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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFG429
Library SF
(Link to library)
Clone ID SFG429
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16560-1
Original site URL
Representative seq. ID SFG429P
(Link to Original site)
Representative DNA sequence 
>SFG429 (SFG429Q) /CSM/SF/SFG4-B/SFG429Q.Seq.d/
CTGTTGGCCTACTGGATTTTTTCAAATTCATTTTTATTATACAAATATTAAATTCATATT
TTATTTTTTTATATATTGTAACATTTCATAAAAAAAAAAAAAAAAAAAAAATTTAAAAAN
TCNCNTTTTTTGAAAAAAAAAAXXXXXXXXXXTTGANAATGTAAANNNAANGATTNAANA
TAANNAGGTNTTNCCNNCANNNNNAACAANGTNTNANTTTTNGNTGGTAAANAATTAANA
ANATGGTNGNNCTNTTTNTNGANTTNCAAATATNCAAAAAANAATNAATTNTCCCACTTG
GTCNTCANNATTAANAAGGGTGGTANGCAAATNTTTGTCANAAACATTAACTGGTAAGAC
CATCNCTCTTGAAAGTTGAGGGCTCAGATAATATTGAAAATGTTAAAACAAAGATTCAAG
ACAAAGAAGGTATCCCACCAGACCAACAAAGATTAATTTTCGCTGGTAAACAATTAGAAG
ATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCCATTTAGTCCTCA
GATTAAGAGGTGGTATGCAAATTTTTGTCAAAACCCTCACTGGTAAGACTATTACACTCG
AAGTTGAAGGTTCTGATAACATTGAGAATGTTAAAGCCAAAATCCAAGACAAAGAAGGTA
TTCCACCAGATCAACAACGTCTCATTTTCGCCGGTAAACAATTGGAGGATGGTCGTACTC
TCTCTGATTACAACATTCAAAAGGAATCCACTCTCCATTTAGTTCTCAGATTAAGAGGTG
GTATGCAAATTTTTGTAAAAACTCTCACTGGTAAAACCATTACACTCGAAGTTGAAGGTA
GTGACAACATTGAGAATGTAAAAACAAAAATTCAAGACAAATAA
sequence update 2001.11.22
Translated Amino Acid sequence 
llaywifsnsfllyky*ihilffyil*HFIKKKKKKKFKXSXFLKKK---

---XNVXXXIXXXXVXPXXXTXXXFXXVXN*xxgxxxxxxxnxqkxxnxptwssxlxrvv
xkxlsxtltgktixles*glr*y*kc*nkdsrqrryptrptkinfrw*tirrwsysl*lq
hskgihspfspqikrwyanfcqnphw*dyytrs*rf**h*ec*sqnprqrrystrsttsh
frr*tiggwsysl*lqhskgihspfssqikrwyanfcknshw*nhytrs*r**qh*eckn
knsrqi
Translated Amino Acid sequence (All Frames) 
Frame A:
llaywifsnsfllyky*ihilffyil*HFIKKKKKKKFKXSXFLKKK---

---lxm*xxxxxixrxxxxxxqxxxfxw*xixxmvxlfxxxqixkkxxixplgxxx*xgw
xanxcxkh*lvrpsllkvegsdnienvktkiqdkegippdqqrlifagkqledgrtlsdy
niqkestlhlvlrlrggmqifvktltgktitlevegsdnienvkakiqdkegippdqqrl
ifagkqledgrtlsdyniqkestlhlvlrlrggmqifvktltgktitlevegsdnienvk
tkiqdk*

Frame B:
cwptgffqihfyytnikfifyffiycnis*kkkkkknlkxxxf*kkk---

---*xckxxdxx*xgxxxxxnxvxxxxgkxlxxwxxxxxxxkyxkxnxxshlvxxixkgg
xqxfvxninw*dhxs*klraqiilkmlkqrfktkkvshqtnkd*fslvnn*kmvvlslit
tfkrnplsi*ssd*evvckflskpslvrllhsklkvlitlrmlkpksktkkvfhqinnvs
fspvnnwrmvvlslittfkrnplsi*fsd*evvckfl*klslvkplhsklkvvttlrm*k
qkfktn

