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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHA257
Library SH
(Link to library)
Clone ID SHA257
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11926-1
Original site URL
Representative seq. ID SHA257P
(Link to Original site)
Representative DNA sequence 
>SHA257 (SHA257Q) /CSM/SH/SHA2-C/SHA257Q.Seq.d/
ATGTACCGTAGACTCATGTTCAAATTCAACCGGTTGTGTAAACACTCCAGTCAATGTTGA
TGATAATAATCCATGTACTGTAGATGCTTGTACCAAATCAACAGGTGTCACACATACCCC
AGTAAATGTAGATGATAACAACAAATGTACAATTGATGCATGTACCAAAGAAGGTGGTGT
AACTCATACTCCAGTCAACACTGATGATAACAATGCCTGTACAATTGATTCTTGTTCACC
ATCAACNGGTATTTCCCACACCCCAATCAATTGTGATGATAAAAANGCCTGTACAGTTGA
TTCATGTTCAAATTCAACTGGTTGTGTAAATACTCCAATTNNCTGTGACGATAATAATCC
ATGTACTGTNGATTCTTGCNATGACTTAACTGGTNGTTGNNATACTCCAATCAATGTTGA
TGATAATAANNCATGTACCATCGATGCCTGTACTAAATCAACAGGTGNTACTCATACCCC
AGTCAATGNNGATGATAATAACAAATGTACAATTGATACNTGTACCAAAGAAGGTGGTGT
AACTCATACTCCACTCAATACTGATGATAACAATGCCTGTACCCTTGATTCCTGCTCACC
ATCAACTGGTGTNTCCCATACCCCAATNNACTGTGATGATAATAATANATGNACTGTANA
TTCATGTTNAAACTCAACTGGGGGNCCGAATACTCCAATTAACTGGGGACGAAAGNAANC
CCTGTNCCGGAAACTCATGCCATNAANCACNGGGGTGGGTAACNCTCNCTCAATGGTGGT
GANAAAANNCATGNCCCGTCNATNCCTGXXXXXXXXXXACTCATACACCAATCAATACAG
ATGATAATACCAAATGTACATTAGATGCTTGTTCACCAAAGACTGGTGTAACTCATACAC
CAATCAATTGTGATGATGGAAACAAATGTACAATCAATAGTTGTTCACCATCAGTTGGTT
GTATCTCAACACCAGTTTCATGTCCAAAACCAAAAGATAAATGTTCAATCTCTCAATGTG
ATTCAGCCAAAGGTTGCATCGAAGTCCCAATGAATTGTACCTCTGATAAATGTAATGAAG
CATCATGTTGTGATGGTGTTTGTACCTCAAAACCAATTAGCTGTCCAAAACCAAAGAATA
AGTGTCAAGTTGCAAAATGTGATTTAATTAAAGGTTGTACCGTCTCAAATGTAGTATGTG
ATGATGGTAATGCTTGTACCGAAGATTCATGTTGTTCAGACACTGGTAAATGTCAATTCG
AACCAATCAAACTTCCAAAGAATAAAAACAAATGTATCATTTCAAAATGTGATCCAATTA
AAGGTACAATCACCAACAGTACCGTAAACTGTGAATGTGATGACCTTTGTAACATTGGTG
AATGTTGTGAAGATACAGGAAAATGTAATTACAGACAAAAAGATTGTGATGATAATAATC
CAAAAACAGCTGATAGTTGCGATTCCAAGACTGGTAAATGTATTAACAAACCATATAATG
TTATCACAAGTGGTTCTAATTTAATCTCTGGTTTAATTGGTGGTCTCATTGGTGGTGGTA
CAGGAGATAAAGGNATTGCAAAACTGTAAAAATTAAANA
sequence update 2002.10.25
Translated Amino Acid sequence 
CTVDSCSNSTGCVNTPVNVDDNNPCTVDACTKSTGVTHTPVNVDDNNKCTIDACTKEGGV
THTPVNTDDNNACTIDSCSPSTGISHTPINCDDKXACTVDSCSNSTGCVNTPIXCDDNNP
CTVDSCXDLTGXXXTPINVDDNXXCTIDACTKSTGXTHTPVNXDDNNKCTIDTCTKEGGV
THTPLNTDDNNACTLDSCSPSTGVSHTPXXCDDNNXXTVXSCXNSTGGPNTPINWGRKXX
LXRKLMPXXTGVGNXXSMVVXKXMXRXXL---

---THTPINTDDNTKCTLDACSPKTGVTHTPINCDDGNKCTINSCSPSVGCISTPVSCPK
PKDKCSISQCDSAKGCIEVPMNCTSDKCNEASCCDGVCTSKPISCPKPKNKCQVAKCDLI
KGCTVSNVVCDDGNACTEDSCCSDTGKCQFEPIKLPKNKNKCIISKCDPIKGTITNSTVN
CECDDLCNIGECCEDTGKCNYRQKDCDDNNPKTADSCDSKTGKCINKPYNVITSGSNLIS
