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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK413
Library AF
(Link to library)
Clone ID AFK413
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12894-1
Original site URL
Representative seq. ID AFK413P
(Link to Original site)
Representative DNA sequence 
>AFK413 (AFK413Q) /CSM/AF/AFK4-A/AFK413Q.Seq.d/
TATTTTTTTTTTTACAATAATGAAATGAAAATTTTAATATTATCATTTTTCTTAATTTTA
GGAATAAATTTAGTATTTTGTCAAAGTTCCATTTNTTTAAGTGCCACTATTTATGATCAA
AGTCCTGCACGTAATCCTGATTTCGAAATACCAAATGCTCCAAATGCTGTAGTTAANAAT
ATGGTTCTAAGTACTTTAGGTGCTGATAAAACTCCAACCTATTGTTGNGGNGATAATGGN
GTTTATTATAATGGTCAATTTGATATTCATAGCCAATCCACATTTGATTCCTGGTTCCAT
GAAATTGAAGGTATCAGTTATGCCGTTCCATACGAATTAGTTTTAATTGAATCGAAAGAC
GAACCAGGAATTTATGTATATACCTCTAGTTCTTTCTTCCCAATTGATGGTAAAGGTTTT
GATAATAAAACATTATACCCAGATGAAATCACCTATAATGGTCACAACTACCATTTTTGT
ATGAAAATTCATAGTAGTTTTACCTATCATGXXXXXXXXXXAAAGAATTGTAGTGATGGT
GATATTTGTTCAACTGATGGTTGTGATATTGGTTTTGGATGTATTAATTTACCTATAAAC
TGTGATGATGGAAATTATTGTACTAAAGATTATTGTGATCCAACATATGGATGTTTACAT
ATCGAAACTGCAAATTGCACTGAATGTTTAGAAACAGGTTGTATAACTGTTCTCCCATGT
TTCCCAGTAACATGTGACAGTTTAAATAGTAGCCACTGTGTTGCTAGTGCTATGAATTGC
TCAGATAACGATCCATGTACATCAACAGAATGCGTTAATGGAGAATGTACATATGAGTGG
ATTTGTGGATCAGAAGATTCATCATCTGGTGTTGTAGATTCATCATCTGGTGTTGTAGAT
TCATCATCTGATGTTATTGACTCTTCAGATATTATTATTGATTCTTCATCCGATATCCCA
ATACCAACACTATCACCTTCCCCTCAACCATCACGTTTCCCAACTGATACACCAACTAAT
ACACCAATGCCACCAACCCGTCCTCCAACTCCAACTGAAGATCCAAAGATCTATGAAGAT
CCAGAAGATTTAGATAAAGATTGTTTACATTGTAAAGATTTAGGTTGCATACTAACTGGT
AAAAGTTGTGGTTACTTAAAAAATAAAAATACGAAAAATCAAATTGTAAAGAGAATTGTT
GTTCACAACTCCAACTGTTTTTAATTTTCAATTNATTTAAAAAATAAAAAATAATAATAA
AATAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
YFFFYNNEMKILILSFFLILGINLVFCQSSIXLSATIYDQSPARNPDFEIPNAPNAVVXN
MVLSTLGADKTPTYCXGDNGVYYNGQFDIHSQSTFDSWFHEIEGISYAVPYELVLIESKD
EPGIYVYTSSSFFPIDGKGFDNKTLYPDEITYNGHNYHFCMKIHSSFTYH---

---KNCSDGDICSTDGCDIGFGCINLPINCDDGNYCTKDYCDPTYGCLHIETANCTECLE
TGCITVLPCFPVTCDSLNSSHCVASAMNCSDNDPCTSTECVNGECTYEWICGSEDSSSGV
VDSSSGVVDSSSDVIDSSDIIIDSSSDIPIPTLSPSPQPSRFPTDTPTNTPMPPTRPPTP
TEDPKIYEDPEDLDKDCLHCKDLGCILTGKSCGYLKNKNTKNQIVKRIVVHNSNCF*fsi
xlknkk***nk
Translated Amino Acid sequence (All Frames) 
Frame A:
YFFFYNNEMKILILSFFLILGINLVFCQSSIXLSATIYDQSPARNPDFEIPNAPNAVVXN
MVLSTLGADKTPTYCXGDNGVYYNGQFDIHSQSTFDSWFHEIEGISYAVPYELVLIESKD
EPGIYVYTSSSFFPIDGKGFDNKTLYPDEITYNGHNYHFCMKIHSSFTYH---

