>AFI260 (AFI260Q) /CSM/AF/AFI2-C/AFI260Q.Seq.d/
XXXXXXXXXXCCAAGCAGGTAACCTCGCTGATGATTGGGAATTACTTCCATCAAAACAAA
TCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACC
CAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAG
CTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCG
CCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAG
GTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACC
TCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCT
TTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCT
TTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTG
AAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCG
AAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGAGACAA
AAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTT

---QAGNLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPEA
VKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNELYL
FNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAKAE
ERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTETKKKKSKNQPKK

Frame A:
---QAGNLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPEA
VKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNELYL
FNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAKAE
ERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTETKKKKSKNQPKK


Frame B:
---kqvtslmignyfhqnkskiqnnpnqsiglt*rksmtqkmlnqpvmtisqlqsliqkl
snqrigmkkttvngkpqpspiqntkvngkqrksqiqntkvngyth*litqntlktmnsts
lmisvlsvsnygklnlvpslti*ssptlskklkislkrpllptkkpkrkclttskppklk
rerkltkn*lpkkllkkkpkklmkkkkkslkki*lklrqkrrsqkinqks


Frame C:
---sr*pr**lgitsiktnqrsktiqtsrlg*rkgnr*prrc*tsrs*rypsfnr*srsc
qtrgle*rrrr*mgspnhrqsriqr*meskenpksriqr*mgtpin**prir*rq*tlpl
**srcyrfrimas*iwfhl*qydrhrlcrrs*rfl*kdlccqprsrkenv*rprsrqs*r
ekes*rkiscrksc*krsrrs**rrrrsr*rrfs*n*dkkeevkkstkkv

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM129 (VFM129Q) /CSM/VF/VFM1-B/VFM129Q.Seq.d/                       1059   0.0
VFL130 (VFL130Q) /CSM/VF/VFL1-B/VFL130Q.Seq.d/                       1059   0.0
VFI619 (VFI619Q) /CSM/VF/VFI6-A/VFI619Q.Seq.d/                       1059   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                       1059   0.0
VFE122 (VFE122Q) /CSM/VF/VFE1-A/VFE122Q.Seq.d/                       1059   0.0
SFK591 (SFK591Q) /CSM/SF/SFK5-D/SFK591Q.Seq.d/                       1059   0.0
SFH171 (SFH171Q) /CSM/SF/SFH1-C/SFH171Q.Seq.d/                       1059   0.0
SFG873 (SFG873Q) /CSM/SF/SFG8-D/SFG873Q.Seq.d/                       1059   0.0
CHC190 (CHC190Q) /CSM/CH/CHC1-D/CHC190Q.Seq.d/                       1059   0.0
CHC137 (CHC137Q) /CSM/CH/CHC1-B/CHC137Q.Seq.d/                       1059   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1059   0.0    1
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   1e-15  3
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   5e-11  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74   3e-09  1
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    74   3e-09  1
BE435540|BE435540.1 EST406618 tomato breaker fruit, TIGR Lycopersicon esculentum cDNA clone cLEG27F24, mRNA sequence.    74   3e-09  1
AI771812|AI771812.1 EST252912 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED38E1, mRNA sequence.    74   3e-09  1
AW033447|AW033447.1 EST277018 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28B21 similar to calreticulin, putative, mRNA sequence.    74   3e-09  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74   3e-09  1
AI782264|AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRNA sequence.    74   3e-09  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   323   2e-87
(Q9STD3) RecName: Full=Calreticulin; Flags: Precursor;  &AJ00076...   201   1e-50
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   199   5e-50
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   197   2e-49
AK230218_1(AK230218|pid:none) Arabidopsis thaliana mRNA for hypo...   196   5e-49
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   195   1e-48
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   195   1e-48
AC149080_5(AC149080|pid:none) Medicago truncatula clone mth2-75j...   193   3e-48
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   193   3e-48
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   192   6e-48

 psg: 0.75  gvh: 0.24  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.10  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for AFI260 is cyt

>AFI260Z.Seq
----------CCAAGCAGGTAACCTCGCTGATGATTGGGAATTACTTCCATCAAAACAAA
TCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACC
CAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAG
CTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCG
CCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAG
GTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACC
TCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCT
TTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCT
TTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTG
AAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCG
AAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGAGACAA
AAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTT