGATCAGGGTAGTGAGATCATTGCTGAGCAAGCGTTCCTTTGT
ACTGGTATCGCACCAAATTCAAATTTCATTAAAAACAGCTACCCCGATGCCATCTCTGAG
AATGGTTACATCAAAGCTAACGACCAACTCCAGATGGCCGGCACTACATTCTATCGTAAT
ATCTTTGTGTCTGGTGACGTCTTACATGTACGTGAAGAGAAGTTAGCCCAAACTGCTGAA
TGCACAGCTTCAATAGTGGTCAATAATATCAATGCAATGAAAGTCGTTGTGAACATAAAA
TGCAATCTTATAAACCTTTTGCAAACCTGTTTTAATAAGTCTTGGAAAATATTCTGCTAT
CTTTGTTTATAXXXXXXXXXXTAAAACTAAAAAAGAAAAATAAAAAAAAAAAAAAAAATG
GAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAA
sequence update 1998. 5.20
Translated Amino Acid sequence 
VGVELAAEIVDHFKGKEVTIVHSQSKLMNRFPKKTIRYTEEFLQKRGVKLIYNERVVAHR
GQTFITDQGSEIIAEQAFLCTGIAPNSNFIKNSYPDAISENGYIKANDQLQMAGTTFYRN
IFVSGDVLHVREEKLAQTAECTASIVVNNINAMKVVVNIKCNLINLLQTCFNKSWKIFCY
LCL---

---KLKKKNKKKKKNGIKKKKKKKKKKKKKKKKKKKKKKKKKK
Translated Amino Acid sequence (All Frames) 
Frame A:
VGVELAAEIVDHFKGKEVTIVHSQSKLMNRFPKKTIRYTEEFLQKRGVKLIYNERVVAHR
GQTFITDQGSEIIAEQAFLCTGIAPNSNFIKNSYPDAISENGYIKANDQLQMAGTTFYRN
IFVSGDVLHVREEKLAQTAECTASIVVNNINAMKVVVNIKCNLINLLQTCFNKSWKIFCY
LCL---

---*n*krkikkkkkmelkkkkkkkkkkkkkkkkkkkkkkkkk

Frame B:
lewswpqr*wtiskark*qscihnrn**tgsprrqyvtlrnsyksvvln*fimnvlwlig
vkhs*pirvvrsllskrsfvlvshqiqislktatpmpslrmvtsklttnsrwpalhsivi
slclvtsymyvkrs*pkllnaqlq*wsiismq*ksl*t*nail*tfckpvlislgkysai
fvy---

---ktkkek*kkkkkwn*kkkkkkkkkkkkkkkkkkkkkkkkk

Frame C:
wsgvgrrdsgpfqrqgsdnraftieineqvpqedntlh*giltkawc*idl**tccgs*g
snihnrsg**dhc*asvplywyrtkfkfh*kqlprchl*ewlhqs*rptpdgrhyils*y
lcvw*rltct*revspnc*mhsfnsgq*yqcnesrcehkmqsykpfanlf**vlenills
lfi---

---KLKKKNKKKKKNGIKKKKKKKKKKKKKKKKKKKKKKKKKK
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FC-BR21 (FC-BR21Q) /CSM/FC/FC-BR/FC-BR21Q.Seq.d/                     1092   0.0
SSD751 (SSD751Q) /CSM/SS/SSD7-C/SSD751Q.Seq.d/                       1065   0.0
SFH190 (SFH190Q) /CSM/SF/SFH1-D/SFH190Q.Seq.d/                       1065   0.0
SFE664 (SFE664Q) /CSM/SF/SFE6-C/SFE664Q.Seq.d/                       1065   0.0
CFI602 (CFI602Q) /CSM/CF/CFI6-A/CFI602Q.Seq.d/                       1065   0.0
AFE104 (AFE104Q) /CSM/AF/AFE1-A/AFE104Q.Seq.d/                       1065   0.0
AFD196 (AFD196Q) /CSM/AF/AFD1-D/AFD196Q.Seq.d/                       1065   0.0
AFA682 (AFA682Q) /CSM/AF/AFA6-D/AFA682Q.Seq.d/                       1065   0.0
SFI373 (SFI373Q) /CSM/SF/SFI3-D/SFI373Q.Seq.d/                       1059   0.0
SLA744 (SLA744Q) /CSM/SL/SLA7-B/SLA744Q.Seq.d/                       1053   0.0
own update 2001.11.26
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AU273878|AU273878.1 Hemicentrotus pulcherrimus mRNA, clone:001b02r_B08, 5' end, single read.    44  2.2  1
AC121441|AC121441.3 Rattus norvegicus clone CH230-20K16, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    34  3.4  4
AE010592|AE010592.1 Fusobacterium nucleatum subsp. nucleatum ATCC 25586, section 134 of 197 of the complete genome.    42  7.3  2
AX458634|AX458634.1 Sequence 180 from Patent WO0246454.           42  8.6  1
AX346461|AX346461.1 Sequence 1532 from Patent WO0200928.          42  8.6  1
CD508664|CD508664.1 CDA92-B03.x1d-t SHGC-CDA Gasterosteus aculeatus cDNA clone CDA92-B03 5', mRNA sequence.    42  8.6  1
CC267743|CC267743.1 CH261-184O17_Sp6.1 CH261 Gallus gallus genomic clone CH261-184O17, DNA sequence.    42  8.6  1
dna update 2003. 7.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q8BUE4) RecName: Full=Apoptosis-inducing factor 2;          EC=...    87   3e-16
BC038129_1(BC038129|pid:none) Mus musculus apoptosis-inducing fa...    87   3e-16
(Q9BRQ8) RecName: Full=Apoptosis-inducing factor 2;          EC=...    87   4e-16
AP007161_561(AP007161|pid:none) Aspergillus oryzae RIB40 genomic...    85   2e-15
BT021162_1(BT021162|pid:none) Bos taurus apoptosis-inducing fact...    84   4e-15
BX537621_1(BX537621|pid:none) Homo sapiens mRNA; cDNA DKFZp686H1...    83   8e-15
BC167890_1(BC167890|pid:none) Xenopus tropicalis cDNA clone MGC:...    78   3e-13
AJ749949_1425(AJ749949|pid:none) Francisella tularensis subsp. t...    75   1e-12
CP000439_1350(CP000439|pid:none) Francisella tularensis subsp. n...    74   3e-12
AK117798_1(AK117798|pid:none) Arabidopsis thaliana At5g22140 mRN...    74   3e-12
protein update 2009. 4.14
PSORT 

 psg: 0.75  gvh: 0.24  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 2.88  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for FC-BR21 is nuc
5' end seq. ID FC-BR21F
5' end seq. 
>FC-BR21F.Seq
GTTGGAGTGGAGTTGGCCGCAGAGATAGTGGACCATTTCAAAGGCAAGGAAGTGACAATC
GTGCATTCACAATCGAAATTAATGAACAGGTTCCCCAAGAAGACAATACGTTACACTGAG
GAATTCTTACAAAAGCGTGGTGTTAAATTGATTTATAATGAACGTGTTGTGGCTCATAGG
GGTCAAACATTCATAACCGATCAGGGTAGTGAGATCATTGCTGAGCAAGCGTTCCTTTGT
ACTGGTATCGCACCAAATTCAAATTTCATTAAAAACAGCTACCCCGATGCCATCTCTGAG
AATGGTTACATCAAAGCTAACGACCAACTCCAGATGGCCGGCACTACATTCTATCGTAAT
ATCTTTGTGTCTGGTGACGTCTTACATGTACGTGAAGAGAAGTTAGCCCAAACTGCTGAA
TGCACAGCTTCAATAGTGGTCAATAATATCAATGCAATGAAAGTCGTTGTGAACATAAAA
TGCAATCTTATAAACCTTTTGCAAACCTGTTTTAATAAGTCTTGGAAAATATTCTGCTAT
CTTTGTTTATA----------
Length of 5' end seq. 551
3' end seq. ID FC-BR21Z
3' end seq. 
>FC-BR21Z.Seq
----------TAAAACTAAAAAAGAAAAATAAAAAAAAAAAAAAAAATGGAATTAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAA
Length of 3' end seq. 122
Connected seq. ID FC-BR21P
Connected seq. 
>FC-BR21P.Seq
GTTGGAGTGGAGTTGGCCGCAGAGATAGTGGACCATTTCAAAGGCAAGGAAGTGACAATC
GTGCATTCACAATCGAAATTAATGAACAGGTTCCCCAAGAAGACAATACGTTACACTGAG
GAATTCTTACAAAAGCGTGGTGTTAAATTGATTTATAATGAACGTGTTGTGGCTCATAGG
GGTCAAACATTCATAACCGATCAGGGTAGTGAGATCATTGCTGAGCAAGCGTTCCTTTGT
ACTGGTATCGCACCAAATTCAAATTTCATTAAAAACAGCTACCCCGATGCCATCTCTGAG
AATGGTTACATCAAAGCTAACGACCAACTCCAGATGGCCGGCACTACATTCTATCGTAAT
ATCTTTGTGTCTGGTGACGTCTTACATGTACGTGAAGAGAAGTTAGCCCAAACTGCTGAA
TGCACAGCTTCAATAGTGGTCAATAATATCAATGCAATGAAAGTCGTTGTGAACATAAAA
TGCAATCTTATAAACCTTTTGCAAACCTGTTTTAATAAGTCTTGGAAAATATTCTGCTAT
CTTTGTTTATA----------TAAAACTAAAAAAGAAAAATAAAAAAAAAAAAAAAAATG
GAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAA
Length of connected seq. 673
Full length Seq ID -
Full length Seq. -
Length of full length seq. -