---kel**w*ylfn*wl*ywfwmy*ftykl**wklly*rll*sniwmftyrncklh*mfr
nrlyncspmfpsnm*qfk**plcc*cyellr*rsmyinrmr*wrmyi*vdlwirrfiiwc
crfiiwccrfii*cy*lfryyy*ffirypntntitfpstitfpn*ytn*ytnatnpssns
n*rskdl*rsrrfr*rlftl*rfrlhtnw*klwllkk*kyeksnckenccsqlqlflifn
xfkk*kiiik*

Frame B:
iffftimk*kf*yyhfs*f*e*i*yfvkvpfx*vplfmikvlhviliskyqmlqml*lxi
wf*vl*vliklqpivxximxfiimvnlifianphlipgsmklkvsvmpfhtn*f*lnrkt
nqefmyiplvlssqlmvkvliikhytqmkspimvtttifv*kfivvlpim---

---KNCSDGDICSTDGCDIGFGCINLPINCDDGNYCTKDYCDPTYGCLHIETANCTECLE
TGCITVLPCFPVTCDSLNSSHCVASAMNCSDNDPCTSTECVNGECTYEWICGSEDSSSGV
VDSSSGVVDSSSDVIDSSDIIIDSSSDIPIPTLSPSPQPSRFPTDTPTNTPMPPTRPPTP
TEDPKIYEDPEDLDKDCLHCKDLGCILTGKSCGYLKNKNTKNQIVKRIVVHNSNCF*fsi
xlknkk***nk

Frame C:
ffflq**nenfniiiflnfrnkfsilskfhxfkchyl*sksct*s*frntkcskccs*xy
gskyfrc**nsnlllxx*wxll*wsi*ys*pihi*flvp*n*ryqlcrsirisfn*ierr
trnlciyl*fflpn*w*rf***niipr*nhl*wsqlpflyens**fyls---