GLIGGLIGGGTGDKGIAKL*klx
Translated Amino Acid sequence (All Frames) 
Frame A:
myrrlmfkfnrlckhssqc****smycrclyqinrchtypskcr**qqmyn*cmyqrrwc
nsyssqh***qclyn*flftinxyfphpnql***kxlys*fmfkfnwlckysnxl*r**s
mycxflx*lnwxlxysnqc****xmyhrcly*inrxysypsqxx***qmyn*yxyqrrwc
nsystqy***qclyp*flltinwcxpypnxl****xmxcxfmxklnwgxeysn*lgtkxx
pvpethaxxhxgg*xslnggxkxhxpsxp---

---THTPINTDDNTKCTLDACSPKTGVTHTPINCDDGNKCTINSCSPSVGCISTPVSCPK
PKDKCSISQCDSAKGCIEVPMNCTSDKCNEASCCDGVCTSKPISCPKPKNKCQVAKCDLI
KGCTVSNVVCDDGNACTEDSCCSDTGKCQFEPIKLPKNKNKCIISKCDPIKGTITNSTVN
CECDDLCNIGECCEDTGKCNYRQKDCDDNNPKTADSCDSKTGKCINKPYNVITSGSNLIS
GLIGGLIGGGTGDKGIAKL*klx

Frame B:
CTVDSCSNSTGCVNTPVNVDDNNPCTVDACTKSTGVTHTPVNVDDNNKCTIDACTKEGGV
THTPVNTDDNNACTIDSCSPSTGISHTPINCDDKXACTVDSCSNSTGCVNTPIXCDDNNP
CTVDSCXDLTGXXXTPINVDDNXXCTIDACTKSTGXTHTPVNXDDNNKCTIDTCTKEGGV
THTPLNTDDNNACTLDSCSPSTGVSHTPXXCDDNNXXTVXSCXNSTGGPNTPINWGRKXX
LXRKLMPXXTGVGNXXSMVVXKXMXRXXL---

---lihqsiqmiipnvh*mlvhqrlv*lihqsivmmetnvqsivvhhqlvvsqhqfhvqn
qkinvqslnviqpkvasksq*ivplinvmkhhvvmvfvpqnqlavqnqrisvklqnvi*l
kvvpsqm*yvmmvmlvpkihvvqtlvnvnsnqsnfqriktnvsfqnviqlkvqsptvp*t
vnvmtfvtlvnvvkiqenvitdkkivmiiiqkqlivaiprlvnvltnhimlsqvvli*sl
v*lvvslvvvqeikxlqnckn*x

Frame C:
vp*thvqiqpvv*tlqsmlmiiihvl*mlvpnqqvshipq*m*mittnvqlmhvpkkvv*
lilqstlmitmpvqlilvhhqxvfptpqsivmikxpvqlihvqiqlvv*ilqxxvtiiih
vlxilamt*lvvxilqsmlmiixhvpsmpvlnqqvxlipqsmxmiitnvqlixvpkkvv*
lilhsilmitmpvplipahhqlvxpipqxtvmiiixxlxihvxtqlgxrilqltgdexxp
cxgnschxxxgwvtlxqww*xxxxpvxx---

---sytnqyr**yqmyirclftkdwcnsytnql**wkqmynq*lftiswlylntsfmskt
kr*mfnlsm*fsqrlhrspnelyl**m**siml*wclylktn*lsktke*vssckm*fn*
rlyrlkcsm**w*clyrrfmlfrhw*msirtnqtske*kqmyhfkm*sn*rynhqqyrkl
*m**pl*hw*ml*ryrkm*lqtkrl****skns**lrfqdw*my*qti*cyhkwf*fnlw
fnwwshwwwyrr*rxcktvkik
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHA257 (SHA257Q) /CSM/SH/SHA2-C/SHA257Q.Seq.d/                       2880   0.0
SHA152 (SHA152Q) /CSM/SH/SHA1-C/SHA152Q.Seq.d/                       1522   0.0
CFC807 (CFC807Q) /CSM/CF/CFC8-A/CFC807Q.Seq.d/                       1520   0.0
SHA221 (SHA221Q) /CSM/SH/SHA2-A/SHA221Q.Seq.d/                       1509   0.0
CFC542 (CFC542Q) /CSM/CF/CFC5-B/CFC542Q.Seq.d/                       1509   0.0
SHC394 (SHC394Q) /CSM/SH/SHC3-D/SHC394Q.Seq.d/                       1503   0.0
CHS294 (CHS294Q) /CSM/CH/CHS2-D/CHS294Q.Seq.d/                       1501   0.0
CHM421 (CHM421Q) /CSM/CH/CHM4-A/CHM421Q.Seq.d/                       1501   0.0
SHA109 (SHA109Q) /CSM/SH/SHA1-A/SHA109Q.Seq.d/                       1493   0.0
