>SFL375 (SFL375Q) /CSM/SF/SFL3-D/SFL375Q.Seq.d/
AGAGTTGTTATTTTTTTTTTATTTAAAACTTTTTTCCCACACCAATATTGAAATAAAAAA
TGAATAATCAACAAGCAACATATACCAGAAGAATTCAAAATATTCAATCACATATTGTAA
ATAGTAATAATAATAGTAATAATAGTAATAATAATAATAATATTAATTTTAATAATAAAA
ATAATAATAATAATGAAGTATTATATGATTGTATTGTAATTGGNGGNGGTATCACAGGAT
CATCAGCATGTTATCAAATGGCAAAAGATGGATTAAAGGTATTAATGTTAGAACAATTTA
AAGAAGCACATGATAAAGGATCATCACATGGCGATGGTAGAATTATAAGATTTTCATATC
CAGAGGATACCTATATTAGATTAGCTAAATTGGTTTATCCAGAATGGTCAGAGATTGAAA
GATTATCAAATNCCAAATTAATTCATATCXXXXXXXXXXGTCACTCATANCTGTATTATG
TGAAGATCAATCAGTTTATAAAACTAAAAAAATAGTTTTAGCATGTGGTGGTTGGATTAA
TGATTTAATTCATAATTCACAATTAAACATTACATTACCTTTAGAAATCTCTCAAGAAAC
TGTATTTTACTGGGAACCAAAATTAAATAATACAATTGATTATACTCAATTTAATAACCC
TGTTTCAATCTTTTATGATAATAATTCTGATGTTTATTATTCTTTACCTCAAATTGATGT
TAAAGGGGTTAAAATTGGATATCATCATAGTGGTAATGTTTTGGAAAATATGGAAAGTAA
AAAATTACCATTTAAAAAAGAAAATTATGATAAAGTTAAAGGATATGTTGGTGAATATAT
GCCAGGTTTAAATAATGATAAAGAGGGTCATAGCGTTGTTTGCCATTACACAAATACATC
AGATTGGCATTTTATTATTGATAAACATCCAAGATTTACAAATGTTGTTATTGCATCACC
TTGTTCCGGTCATGGTTTTAAGTTTGGTCCTGCCATGGAAAATTAATTTCAAATTAGTTC
AAAATAAACCAAATCCAATNATACAAANAGAATTTTTATTAAAAAGATTCAATTCCAAAA
CTTTCCCAAAAAG

scyfffi*nffptpilk*KMNNQQATYTRRIQNIQSHIVNSNNNSNNSNNNNNINFNNKN
NNNNEVLYDCIVIGGGITGSSACYQMAKDGLKVLMLEQFKEAHDKGSSHGDGRIIRFSYP
EDTYIRLAKLVYPEWSEIERLSNXKLIHI---

---SLIXVLCEDQSVYKTKKIVLACGGWINDLIHNSQLNITLPLEISQETVFYWEPKLNN
TIDYTQFNNPVSIFYDNNSDVYYSLPQIDVKGVKIGYHHSGNVLENMESKKLPFKKENYD
KVKGYVGEYMPGLNNDKEGHSVVCHYTNTSDWHFIIDKHPRFTNVVIASPCSGHGFKFGP
AMEN*fqissk*tksnxtxrifikkiqfqnfpkk

Frame A:
rvvifflfktffphqy*nkk*iinkqhipeefkifnhil*iviiiviiviiiiililiik
iiiimkyymivl*lxxvsqdhqhvikwqkmd*ry*c*nnlkkhmikdhhmamvel*dfhi
qripild*lnwfiqngqrlkdyqxpn*fi---

---vthxcim*rsisl*n*knsfsmwwld**fns*ftikhyitfrnlsrncillgtkik*
yn*lysi**pcfnll***f*cllfftsn*c*rg*nwiss*w*cfgkygk*kiti*krkl*
*s*ricw*iyarfk***rgs*rclplhkyirlafyy**tskiykccycitlfrswf*vws
chgklisn*fkinqiqxykxnfy*kdsipklsqk

Frame B:
ellfffylklfshtnieikne*stsniyqknskysityck*************y*f***k
*****sii*lycnwxxyhriismlsngkrwikginvrti*rst**riitwrw*nykifis
rgyly*is*iglsrmvrd*kiikxqinsy---

---SLIXVLCEDQSVYKTKKIVLACGGWINDLIHNSQLNITLPLEISQETVFYWEPKLNN
TIDYTQFNNPVSIFYDNNSDVYYSLPQIDVKGVKIGYHHSGNVLENMESKKLPFKKENYD
KVKGYVGEYMPGLNNDKEGHSVVCHYTNTSDWHFIIDKHPRFTNVVIASPCSGHGFKFGP
AMEN*fqissk*tksnxtxrifikkiqfqnfpkk

