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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFL324
Library AF
(Link to library)
Clone ID AFL324
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U02971-1
Original site URL
Representative seq. ID AFL324P
(Link to Original site)
Representative DNA sequence 
>AFL324 (AFL324Q) /CSM/AF/AFL3-A/AFL324Q.Seq.d/
AAACAATAATAAACACACATGTCAAGAATATATGAACAAATAGAAAAAGATGTTGAAAAA
ATAATTGGTCAATATGATGGAGATAAAAATGGAGAAGTAACTATAAATGAAGCTATTGAA
TTTTTTAAAAGGATGGGATCAAAATATCCAGAGAAATGTGCAATTGTATTATTTAAAATG
TATGATCTAGATAATGAAGGAAAAATTTCATATGATGAAATTCAGGAAGAAATATTTAAA
AGATATCAAGATAAAGTAAGAGAGGATCAAATAAAACAATATTTTCAAGATGATATTGAA
GCATTTCTATTGAGATATGATAAAAATAGAGATAATAGAATTGATTTTAAAGAACTTGAA
CAATGTTTTGAAAGTATTGGTTCAGATCATCCAAAAGAAAATGCTAATCATATTTTCACT
GAAATTGATAAAAACAGAGATGGATATTTAACTATTGCTGAAATTAAAAATTATTGTAGA
AATACTATTCGTTCAAAGCCATATTACCAATCATAATATATTCCAATTAAATGTAAAAAA
AATAAAATAAAATAAAAAAACCAATATTTATGTTATTATTTTTTAAAAAAAAAAXXXXXX
XXXXAAACAATAATAAACACACATGTCAAGAATATATGAACAAATAGAAAAAGATGTTGA
AAAAATAATTGGTCAATATGATGGAGATAAAAATGGAGAAGTAACTATAAATGAAGCTAT
TGAATTTTTTAAAAGGATGGGATCAAAATATCCAGAGAAATGTGCAATTGTATTATTTAA
AATGTATGATCTAGATAATGAAGGAAAAATTTCATATGATGAAATTCAGGAAGAAATATT
TAAAAGATATCAAGATAAAGTAAGAGAGGATCAAATAAAACAATATTTTCAAGATGATAT
TGAAGCATTTCTATTGAGATATGATAAAAATAGAGATAATAGAATTGATTTTAAAGAACT
TGAACAATGTTTTGAAAGTATTGGTTCAGATCATCCAAAAGAAAATGCTAATCATATTTT
CACTGAAATTGATAAAAACAGAGATGGATATTTAACTATTGCTGAAATTAAAAATTATTG
TAGAAATACTATTCGTTCAAAGCCATATTACCAATCATAATATATTCCAATTAAATGAAA
AAAAATAAATAAATAAAAAACCAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
tiinthvkni*tnrkrc*knnwsi*wr*kwrsnyk*sy*if*kdgikisremcncii*nv
*sr**rknfi**nsgrni*kisr*skrgsnktifsr*y*sisiei**k*r**n*f*rt*t
mf*kywfrsskrkc*syfh*n**kqrwifnyc*n*kll*kyysfkailpiiiysn*m*kk
*NKIKKPIFMLLFFKKK---

---NNNKHTCQEYMNK*kkmlkk*lvnmmeikmek*l*mkllnflkgwdqniqrnvqlyy
lkcmi*imkekfhmmkfrkkylkdikik*erik*nnifkmilkhfy*dmikieiielilk
nlnnvlkvlvqiiqkkmliifslkliktemdi*lllklkiiveilfvqshitnhnifqln
ekk*inkkp
Translated Amino Acid sequence (All Frames) 
Frame A:
kq**thmsriyeqiekdvekiigqydgdkngevtineaieffkrmgskypekcaivlfkm
ydldnegkisydeiqeeifkryqdkvredqikqyfqddieafllrydknrdnridfkele
qcfesigsdhpkenanhifteidknrdgyltiaeiknycrntirskpyyqs*yipikckk
nkik*knqylcyyflkkk---

---kq**thmsriyeqiekdvekiigqydgdkngevtineaieffkrmgskypekcaivl
fkmydldnegkisydeiqeeifkryqdkvredqikqyfqddieafllrydknrdnridfk
eleqcfesigsdhpkenanhifteidknrdgyltiaeiknycrntirskpyyqs*yipik
*kkink*kt

Frame B:
nnnkhtcqeymnk*kkmlkk*lvnmmeikmek*l*mkllnflkgwdqniqrnvqlyylkc
mi*imkekfhmmkfrkkylkdikik*erik*nnifkmilkhfy*dmikieiielilknln
nvlkvlvqiiqkkmliifslkliktemdi*lllklkiiveilfvqshitnhnifqlnvkk
ik*nkktniyviif*kk---

---NNNKHTCQEYMNK*kkmlkk*lvnmmeikmek*l*mkllnflkgwdqniqrnvqlyy
lkcmi*imkekfhmmkfrkkylkdikik*erik*nnifkmilkhfy*dmikieiielilk
nlnnvlkvlvqiiqkkmliifslkliktemdi*lllklkiiveilfvqshitnhnifqln
ekk*inkkp

