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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFB336
Library AF
(Link to library)
Clone ID AFB336
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16173-1
Original site URL
Representative seq. ID AFB336P
(Link to Original site)
Representative DNA sequence 
>AFB336 (AFB336Q) /CSM/AF/AFB3-B/AFB336Q.Seq.d/
CTGTTGGCCTACTGGNAGGATAGTCTCCACCAAACGATATTTATATACATCCAATATATT
TTTATATATTGAATTAAAATTTTAAAAAAAGAATGTCAAACAAGGTATTAATGATTGATG
GTGATCGTATCAGTGGTAATGAAGTTAGAGCACAAAATGTTTTAGCAGTTACAGCAATTG
CAAATATAGTTAAAACATCATTTGGACCAATTGGTTTAGATAAAATGTTAATTGATAATA
TTGGATCAATTGTTGTTACCAATGATGGTGCAACCATTTTACAAAAGATTGATATAGAAC
ATCCAGCAGCAAAGATTTTAGTACAATTATCAGAATTGCAAGATCAAGAAGTTGGTGATG
GTACAACTACAGTTGTAATTTTAGCAGCAGAATTATTGAAACGTGCCAATGAATTGGTAG
CACGTAAAGTACATCCAACAGTTATTATTAGTGGTTTTAGATTAGCATGTACAGAAGCAN
TCAAATXXXXXXXXXXCTGATTTAGTTTCAAAATTAAAAGCCTATCATCATGCCGCTCAA
ACTGATCCATCAAAGAAATCTTACGCATACTCTGGTCTCGATTTATTCAATAACAAAGTT
AGAAATAATTTAGAGGCTGGTGTTTTAGAACCTGCCATTGCCAAAATCAAATGTATCAAA
TTTGCNNNTNAAAGCGCTATCACCATCCNTCGTATCGATGAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
vgllxg*sppndiyihpiyfyiln*NFKKRMSNKVLMIDGDRISGNEVRAQNVLAVTAIA
NIVKTSFGPIGLDKMLIDNIGSIVVTNDGATILQKIDIEHPAAKILVQLSELQDQEVGDG
TTTVVILAAELLKRANELVARKVHPTVIISGFRLACTEAXK---

---DLVSKLKAYHHAAQTDPSKKSYAYSGLDLFNNKVRNNLEAGVLEPAIAKIKCIKFAX
XSAITIXRIDD
Translated Amino Acid sequence (All Frames) 
Frame A:
llaywxdslhqtifiyiqyifiy*ikilkkecqtry**lmvivsvvmklehkmf*qlqql
qi*lkhhldqlv*ikc*liildqlllpmmvqpfykrli*niqqqrf*ynyqnckikklvm
vqlql*f*qqny*nvpmnw*hvkyiqqlllvvld*hvqkxsn---

---li*fqn*kpiimplklihqrnlthtlvsiysitkleii*rlvf*nlplpksnvsnlx
xkalspsxvsm

Frame B:
cwptgrivstkrylytsniflyielkf*kknvkqgind*w*syqw**s*stkcfssysnc
kys*niiwtnwfr*nvn**ywinccyq*wcnhftkd*yrtssskdfstiiriarsrsw*w
ynyscnfssriietcq*igst*stsnsyy*wf*ismyrsxq---

---*fsfkikslsscrsn*sikeilrilwsrfiq*qs*k*frgwcfrtchcqnqmyqicx
xkryhhpsyr*

Frame C:
vgllxg*sppndiyihpiyfyiln*NFKKRMSNKVLMIDGDRISGNEVRAQNVLAVTAIA
NIVKTSFGPIGLDKMLIDNIGSIVVTNDGATILQKIDIEHPAAKILVQLSELQDQEVGDG
TTTVVILAAELLKRANELVARKVHPTVIISGFRLACTEAXK---