Frame C:
scyfffi*nffptpilk*KMNNQQATYTRRIQNIQSHIVNSNNNSNNSNNNNNINFNNKN
NNNNEVLYDCIVIGGGITGSSACYQMAKDGLKVLMLEQFKEAHDKGSSHGDGRIIRFSYP
EDTYIRLAKLVYPEWSEIERLSNXKLIHI---

---hsxlyyvkinqfiklkk*f*hvvvglmi*fiihn*tlhyl*kslkklyftgnqn*ii
qliilnlitlfqsfmiiilmfiilylklmlkglkldiiivvmfwkiwkvknyhlkkkimi
klkdmlvnicqv*imikrvialfaitqihqigillliniqdlqmlllhhlvpvmvlslvl
pwkinfklvqnkpnpxiqxefllkrfnsktfpk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFL375 (SFL375Q) /CSM/SF/SFL3-D/SFL375Q.Seq.d/                       1021   0.0
SSE158 (SSE158Q) /CSM/SS/SSE1-C/SSE158Q.Seq.d/                         42   0.018
SLC138 (SLC138Q) /CSM/SL/SLC1-B/SLC138Q.Seq.d/                         42   0.018
CFC104 (CFC104Q) /CSM/CF/CFC1-A/CFC104Q.Seq.d/                         42   0.018
SSE249 (SSE249Q) /CSM/SS/SSE2-C/SSE249Q.Seq.d/                         40   0.070
VSD656 (VSD656Q) /CSM/VS/VSD6-C/VSD656Q.Seq.d/                         38   0.28
SSL392 (SSL392Q) /CSM/SS/SSL3-D/SSL392Q.Seq.d/                         38   0.28
SSF573 (SSF573Q) /CSM/SS/SSF5-D/SSF573Q.Seq.d/                         38   0.28
SSD310 (SSD310Q) /CSM/SS/SSD3-A/SSD310Q.Seq.d/                         38   0.28
SFH639 (SFH639Q) /CSM/SF/SFH6-B/SFH639Q.Seq.d/                         38   0.28

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC114263|AC114263.2 Dictyostelium discoideum chromosome 2 map 215673-367476 strain AX4, complete sequence.    36   1e-06  16
AE014821|AE014821.1 Plasmodium falciparum 3D7 chromosome 14 section 6 of 13 of the complete sequence.    34   5e-06  16
AL035476|AL035476.7 Plasmodium falciparum MAL4P3.                  42   1e-05  15
AE014842|AE014842.1 Plasmodium falciparum 3D7 chromosome 11 section 7 of 8 of the complete sequence.    36   4e-05  14
AB063521|AB063521.2 Wigglesworthia brevipalpis DNA, complete genome, section 1/2.    36   6e-05  16
AL049184|AL049184.5 Plasmodium falciparum DNA *** SEQUENCING IN PROGRESS *** from  MAL13P3.    40   7e-05  16
AP006628|AP006628.1 Onion yellows phytoplasma DNA, complete genome.    32   1e-04  26
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    30   2e-04  20
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    34   3e-04  19
AE014846|AE014846.1 Plasmodium falciparum 3D7 chromosome 12, section 3 of 9 of the complete sequence.    40   4e-04  13

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P40859) RecName: Full=Monomeric sarcosine oxidase;          Sho...   102   2e-20
AY626822_1(AY626822|pid:none) Bacillus sp. BSD-8 monomeric sarco...   102   3e-20
CP000431_3269(CP000431|pid:none) Rhodococcus jostii RHA1, comple...    92   2e-17
(Q9D826) RecName: Full=Peroxisomal sarcosine oxidase;          S...    92   4e-17
AP011115_2847(AP011115|pid:none) Rhodococcus opacus B4 DNA, comp...    88   4e-16
BC088249_1(BC088249|pid:none) Rattus norvegicus pipecolic acid o...    88   6e-16
(Q29RU9) RecName: Full=Peroxisomal sarcosine oxidase;          S...    87   1e-15
BC114006_1(BC114006|pid:none) Bos taurus L-pipecolic acid oxidas...    87   1e-15
BC116493_1(BC116493|pid:none) Danio rerio similar to Peroxisomal...    86   2e-15
BC124587_1(BC124587|pid:none) Danio rerio similar to Peroxisomal...    86   2e-15

 psg: 0.53  gvh: 0.43  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.43  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: mitochondrial
	 32.0 %: nuclear
	 12.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for SFL375 is mit

