>SHI292 (SHI292Q) /CSM/SH/SHI2-D/SHI292Q.Seq.d/
ATTTTTTTATAATTTACATTAGTATTTCTATTATTTTCCATTAATTTGAAATTTTATTTA
AATAAAGATAAAATGCCCCAAAATAAAATTCAAACAATAAAACCAGCTCATTTTTTAAGA
GATAATCTTCTTAACTATCCAGAATCAAAAATATTTAGAGAATTGATTCAAAATGCAGAA
GATGCTCGAGCTGATACAGTCATAATAAAGGTTGATGAAGGCTCATATCCAAATAATGGG
TTATTAATTTCAAATGATAACCAAGAAATTCAAAGTTCAAAAGAATTATTAGGTCCATCA
ATTTTAATTTATAATAATGCAACTTTTCAAAAACATGATTGGGAAGGAATTTGTCAAATT
AGTCAAGGTAGTAAAAAAGAAAATCTAAAAAGTGTTGGCAATTTCGGTTTAGGTTGGAAT
AGTGTTTATCATATAACAGATAATCCAATTGTTTATAGTGGTAATAATGTTTGGTTTGCA
GATCCAAATGAGAGAATGAGTGGTGGTTTATTCTTTGATTTGGATGATGAAGATGATTAT
AACAAATGTCTTAGTTTTTTAGAGCCATTCATTCAATTTAAAGAATCAAAXXXXXXXXXX

ifl*FTLVFLLFSINLKFYLNKDKMPQNKIQTIKPAHFLRDNLLNYPESKIFRELIQNAE
DARADTVIIKVDEGSYPNNGLLISNDNQEIQSSKELLGPSILIYNNATFQKHDWEGICQI
SQGSKKENLKSVGNFGLGWNSVYHITDNPIVYSGNNVWFADPNERMSGGLFFDLDDEDDY
NKCLSFLEPFIQFKES---

Frame A:
ifl*FTLVFLLFSINLKFYLNKDKMPQNKIQTIKPAHFLRDNLLNYPESKIFRELIQNAE
DARADTVIIKVDEGSYPNNGLLISNDNQEIQSSKELLGPSILIYNNATFQKHDWEGICQI
SQGSKKENLKSVGNFGLGWNSVYHITDNPIVYSGNNVWFADPNERMSGGLFFDLDDEDDY
NKCLSFLEPFIQFKES---


Frame B:
ffynlh*yfyyfpli*nfi*ikikcpkikfkq*nqlif*eiifltiqnqkylen*fkmqk
mleliqs**rlmkahiqimgy*fqmitkkfkvqkny*vhqf*fiimqlfknmigkefvkl
vkvvkkki*kvlaisv*vgivfii*qiiqlfivvimfglqiqmre*vvvysliwmmkmii
tnvlvf*shsfnlknq---


Frame C:
ffiiyisisiifh*feilfk*r*napk*nsnnktssffkr*ss*lsrikni*ridskcrr
css*yshnkg**rlisk*wvinfk**prnskfkriirsinfnl**cnfskt*lgrnlsn*
sr**krkskkcwqfrfrle*clsynr*sncl*w**clvcrsk*enewwfil*fg**r*l*
qms*ffraihsi*ri---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHI292 (SHI292Q) /CSM/SH/SHI2-D/SHI292Q.Seq.d/                        618   e-176
VHN647 (VHN647Q) /CSM/VH/VHN6-B/VHN647Q.Seq.d/                        561   e-159
VHK810 (VHK810Q) /CSM/VH/VHK8-A/VHK810Q.Seq.d/                        561   e-159
VHD239 (VHD239Q) /CSM/VH/VHD2-B/VHD239Q.Seq.d/                        561   e-159
VHC102 (VHC102Q) /CSM/VH/VHC1-A/VHC102Q.Seq.d/                        561   e-159
VHB767 (VHB767Q) /CSM/VH/VHB7-C/VHB767Q.Seq.d/                        561   e-159
VHO809 (VHO809Q) /CSM/VH/VHO8-A/VHO809Q.Seq.d/                        553   e-156
VHE271 (VHE271Q) /CSM/VH/VHE2-C/VHE271Q.Seq.d/                        553   e-156
VHE123 (VHE123Q) /CSM/VH/VHE1-A/VHE123Q.Seq.d/                        553   e-156
AHQ220 (AHQ220Q) /CSM/AH/AHQ2-A/AHQ220Q.Seq.d/                        553   e-156

