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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLF274
Library SL
(Link to library)
Clone ID SLF274
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12164-1
Original site URL
Representative seq. ID SLF274P
(Link to Original site)
Representative DNA sequence 
>SLF274 (SLF274Q) /CSM/SL/SLF2-D/SLF274Q.Seq.d/
AATCTCTGCTTTAATCTTACAATTCTGTTCTTCATGTTCAATTAATCTATGTTTACAAAT
TATGTCTTGTGCTCATTTAACTTTAAATCAATCATTGTCTAACTATTGTAAAGTCATTGA
TAATATTCTAAGATCTTTCTACTCAATCGATACCAATAAATCAATGGGAATTGGTGGTGG
TGAAGGTGATGAAAATAGTAATTCCACTGGTACAAACAATACAAATCTCATTAATAAATA
CTTGATTTTCCCAAATAATCATGXXXXXXXXXXATCATGAATATGAACTTTGGTTATCTT
GGATGTTGGTAATTAAATTAATTGAAAATCAATCTCAATCAAGTTTTAAAAATAAACATT
TAATTCAATCTATTTCAGATTTTAAAGATTCAATATCATCATATTTTGGAACAGCTGGTA
ATAAGATAACATCATTATTTTCAAATAAACAAAAGACAATAATGGTACAGACTCATTTAG
CAGCAAGAGCATGTTTATTATATTTACAGAGAATATTGGTTCAAAGAAAAGAAAAAGATG
GTGAAAAAACAATCAAAGATGTTCAATTATTAAATCAAATCACTGATAGTCTCGTTGATT
TGAAAAAGAAATCAACCAATAATATCTATTCACCATATCAAGATTTCTTTAATGAATCAG
AAAACTTTTTATCACCATTGGTAGAATTTGAATTCTTTTCAAACTCTGTCATTAGAACTC
TTGTACCATTTGCAAATTATATTTTAAATATTTTATCTTATGAATAAAAAAAAAATAATA
ATAACAATAAATAATAATAATAAAATAATAATAA
sequence update 1999. 2.12
Translated Amino Acid sequence 
ISALILQFCSSCSINLCLQIMSCAHLTLNQSLSNYCKVIDNILRSFYSIDTNKSMGIGGG
EGDENSNSTGTNNTNLINKYLIFPNNH---

---HEYELWLSWMLVIKLIENQSQSSFKNKHLIQSISDFKDSISSYFGTAGNKITSLFSN
KQKTIMVQTHLAARACLLYLQRILVQRKEKDGEKTIKDVQLLNQITDSLVDLKKKSTNNI
YSPYQDFFNESENFLSPLVEFEFFSNSVIRTLVPFANYILNILSYE*kknnnnnk****n
nn
Translated Amino Acid sequence (All Frames) 
Frame A:
nlcfnltilffmfn*smftnyvlcsfnfksiiv*ll*sh**yskifllnryq*ingnwww
*r**k**fhwykqyksh**ildfpk*s---

---imnmnfgylgcw*ln*lkinlnqvlkini*fnlfqilkiqyhhileqlvir*hhyfq
inkrq*wyrli*qqehvyyiyreywfkekkkmvkkqskmfny*ikslivsli*krnqpii
sihhikislmnqktfyhhw*nlnsfqtlslellyhlqiif*ifylmnkkkiiitinnnnk
iii

Frame B:
ISALILQFCSSCSINLCLQIMSCAHLTLNQSLSNYCKVIDNILRSFYSIDTNKSMGIGGG
EGDENSNSTGTNNTNLINKYLIFPNNH---

---s*i*tlvildvgn*in*ksisikf*k*tfnsiyfrf*rfniiifwnsw**dniiifk
*tkdnngtdsfssksmfiiftenigskkrkrw*knnqrcsiiksnh**sr*fekeinq*y
lftisrfl**irklfitigri*ilfklch*nscticklyfkyfil*ikkk***q*iiiik
***

