>SHG223 (SHG223Q) /CSM/SH/SHG2-A/SHG223Q.Seq.d/
GGAGGCTTGTCGCAATGCTGACGTGCAAATCGCTTGTCTAACTTGGGTATAGGGGCGAAA
GACTAATCGAACAACCTAGTAGCTGGTTCCTTCCGAAGTTTCCCTCAGGATAGCTGGAGC
AGTATTCTAGTTCCATCTTGTAAAGACAATGATTAGCAGTTTCGGGGGCGTAATGCTCTC
AGCTGATTCTCAAACTCTGAACGGGTGGGTATCATTTTAATTCACTTAATTGGATTTTAA
AATTAAATTGCACATGTGCAATGAAAAATAGGAGCTCTTAGTGGGCCATTTTTGGTAAGC
AGAACTGGCGATGTGGGTTGAACCAAATATTGGGATAAGACGTCTAACATTCACTAATAG
ATACCACAAAAGGTGTTAGTTCATTAAGACAGCAGGACGGTGGCCATGGAAGTCGGTATC
CGCTAAGGAGTGTGTAACAACTCACCTGCCAAATGGACTAGCCCTGAAAATGGATGACGC
TAGCAGTGGATGGTCGATGCCCAATCGTTAAAAGAAGTGATAATACTTTTAACGTGTAGG
AAGGCGTGAAGGTAACGTAGAAGCTTGAATGTGAATTCGAGTGGAGTTGTCTTTAGTGCA
GATCTTGATGGTAGTAGCAAATATTCAAAAGAATTTACTTTGAAGGCXXXXXXXXXXCTA
GAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGCTCATAGCG
ACGCTGCTTTTTGATCCTTCGATGTCGGCTCTTCTTATCATTGTGAAGCAGAATTCACAA
AGTGTTGGATTGTTCACCCACTAACAAGGAACGTGAGCTGGGTTTAGACCGTCGTGAGAC
AGGTTAGTTTTACCCTACTGTTGTCAATTGTTTGCGTAATAGTAGCATGATTTAGTACGA
GAGGAACTGTCATGCCGGATCACTGGTCTGTAGGTTTATTTGACAAAATAGTGACCTGCC
GCTACCATCCGTTGGATAATGGCTGAACGCCTCTAAGTCAGAATCCATTCTAGAAACGCA
AACCAAATGCTTTAGAGTGTGAATGTTGTAGGTAACATTAGGTTGTTGGTGGGGGACCAC
TTTCAACTTTAAACCATATGATTAATCGCTGTTACACTGCAGTTTCCTTCCGGTTATTGT
GGTGGGTGGCTAAATTCTAATTTATATCCTCGTTCCGCTCAACTCTTCGATTGTAGACGA
CTATCAAATGAACTAGGTGCTGTAAGCTTCCGAGTAGCGTTCAGTTACGAGGGGTTGAGG
CTTTTCCATTAGTTCTTAAAATTAAATATCAATACAATTAAAAAAAGTTAAAATTAAATT
GTAAAATCAATT

gglsqc*ranrlsnlgigakd*snnlvagsfrsfpqdswssilvpsckdnd*qfrgrnal
s*fsnservgiilihligf*n*iahvq*kigalsgpflvsrtgdvg*tkywdktsnih**
ipqkvlvh*dsrtvamevgir*gvcnnspakwtspeng*r*qwmvdaqslkeviilltcr
ka*r*RRSLNVNSSGVVFSADLDGSSKYSKEFTLK---

---RGVRKVTTGITGLWQPSAHSDAAF*sfdvgssyhceaeftkcwivhpltrnvswv*t
vvrqvsftlllsivcviva*fstrgtvmpdhwsvglfdkivtcryhpldng*tplsqnpf
*krkpnalececcr*h*vvgggplstlnhminrcytavsfrllwwvakf*fissfrstlr
l*ttik*trccklpssvqlrgveafplvlkikyqyn*kklklnckin

Frame A:
gglsqc*ranrlsnlgigakd*snnlvagsfrsfpqdswssilvpsckdnd*qfrgrnal
s*fsnservgiilihligf*n*iahvq*kigalsgpflvsrtgdvg*tkywdktsnih**
ipqkvlvh*dsrtvamevgir*gvcnnspakwtspeng*r*qwmvdaqslkeviilltcr
ka*r*RRSLNVNSSGVVFSADLDGSSKYSKEFTLK---