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL034557|AL034557.8 Plasmodium falciparum MAL4P1.                  36   0.008  8
DT516046|DT516046.1 WS02430.B21_D24 PTxD-ICC-N-A-14 Populus trichocarpa x Populus deltoides cDNA clone WS02430_D24 3', mRNA sequence.    36   0.046  2
AB024723|AB024723.1 Colwellia psychroerythraea gene for Na-translocating NADH-quinone reductase, partial cds.    38   0.15   2
AE002149|AE002149.1 Ureaplasma urealyticum section 50 of 59 of the complete genome.    42   0.27   4
AE009951|AE009951.2 Fusobacterium nucleatum subsp. nucleatum ATCC 25586, complete genome.    40   0.39   13
BX005076|BX005076.13 Zebrafish DNA sequence from clone DKEY-7N16 in linkage group 25.    38   0.52   4
AX344569|AX344569.1 Sequence 20 from Patent WO0200932.             38   0.68   9
AC184703|AC184703.1 Zea mays chromosome UNK clone CH201-6M3; ZMMBBc0006M03, *** SEQUENCING IN PROGRESS ***, 32 unordered pieces.    46   0.93   1
Z93778|Z93778.1 Caenorhabditis elegans Cosmid C31H5.               46   0.93   1
BS000088|BS000088.1 Pan troglodytes chromosome 22 clone:RP43-078B15, map 22, complete sequences.    46   0.93   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9NZJ4) RecName: Full=Sacsin;  &AL157766_4(AL157766|pid:none)        106   4e-22
BC138482_1(BC138482|pid:none) Mus musculus sacsin, mRNA (cDNA cl...   104   2e-21
(Q9JLC8) RecName: Full=Sacsin;                                        104   2e-21
BC171956_1(BC171956|pid:none) Mus musculus cDNA clone MGC:198661...   104   2e-21
BC142916_1(BC142916|pid:none) Danio rerio hypothetical LOC555303...   100   5e-20
AB006708_10(AB006708|pid:none) Arabidopsis thaliana genomic DNA,...    84   2e-15
AL732351_6(AL732351|pid:none) Oryza sativa genomic DNA, chromoso...    80   3e-14
AL606457_18(AL606457|pid:none) Oryza sativa genomic DNA, chromos...    80   3e-14
CR749427_1(CR749427|pid:none) Homo sapiens mRNA; cDNA DKFZp686B1...    73   5e-12
AE017282_697(AE017282|pid:none) Methylococcus capsulatus str. Ba...    55   2e-06

 psg: 1.00  gvh: 0.39  alm: 0.37  top: 0.67  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	 16.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for SHI292 is cyt

>SHI292F.Seq
ATTTTTTTATAATTTACATTAGTATTTCTATTATTTTCCATTAATTTGAAATTTTATTTA
AATAAAGATAAAATGCCCCAAAATAAAATTCAAACAATAAAACCAGCTCATTTTTTAAGA
GATAATCTTCTTAACTATCCAGAATCAAAAATATTTAGAGAATTGATTCAAAATGCAGAA
GATGCTCGAGCTGATACAGTCATAATAAAGGTTGATGAAGGCTCATATCCAAATAATGGG
TTATTAATTTCAAATGATAACCAAGAAATTCAAAGTTCAAAAGAATTATTAGGTCCATCA
ATTTTAATTTATAATAATGCAACTTTTCAAAAACATGATTGGGAAGGAATTTGTCAAATT
AGTCAAGGTAGTAAAAAAGAAAATCTAAAAAGTGTTGGCAATTTCGGTTTAGGTTGGAAT
AGTGTTTATCATATAACAGATAATCCAATTGTTTATAGTGGTAATAATGTTTGGTTTGCA
GATCCAAATGAGAGAATGAGTGGTGGTTTATTCTTTGATTTGGATGATGAAGATGATTAT
AACAAATGTCTTAGTTTTTTAGAGCCATTCATTCAATTTAAAGAATCAAANNNNNNNNNN