Frame C:
sll*synsvlhvqliyvyklclvli*l*inhcltivksliif*dlstqsipinqwelvvv
kvmkiviplvqtiqislint*fsqiim---

---HEYELWLSWMLVIKLIENQSQSSFKNKHLIQSISDFKDSISSYFGTAGNKITSLFSN
KQKTIMVQTHLAARACLLYLQRILVQRKEKDGEKTIKDVQLLNQITDSLVDLKKKSTNNI
YSPYQDFFNESENFLSPLVEFEFFSNSVIRTLVPFANYILNILSYE*kknnnnnk****n
nn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLF274 (SLF274Q) /CSM/SL/SLF2-D/SLF274Q.Seq.d/                        975   0.0
VHN171 (VHN171Q) /CSM/VH/VHN1-C/VHN171Q.Seq.d/                        482   e-135
VHI203 (VHI203Q) /CSM/VH/VHI2-A/VHI203Q.Seq.d/                        476   e-133
VSI108 (VSI108Q) /CSM/VS/VSI1-A/VSI108Q.Seq.d/                         36   0.81
VSB554 (VSB554Q) /CSM/VS/VSB5-C/VSB554Q.Seq.d/                         36   0.81
VHL171 (VHL171Q) /CSM/VH/VHL1-C/VHL171Q.Seq.d/                         36   0.81
VHA777 (VHA777Q) /CSM/VH/VHA7-D/VHA777Q.Seq.d/                         36   0.81
VFI490 (VFI490Q) /CSM/VF/VFI4-D/VFI490Q.Seq.d/                         36   0.81
VFI308 (VFI308Q) /CSM/VF/VFI3-A/VFI308Q.Seq.d/                         36   0.81
VFD259 (VFD259Q) /CSM/VF/VFD2-C/VFD259Q.Seq.d/                         36   0.81
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU052889) Dictyostelium discoideum slug cDNA, clone SLF274.      494   0.0    2
(BJ441546) Dictyostelium discoideum cDNA clone:ddv47e02, 3' ...   476   0.0    3
(BJ444862) Dictyostelium discoideum cDNA clone:ddv57m17, 3' ...   482   0.0    3
(AU061658) Dictyostelium discoideum slug cDNA, clone SLF274.      519   e-143  1
(AC116977) Dictyostelium discoideum chromosome 2 map 5515173...    36   5e-04  14
(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...    36   0.001  14
(AC083825) Homo sapiens chromosome 3 clone RP11-208N8, WORKI...    34   0.009  10
(AC212313) Solanum lycopersicum chromosome 07 clone C07SLm00...    42   0.017  7
(AX346417) Sequence 1488 from Patent WO0200928.                    44   0.031  2
(AC210366) Solanum lycopersicum chromosome 7 clone C07HBa030...    42   0.031  6
dna update 2009. 5. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF325215_2(AF325215|pid:none) Lymphocytic choriomeningitis virus...    39   0.20
AE014187_324(AE014187|pid:none) Plasmodium falciparum 3D7 chromo...    37   0.75
EU480452_2(EU480452|pid:none) Lymphocytic choriomeningitis virus...    36   1.3
AF325214_2(AF325214|pid:none) Lymphocytic choriomeningitis virus...    36   1.7
(P09992) RecName: Full=Nucleoprotein; AltName: Full=Nucleocapsid...    35   2.2
Y16308_1(Y16308|pid:none) Lymphocytic choriomeningitis virus gen...    35   3.7
AE001362_187(AE001362|pid:none) Plasmodium falciparum 3D7 chromo...    35   3.7
CP000764_1792(CP000764|pid:none) Bacillus cereus subsp. cytotoxi...    34   4.8
AE015450_332(AE015450|pid:none) Mycoplasma gallisepticum strain ...    34   4.8
DQ837165_69(DQ837165|pid:none) Ecotropis obliqua NPV strain A1, ...    34   4.8
protein update 2009. 6. 3
PSORT 

 psg: 0.90  gvh: 0.55  alm: 0.36  top: 0.57  tms: 0.00  mit: 0.36  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: nuclear
	 28.0 %: cytoplasmic
	 28.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall

>> prediction for SLF274 is nuc
5' end seq. ID SLF274F
5' end seq. 
>SLF274F.Seq
AATCTCTGCTTTAATCTTACAATTCTGTTCTTCATGTTCAATTAATCTATGTTTACAAAT
TATGTCTTGTGCTCATTTAACTTTAAATCAATCATTGTCTAACTATTGTAAAGTCATTGA
TAATATTCTAAGATCTTTCTACTCAATCGATACCAATAAATCAATGGGAATTGGTGGTGG
TGAAGGTGATGAAAATAGTAATTCCACTGGTACAAACAATACAAATCTCATTAATAAATA
CTTGATTTTCCCAAATAATCATG----------
Length of 5' end seq. 263
3' end seq. ID SLF274Z
3' end seq. 
>SLF274Z.Seq
----------ATCATGAATATGAACTTTGGTTATCTTGGATGTTGGTAATTAAATTAATT
GAAAATCAATCTCAATCAAGTTTTAAAAATAAACATTTAATTCAATCTATTTCAGATTTT
AAAGATTCAATATCATCATATTTTGGAACAGCTGGTAATAAGATAACATCATTATTTTCA
AATAAACAAAAGACAATAATGGTACAGACTCATTTAGCAGCAAGAGCATGTTTATTATAT
TTACAGAGAATATTGGTTCAAAGAAAAGAAAAAGATGGTGAAAAAACAATCAAAGATGTT
CAATTATTAAATCAAATCACTGATAGTCTCGTTGATTTGAAAAAGAAATCAACCAATAAT
ATCTATTCACCATATCAAGATTTCTTTAATGAATCAGAAAACTTTTTATCACCATTGGTA
GAATTTGAATTCTTTTCAAACTCTGTCATTAGAACTCTTGTACCATTTGCAAATTATATT
TTAAATATTTTATCTTATGAATAAAAAAAAAATAATAATAACAATAAATAATAATAATAA
AATAATAATAA
Length of 3' end seq. 541
Connected seq. ID SLF274P
Connected seq. 
>SLF274P.Seq
AATCTCTGCTTTAATCTTACAATTCTGTTCTTCATGTTCAATTAATCTATGTTTACAAAT
TATGTCTTGTGCTCATTTAACTTTAAATCAATCATTGTCTAACTATTGTAAAGTCATTGA
TAATATTCTAAGATCTTTCTACTCAATCGATACCAATAAATCAATGGGAATTGGTGGTGG
TGAAGGTGATGAAAATAGTAATTCCACTGGTACAAACAATACAAATCTCATTAATAAATA
CTTGATTTTCCCAAATAATCATG----------ATCATGAATATGAACTTTGGTTATCTT
GGATGTTGGTAATTAAATTAATTGAAAATCAATCTCAATCAAGTTTTAAAAATAAACATT
TAATTCAATCTATTTCAGATTTTAAAGATTCAATATCATCATATTTTGGAACAGCTGGTA
ATAAGATAACATCATTATTTTCAAATAAACAAAAGACAATAATGGTACAGACTCATTTAG
CAGCAAGAGCATGTTTATTATATTTACAGAGAATATTGGTTCAAAGAAAAGAAAAAGATG
GTGAAAAAACAATCAAAGATGTTCAATTATTAAATCAAATCACTGATAGTCTCGTTGATT
TGAAAAAGAAATCAACCAATAATATCTATTCACCATATCAAGATTTCTTTAATGAATCAG
AAAACTTTTTATCACCATTGGTAGAATTTGAATTCTTTTCAAACTCTGTCATTAGAACTC
TTGTACCATTTGCAAATTATATTTTAAATATTTTATCTTATGAATAAAAAAAAAATAATA
ATAACAATAAATAATAATAATAAAATAATAATAA
Length of connected seq. 804
Full length Seq ID -
Full length Seq. -
Length of full length seq. -