---levseklpqg*lacgsqaliatllfdpsmsalliivkqnsqsvglfth*qgt*agfr
ps*drlvlpyccqlfa***hdlvreelscritgl*vyltk**paatirwimaerl*vrih
srnanqml*svnvvgnirllvgdhfql*ti*liavtlqfpsgycggwlnsnlyprsaqlf
dcrrlsnelgavsfrvafsyeglrlfh*flklnintikks*n*ivksi

Frame B:
eacrnadvqiacltwv*grktnrtt**lvpsevslriagavf*fhlvktmissfggvmls
adsqtlngwvsf*ft*ldfkiklhmcnek*ellvghfw*aelamwvepnigirrltftnr
yhkrc*fiktagrwpwksvsakecvtthlpnglalkmddassgwsmpnr*kk**yf*rvg
rregnvea*m*irvelslvqilmvvaniqknll*r---

---*rcqksyhrdnwlvaakrs*rrcflilrcrlflsl*srihkvldcsptnkerelgld
rretg*fyptvvnclrnssmi*yernchagslvcrfi*qnsdlplpsvg*wlnasksesi
letqtkcfrv*ml*vtlgcwwgttfnfkpyd*sllhcsflpvivvgg*iliyilvplnss
ivddyqmn*vl*ase*rsvtrg*gfsiss*n*isiqlkkvkikl*nq

Frame C:
rlvamltckslv*lgyrgerlieqpsswflpkfpsg*leqysssil*rq*lavsga*csq
lilkl*tggyhfnslnwilklnctcamknrss*waifgkqnwrcglnqilg*dv*hslid
ttkgvsslrqqdgghgsryplrsv*qltcqmd*p*kwmtlavdgrcpivkrsdntfnv*e
gvkvt*klecefewscl*crs*w**qifkriyfeg---

---RGVRKVTTGITGLWQPSAHSDAAF*sfdvgssyhceaeftkcwivhpltrnvswv*t
vvrqvsftlllsivcviva*fstrgtvmpdhwsvglfdkivtcryhpldng*tplsqnpf
*krkpnalececcr*h*vvgggplstlnhminrcytavsfrllwwvakf*fissfrstlr
l*ttik*trccklpssvqlrgveafplvlkikyqyn*kklklnckin

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHG223 (SHG223Q) /CSM/SH/SHG2-A/SHG223Q.Seq.d/                       2539   0.0
SHH833 (SHH833Q) /CSM/SH/SHH8-B/SHH833Q.Seq.d/                       1273   0.0
AHE287 (AHE287Q) /CSM/AH/AHE2-D/AHE287Q.Seq.d/                       1233   0.0
VHK875 (VHK875Q) /CSM/VH/VHK8-D/VHK875Q.Seq.d/                       1231   0.0
VHD686 (VHD686Q) /CSM/VH/VHD6-D/VHD686Q.Seq.d/                       1231   0.0
VHD592 (VHD592Q) /CSM/VH/VHD5-D/VHD592Q.Seq.d/                       1231   0.0
SHA880 (SHA880Q) /CSM/SH/SHA8-D/SHA880Q.Seq.d/                       1231   0.0
SFC338 (SFC338Q) /CSM/SF/SFC3-B/SFC338Q.Seq.d/                       1231   0.0
CHI557 (CHI557Q) /CSM/CH/CHI5-C/CHI557Q.Seq.d/                       1231   0.0
AHI666 (AHI666Q) /CSM/AH/AHI6-C/AHI666Q.Seq.d/                       1231   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY171066|AY171066.1 Dictyostelium discoideum extrachromosomal palindromic ribosomal RNA gene sequence, central segment.  1283   0.0    1
X00601|X00601.1 Dictyostelium discoideum ribosomal RNA gene.     1273   0.0    1
DQ340385|DQ340385.1 Dictyostelium citrinum 17S ribosomal RNA, 5.8S ribosomal RNA, 26S ribosomal RNA, and 5S ribosomal RNA genes, complete sequence.  1243   0.0    1
DQ340386|DQ340386.1 Dictyostelium purpureum 17S ribosomal RNA, 5.8S ribosomal RNA, 26S ribosomal RNA, and 5S ribosomal RNA genes, complete sequence.   648   0.0    7
DQ340387|DQ340387.1 Dictyostelium polycephalum 17S ribosomal RNA, 5.8S ribosomal RNA, and 26S ribosomal RNA genes, complete sequence.   385   0.0    9
DQ340388|DQ340388.1 Polysphondylium pallidum 17S ribosomal RNA, 5.8S ribosomal RNA, 26S ribosomal RNA, and 5S ribosomal RNA genes, complete sequence.   355   0.0    9
AJ864475|AJ864475.1 Orpinomyces sp. OUS1 partial 18S rRNA gene, ITS1, 5.8S rRNA gene, ITS2 and partial 28S rRNA, clone V5-1.   331   0.0    7
DQ002502|DQ002502.1 Tetraplatia volitans 28S large subunit ribosomal RNA gene, partial sequence.   347   0.0    7
AF289037|AF289037.1 Guillardia theta 28S ribosomal RNA gene, complete sequence.   339   0.0    7
DQ273838|DQ273838.1 Podochytrium dentatum isolate AFTOL-ID 1539 28S ribosomal RNA gene, partial sequence.   315   0.0    7

