>SHI777 (SHI777Q) /CSM/SH/SHI7-D/SHI777Q.Seq.d/
CATTCCCATTGACAAGTACAACATTTCCAAAGATTACCAAAGGTTCATGGTCAAGCCAAG
AGATCTATTCAACTAGAGATATCAAAGAGATCATTCAACATGCTAAAGAATATGGTATTC
GTGTGGAGTTAGAGATTGATATGCCTGGACATGCCTATAGTTGGGGCATTGGCTATCCAA
GTGTTTTGCCAGCAAATTTCAGTCACTCAATTCAGTGTCAACCATCCATCTGTACTAATA
AATAATAATAAATAAAAAAAAATAAATAAAAAAAATAAAAAAATATTTATTTAAAAATAA
AAAAAAAANTTTAAAAAAAAAAXXXXXXXXXX

FPLTSTTFPKITKGSWSSQEIYSTRDIKEIIQHAKEYGIRVELEIDMPGHAYSWGIGYPS
VLPANFSHSIQCQPSICTNK***ikknk*kk*kniylkikkkx*kk---

Frame A:
hsh*qvqhfqrlpkvhgqakrsiqleiskrsfnmlknmvfvws*rlicldmpivgalaiq
vfcqqisvtqfsvnhpsvlinnnk*kkinkknkkifi*k*kkxfkkk---


Frame B:
ipidkyniskdyqrfmvkprdlfn*ryqrdhstc*riwyscgvrd*yawtcl*lghwlsk
cfaskfqslnsvstihly**iiinkkk*ikkikkylfknkkkxlkk---


Frame C:
FPLTSTTFPKITKGSWSSQEIYSTRDIKEIIQHAKEYGIRVELEIDMPGHAYSWGIGYPS
VLPANFSHSIQCQPSICTNK***ikknk*kk*kniylkikkkx*kk---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHI777 (SHI777Q) /CSM/SH/SHI7-D/SHI777Q.Seq.d/                        466   e-130
SLE592 (SLE592Q) /CSM/SL/SLE5-D/SLE592Q.Seq.d/                        440   e-123
SLD309 (SLD309Q) /CSM/SL/SLD3-A/SLD309Q.Seq.d/                        420   e-117
AFA589 (AFA589Q) /CSM/AF/AFA5-D/AFA589Q.Seq.d/                         38   0.081
SHD201 (SHD201Q) /CSM/SH/SHD2-A/SHD201Q.Seq.d/                         36   0.32
SLF416 (SLF416Q) /CSM/SL/SLF4-A/SLF416Q.Seq.d/                         34   1.3
FC-AB03 (FC-AB03Q) /CSM/FC/FC-AB/FC-AB03Q.Seq.d/                       34   1.3
VSH873 (VSH873Q) /CSM/VS/VSH8-D/VSH873Q.Seq.d/                         32   5.0
VHQ407 (VHQ407Q) /CSM/VH/VHQ4-A/VHQ407Q.Seq.d/                         32   5.0
VHQ360 (VHQ360Q) /CSM/VH/VHQ3-C/VHQ360Q.Seq.d/                         32   5.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BW994976|BW994976.1 Cryptomeria japonica cDNA, clone: CM03272, 3' end, expressed inmale cone.    32   1.4    2
CT607112|CT607112.1 Danio rerio EST, clone ZF_mu_173e24 5'.        42   7.8    1
AC171937|AC171937.3 Bos taurus clone CH240-274P21, *** SEQUENCING IN PROGRESS ***, 37 unordered pieces.    42   7.8    1
AC163140|AC163140.2 Bos taurus clone CH240-80C17, *** SEQUENCING IN PROGRESS ***, 33 unordered pieces.    42   7.8    1
AC152808|AC152808.3 Bos taurus clone CH240-9G20, *** SEQUENCING IN PROGRESS ***, 26 unordered pieces.    42   7.8    1
CC986955|CC986955.1 ZUAE002TV ZM_3.0_4.0_KB Zea mays genomic clone ZMMBPa0038A03, genomic survey sequence.    42   7.8    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54K56) RecName: Full=Beta-hexosaminidase subunit B2;          ...   155   3e-37
(Q54K55) RecName: Full=Beta-hexosaminidase subunit B1;          ...    92   5e-18
(P13723) RecName: Full=Beta-hexosaminidase subunit A1;          ...    78   1e-13
AP003407_2(AP003407|pid:none) Oryza sativa Japonica Group genomi...    74   2e-12
EF676594_1(EF676594|pid:none) Picea sitchensis clone WS02743_M24...    72   4e-12
AC078977_6(AC078977|pid:none) Oryza sativa (japonica cultivar-gr...    72   4e-12
AM493720_1(AM493720|pid:none) Arabidopsis thaliana mRNA for beta...    72   6e-12
EU955869_1(EU955869|pid:none) Zea mays clone 1549801 beta-hexosa...    72   6e-12
AY128283_1(AY128283|pid:none) Arabidopsis thaliana At1g65600/F5I...    70   3e-11
EU442572_1(EU442572|pid:none) Sus scrofa hexosaminidase A alpha ...    69   4e-11

 psg: 0.76  gvh: 0.39  alm: 0.54  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 28.0 %: nuclear
	 24.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for SHI777 is cyt

>SHI777F.Seq
CATTCCCATTGACAAGTACAACATTTCCAAAGATTACCAAAGGTTCATGGTCAAGCCAAG
AGATCTATTCAACTAGAGATATCAAAGAGATCATTCAACATGCTAAAGAATATGGTATTC
GTGTGGAGTTAGAGATTGATATGCCTGGACATGCCTATAGTTGGGGCATTGGCTATCCAA
GTGTTTTGCCAGCAAATTTCAGTCACTCAATTCAGTGTCAACCATCCATCTGTACTAATA
AATAATAATAAATAAAAAAAAATAAATAAAAAAAATAAAAAAATATTTATTTAAAAATAA
AAAAAAAANTTTAAAAAAAAAANNNNNNNNNN