Frame C:
tiinthvkni*tnrkrc*knnwsi*wr*kwrsnyk*sy*if*kdgikisremcncii*nv
*sr**rknfi**nsgrni*kisr*skrgsnktifsr*y*sisiei**k*r**n*f*rt*t
mf*kywfrsskrkc*syfh*n**kqrwifnyc*n*kll*kyysfkailpiiiysn*m*kk
*NKIKKPIFMLLFFKKK---

---tiinthvkni*tnrkrc*knnwsi*wr*kwrsnyk*sy*if*kdgikisremcncii
*nv*sr**rknfi**nsgrni*kisr*skrgsnktifsr*y*sisiei**k*r**n*f*r
t*tmf*kywfrsskrkc*syfh*n**kqrwifnyc*n*kll*kyysfkailpiiiysn*m
kknk*iknq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL324 (AFL324Q) /CSM/AF/AFL3-A/AFL324Q.Seq.d/                       1059   0.0
SSD753 (SSD753Q) /CSM/SS/SSD7-C/SSD753Q.Seq.d/                       1033   0.0
SSA388 (SSA388Q) /CSM/SS/SSA3-D/SSA388Q.Seq.d/                       1023   0.0
SSA377 (SSA377Q) /CSM/SS/SSA3-D/SSA377Q.Seq.d/                        662   0.0
SSL489 (SSL489Q) /CSM/SS/SSL4-D/SSL489Q.Seq.d/                         36   1.2
SSL401 (SSL401Q) /CSM/SS/SSL4-A/SSL401Q.Seq.d/                         36   1.2
SSC248 (SSC248Q) /CSM/SS/SSC2-B/SSC248Q.Seq.d/                         36   1.2
SHF194 (SHF194Q) /CSM/SH/SHF1-D/SHF194Q.Seq.d/                         36   1.2
SHE183 (SHE183Q) /CSM/SH/SHE1-D/SHE183Q.Seq.d/                         36   1.2
CHK506 (CHK506Q) /CSM/CH/CHK5-A/CHK506Q.Seq.d/                         36   1.2
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AB070451|AB070451.1 Dictyostelium discoideum cbpH mRNA for calcium binding protein CBP8, complete cds, clone:SSD753.  1051   0.0    2
AE017263|AE017263.1 Mesoplasma florum L1 complete genome.          38   2e-20  38
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           44   9e-13  30
AB063522|AB063522.2 Wigglesworthia brevipalpis DNA, complete genome, section 2/2.    36   3e-12  25
AE014839|AE014839.1 Plasmodium falciparum 3D7 chromosome 11 section 4 of 8 of the complete sequence.    34   1e-11  23
AL844506|AL844506.1 Plasmodium falciparum chromosome  7.           38   2e-11  31
BD061520|BD061520.1 Genome DNA of symbiotic bacteria of aphid.     34   2e-11  32
AR409405|AR409405.1 Sequence 1 from patent US 6632935.             34   2e-11  32
AR271569|AR271569.1 Sequence 1 from patent US 6503729.             44   3e-11  36
BX842643|BX842643.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 2/4.    32   6e-11  28
dna update 2004. 8.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q966Q9) RecName: Full=Calcium-binding protein H; AltName: Full=...   341   3e-92
(P54653) RecName: Full=Calcium-binding protein 2;  &S62881(S6288...    92   4e-17
(Q966R0) RecName: Full=Calcium-binding protein I; AltName: Full=...    81   6e-14
(Q54QT7) RecName: Full=Calcium-binding protein C; AltName: Full=...    64   1e-08
CR932101_1(CR932101|pid:none) Paramecium tetraurelia centrin-rel...    64   1e-08
AJ297597_1(AJ297597|pid:none) Dictyostelium discoideum mRNA for ...    64   1e-08
(Q54RF4) RecName: Full=Calcium-binding protein 4a;                     64   1e-08
(Q54QT9) RecName: Full=Calcium-binding protein F; AltName: Full=...    62   4e-08
CR933337_1(CR933337|pid:none) Paramecium tetraurelia centrin-rel...    62   4e-08
CR932105_1(CR932105|pid:none) Paramecium tetraurelia centrin-rel...    62   4e-08
protein update 2009. 4.25
PSORT 

 psg: 0.99  gvh: 0.14  alm: 0.65  top: 0.50  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.12  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 20.0 %: cytoplasmic
	 16.0 %: cytoskeletal
	 12.0 %: mitochondrial