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU430725_1(EU430725|pid:none) Cupressus sempervirens clone CYPLP...   130   1e-28
CP000497_644(CP000497|pid:none) Pichia stipitis CBS 6054 chromos...    60   9e-14
AY436773_1(AY436773|pid:none) Pyrus communis putative senescence...    75   4e-12
AK230153_1(AK230153|pid:none) Arabidopsis thaliana mRNA for hypo...    68   8e-10
EU244380_1(EU244380|pid:none) Haliotis diversicolor clone HDr4CJ...    68   8e-10
BC114377_1(BC114377|pid:none) Homo sapiens cDNA clone MGC:134704...    65   7e-09
AY810980_1(AY810980|pid:none) Schistosoma japonicum clone SJCHGC...    58   6e-07
EF192058_1(EF192058|pid:none) Trichosanthes dioica clone TDM04 p...    52   6e-05
U12823_1(U12823|pid:none) Acanthamoeba polyphaga CDC:0187:1 hemo...    45   9e-04
EU955547_1(EU955547|pid:none) Zea mays clone 1536647 hypothetica...    45   0.007

 psg: 0.89  gvh: 0.45  alm: 0.54  top: 0.33  tms: 0.00  mit: 0.44  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 24.0 %: mitochondrial
	 16.0 %: cytoplasmic
	  8.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for SHG223 is nuc

>SHG223F.Seq
GGAGGCTTGTCGCAATGCTGACGTGCAAATCGCTTGTCTAACTTGGGTATAGGGGCGAAA
GACTAATCGAACAACCTAGTAGCTGGTTCCTTCCGAAGTTTCCCTCAGGATAGCTGGAGC
AGTATTCTAGTTCCATCTTGTAAAGACAATGATTAGCAGTTTCGGGGGCGTAATGCTCTC
AGCTGATTCTCAAACTCTGAACGGGTGGGTATCATTTTAATTCACTTAATTGGATTTTAA
AATTAAATTGCACATGTGCAATGAAAAATAGGAGCTCTTAGTGGGCCATTTTTGGTAAGC
AGAACTGGCGATGTGGGTTGAACCAAATATTGGGATAAGACGTCTAACATTCACTAATAG
ATACCACAAAAGGTGTTAGTTCATTAAGACAGCAGGACGGTGGCCATGGAAGTCGGTATC
CGCTAAGGAGTGTGTAACAACTCACCTGCCAAATGGACTAGCCCTGAAAATGGATGACGC
TAGCAGTGGATGGTCGATGCCCAATCGTTAAAAGAAGTGATAATACTTTTAACGTGTAGG
AAGGCGTGAAGGTAACGTAGAAGCTTGAATGTGAATTCGAGTGGAGTTGTCTTTAGTGCA
GATCTTGATGGTAGTAGCAAATATTCAAAAGAATTTACTTTGAAGGCNNNNNNNNNN