SHG420 (SHG420Q) /CSM/SH/SHG4-A/SHG420Q.Seq.d/                       1487   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X78948|X78948.1 D.minutum ecmB gene.                               88   2e-34  6
AF134686|AF134686.1 Plasmodium falciparum strain IVC10 from Cote d' Ivoire CG2 omega repeat (cg2) gene, partial cds.    32   3e-04  5
AF134662|AF134662.1 Plasmodium falciparum strain UPV4 from Burkina Faso CG2 omega repeat (cg2) gene, partial cds.    32   0.018  4
AF134664|AF134664.1 Plasmodium falciparum strain IVC17 from Cote d' Ivoire CG2 omega repeat (cg2) gene, partial cds.    32   0.022  4
AF134658|AF134658.1 Plasmodium falciparum strain SEN16 from Senegal CG2 omega repeat (cg2) gene, partial cds.    32   0.025  4
AF134671|AF134671.1 Plasmodium falciparum strain TOG1 from Togo CG2 omega repeat (cg2) gene, partial cds.    32   0.028  4
AF134670|AF134670.1 Plasmodium falciparum strain SEN10 from Senegal CG2 omega repeat (cg2) gene, partial cds.    32   0.028  4
AF134672|AF134672.1 Plasmodium falciparum strain HAI1 from Haiti CG2 omega repeat (cg2) gene, partial cds.    32   0.030  4
AF134660|AF134660.1 Plasmodium falciparum strain COM6 from Comoros CG2 omega repeat (cg2) gene, partial cds.    32   0.032  4
AF134676|AF134676.1 Plasmodium falciparum strain CAE6 from Cameroon CG2 omega repeat (cg2) gene, partial cds.    32   0.032  4
dna update 2005.12. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27020(A27020)DIF-induced prestalk pDd63 protein precursor - sli...   422   0.0
(P11976) RecName: Full=Prestalk protein; AltName: Full=Extracell...   332   e-139
A26838(A26838)prestalk protein precursor - slime mold (Dictyoste...   331   e-139
S44208(S44208) extracellular matrix protein B - Dictyostelium mi...   286   1e-75
AC117072_61(AC117072|pid:none) Dictyostelium discoideum chromoso...   249   1e-73
AC116984_103(AC116984|pid:none) Dictyostelium discoideum chromos...   253   4e-72
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                131   7e-29
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                125   3e-27
(Q54C31) RecName: Full=Protein psiR; Flags: Precursor;                106   2e-21
(Q94494) RecName: Full=Protein psiH; Flags: Precursor;  &U67940_...   101   8e-20
protein update 2009. 4.14
PSORT 

 psg: 0.75  gvh: 0.33  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.60  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 20.0 %: mitochondrial
	 20.0 %: peroxisomal
	 12.0 %: nuclear
	  4.0 %: cytoskeletal

>> prediction for SHA257 is cyt
5' end seq. ID SHA257F
5' end seq. 