>SFL375F.Seq
AGAGTTGTTATTTTTTTTTTATTTAAAACTTTTTTCCCACACCAATATTGAAATAAAAAA
TGAATAATCAACAAGCAACATATACCAGAAGAATTCAAAATATTCAATCACATATTGTAA
ATAGTAATAATAATAGTAATAATAGTAATAATAATAATAATATTAATTTTAATAATAAAA
ATAATAATAATAATGAAGTATTATATGATTGTATTGTAATTGGNGGNGGTATCACAGGAT
CATCAGCATGTTATCAAATGGCAAAAGATGGATTAAAGGTATTAATGTTAGAACAATTTA
AAGAAGCACATGATAAAGGATCATCACATGGCGATGGTAGAATTATAAGATTTTCATATC
CAGAGGATACCTATATTAGATTAGCTAAATTGGTTTATCCAGAATGGTCAGAGATTGAAA
GATTATCAAATNCCAAATTAATTCATATC----------

>SFL375Z.Seq
----------GTCACTCATANCTGTATTATGTGAAGATCAATCAGTTTATAAAACTAAAA
AAATAGTTTTAGCATGTGGTGGTTGGATTAATGATTTAATTCATAATTCACAATTAAACA
TTACATTACCTTTAGAAATCTCTCAAGAAACTGTATTTTACTGGGAACCAAAATTAAATA
ATACAATTGATTATACTCAATTTAATAACCCTGTTTCAATCTTTTATGATAATAATTCTG
ATGTTTATTATTCTTTACCTCAAATTGATGTTAAAGGGGTTAAAATTGGATATCATCATA
GTGGTAATGTTTTGGAAAATATGGAAAGTAAAAAATTACCATTTAAAAAAGAAAATTATG
ATAAAGTTAAAGGATATGTTGGTGAATATATGCCAGGTTTAAATAATGATAAAGAGGGTC
ATAGCGTTGTTTGCCATTACACAAATACATCAGATTGGCATTTTATTATTGATAAACATC
CAAGATTTACAAATGTTGTTATTGCATCACCTTGTTCCGGTCATGGTTTTAAGTTTGGTC
CTGCCATGGAAAATTAATTTCAAATTAGTTCAAAATAAACCAAATCCAATNATACAAANA
GAATTTTTATTAAAAAGATTCAATTCCAAAACTTTCCCAAAAAG

>SFL375P.Seq
AGAGTTGTTATTTTTTTTTTATTTAAAACTTTTTTCCCACACCAATATTGAAATAAAAAA
TGAATAATCAACAAGCAACATATACCAGAAGAATTCAAAATATTCAATCACATATTGTAA
ATAGTAATAATAATAGTAATAATAGTAATAATAATAATAATATTAATTTTAATAATAAAA
ATAATAATAATAATGAAGTATTATATGATTGTATTGTAATTGGNGGNGGTATCACAGGAT
CATCAGCATGTTATCAAATGGCAAAAGATGGATTAAAGGTATTAATGTTAGAACAATTTA
AAGAAGCACATGATAAAGGATCATCACATGGCGATGGTAGAATTATAAGATTTTCATATC
CAGAGGATACCTATATTAGATTAGCTAAATTGGTTTATCCAGAATGGTCAGAGATTGAAA
GATTATCAAATNCCAAATTAATTCATATC----------GTCACTCATANCTGTATTATG
TGAAGATCAATCAGTTTATAAAACTAAAAAAATAGTTTTAGCATGTGGTGGTTGGATTAA
TGATTTAATTCATAATTCACAATTAAACATTACATTACCTTTAGAAATCTCTCAAGAAAC
TGTATTTTACTGGGAACCAAAATTAAATAATACAATTGATTATACTCAATTTAATAACCC
TGTTTCAATCTTTTATGATAATAATTCTGATGTTTATTATTCTTTACCTCAAATTGATGT
TAAAGGGGTTAAAATTGGATATCATCATAGTGGTAATGTTTTGGAAAATATGGAAAGTAA
AAAATTACCATTTAAAAAAGAAAATTATGATAAAGTTAAAGGATATGTTGGTGAATATAT
GCCAGGTTTAAATAATGATAAAGAGGGTCATAGCGTTGTTTGCCATTACACAAATACATC
AGATTGGCATTTTATTATTGATAAACATCCAAGATTTACAAATGTTGTTATTGCATCACC
TTGTTCCGGTCATGGTTTTAAGTTTGGTCCTGCCATGGAAAATTAATTTCAAATTAGTTC
AAAATAAACCAAATCCAATNATACAAANAGAATTTTTATTAAAAAGATTCAATTCCAAAA
CTTTCCCAAAAAG