---rivvmvifvqlmvvilvldvliyl*tvmmeiivlkiiviqhmdvyisklqialnv*k
qvv*lfshvsq*hvtv*ivatvllvl*iaqitihvhqqnalmenvhmsgfvdqkihhlvl
*ihhlvl*ihhlmlltlqilllilhpisqyqhyhlplnhhvsqlihqlihqchqpvlqlq
lkiqrsmkiqki*ikivyivki*vay*lvkvvvt*kikirkikl*rellfttptvfnfqx
i*kiknnnki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK413 (AFK413Q) /CSM/AF/AFK4-A/AFK413Q.Seq.d/                       2179   0.0
CFI858 (CFI858Q) /CSM/CF/CFI8-C/CFI858Q.Seq.d/                       1253   0.0
AFM178 (AFM178Q) /CSM/AF/AFM1-D/AFM178Q.Seq.d/                       1253   0.0
AFL748 (AFL748Q) /CSM/AF/AFL7-B/AFL748Q.Seq.d/                       1253   0.0
CFA542 (CFA542Q) /CSM/CF/CFA5-B/CFA542Q.Seq.d/                       1249   0.0
CFH396 (CFH396Q) /CSM/CF/CFH3-D/CFH396Q.Seq.d/                       1247   0.0
SFE489 (SFE489Q) /CSM/SF/SFE4-D/SFE489Q.Seq.d/                       1245   0.0
CFH122 (CFH122Q) /CSM/CF/CFH1-A/CFH122Q.Seq.d/                       1245   0.0
SFJ670 (SFJ670Q) /CSM/SF/SFJ6-C/SFJ670Q.Seq.d/                       1243   0.0
AFK842 (AFK842Q) /CSM/AF/AFK8-B/AFK842Q.Seq.d/                       1241   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    36   5e-05  16
AE017338|AE017338.1 Mycoplasma mobile 163K section 2 of 3 of the complete genome.    34   4e-04  14
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    38   0.005  19
BX000480|BX000480.6 Zebrafish DNA sequence from clone DKEY-14C5 in linkage group 17.    46   0.006  6
L12018|L12018.2 Caenorhabditis elegans cosmid F22B7, complete sequence.    32   0.027  7
AC116551|AC116551.2 Dictyostelium discoideum chromosome 2 map complement(581327-427576) strain AX4, complete sequence.    32   0.031  13
BX842643|BX842643.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 2/4.    36   0.070  15
AL109815|AL109815.2 Plasmodium falciparum DNA *** SEQUENCING IN PROGRESS *** from  MAL13P10.    50   0.088  1
AA550688|AA550688.1 1925m3 gmbPfHB3.1, G. Roman Reddy Plasmodium falciparum genomic clone 1925m, DNA sequence.    50   0.088  1
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           50   0.088  1
dna update 2004. 7.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q1ZXC5) RecName: Full=Protein psiC; Flags: Precursor;                313   6e-84
(Q54FD8) RecName: Full=Protein psiD; Flags: Precursor;                173   1e-41
(Q54C31) RecName: Full=Protein psiR; Flags: Precursor;                144   5e-33
(Q54C32) RecName: Full=Protein psiQ; Flags: Precursor;                143   1e-32
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                112   3e-23
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                100   2e-19
AC116984_103(AC116984|pid:none) Dictyostelium discoideum chromos...    85   6e-15
AC117072_61(AC117072|pid:none) Dictyostelium discoideum chromoso...    83   2e-14
(P11976) RecName: Full=Prestalk protein; AltName: Full=Extracell...    82   5e-14
A26838(A26838)prestalk protein precursor - slime mold (Dictyoste...    82   5e-14
protein update 2009. 4.24
PSORT 

 psg: 0.99  gvh: 0.68  alm: 0.38  top: 0.33  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 24.0 %: cytoplasmic
	 12.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: endoplasmic reticulum