>SHA257F.Seq
ATGTACCGTAGACTCATGTTCAAATTCAACCGGTTGTGTAAACACTCCAGTCAATGTTGA
TGATAATAATCCATGTACTGTAGATGCTTGTACCAAATCAACAGGTGTCACACATACCCC
AGTAAATGTAGATGATAACAACAAATGTACAATTGATGCATGTACCAAAGAAGGTGGTGT
AACTCATACTCCAGTCAACACTGATGATAACAATGCCTGTACAATTGATTCTTGTTCACC
ATCAACNGGTATTTCCCACACCCCAATCAATTGTGATGATAAAAANGCCTGTACAGTTGA
TTCATGTTCAAATTCAACTGGTTGTGTAAATACTCCAATTNNCTGTGACGATAATAATCC
ATGTACTGTNGATTCTTGCNATGACTTAACTGGTNGTTGNNATACTCCAATCAATGTTGA
TGATAATAANNCATGTACCATCGATGCCTGTACTAAATCAACAGGTGNTACTCATACCCC
AGTCAATGNNGATGATAATAACAAATGTACAATTGATACNTGTACCAAAGAAGGTGGTGT
AACTCATACTCCACTCAATACTGATGATAACAATGCCTGTACCCTTGATTCCTGCTCACC
ATCAACTGGTGTNTCCCATACCCCAATNNACTGTGATGATAATAATANATGNACTGTANA
TTCATGTTNAAACTCAACTGGGGGNCCGAATACTCCAATTAACTGGGGACGAAAGNAANC
CCTGTNCCGGAAACTCATGCCATNAANCACNGGGGTGGGTAACNCTCNCTCAATGGTGGT
GANAAAANNCATGNCCCGTCNATNCCTGNNNNNNNNNN
Length of 5' end seq. 818
3' end seq. ID SHA257Z
3' end seq. 
>SHA257Z.Seq
NNNNNNNNNNACTCATACACCAATCAATACAGATGATAATACCAAATGTACATTAGATGC
TTGTTCACCAAAGACTGGTGTAACTCATACACCAATCAATTGTGATGATGGAAACAAATG
TACAATCAATAGTTGTTCACCATCAGTTGGTTGTATCTCAACACCAGTTTCATGTCCAAA
ACCAAAAGATAAATGTTCAATCTCTCAATGTGATTCAGCCAAAGGTTGCATCGAAGTCCC
AATGAATTGTACCTCTGATAAATGTAATGAAGCATCATGTTGTGATGGTGTTTGTACCTC
AAAACCAATTAGCTGTCCAAAACCAAAGAATAAGTGTCAAGTTGCAAAATGTGATTTAAT
TAAAGGTTGTACCGTCTCAAATGTAGTATGTGATGATGGTAATGCTTGTACCGAAGATTC
ATGTTGTTCAGACACTGGTAAATGTCAATTCGAACCAATCAAACTTCCAAAGAATAAAAA
CAAATGTATCATTTCAAAATGTGATCCAATTAAAGGTACAATCACCAACAGTACCGTAAA
CTGTGAATGTGATGACCTTTGTAACATTGGTGAATGTTGTGAAGATACAGGAAAATGTAA
TTACAGACAAAAAGATTGTGATGATAATAATCCAAAAACAGCTGATAGTTGCGATTCCAA
GACTGGTAAATGTATTAACAAACCATATAATGTTATCACAAGTGGTTCTAATTTAATCTC
TGGTTTAATTGGTGGTCTCATTGGTGGTGGTACAGGAGATAAAGGNATTGCAAAACTGTA
AAAATTAAANA
Length of 3' end seq. 791
Connected seq. ID SHA257P
Connected seq. 