>SHG223Z.Seq
NNNNNNNNNNCTAGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCA
AGCGCTCATAGCGACGCTGCTTTTTGATCCTTCGATGTCGGCTCTTCTTATCATTGTGAA
GCAGAATTCACAAAGTGTTGGATTGTTCACCCACTAACAAGGAACGTGAGCTGGGTTTAG
ACCGTCGTGAGACAGGTTAGTTTTACCCTACTGTTGTCAATTGTTTGCGTAATAGTAGCA
TGATTTAGTACGAGAGGAACTGTCATGCCGGATCACTGGTCTGTAGGTTTATTTGACAAA
ATAGTGACCTGCCGCTACCATCCGTTGGATAATGGCTGAACGCCTCTAAGTCAGAATCCA
TTCTAGAAACGCAAACCAAATGCTTTAGAGTGTGAATGTTGTAGGTAACATTAGGTTGTT
GGTGGGGGACCACTTTCAACTTTAAACCATATGATTAATCGCTGTTACACTGCAGTTTCC
TTCCGGTTATTGTGGTGGGTGGCTAAATTCTAATTTATATCCTCGTTCCGCTCAACTCTT
CGATTGTAGACGACTATCAAATGAACTAGGTGCTGTAAGCTTCCGAGTAGCGTTCAGTTA
CGAGGGGTTGAGGCTTTTCCATTAGTTCTTAAAATTAAATATCAATACAATTAAAAAAAG
TTAAAATTAAATTGTAAAATCAATT

>SHG223P.Seq
GGAGGCTTGTCGCAATGCTGACGTGCAAATCGCTTGTCTAACTTGGGTATAGGGGCGAAA
GACTAATCGAACAACCTAGTAGCTGGTTCCTTCCGAAGTTTCCCTCAGGATAGCTGGAGC
AGTATTCTAGTTCCATCTTGTAAAGACAATGATTAGCAGTTTCGGGGGCGTAATGCTCTC
AGCTGATTCTCAAACTCTGAACGGGTGGGTATCATTTTAATTCACTTAATTGGATTTTAA
AATTAAATTGCACATGTGCAATGAAAAATAGGAGCTCTTAGTGGGCCATTTTTGGTAAGC
AGAACTGGCGATGTGGGTTGAACCAAATATTGGGATAAGACGTCTAACATTCACTAATAG
ATACCACAAAAGGTGTTAGTTCATTAAGACAGCAGGACGGTGGCCATGGAAGTCGGTATC
CGCTAAGGAGTGTGTAACAACTCACCTGCCAAATGGACTAGCCCTGAAAATGGATGACGC
TAGCAGTGGATGGTCGATGCCCAATCGTTAAAAGAAGTGATAATACTTTTAACGTGTAGG
AAGGCGTGAAGGTAACGTAGAAGCTTGAATGTGAATTCGAGTGGAGTTGTCTTTAGTGCA
GATCTTGATGGTAGTAGCAAATATTCAAAAGAATTTACTTTGAAGGC----------CTA
GAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGCTCATAGCG
ACGCTGCTTTTTGATCCTTCGATGTCGGCTCTTCTTATCATTGTGAAGCAGAATTCACAA
AGTGTTGGATTGTTCACCCACTAACAAGGAACGTGAGCTGGGTTTAGACCGTCGTGAGAC
AGGTTAGTTTTACCCTACTGTTGTCAATTGTTTGCGTAATAGTAGCATGATTTAGTACGA
GAGGAACTGTCATGCCGGATCACTGGTCTGTAGGTTTATTTGACAAAATAGTGACCTGCC
GCTACCATCCGTTGGATAATGGCTGAACGCCTCTAAGTCAGAATCCATTCTAGAAACGCA
AACCAAATGCTTTAGAGTGTGAATGTTGTAGGTAACATTAGGTTGTTGGTGGGGGACCAC
TTTCAACTTTAAACCATATGATTAATCGCTGTTACACTGCAGTTTCCTTCCGGTTATTGT
GGTGGGTGGCTAAATTCTAATTTATATCCTCGTTCCGCTCAACTCTTCGATTGTAGACGA
CTATCAAATGAACTAGGTGCTGTAAGCTTCCGAGTAGCGTTCAGTTACGAGGGGTTGAGG
CTTTTCCATTAGTTCTTAAAATTAAATATCAATACAATTAAAAAAAGTTAAAATTAAATT
GTAAAATCAATT