>SHI734 (SHI734Q) /CSM/SH/SHI7-B/SHI734Q.Seq.d/
AAAAAGACATAAATATATAATAAATAATGTCAGAACCAAAACCAACTCAACCAATCAAAG
AGATTGATGAGGAAACTAAAAGAAAGAATGAAGAGAAAAAGAAAGCCAAAGAGGCAGAGA
AAGCAGAGAAATTAGCAAAAATGAAAGAAAAGGAAGCTAAACAAGCAGCAGCAAAAGAAA
AAGAATTACAAAATAAACTTAAAAAAGAACAAGAGAAAAAAGAAAAAGAAGCTGAAGAAA
AAGAAAAACAAGAAGCTGAAGAAAAAATTGTTAGAGAACATATTGAAAAAATTTTAAAAA
CCCCAAAGGGTGAAAAGAAAGATGTTTCAAGTTTATTATCATCATATCATCCAACAGCAG
TTGAATCAATTTGGTATGATTATTGGTTAGATAATGGATATTTTTCACCAGAGAAACAAA
TGGAGATTCAACCACATGTAGTTAAAGATAAAAAGTTTGTAATTGTTATTCCACCACCAA
ATGTTACAGGTAGTTTACATTTAGGACATGCATTAACCAATTCAATTCAAGATGCAGTTG
TTCGTTATCATCGTATGAAGGGGTGAAGTTTGTTTATGGGTACCAGGTACAGATCATGCA
GGTATTGCAACACAAGTAGTTGTAGAXXXXXXXXXX

kdini**IMSEPKPTQPIKEIDEETKRKNEEKKKAKEAEKAEKLAKMKEKEAKQAAAKEK
ELQNKLKKEQEKKEKEAEEKEKQEAEEKIVREHIEKILKTPKGEKKDVSSLLSSYHPTAV
ESIWYDYWLDNGYFSPEKQMEIQPHVVKDKKFVIVIPPPNVTGSLHLGHALTNSIQDAVV
RYHRMKG*slfmgtryrscrycntssc---

Frame A:
kkt*iynk*cqnqnqlnqskrlmrklkermkrkrkpkrqrkqrn*qk*kkrklnkqqqkk
knykinlkknkrkkkkklkkkknkklkkkllenilkkf*kpqrvkrkmfqvyyhhiiqqq
lnqfgmiig*imdifhqrnkwrfnhm*lkiksl*llfhhqmlqvvyi*dmh*piqfkmql
fviiv*rgevclwvpgtdhagiatqvvv---


Frame B:
krhkyiinnvrtktnstnqrd**gn*kke*rekesqrgresreisknerkgs*tssskrk
ritk*t*krtrekrkrs*rkrktrs*rknc*rty*knfknpkg*kercfkfiiiissnss
*inlv*llvr*wifftretngdsttcs*r*kvcncysttkcyr*ftfrtcinqfnsrcsc
slssyegvkfvygyqvqimqvlqhk*l*---


Frame C:
kdini**IMSEPKPTQPIKEIDEETKRKNEEKKKAKEAEKAEKLAKMKEKEAKQAAAKEK
ELQNKLKKEQEKKEKEAEEKEKQEAEEKIVREHIEKILKTPKGEKKDVSSLLSSYHPTAV
ESIWYDYWLDNGYFSPEKQMEIQPHVVKDKKFVIVIPPPNVTGSLHLGHALTNSIQDAVV
RYHRMKG*slfmgtryrscrycntssc---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHI734 (SHI734Q) /CSM/SH/SHI7-B/SHI734Q.Seq.d/                        607   e-173
VHP768 (VHP768Q) /CSM/VH/VHP7-C/VHP768Q.Seq.d/                        591   e-168
VHM840 (VHM840Q) /CSM/VH/VHM8-B/VHM840Q.Seq.d/                        591   e-168
VHL308 (VHL308Q) /CSM/VH/VHL3-A/VHL308Q.Seq.d/                        591   e-168
VHA717 (VHA717Q) /CSM/VH/VHA7-A/VHA717Q.Seq.d/                        591   e-168
SHI109 (SHI109Q) /CSM/SH/SHI1-A/SHI109Q.Seq.d/                        591   e-168
SHA541 (SHA541Q) /CSM/SH/SHA5-B/SHA541Q.Seq.d/                        591   e-168
CHO422 (CHO422Q) /CSM/CH/CHO4-A/CHO422Q.Seq.d/                        591   e-168
CHP511 (CHP511Q) /CSM/CH/CHP5-A/CHP511Q.Seq.d/                        587   e-167
VHP692 (VHP692Q) /CSM/VH/VHP6-D/VHP692Q.Seq.d/                        583   e-165