>SHA257P.Seq
ATGTACCGTAGACTCATGTTCAAATTCAACCGGTTGTGTAAACACTCCAGTCAATGTTGA
TGATAATAATCCATGTACTGTAGATGCTTGTACCAAATCAACAGGTGTCACACATACCCC
AGTAAATGTAGATGATAACAACAAATGTACAATTGATGCATGTACCAAAGAAGGTGGTGT
AACTCATACTCCAGTCAACACTGATGATAACAATGCCTGTACAATTGATTCTTGTTCACC
ATCAACNGGTATTTCCCACACCCCAATCAATTGTGATGATAAAAANGCCTGTACAGTTGA
TTCATGTTCAAATTCAACTGGTTGTGTAAATACTCCAATTNNCTGTGACGATAATAATCC
ATGTACTGTNGATTCTTGCNATGACTTAACTGGTNGTTGNNATACTCCAATCAATGTTGA
TGATAATAANNCATGTACCATCGATGCCTGTACTAAATCAACAGGTGNTACTCATACCCC
AGTCAATGNNGATGATAATAACAAATGTACAATTGATACNTGTACCAAAGAAGGTGGTGT
AACTCATACTCCACTCAATACTGATGATAACAATGCCTGTACCCTTGATTCCTGCTCACC
ATCAACTGGTGTNTCCCATACCCCAATNNACTGTGATGATAATAATANATGNACTGTANA
TTCATGTTNAAACTCAACTGGGGGNCCGAATACTCCAATTAACTGGGGACGAAAGNAANC
CCTGTNCCGGAAACTCATGCCATNAANCACNGGGGTGGGTAACNCTCNCTCAATGGTGGT
GANAAAANNCATGNCCCGTCNATNCCTG----------ACTCATACACCAATCAATACAG
ATGATAATACCAAATGTACATTAGATGCTTGTTCACCAAAGACTGGTGTAACTCATACAC
CAATCAATTGTGATGATGGAAACAAATGTACAATCAATAGTTGTTCACCATCAGTTGGTT
GTATCTCAACACCAGTTTCATGTCCAAAACCAAAAGATAAATGTTCAATCTCTCAATGTG
ATTCAGCCAAAGGTTGCATCGAAGTCCCAATGAATTGTACCTCTGATAAATGTAATGAAG
CATCATGTTGTGATGGTGTTTGTACCTCAAAACCAATTAGCTGTCCAAAACCAAAGAATA
AGTGTCAAGTTGCAAAATGTGATTTAATTAAAGGTTGTACCGTCTCAAATGTAGTATGTG
ATGATGGTAATGCTTGTACCGAAGATTCATGTTGTTCAGACACTGGTAAATGTCAATTCG
AACCAATCAAACTTCCAAAGAATAAAAACAAATGTATCATTTCAAAATGTGATCCAATTA
AAGGTACAATCACCAACAGTACCGTAAACTGTGAATGTGATGACCTTTGTAACATTGGTG
AATGTTGTGAAGATACAGGAAAATGTAATTACAGACAAAAAGATTGTGATGATAATAATC
CAAAAACAGCTGATAGTTGCGATTCCAAGACTGGTAAATGTATTAACAAACCATATAATG
TTATCACAAGTGGTTCTAATTTAATCTCTGGTTTAATTGGTGGTCTCATTGGTGGTGGTA
CAGGAGATAAAGGNATTGCAAAACTGTAAAAATTAAANA
Length of connected seq. 1589
Full length Seq ID -
Full length Seq. -
Length of full length seq. -