>AFB241 (AFB241Q) /CSM/AF/AFB2-B/AFB241Q.Seq.d/
ATAATNAACTTTAAAGAAAGAATAAACAACCATGGGTAAAGAGAAAACTCACATTAACAT
TGTCGTCATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAA
ATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTATGAAAAGGAAGCTTCTGAAATGGG
TAAACAATCCTTCAAATACGCCTGGGTTATGGATAAATTAAAGGCTGAACGTGAAAGAGG
TATCACCATCGATATTGCTTTATGGAAATTCGAAACCTCCAAATACTACTTCACCATCAT
TGATGCCCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCCCAAGCCGA
TTGTGCCGTCTTAGTCATTGCCTCCCCAACTGGTGAATTCGAAGCTGGTATCGCCAAAAA
CGGTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGT
TGCTATCAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGACGAAAT
TGTCAAGGAAGTTTCATCTTTCATCAAAAAGATTGGTTACAATCCXXXXXXXXXX

*xtlkke*TTMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASEMG
KQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQAD
CAVLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIVAINKMDEKSTNYSQARYDEI
VKEVSSFIKKIGYN---

Frame A:
ixnfkerinnhg*rensh*hcrhwsrrcw*inhhwsfnlqmwwyr*esyrkv*kgsf*ng
*tilqirlgyg*ikg*t*kryhhrycfmeirnlqillhhh*cprsqrfhqeydywylpsr
lcrlshclpnw*irswyrqkrsns*trspclhfrcqtndrcyqqdg*kinqlltsplrrn
cqgsfifhqkdwlq---


Frame B:
*xtlkke*TTMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASEMG
KQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQAD
CAVLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIVAINKMDEKSTNYSQARYDEI
VKEVSSFIKKIGYN---


Frame C:
nxl*rknkqpwvkrkltltlsslvt*mlvnqpplvi*stnvvvsirelsksmkrkllkwv
nnpsntpglwin*rlnvkevspsillygnskppnttspslmpqvteissri*llvppkpi
vps*slppqlvnsklvspktvklvntlslltl*vsnk*sllstrwmknqptthkpvttkl
srkfhlsskrlvti---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO278 (VFO278Q) /CSM/VF/VFO2-D/VFO278Q.Seq.d/                       1154   0.0
VFN579 (VFN579Q) /CSM/VF/VFN5-D/VFN579Q.Seq.d/                       1154   0.0
VFN340 (VFN340Q) /CSM/VF/VFN3-B/VFN340Q.Seq.d/                       1154   0.0
VFN256 (VFN256Q) /CSM/VF/VFN2-C/VFN256Q.Seq.d/                       1154   0.0
VFN184 (VFN184Q) /CSM/VF/VFN1-D/VFN184Q.Seq.d/                       1154   0.0
VFN139 (VFN139Q) /CSM/VF/VFN1-B/VFN139Q.Seq.d/                       1154   0.0
VFM716 (VFM716Q) /CSM/VF/VFM7-A/VFM716Q.Seq.d/                       1154   0.0
VFM630 (VFM630Q) /CSM/VF/VFM6-B/VFM630Q.Seq.d/                       1154   0.0
VFM542 (VFM542Q) /CSM/VF/VFM5-B/VFM542Q.Seq.d/                       1154   0.0
VFM504 (VFM504Q) /CSM/VF/VFM5-A/VFM504Q.Seq.d/                       1154   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.  1080  0.0  1
X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.   886  0.0  1
AF016242|AF016242.1 Dictyostelium discoideum protein synthesis elongation factor 1-alpha (tef2) gene, partial cds.   682  0.0  2
AF157274|AF157274.1 Phascolomyces articulosus translation elongation factor 1-alpha (EF-1alpha) gene, partial cds.   105  2e-86  7
M29934|M29934.1 C.albicans elongation factor 1-alpha (TEF-1) gene, complete cds.   192  5e-86  4
CA754741|0   135  2e-85  5
AF157251|AF157251.1 Gilbertella persicaria translation elongation factor 1-alpha (EF-1alpha) gene, partial cds.   109  3e-85  6
AJ562756|AJ562756.1 Cryptosporidium parvum GSS, PAC clone pica_0011_b10, T7 end  sequence.   210  2e-84  4
CB909948|CB909948.1 USDA-FP_101691 Adult Alate Brown Citrus Aphid Toxoptera citricida cDNA clone WHWTC-24_A11 5', mRNA sequence.   220  3e-84  3
AL437199|AL437199.1 T7 end of clone BC0AA007H06 of library BC0AA from strain CBS 767 of Debaryomyces hansenii.   105  5e-83  6

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   361   9e-99
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   361   9e-99
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   361   9e-99
BC092884_1(BC092884|pid:none) Danio rerio zgc:110335, mRNA (cDNA...   322   4e-87
BC060907_1(BC060907|pid:none) Danio rerio zgc:73138, mRNA (cDNA ...   320   1e-86
BC075885_1(BC075885|pid:none) Danio rerio zgc:92085, mRNA (cDNA ...   320   2e-86
(P13549) RecName: Full=Elongation factor 1-alpha, somatic form; ...   320   2e-86
(Q90835) RecName: Full=Elongation factor 1-alpha 1;          Sho...   319   3e-86
AK167843_1(AK167843|pid:none) Mus musculus TIB-55 BB88 cDNA, RIK...   319   3e-86
AB174733_1(AB174733|pid:none) Macaca fascicularis brain cDNA clo...   319   3e-86

 psg: 0.84  gvh: 0.34  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for AFB241 is cyt

>AFB241F.Seq
ATAATNAACTTTAAAGAAAGAATAAACAACCATGGGTAAAGAGAAAACTCACATTAACAT
TGTCGTCATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAA
ATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTATGAAAAGGAAGCTTCTGAAATGGG
TAAACAATCCTTCAAATACGCCTGGGTTATGGATAAATTAAAGGCTGAACGTGAAAGAGG
TATCACCATCGATATTGCTTTATGGAAATTCGAAACCTCCAAATACTACTTCACCATCAT
TGATGCCCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCCCAAGCCGA
TTGTGCCGTCTTAGTCATTGCCTCCCCAACTGGTGAATTCGAAGCTGGTATCGCCAAAAA
CGGTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGT
TGCTATCAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGACGAAAT
TGTCAAGGAAGTTTCATCTTTCATCAAAAAGATTGGTTACAATCC----------