>> prediction for AFL324 is nuc
5' end seq. ID AFL324F
5' end seq. 
>AFL324F.Seq
AAACAATAATAAACACACATGTCAAGAATATATGAACAAATAGAAAAAGATGTTGAAAAA
ATAATTGGTCAATATGATGGAGATAAAAATGGAGAAGTAACTATAAATGAAGCTATTGAA
TTTTTTAAAAGGATGGGATCAAAATATCCAGAGAAATGTGCAATTGTATTATTTAAAATG
TATGATCTAGATAATGAAGGAAAAATTTCATATGATGAAATTCAGGAAGAAATATTTAAA
AGATATCAAGATAAAGTAAGAGAGGATCAAATAAAACAATATTTTCAAGATGATATTGAA
GCATTTCTATTGAGATATGATAAAAATAGAGATAATAGAATTGATTTTAAAGAACTTGAA
CAATGTTTTGAAAGTATTGGTTCAGATCATCCAAAAGAAAATGCTAATCATATTTTCACT
GAAATTGATAAAAACAGAGATGGATATTTAACTATTGCTGAAATTAAAAATTATTGTAGA
AATACTATTCGTTCAAAGCCATATTACCAATCATAATATATTCCAATTAAATGTAAAAAA
AATAAAATAAAATAAAAAAACCAATATTTATGTTATTATTTTTTAAAAAAAAAA------
----
Length of 5' end seq. 594
3' end seq. ID AFL324Z
3' end seq. 
>AFL324Z.Seq
----------AAACAATAATAAACACACATGTCAAGAATATATGAACAAATAGAAAAAGA
TGTTGAAAAAATAATTGGTCAATATGATGGAGATAAAAATGGAGAAGTAACTATAAATGA
AGCTATTGAATTTTTTAAAAGGATGGGATCAAAATATCCAGAGAAATGTGCAATTGTATT
ATTTAAAATGTATGATCTAGATAATGAAGGAAAAATTTCATATGATGAAATTCAGGAAGA
AATATTTAAAAGATATCAAGATAAAGTAAGAGAGGATCAAATAAAACAATATTTTCAAGA
TGATATTGAAGCATTTCTATTGAGATATGATAAAAATAGAGATAATAGAATTGATTTTAA
AGAACTTGAACAATGTTTTGAAAGTATTGGTTCAGATCATCCAAAAGAAAATGCTAATCA
TATTTTCACTGAAATTGATAAAAACAGAGATGGATATTTAACTATTGCTGAAATTAAAAA
TTATTGTAGAAATACTATTCGTTCAAAGCCATATTACCAATCATAATATATTCCAATTAA
ATGAAAAAAAATAAATAAATAAAAAACCAA
Length of 3' end seq. 560
Connected seq. ID AFL324P
Connected seq. 
>AFL324P.Seq
AAACAATAATAAACACACATGTCAAGAATATATGAACAAATAGAAAAAGATGTTGAAAAA
ATAATTGGTCAATATGATGGAGATAAAAATGGAGAAGTAACTATAAATGAAGCTATTGAA
TTTTTTAAAAGGATGGGATCAAAATATCCAGAGAAATGTGCAATTGTATTATTTAAAATG
TATGATCTAGATAATGAAGGAAAAATTTCATATGATGAAATTCAGGAAGAAATATTTAAA
AGATATCAAGATAAAGTAAGAGAGGATCAAATAAAACAATATTTTCAAGATGATATTGAA
GCATTTCTATTGAGATATGATAAAAATAGAGATAATAGAATTGATTTTAAAGAACTTGAA
CAATGTTTTGAAAGTATTGGTTCAGATCATCCAAAAGAAAATGCTAATCATATTTTCACT
GAAATTGATAAAAACAGAGATGGATATTTAACTATTGCTGAAATTAAAAATTATTGTAGA
AATACTATTCGTTCAAAGCCATATTACCAATCATAATATATTCCAATTAAATGTAAAAAA
AATAAAATAAAATAAAAAAACCAATATTTATGTTATTATTTTTTAAAAAAAAAA------
----AAACAATAATAAACACACATGTCAAGAATATATGAACAAATAGAAAAAGATGTTGA
AAAAATAATTGGTCAATATGATGGAGATAAAAATGGAGAAGTAACTATAAATGAAGCTAT
TGAATTTTTTAAAAGGATGGGATCAAAATATCCAGAGAAATGTGCAATTGTATTATTTAA
AATGTATGATCTAGATAATGAAGGAAAAATTTCATATGATGAAATTCAGGAAGAAATATT
TAAAAGATATCAAGATAAAGTAAGAGAGGATCAAATAAAACAATATTTTCAAGATGATAT
TGAAGCATTTCTATTGAGATATGATAAAAATAGAGATAATAGAATTGATTTTAAAGAACT
TGAACAATGTTTTGAAAGTATTGGTTCAGATCATCCAAAAGAAAATGCTAATCATATTTT
CACTGAAATTGATAAAAACAGAGATGGATATTTAACTATTGCTGAAATTAAAAATTATTG
TAGAAATACTATTCGTTCAAAGCCATATTACCAATCATAATATATTCCAATTAAATGAAA
AAAAATAAATAAATAAAAAACCAA
Length of connected seq. 1154
Full length Seq ID -
Full length Seq. -
Length of full length seq. -