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strainAX4, complete sequence.   480   0.0    3
AB092433|AB092433.1 Entamoeba histolytica mRNA for valyl tRNA synthetase, partial cds.    96   4e-27  2
BH161129|BH161129.1 ENTSE35TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    84   1e-23  2
BH159895|BH159895.1 ENTRU27TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    72   4e-20  2
AZ550882|AZ550882.1 ENTEC77TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    84   9e-20  3
AZ551677|AZ551677.1 ENTDS20TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    84   5e-12  1
DT618861| ENSANGP00000010240 [Anopheles gambiae str. PEST], mRNA sequence.    52   4e-09  2
CV464811|CV464811.1 taj26d03.y1 Hydra EST UCI 5 ALP Hydra magnipapillata cDNA 5' similar to SW:SYV_FUGRU P49696 VALYL-TRNA SYNTHETASE ;, mRNA sequence.    52   4e-09  2
CZ531779|CZ531779.1 SRAA-aac74g10.b1 Strongyloides ratti whole genome shotgun library (SRAAGSS 004) Strongyloides ratti genomic, genomic survey sequence.    46   4e-06  2
AX414269|AX414269.1 Sequence 1260 from Patent WO0228891.           52   1e-05  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86KU2) RecName: Full=Probable valyl-tRNA synthetase, cytoplasm...   207   7e-61
AB092433_1(AB092433|pid:none) Entamoeba histolytica mRNA for val...   106   7e-28
AE013599_1725(AE013599|pid:none) Drosophila melanogaster chromos...   104   3e-27
CR954203_287(CR954203|pid:none) Ostreococcus tauri strain OTTH05...   103   2e-26
EU282356_1(EU282356|pid:none) Sus scrofa valyl-tRNA synthetase (...   100   7e-26
(P26640) RecName: Full=Valyl-tRNA synthetase;          EC=6.1.1....    99   1e-25
BC012808_1(BC012808|pid:none) Homo sapiens valyl-tRNA synthetase...    99   1e-25
AF087141_1(AF087141|pid:none) Mus musculus uncharacterized long ...    99   1e-25
(Q04462) RecName: Full=Valyl-tRNA synthetase;          EC=6.1.1....    99   1e-25
BC053703_1(BC053703|pid:none) Mus musculus valyl-tRNA synthetase...    99   1e-25

 psg: 0.61  gvh: 0.07  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.12  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for SHI734 is nuc

>SHI734F.Seq
AAAAAGACATAAATATATAATAAATAATGTCAGAACCAAAACCAACTCAACCAATCAAAG
AGATTGATGAGGAAACTAAAAGAAAGAATGAAGAGAAAAAGAAAGCCAAAGAGGCAGAGA
AAGCAGAGAAATTAGCAAAAATGAAAGAAAAGGAAGCTAAACAAGCAGCAGCAAAAGAAA
AAGAATTACAAAATAAACTTAAAAAAGAACAAGAGAAAAAAGAAAAAGAAGCTGAAGAAA
AAGAAAAACAAGAAGCTGAAGAAAAAATTGTTAGAGAACATATTGAAAAAATTTTAAAAA
CCCCAAAGGGTGAAAAGAAAGATGTTTCAAGTTTATTATCATCATATCATCCAACAGCAG
TTGAATCAATTTGGTATGATTATTGGTTAGATAATGGATATTTTTCACCAGAGAAACAAA
TGGAGATTCAACCACATGTAGTTAAAGATAAAAAGTTTGTAATTGTTATTCCACCACCAA
ATGTTACAGGTAGTTTACATTTAGGACATGCATTAACCAATTCAATTCAAGATGCAGTTG
TTCGTTATCATCGTATGAAGGGGTGAAGTTTGTTTATGGGTACCAGGTACAGATCATGCA
GGTATTGCAACACAAGTAGTTGTAGANNNNNNNNNN