Frame C:
vglldffkfifiiqilnsyfiflyivtfhkkkkkkki*kxxffekk---

---XNVXXXIXXXXVXPXXXTXXXFXXVXN*xxgxxxxxxxnxqkxxnxptwssxlxrvv
xkxlsxtltgktixles*glr*y*kc*nkdsrqrryptrptkinfrw*tirrwsysl*lq
hskgihspfspqikrwyanfcqnphw*dyytrs*rf**h*ec*sqnprqrrystrsttsh
frr*tiggwsysl*lqhskgihspfssqikrwyanfcknshw*nhytrs*r**qh*eckn
knsrqi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFG429 (SFG429Q) /CSM/SF/SFG4-B/SFG429Q.Seq.d/                       1176   0.0
SFL419 (SFL419Q) /CSM/SF/SFL4-A/SFL419Q.Seq.d/                       1080   0.0
VFI713 (VFI713Q) /CSM/VF/VFI7-A/VFI713Q.Seq.d/                       1078   0.0
CFI465 (CFI465Q) /CSM/CF/CFI4-C/CFI465Q.Seq.d/                       1078   0.0
SFJ202 (SFJ202Q) /CSM/SF/SFJ2-A/SFJ202Q.Seq.d/                       1076   0.0
SFI270 (SFI270Q) /CSM/SF/SFI2-C/SFI270Q.Seq.d/                       1076   0.0
CFF113 (CFF113Q) /CSM/CF/CFF1-A/CFF113Q.Seq.d/                       1076   0.0
AFL334 (AFL334Q) /CSM/AF/AFL3-B/AFL334Q.Seq.d/                       1076   0.0
AFD894 (AFD894Q) /CSM/AF/AFD8-D/AFD894Q.Seq.d/                       1076   0.0
AFB143 (AFB143Q) /CSM/AF/AFB1-B/AFB143Q.Seq.d/                       1076   0.0
own update 2003. 5.29
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M19491|M19491.1 Slime mold (D.discoideum) ubiquitin p229C/pLK229 gene, complete cds.  1001   0.0    2
M23748|M23748.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB19.   993   0.0    3
M19666|M19666.1 Slime mold (D.discoideum) ubiquitin gene, 3' end, clone p229C.   993   0.0    2
M23754|M23754.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB2.   597   e-174  2
M23753|M23753.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB14.   617   e-172  2
M19492|M19492.1 Slime mold (D.discoideum) ubiquitin lambda-229 gene, complete cds.   585   e-170  3
M23752|M23752.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB17.   472   e-129  2
M23751|M23751.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB18.   472   e-129  2
BJ528884|BJ528884.1 Oryzias latipes cDNA clone:MF01SSB034A18, 5' end single read.   192   2e-84  4
CF181901|CF181901.1 ISO3B1F Iso cDNA Isotricha sp. BBF-2003 cDNA similar to PfpUB polyubiquitin 3D7, mRNA sequence.    96   3e-79  5
dna update 2003.12.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27806(A27806) polyubiquitin 5 (clone pLK229) - slime mold (Dict...   333   6e-90
M19666_1(M19666|pid:none) Slime mold (D.discoideum) ubiquitin ge...   333   6e-90
B34080(B34080)polyubiquitin 5 (clone DCUB19) - slime mold (Dicty...   333   6e-90
B27806(B27806) ubiquitin (clone lambda229) - slime mold (Dictyos...   329   8e-89
M23754_1(M23754|pid:none) D.discoideum ubiquitin mRNA, complete ...   329   8e-89
S53719(S53719;S73107;S71069)polyubiquitin 6 - red alga (Gracilar...   317   2e-85
AF517848_1(AF517848|pid:none) Griffithsia japonica isolate Gj526...   317   2e-85
AY957885_1(AY957885|pid:none) Gracilaria lemaneiformis polyubiqu...   317   2e-85
AB021974_1(AB021974|pid:none) Bombyx mori UBI3 mRNA for polyubiq...   315   1e-84
Y12081_1(Y12081|pid:none) Suberites domuncula mRNA for polyubiqu...   315   1e-84
protein update 2009. 5.24
PSORT 

 psg: 0.68  gvh: 0.49  alm: 0.56  top: 0.53  tms: 0.00  mit: 0.39  mip: 0.00
 nuc: 0.47  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 76.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: cytoplasmic

>> prediction for SFG429 is nuc
5' end seq. ID SFG429F
5' end seq. 
>SFG429F.Seq
CTGTTGGCCTACTGGATTTTTTCAAATTCATTTTTATTATACAAATATTAAATTCATATT
TTATTTTTTTATATATTGTAACATTTCATAAAAAAAAAAAAAAAAAAAAAATTTAAAAAN
TCNCNTTTTTTGAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 152
3' end seq. ID SFG429Z
3' end seq. 
>SFG429Z.Seq
----------TTGANAATGTAAANNNAANGATTNAANATAANNAGGTNTTNCCNNCANNN
NNAACAANGTNTNANTTTTNGNTGGTAAANAATTAANAANATGGTNGNNCTNTTTNTNGA
NTTNCAAATATNCAAAAAANAATNAATTNTCCCACTTGGTCNTCANNATTAANAAGGGTG
GTANGCAAATNTTTGTCANAAACATTAACTGGTAAGACCATCNCTCTTGAAAGTTGAGGG
CTCAGATAATATTGAAAATGTTAAAACAAAGATTCAAGACAAAGAAGGTATCCCACCAGA
CCAACAAAGATTAATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTA
CAACATTCAAAAGGAATCCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAAT
TTTTGTCAAAACCCTCACTGGTAAGACTATTACACTCGAAGTTGAAGGTTCTGATAACAT
TGAGAATGTTAAAGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCT
CATTTTCGCCGGTAAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAA
GGAATCCACTCTCCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAAC
TCTCACTGGTAAAACCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAA
AACAAAAATTCAAGACAAATAA
Length of 3' end seq. 732
Connected seq. ID SFG429P
Connected seq. 
>SFG429P.Seq
CTGTTGGCCTACTGGATTTTTTCAAATTCATTTTTATTATACAAATATTAAATTCATATT
TTATTTTTTTATATATTGTAACATTTCATAAAAAAAAAAAAAAAAAAAAAATTTAAAAAN
TCNCNTTTTTTGAAAAAAAAAA----------TTGANAATGTAAANNNAANGATTNAANA
TAANNAGGTNTTNCCNNCANNNNNAACAANGTNTNANTTTTNGNTGGTAAANAATTAANA
ANATGGTNGNNCTNTTTNTNGANTTNCAAATATNCAAAAAANAATNAATTNTCCCACTTG
GTCNTCANNATTAANAAGGGTGGTANGCAAATNTTTGTCANAAACATTAACTGGTAAGAC
CATCNCTCTTGAAAGTTGAGGGCTCAGATAATATTGAAAATGTTAAAACAAAGATTCAAG
ACAAAGAAGGTATCCCACCAGACCAACAAAGATTAATTTTCGCTGGTAAACAATTAGAAG
ATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCCATTTAGTCCTCA
GATTAAGAGGTGGTATGCAAATTTTTGTCAAAACCCTCACTGGTAAGACTATTACACTCG
AAGTTGAAGGTTCTGATAACATTGAGAATGTTAAAGCCAAAATCCAAGACAAAGAAGGTA
TTCCACCAGATCAACAACGTCTCATTTTCGCCGGTAAACAATTGGAGGATGGTCGTACTC
TCTCTGATTACAACATTCAAAAGGAATCCACTCTCCATTTAGTTCTCAGATTAAGAGGTG
GTATGCAAATTTTTGTAAAAACTCTCACTGGTAAAACCATTACACTCGAAGTTGAAGGTA
GTGACAACATTGAGAATGTAAAAACAAAAATTCAAGACAAATAA
Length of connected seq. 874
Full length Seq ID -
Full length Seq. -
Length of full length seq. -