>> prediction for AFK413 is exc
5' end seq. ID AFK413F
5' end seq. 
>AFK413F.Seq
TATTTTTTTTTTTACAATAATGAAATGAAAATTTTAATATTATCATTTTTCTTAATTTTA
GGAATAAATTTAGTATTTTGTCAAAGTTCCATTTNTTTAAGTGCCACTATTTATGATCAA
AGTCCTGCACGTAATCCTGATTTCGAAATACCAAATGCTCCAAATGCTGTAGTTAANAAT
ATGGTTCTAAGTACTTTAGGTGCTGATAAAACTCCAACCTATTGTTGNGGNGATAATGGN
GTTTATTATAATGGTCAATTTGATATTCATAGCCAATCCACATTTGATTCCTGGTTCCAT
GAAATTGAAGGTATCAGTTATGCCGTTCCATACGAATTAGTTTTAATTGAATCGAAAGAC
GAACCAGGAATTTATGTATATACCTCTAGTTCTTTCTTCCCAATTGATGGTAAAGGTTTT
GATAATAAAACATTATACCCAGATGAAATCACCTATAATGGTCACAACTACCATTTTTGT
ATGAAAATTCATAGTAGTTTTACCTATCATG----------
Length of 5' end seq. 511
3' end seq. ID AFK413Z
3' end seq. 
>AFK413Z.Seq
----------AAAGAATTGTAGTGATGGTGATATTTGTTCAACTGATGGTTGTGATATTG
GTTTTGGATGTATTAATTTACCTATAAACTGTGATGATGGAAATTATTGTACTAAAGATT
ATTGTGATCCAACATATGGATGTTTACATATCGAAACTGCAAATTGCACTGAATGTTTAG
AAACAGGTTGTATAACTGTTCTCCCATGTTTCCCAGTAACATGTGACAGTTTAAATAGTA
GCCACTGTGTTGCTAGTGCTATGAATTGCTCAGATAACGATCCATGTACATCAACAGAAT
GCGTTAATGGAGAATGTACATATGAGTGGATTTGTGGATCAGAAGATTCATCATCTGGTG
TTGTAGATTCATCATCTGGTGTTGTAGATTCATCATCTGATGTTATTGACTCTTCAGATA
TTATTATTGATTCTTCATCCGATATCCCAATACCAACACTATCACCTTCCCCTCAACCAT
CACGTTTCCCAACTGATACACCAACTAATACACCAATGCCACCAACCCGTCCTCCAACTC
CAACTGAAGATCCAAAGATCTATGAAGATCCAGAAGATTTAGATAAAGATTGTTTACATT
GTAAAGATTTAGGTTGCATACTAACTGGTAAAAGTTGTGGTTACTTAAAAAATAAAAATA
CGAAAAATCAAATTGTAAAGAGAATTGTTGTTCACAACTCCAACTGTTTTTAATTTTCAA
TTNATTTAAAAAATAAAAAATAATAATAAAATAAA
Length of 3' end seq. 745
Connected seq. ID AFK413P
Connected seq. 
>AFK413P.Seq
TATTTTTTTTTTTACAATAATGAAATGAAAATTTTAATATTATCATTTTTCTTAATTTTA
GGAATAAATTTAGTATTTTGTCAAAGTTCCATTTNTTTAAGTGCCACTATTTATGATCAA
AGTCCTGCACGTAATCCTGATTTCGAAATACCAAATGCTCCAAATGCTGTAGTTAANAAT
ATGGTTCTAAGTACTTTAGGTGCTGATAAAACTCCAACCTATTGTTGNGGNGATAATGGN
GTTTATTATAATGGTCAATTTGATATTCATAGCCAATCCACATTTGATTCCTGGTTCCAT
GAAATTGAAGGTATCAGTTATGCCGTTCCATACGAATTAGTTTTAATTGAATCGAAAGAC
GAACCAGGAATTTATGTATATACCTCTAGTTCTTTCTTCCCAATTGATGGTAAAGGTTTT
GATAATAAAACATTATACCCAGATGAAATCACCTATAATGGTCACAACTACCATTTTTGT
ATGAAAATTCATAGTAGTTTTACCTATCATG----------AAAGAATTGTAGTGATGGT
GATATTTGTTCAACTGATGGTTGTGATATTGGTTTTGGATGTATTAATTTACCTATAAAC
TGTGATGATGGAAATTATTGTACTAAAGATTATTGTGATCCAACATATGGATGTTTACAT
ATCGAAACTGCAAATTGCACTGAATGTTTAGAAACAGGTTGTATAACTGTTCTCCCATGT
TTCCCAGTAACATGTGACAGTTTAAATAGTAGCCACTGTGTTGCTAGTGCTATGAATTGC
TCAGATAACGATCCATGTACATCAACAGAATGCGTTAATGGAGAATGTACATATGAGTGG
ATTTGTGGATCAGAAGATTCATCATCTGGTGTTGTAGATTCATCATCTGGTGTTGTAGAT
TCATCATCTGATGTTATTGACTCTTCAGATATTATTATTGATTCTTCATCCGATATCCCA
ATACCAACACTATCACCTTCCCCTCAACCATCACGTTTCCCAACTGATACACCAACTAAT
ACACCAATGCCACCAACCCGTCCTCCAACTCCAACTGAAGATCCAAAGATCTATGAAGAT
CCAGAAGATTTAGATAAAGATTGTTTACATTGTAAAGATTTAGGTTGCATACTAACTGGT
AAAAGTTGTGGTTACTTAAAAAATAAAAATACGAAAAATCAAATTGTAAAGAGAATTGTT
GTTCACAACTCCAACTGTTTTTAATTTTCAATTNATTTAAAAAATAAAAAATAATAATAA
AATAAA
Length of connected seq. 1256
Full length Seq ID -
Full length Seq. -
Length of full length seq. -