---DLVSKLKAYHHAAQTDPSKKSYAYSGLDLFNNKVRNNLEAGVLEPAIAKIKCIKFAX
XSAITIXRIDD
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB336 (AFB336Q) /CSM/AF/AFB3-B/AFB336Q.Seq.d/                       1249   0.0
VFI330 (VFI330Q) /CSM/VF/VFI3-B/VFI330Q.Seq.d/                        880   0.0
VFF578 (VFF578Q) /CSM/VF/VFF5-D/VFF578Q.Seq.d/                        880   0.0
AHI886 (AHI886Q) /CSM/AH/AHI8-D/AHI886Q.Seq.d/                        874   0.0
FC-AG03 (FC-AG03Q) /CSM/FC/FC-AG/FC-AG03Q.Seq.d/                      860   0.0
FC-116 (FC-116Q) /CSM/CG/CG-FC/FC-116Q.Seq.d/                         860   0.0
AFD439 (AFD439Q) /CSM/AF/AFD4-B/AFD439Q.Seq.d/                        860   0.0
VFD589 (VFD589Q) /CSM/VF/VFD5-D/VFD589Q.Seq.d/                        858   0.0
CFH434 (CFH434Q) /CSM/CF/CFH4-B/CFH434Q.Seq.d/                        852   0.0
AFG366 (AFG366Q) /CSM/AF/AFG3-C/AFG366Q.Seq.d/                        831   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AB008158|AB008158.1 Dictyostelium discoideum DdTcp-1 mRNA for t-complex polypeptide 1 homologue, complete cds.   779  0.0  3
AZ530054|AZ530054.1 ENTDW96TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    66  3e-17  3
AZ526360|AZ526360.1 254PbF04 Pb MBN #21 Plasmodium berghei genomic 3', DNA sequence.    70  4e-15  3
AZ522254|AZ522254.1 204PbE04 Pb MBN #21 Plasmodium berghei genomic 3', DNA sequence.    68  3e-13  2
BF298951|BF298951.1 068PbG10 Pb cDNA #20, Charles Yowell and Jane Carlton Plasmodium berghei cDNA 5', mRNA sequence.    68  3e-13  2
BF299139|BF299139.1 069PbF12 Pb cDNA #20, Charles Yowell and Jane Carlton Plasmodium berghei cDNA 5', mRNA sequence.    68  4e-13  2
AL437502|AL437502.1 T3 end of clone BC0AA009G02 of library BC0AA from strain CBS 767 of Debaryomyces hansenii.    48  3e-11  4
AA550135|AA550135.1 1237m3 gmbPfHB3.1, G. Roman Reddy Plasmodium falciparum genomic clone 1237m, DNA sequence.    60  3e-06  2
BP134604|BP134604.1 Nicotiana tabacum cDNA, clone: BY7020, primer: M13 forward, sequence 1.    44  1e-05  2
AX488977|AX488977.1 Sequence 6277 from Patent WO02053728.         42  3e-05  3
dna update 2003. 7.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB008158_1(AB008158|pid:none) Dictyostelium discoideum DdTcp-1 m...   247   3e-64
AF442545_1(AF442545|pid:none) Physarum polycephalum CCT chaperon...   188   1e-46
AC152752_1(AC152752|pid:none) Medicago truncatula clone mth2-89c...   180   4e-44
(P41988) RecName: Full=T-complex protein 1 subunit alpha;       ...   180   4e-44
AP000383_4(AP000383|pid:none) Arabidopsis thaliana genomic DNA, ...   179   7e-44
BT002052_1(BT002052|pid:none) Arabidopsis thaliana t-complex pol...   179   7e-44
AB194475_1(AB194475|pid:none) Delia antiqua DaTCP-1 mRNA for T-c...   178   2e-43
A45915_1(A45915|pid:none) Sequence 54 from Patent WO9520654.          177   3e-43
AK167529_1(AK167529|pid:none) Mus musculus 14 days pregnant adul...   177   3e-43
AK166828_1(AK166828|pid:none) Mus musculus blastocyst blastocyst...   177   3e-43
protein update 2009. 3.27
PSORT 

 psg: 0.57  gvh: 0.40  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: cytoplasmic
	 20.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for AFB336 is cyt
5' end seq. ID AFB336F
5' end seq. 
>AFB336F.Seq
CTGTTGGCCTACTGGNAGGATAGTCTCCACCAAACGATATTTATATACATCCAATATATT
TTTATATATTGAATTAAAATTTTAAAAAAAGAATGTCAAACAAGGTATTAATGATTGATG
GTGATCGTATCAGTGGTAATGAAGTTAGAGCACAAAATGTTTTAGCAGTTACAGCAATTG
CAAATATAGTTAAAACATCATTTGGACCAATTGGTTTAGATAAAATGTTAATTGATAATA
TTGGATCAATTGTTGTTACCAATGATGGTGCAACCATTTTACAAAAGATTGATATAGAAC
ATCCAGCAGCAAAGATTTTAGTACAATTATCAGAATTGCAAGATCAAGAAGTTGGTGATG
GTACAACTACAGTTGTAATTTTAGCAGCAGAATTATTGAAACGTGCCAATGAATTGGTAG
CACGTAAAGTACATCCAACAGTTATTATTAGTGGTTTTAGATTAGCATGTACAGAAGCAN
TCAAAT----------
Length of 5' end seq. 486
3' end seq. ID AFB336Z
3' end seq. 
>AFB336Z.Seq
----------CTGATTTAGTTTCAAAATTAAAAGCCTATCATCATGCCGCTCAAACTGAT
CCATCAAAGAAATCTTACGCATACTCTGGTCTCGATTTATTCAATAACAAAGTTAGAAAT
AATTTAGAGGCTGGTGTTTTAGAACCTGCCATTGCCAAAATCAAATGTATCAAATTTGCN
NNTNAAAGCGCTATCACCATCCNTCGTATCGATGAT
Length of 3' end seq. 206
Connected seq. ID AFB336P
Connected seq. 
>AFB336P.Seq
CTGTTGGCCTACTGGNAGGATAGTCTCCACCAAACGATATTTATATACATCCAATATATT
TTTATATATTGAATTAAAATTTTAAAAAAAGAATGTCAAACAAGGTATTAATGATTGATG
GTGATCGTATCAGTGGTAATGAAGTTAGAGCACAAAATGTTTTAGCAGTTACAGCAATTG
CAAATATAGTTAAAACATCATTTGGACCAATTGGTTTAGATAAAATGTTAATTGATAATA
TTGGATCAATTGTTGTTACCAATGATGGTGCAACCATTTTACAAAAGATTGATATAGAAC
ATCCAGCAGCAAAGATTTTAGTACAATTATCAGAATTGCAAGATCAAGAAGTTGGTGATG
GTACAACTACAGTTGTAATTTTAGCAGCAGAATTATTGAAACGTGCCAATGAATTGGTAG
CACGTAAAGTACATCCAACAGTTATTATTAGTGGTTTTAGATTAGCATGTACAGAAGCAN
TCAAAT----------CTGATTTAGTTTCAAAATTAAAAGCCTATCATCATGCCGCTCAA
ACTGATCCATCAAAGAAATCTTACGCATACTCTGGTCTCGATTTATTCAATAACAAAGTT
AGAAATAATTTAGAGGCTGGTGTTTTAGAACCTGCCATTGCCAAAATCAAATGTATCAAA
TTTGCNNNTNAAAGCGCTATCACCATCCNTCGTATCGATGAT
Length of connected seq. 692
Full length Seq ID -
Full length Seq. -
Length of full length seq. -